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Gene information
General information
TC010345 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24496380..24497443
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010345
    iBeetle screen iB_07063

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010345 RA
      MNVVGMGMI PQPNPQQPQ QQQQQQPHQ 
      PLDNISKIK TLIGPLRES LSNTIKTAA 
      QTLNQNSQV DAGSLKGVD VPIPRFDKN 
      LEEFYSICD QIELHLKTS IKCIGQAES 
      SNRYLHVPV IPHRSENIG INENMLSYP 
      QFLATASAQ VAYAKEIHD TLIAAAQNI 
      SPSD
    CDS sequence
    • >TC010345 RA
      atgaatgtg gtgggaatg ggaatgatc ccccaacca 
      aatccgcaa cagccgcag caacaacaa cagcaacag 
      ccccaccag cccctggac aacatttcc aaaattaaa 
      accttgatt gggccttta agggagtcg ctttctaat 
      accattaag acagcggca caaacttta aaccaaaat 
      agtcaagtg gatgcgggc tctttgaaa ggggttgat 
      gtgccaata ccacgattc gataaaaat ctagaagaa 
      ttttactca atatgtgac caaattgag ttacatcta 
      aaaacttcg ataaaatgc attggccaa gcggaaagt 
      agcaatcga tatttgcac gttcctgtg attccccac 
      agaagcgag aatattgga attaatgaa aatatgttg 
      tcatatcct caatttttg gccactgct agtgctcaa 
      gtggcgtat gctaaagaa attcatgat acattaatt 
      gcagcggct caaaatatt tctccttct gattga
    mRNA sequence
    • >TC010345 RA
      cacagcgat ctgtaatct gtactgttg ttgacaact 
      aatctaacc tttattttt ttagtaaaa caatttgat 
      tattttgat tagaaaata tcgctataa aataacagc 
      catgaatgt ggtgggaat gggaatgat cccccaacc 
      aaatccgca acagccgca gcaacaaca acagcaaca 
      gccccacca gcccctgga caacatttc caaaattaa 
      aaccttgat tgggccttt aagggagtc gctttctaa 
      taccattaa gacagcggc acaaacttt aaaccaaaa 
      tagtcaagt ggatgcggg ctctttgaa aggggttga 
      tgtgccaat accacgatt cgataaaaa tctagaaga 
      attttactc aatatgtga ccaaattga gttacatct 
      aaaaacttc gataaaatg cattggcca agcggaaag 
      tagcaatcg atatttgca cgttcctgt gattcccca 
      cagaagcga gaatattgg aattaatga aaatatgtt 
      gtcatatcc tcaattttt ggccactgc tagtgctca 
      agtggcgta tgctaaaga aattcatga tacattaat 
      tgcagcggc tcaaaatat ttctccttc tgattgacg 
      tttccagca attttaatt ttttgtaaa ttatttatt 
      gtatcgtct ttattttat accctgttt tcaattaag 
      tttctgatt aaagttgca tattatgtg aaatttaca 
      cagtcatag cccacttga cgaattgta atagcataa 
      ataattcat tgttgccta ctaatttac caagtcaca 
      agtcggttt gatcagaaa tttagttga aacctcaat 
      aatggttaa atatatttt tttaattta acagtgata 
      aggattgat aagtgtgtg tattcaaat aaagaattt 
      tctcaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY