| General information | |||
| TC010381 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 24979517..24992013 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010381 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07067 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
egg chamber mostly not present - Number of animals showing the phenotype: 2
egg chamber structure irregular - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC010381 RA
MSAGMPEIG SKISLISKA DIRYEGRLF TVDPKECTI ALSSVRSFG TEDRETPYP VPAQNQVYD YILFRGSDI KDIRVVNNV AQPLNDPAI MQLSVPPTL GQQQFQNHP MMGHPLGPQ MGQFASGYG AMGSMADII GGGSRSTTP ASLRSRKSP TNDQGTQAG GAGKRDDKK TPKPIQPPG QRNQQRKST SQERKNSRG DGPQDYQRN QNRQPQMRT QNQGGPQGS HLGPQQITQ QRGGWTNRG PMRQRGRPR GGFKPNQGQ PTPGNKAKN TLKFENDYD FEQANTEFE ELRSQLAKV KVEETSKTE LNGDVDKKD DSGNETGAG ENEPEEEHE VYYDKSKSF FDKISCEAV ERAKGKSQR TDWRTERKL NSETFGVAA TRRGNFRGR GGFRGMGYR GGYRGGFRP QQRRDQQQQ QQQGNRPGQ NQQNDVQRP TTNSQPPAS VPAATGK
- >TC010381 RB
MSAGMPEIG SKISLISKA DIRYEGRLF TVDPKECTI ALSSVRSFG TEDRETPYP VPAQNQVYD YILFRGSDI KDIRVVNNV AQPLNDPAI MQLSVPPTL GQQQFQNHP MMGHPLGPQ MGQFASGYG AMGSMAGSL LDIIGGGSR STTPASLRS RKSPTNDQG TQAGGAGKR DDKKTPKPI QPPGQRNQQ RKSTSQERK NSRGDGPQD YQRNQNRQP QMRTQNQGG PQGSHLGPQ QITQQRGGW TNRGPMRQR GRPRGGFKP NQGQPTPGN KAKNTLKFE NDYDFEQAN TEFEELRSQ LAKVKVEET SKTELNGDV DKKDDSGNE TGAGENEPE EEHEVYYDK SKSFFDKIS CEAVERAKG KSQRTDWRT ERKLNSETF GVAATRRGN FRGRGGFRG MGYRGGYRG GFRPQQRRD QQQQQQQGN RPGQNQQND VQRPTTNSQ PPASVPAAT GK
- >TC010381 RC
MSAGMPEIG SKISLISKA DIRYEGRLF TVDPKECTI ALSSVRSFG TEDRETPYP VPAQNQVYD YILFRGSDI KDIRVVNNV AQPLNDPAI MQLSVPPTL GQQQFQNHP MMGHPLGPQ MGQFASGYG AMGSMAGLS SGMVPGMGM SRDPRGLAG NKPNDLIIP GTEQPQVLS PAVEPPRPS DHDIIGGGS RSTTPASLR SRKSPTNDQ GTQAGGAGK RDDKKTPKP IQPPGQRNQ QRKSTSQER KNSRGDGPQ DYQRNQNRQ PQMRTQNQG GPQGSHLGP QQITQQRGG WTNRGPMRQ RGRPRGGFK PNQGQPTPG NKAKNTLKF ENDYDFEQA NTEFEELRS QLAKVKVEE TSKTELNGD VDKKDDSGN ETGAGENEP EEEHEVYYD KSKSFFDKI SCEAVERAK GKSQRTDWR TERKLNSET FGVAATRRG NFRGRGGFR GMGYRGGYR GGFRPQQRR DQQQQQQQG NRPGQNQQN DVQRPTTNS QPPASVPAA TGK
- >TC010381 RA
atgagtgct ggtatgccg gagattggc tccaaaata agtctaata tcgaaagcc gatatccgg tacgaagga cgattattt acagtagac cccaaagaa tgtacaatt gccctatct tccgttcgc tcatttggt actgaagac agggagacg ccttacccc gtccctgcc cagaaccaa gtctatgac tacatttta ttccgcggc tctgacatc aaagatatt cgagttgtg aataatgtt gctcaacct cttaacgat ccggcgatt atgcagctg tcggttcct ccaacattg ggacagcag cagttccag aaccatccg atgatgggg caccccctg gggccccaa atgggacaa tttgcgtcg ggttatgga gcgatgggt tcaatggca gacataatc ggaggcggt tcaaggagt acgacacca gctagttta aggtcgcgt aaatccccc actaacgat caaggtaca caagctggt ggagcgggt aaacgagat gacaagaag actccgaaa ccaattcaa cccccaggt caacgcaat caacagagg aaaagtact tcacaagaa aggaagaat tcaagagga gatggccca caggattat caaagaaat caaaatagg caacctcaa atgcgcaca cagaatcaa ggaggaccc caaggatct catctcggc ccgcaacaa atcacccaa caaaggggc ggttggaca aatcgcggt cctatgagg cagagggga aggccaaga ggaggcttc aaacctaat caaggacag ccgacacca ggaaataaa gcaaagaat actttgaag tttgaaaat gattacgac tttgaacaa gcgaatacg gaatttgaa gagttgagg agtcagtta gctaaagta aaagttgaa gaaactagt aagactgaa ttgaacggt gatgtagac aaaaaggat gattcaggc aacgaaacg ggcgccgga gaaaatgag cctgaagaa gaacacgaa gtttactat gacaagagc aagtctttc tttgataaa atttcatgt gaggctgtg gaacgtgcg aagggcaaa tcacagcga acggattgg cgtacggaa cgcaaactt aactccgaa acttttgga gttgccgca actcgaaga ggaaatttc cgaggtcgc ggaggattc cgaggcatg ggatacagg ggaggatac cgtgggggt ttcaggccc caacagcgt agagatcaa caacaacaa caacaacaa ggcaatcga cccggtcag aaccaacaa aacgacgtg caaagacca acaacaaac agccaacct ccggcgtct gtgcctgcg gcgacagga aaataa
- >TC010381 RB
atgagtgct ggtatgccg gagattggc tccaaaata agtctaata tcgaaagcc gatatccgg tacgaagga cgattattt acagtagac cccaaagaa tgtacaatt gccctatct tccgttcgc tcatttggt actgaagac agggagacg ccttacccc gtccctgcc cagaaccaa gtctatgac tacatttta ttccgcggc tctgacatc aaagatatt cgagttgtg aataatgtt gctcaacct cttaacgat ccggcgatt atgcagctg tcggttcct ccaacattg ggacagcag cagttccag aaccatccg atgatgggg caccccctg gggccccaa atgggacaa tttgcgtcg ggttatgga gcgatgggt tcaatggca ggatctctt ttagacata atcggaggc ggttcaagg agtacgaca ccagctagt ttaaggtcg cgtaaatcc cccactaac gatcaaggt acacaagct ggtggagcg ggtaaacga gatgacaag aagactccg aaaccaatt caaccccca ggtcaacgc aatcaacag aggaaaagt acttcacaa gaaaggaag aattcaaga ggagatggc ccacaggat tatcaaaga aatcaaaat aggcaacct caaatgcgc acacagaat caaggagga ccccaagga tctcatctc ggcccgcaa caaatcacc caacaaagg ggcggttgg acaaatcgc ggtcctatg aggcagagg ggaaggcca agaggaggc ttcaaacct aatcaagga cagccgaca ccaggaaat aaagcaaag aatactttg aagtttgaa aatgattac gactttgaa caagcgaat acggaattt gaagagttg aggagtcag ttagctaaa gtaaaagtt gaagaaact agtaagact gaattgaac ggtgatgta gacaaaaag gatgattca ggcaacgaa acgggcgcc ggagaaaat gagcctgaa gaagaacac gaagtttac tatgacaag agcaagtct ttctttgat aaaatttca tgtgaggct gtggaacgt gcgaagggc aaatcacag cgaacggat tggcgtacg gaacgcaaa cttaactcc gaaactttt ggagttgcc gcaactcga agaggaaat ttccgaggt cgcggagga ttccgaggc atgggatac aggggagga taccgtggg ggtttcagg ccccaacag cgtagagat caacaacaa caacaacaa caaggcaat cgacccggt cagaaccaa caaaacgac gtgcaaaga ccaacaaca aacagccaa cctccggcg tctgtgcct gcggcgaca ggaaaataa
- >TC010381 RC
atgagtgct ggtatgccg gagattggc tccaaaata agtctaata tcgaaagcc gatatccgg tacgaagga cgattattt acagtagac cccaaagaa tgtacaatt gccctatct tccgttcgc tcatttggt actgaagac agggagacg ccttacccc gtccctgcc cagaaccaa gtctatgac tacatttta ttccgcggc tctgacatc aaagatatt cgagttgtg aataatgtt gctcaacct cttaacgat ccggcgatt atgcagctg tcggttcct ccaacattg ggacagcag cagttccag aaccatccg atgatgggg caccccctg gggccccaa atgggacaa tttgcgtcg ggttatgga gcgatgggt tcaatggca ggtctgtcg agtggcatg gtaccgggt atgggtatg tcgagagat cccagaggt ctcgctggt aataaacca aatgattta attataccg ggtacggaa caaccacaa gtcctatct cctgcagtt gaaccgcct cgaccaagc gatcacgac ataatcgga ggcggttca aggagtacg acaccagct agtttaagg tcgcgtaaa tcccccact aacgatcaa ggtacacaa gctggtgga gcgggtaaa cgagatgac aagaagact ccgaaacca attcaaccc ccaggtcaa cgcaatcaa cagaggaaa agtacttca caagaaagg aagaattca agaggagat ggcccacag gattatcaa agaaatcaa aataggcaa cctcaaatg cgcacacag aatcaagga ggaccccaa ggatctcat ctcggcccg caacaaatc acccaacaa aggggcggt tggacaaat cgcggtcct atgaggcag aggggaagg ccaagagga ggcttcaaa cctaatcaa ggacagccg acaccagga aataaagca aagaatact ttgaagttt gaaaatgat tacgacttt gaacaagcg aatacggaa tttgaagag ttgaggagt cagttagct aaagtaaaa gttgaagaa actagtaag actgaattg aacggtgat gtagacaaa aaggatgat tcaggcaac gaaacgggc gccggagaa aatgagcct gaagaagaa cacgaagtt tactatgac aagagcaag tctttcttt gataaaatt tcatgtgag gctgtggaa cgtgcgaag ggcaaatca cagcgaacg gattggcgt acggaacgc aaacttaac tccgaaact tttggagtt gccgcaact cgaagagga aatttccga ggtcgcgga ggattccga ggcatggga tacagggga ggataccgt gggggtttc aggccccaa cagcgtaga gatcaacaa caacaacaa caacaaggc aatcgaccc ggtcagaac caacaaaac gacgtgcaa agaccaaca acaaacagc caacctccg gcgtctgtg cctgcggcg acaggaaaa taa
- >TC010381 RA
tgcgtcatt cgctccagc tgttttggg tacgccatt tgagaatcg tattttcga aataggttg agttaaatg ttaatcgtt taaaatcct cgtcatcct gttcgtaaa gtgatttat tcagaatga gtgctggta tgccggaga ttggctcca aaataagtc taatatcga aagccgata tccggtacg aaggacgat tatttacag tagacccca aagaatgta caattgccc tatcttccg ttcgctcat ttggtactg aagacaggg agacgcctt accccgtcc ctgcccaga accaagtct atgactaca ttttattcc gcggctctg acatcaaag atattcgag ttgtgaata atgttgctc aacctctta acgatccgg cgattatgc agctgtcgg ttcctccaa cattgggac agcagcagt tccagaacc atccgatga tggggcacc ccctggggc cccaaatgg gacaatttg cgtcgggtt atggagcga tgggttcaa tggcagaca taatcggag gcggttcaa ggagtacga caccagcta gtttaaggt cgcgtaaat cccccacta acgatcaag gtacacaag ctggtggag cgggtaaac gagatgaca agaagactc cgaaaccaa ttcaacccc caggtcaac gcaatcaac agaggaaaa gtacttcac aagaaagga agaattcaa gaggagatg gcccacagg attatcaaa gaaatcaaa ataggcaac ctcaaatgc gcacacaga atcaaggag gaccccaag gatctcatc tcggcccgc aacaaatca cccaacaaa ggggcggtt ggacaaatc gcggtccta tgaggcaga ggggaaggc caagaggag gcttcaaac ctaatcaag gacagccga caccaggaa ataaagcaa agaatactt tgaagtttg aaaatgatt acgactttg aacaagcga atacggaat ttgaagagt tgaggagtc agttagcta aagtaaaag ttgaagaaa ctagtaaga ctgaattga acggtgatg tagacaaaa aggatgatt caggcaacg aaacgggcg ccggagaaa atgagcctg aagaagaac acgaagttt actatgaca agagcaagt ctttctttg ataaaattt catgtgagg ctgtggaac gtgcgaagg gcaaatcac agcgaacgg attggcgta cggaacgca aacttaact ccgaaactt ttggagttg ccgcaactc gaagaggaa atttccgag gtcgcggag gattccgag gcatgggat acaggggag gataccgtg ggggtttca ggccccaac agcgtagag atcaacaac aacaacaac aacaaggca atcgacccg gtcagaacc aacaaaacg acgtgcaaa gaccaacaa caaacagcc aacctccgg cgtctgtgc ctgcggcga caggaaaat aaagtacta actgatctg actagataa acaaacata aacagttgc tgacacagg aacatggag acatgactg gtttaattt attggggga aggatgtgg atttagtta actgtgaac tgtgattaa aagtacttg attattttg taaattgat ttcggagtc aagcgtgaa ttgtacgaa aagcaatgc ttctttttg agtttcctg gtgtcagta ttaaagtag gttagttaa ggtgtacct taagtgata aatatttta ttttatata taaaaaagg gcagataaa aaaatttta gtaatgtat atttttact gccaataga aattaatat taatttttt atatttctt gtagcttta tatggttag aacaatgtt tttttcttt aaattatac acttaattt ttattttta atcgggtca aaataaaat tctaatcaa ttgttctaa ctggttgta ataatataa tttgccaaa tggt
- >TC010381 RB
tgcgtcatt cgctccagc tgttttggg tacgccatt tgagaatcg tattttcga aataggttg agttaaatg ttaatcgtt taaaatcct cgtcatcct gttcgtaaa gtgatttat tcagaatga gtgctggta tgccggaga ttggctcca aaataagtc taatatcga aagccgata tccggtacg aaggacgat tatttacag tagacccca aagaatgta caattgccc tatcttccg ttcgctcat ttggtactg aagacaggg agacgcctt accccgtcc ctgcccaga accaagtct atgactaca ttttattcc gcggctctg acatcaaag atattcgag ttgtgaata atgttgctc aacctctta acgatccgg cgattatgc agctgtcgg ttcctccaa cattgggac agcagcagt tccagaacc atccgatga tggggcacc ccctggggc cccaaatgg gacaatttg cgtcgggtt atggagcga tgggttcaa tggcaggat ctcttttag acataatcg gaggcggtt caaggagta cgacaccag ctagtttaa ggtcgcgta aatccccca ctaacgatc aaggtacac aagctggtg gagcgggta aacgagatg acaagaaga ctccgaaac caattcaac ccccaggtc aacgcaatc aacagagga aaagtactt cacaagaaa ggaagaatt caagaggag atggcccac aggattatc aaagaaatc aaaataggc aacctcaaa tgcgcacac agaatcaag gaggacccc aaggatctc atctcggcc cgcaacaaa tcacccaac aaaggggcg gttggacaa atcgcggtc ctatgaggc agaggggaa ggccaagag gaggcttca aacctaatc aaggacagc cgacaccag gaaataaag caaagaata ctttgaagt ttgaaaatg attacgact ttgaacaag cgaatacgg aatttgaag agttgagga gtcagttag ctaaagtaa aagttgaag aaactagta agactgaat tgaacggtg atgtagaca aaaaggatg attcaggca acgaaacgg gcgccggag aaaatgagc ctgaagaag aacacgaag tttactatg acaagagca agtctttct ttgataaaa tttcatgtg aggctgtgg aacgtgcga agggcaaat cacagcgaa cggattggc gtacggaac gcaaactta actccgaaa cttttggag ttgccgcaa ctcgaagag gaaatttcc gaggtcgcg gaggattcc gaggcatgg gatacaggg gaggatacc gtgggggtt tcaggcccc aacagcgta gagatcaac aacaacaac aacaacaag gcaatcgac ccggtcaga accaacaaa acgacgtgc aaagaccaa caacaaaca gccaacctc cggcgtctg tgcctgcgg cgacaggaa aataaagta ctaactgat ctgactaga taaacaaac ataaacagt tgctgacac aggaacatg gagacatga ctggtttaa tttattggg ggaaggatg tggatttag ttaactgtg aactgtgat taaaagtac ttgattatt ttgtaaatt gatttcgga gtcaagcgt gaattgtac gaaaagcaa tgcttcttt ttgagtttc ctggtgtca gtattaaag taggttagt taaggtgta ccttaagtg ataaatatt ttattttat atataaaaa agggcagat aaaaaaatt ttagtaatg tatattttt actgccaat agaaattaa tattaattt tttatattt cttgtagct ttatatggt tagaacaat gtttttttc tttaaatta tacacttaa tttttattt ttaatcggg tcaaaataa aattctaat caattgttc taactggtt gtaataata taatttgcc aaatggt
- >TC010381 RC
tgcgtcatt cgctccagc tgttttggg tacgccatt tgagaatcg tattttcga aataggttg agttaaatg ttaatcgtt taaaatcct cgtcatcct gttcgtaaa gtgatttat tcagaatga gtgctggta tgccggaga ttggctcca aaataagtc taatatcga aagccgata tccggtacg aaggacgat tatttacag tagacccca aagaatgta caattgccc tatcttccg ttcgctcat ttggtactg aagacaggg agacgcctt accccgtcc ctgcccaga accaagtct atgactaca ttttattcc gcggctctg acatcaaag atattcgag ttgtgaata atgttgctc aacctctta acgatccgg cgattatgc agctgtcgg ttcctccaa cattgggac agcagcagt tccagaacc atccgatga tggggcacc ccctggggc cccaaatgg gacaatttg cgtcgggtt atggagcga tgggttcaa tggcaggtc tgtcgagtg gcatggtac cgggtatgg gtatgtcga gagatccca gaggtctcg ctggtaata aaccaaatg atttaatta taccgggta cggaacaac cacaagtcc tatctcctg cagttgaac cgcctcgac caagcgatc acgacataa tcggaggcg gttcaagga gtacgacac cagctagtt taaggtcgc gtaaatccc ccactaacg atcaaggta cacaagctg gtggagcgg gtaaacgag atgacaaga agactccga aaccaattc aacccccag gtcaacgca atcaacaga ggaaaagta cttcacaag aaaggaaga attcaagag gagatggcc cacaggatt atcaaagaa atcaaaata ggcaacctc aaatgcgca cacagaatc aaggaggac cccaaggat ctcatctcg gcccgcaac aaatcaccc aacaaaggg gcggttgga caaatcgcg gtcctatga ggcagaggg gaaggccaa gaggaggct tcaaaccta atcaaggac agccgacac caggaaata aagcaaaga atactttga agtttgaaa atgattacg actttgaac aagcgaata cggaatttg aagagttga ggagtcagt tagctaaag taaaagttg aagaaacta gtaagactg aattgaacg gtgatgtag acaaaaagg atgattcag gcaacgaaa cgggcgccg gagaaaatg agcctgaag aagaacacg aagtttact atgacaaga gcaagtctt tctttgata aaatttcat gtgaggctg tggaacgtg cgaagggca aatcacagc gaacggatt ggcgtacgg aacgcaaac ttaactccg aaacttttg gagttgccg caactcgaa gaggaaatt tccgaggtc gcggaggat tccgaggca tgggataca ggggaggat accgtgggg gtttcaggc cccaacagc gtagagatc aacaacaac aacaacaac aaggcaatc gacccggtc agaaccaac aaaacgacg tgcaaagac caacaacaa acagccaac ctccggcgt ctgtgcctg cggcgacag gaaaataaa gtactaact gatctgact agataaaca aacataaac agttgctga cacaggaac atggagaca tgactggtt taatttatt gggggaagg atgtggatt tagttaact gtgaactgt gattaaaag tacttgatt attttgtaa attgatttc ggagtcaag cgtgaattg tacgaaaag caatgcttc tttttgagt ttcctggtg tcagtatta aagtaggtt agttaaggt gtaccttaa gtgataaat attttattt tatatataa aaaagggca gataaaaaa attttagta atgtatatt tttactgcc aatagaaat taatattaa ttttttata tttcttgta gctttatat ggttagaac aatgttttt ttctttaaa ttatacact taattttta tttttaatc gggtcaaaa taaaattct aatcaattg ttctaactg gttgtaata atataattt gccaaatgg t
