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Gene information
General information
TC032592
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010344
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24622676..24624625
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032592
    iBeetle screen iB_07064

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032592 RA
      MVGLSDSSH KLSQLDFVI AGGGSGFIT 
      RMLCQPLDV LKIRFQLQV EPILKSNVS 
      KYQSVFHAA NLIVKEEGV KALWKGHVP 
      AQLLSISYG VAQFWSFEV LTKQVSRIN 
      LSPTFSPMV NFTCGALAG CYATLASFP 
      FDVVRTRLV AQSENRKVY SEVFGPRFY 
      KLLLMQGHS SCVTNYLII FINI
    CDS sequence
    • >TC032592 RA
      atggtgggg ttgagtgat tcctctcac aaactgtcg 
      cagctcgat tttgtgatt gctggtgga ggttcaggc 
      tttattaca cgaatgtta tgtcagcct ttagacgtg 
      cttaaaata agatttcaa ctgcaagtt gaaccgatt 
      ttgaaaagt aacgtttca aaataccag tcagtgttt 
      cacgccgct aatttaatt gtcaaagag gaaggggtg 
      aaggcactg tggaagggt catgtccct gcacaatta 
      ctctcaata agttacgga gttgcccag ttttggagc 
      ttcgaagtg ttaaccaag caagtctct agaattaat 
      ttatcacca actttttct cctatggtt aatttcact 
      tgtggcgcc cttgcaggt tgttacgcc actcttgcg 
      tcatttccc tttgatgta gttaggacc agattagtt 
      gcacaaagt gaaaaccga aaagtctat tctgaggta 
      tttggccca cgtttttac aagttgctc ctcatgcag 
      gggcacagt tcatgtgtt acaaattat ttgataata 
      tttataaac atttaa
    mRNA sequence
    • >TC032592 RA
      aaaaacaca agtgttaag ttgaggtta cgcaaatgt 
      caaactgac gtctgtcac gaggtgagg ttatatcac 
      gaaacaaaa aatggaaaa ctcgctctt gttggtgtt 
      taaatgata gacttgtca cttgtattt cctgggggt 
      tgatgcaat ggtggggtt gagtgattc ctctcacaa 
      actgtcgca gctcgattt tgtgattgc tggtggagg 
      ttcaggctt tattacacg aatgttatg tcagccttt 
      agacgtgct taaaataag atttcaact gcaagttga 
      accgatttt gaaaagtaa cgtttcaaa ataccagtc 
      agtgtttca cgccgctaa tttaattgt caaagagga 
      aggggtgaa ggcactgtg gaagggtca tgtccctgc 
      acaattact ctcaataag ttacggagt tgcccagtt 
      ttggagctt cgaagtgtt aaccaagca agtctctag 
      aattaattt atcaccaac tttttctcc tatggttaa 
      tttcacttg tggcgccct tgcaggttg ttacgccac 
      tcttgcgtc atttccctt tgatgtagt taggaccag 
      attagttgc acaaagtga aaaccgaaa agtctattc 
      tgaggtatt tggcccacg tttttacaa gttgctcct 
      catgcaggg gcacagttc atgtgttac aaattattt 
      gataatatt tataaacat ttaatcaat tcacaaaat 
      acaacgtta actagtagc ttagtgtcg ggaagtttg 
      gcaggtttg tgtgcaaaa actgtagtc tatccgttt 
      gatttggct aaaaaaaga ttacaaatt cagggtttt 
      gaacaaggg agggccgaa tttggacaa tttttcaag 
      tgtcaggga cttaatgat tgtctcatt agaatctac 
      aaagttgaa ggtccttca ggccttttt aaaggcctt 
      agtcctagt ctcattaag gctgttgta acaacagct 
      ttacacttt agtagttat gaattaatt tgtaaagct 
      ttggcaaag aggaagaaa taatattta tttgcatta 
      gtgtggtat tatagaact tataaattt ttattttta 
      tttaaaaat acctattta aagatttat taaaaattt 
      gttaaagtt tttgttaat tgccgcgta cgtaactac 
      agagtgatc aagaaagac tgcataaat tggcaacac 
      ttaatttta gttggtaca cctggagtt tatacctac 
      tctccagtt attgaactc gaagaaagt aaaagtaac 
      aatttttag tgcttttcc ggtttgctt tcacgaatt 
      ttctaatta atgtatctt tctgtctct tttaccaga 
      attttgaaa taaattgat aatacaaaa gccaattca 
      gaattccca acttaaatt ggtgatcaa tttgtatct 
      acataaaat ctaggtcgt ttaaaagtt aagtattta 
      ttaatcttt attgctttt tcttgttat acttacgag 
      tatttctga acaattatg ttttttttg ttatttctt 
      attcaagtt tattgtttc tgaattgct cacaaaaaa 
      taagtacat aatcgttat tttattctt attctttaa 
      aatggcttt atgttttaa gaaaaaaat gtgttgtgt 
      tagtcggct gataatttt tttttaatc atacatcaa 
      tgaatcctg tgatcgttg ttatgtgtt cactcatca 
      ctagtgtga tattattgt tttgtttgt ttaatattt 
      attgtagta cttaaatga atctgtacc aaaaatgtg 
      aagactaat aaaaatagt aatatatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY