Status
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Gene information
General information
TC032584 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24139270..24146727
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032584
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07061

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032584 RA
      MSRKSGTVP LHEACKPKL IAQVDQKKG GVNTVVMTN 
      YKCEKNEVT VPSANGSTV PLVNAARGD EELGDYDPF 
      AHRKLDHPT SDLDTLIHL LKGSLGSGI LAMPMAFAN 
      AGLFFGLFC TFAIGSICT YCVHILVKS AHALCRRNR 
      VPSLGYAEV AEAAFLAGP QSIRPWAKF AKAMINLFL 
      VIDLLGCCC VYIMFVAEN IKQVVDNYT PKDTHQDIK 
      IYMAALLPL LILINLIRN LKYLSPLSM IANILVASG 
      MGITYYYLI NEMPSLDER PKIVEFTKL PKFFGTVIF 
      ALEGIGVVM PLENNMKTP THFIGCPGV LNIGMFVVV 
      LLYATTGFL GYLKYGTHT QPSITLNLP ETEPLGQSV 
      KLMIAVAVF FTYALQFYV PMEIIWKNL KGLFNKRPN 
      LSEYSIRIS LVILTAVIA ILVPDLEGL ISLVGALCL 
      SMLGLIFPA VIDLVTFWE DPGFGRLNW RLWKNVLLI 
      FFGFVGFAT GTYVSIQGI IERANSS
    CDS sequence
    • >TC032584 RA
      atgtctcga aaatctgga acggtacct ctacatgag 
      gcatgcaag cctaaactc atagctcaa gtagaccag 
      aaaaagggt ggtgttaac acagtagtg atgactaat 
      tataaatgt gaaaaaaat gaagtgact gtgccttcg 
      gccaatggc tcgacagta cctttggtt aacgcagca 
      cgtggggat gaggagttg ggggattac gacccattt 
      gcccaccgc aaactcgac cacccaaca tctgacttg 
      gacactctc attcatttg ctgaaaggg agcttgggc 
      agtgggatt ctggcaatg cccatggct ttcgccaat 
      gctggtctc ttcttcgga ttgttttgc acgtttgca 
      atcggcagc atctgtacg tactgcgtg cacatattg 
      gtaaaaagc gctcatgcc ttgtgtaga aggaaccga 
      gttccgtct ctaggctac gcagaagtg gccgaagct 
      gcgttcctc gctggcccg caatcgatt cgtccatgg 
      gccaagttt gccaaagca atgatcaat ctgttcctt 
      gtaatagac ctcctgggc tgttgctgt gtgtacatc 
      atgtttgtt gccgagaat attaagcaa gttgttgac 
      aattacacg ccgaaggac actcatcaa gacattaag 
      atttacatg gccgccctc ttacccctc cttatccta 
      atcaatttg ataaggaat ctgaaatac ttgtcgcca 
      ctatctatg attgccaac atcctggtc gcctctgga 
      atgggcata acctactac tatctcatt aacgagatg 
      ccctctctt gacgagagg cccaaaatt gtcgaattc 
      acaaaacta ccaaagttc tttggtacc gttatcttt 
      gccttggaa ggcattggc gtggtaatg cctctcgaa 
      aacaacatg aagacacca actcacttt atcggatgt 
      cctggtgtt ttaaacatt ggaatgttt gtggtcgtt 
      cttttgtac gccacgacc ggtttcctg ggttatctc 
      aaatacggc acgcacaca caacccagc ataacgcta 
      aatcttcca gaaactgaa ccattggga cagtccgtg 
      aaactgatg atcgcagtt gcggttttc ttcacgtat 
      gctttgcag ttctatgtc cccatggag atcatctgg 
      aagaatttg aagggtttg ttcaacaag cgacccaac 
      ctctcagaa tacagcatt cgaattagt ttagttatt 
      ttgaccgcg gtcattgcc attctcgtt cctgatctt 
      gagggtctg atttcgcta gtcggagcg ttgtgtctt 
      tcaatgtta gggttgatc ttcccggct gttatcgat 
      ttagtcacc ttctgggaa gacccaggc tttgggagg 
      ctgaattgg cggctgtgg aagaacgtt ttgttgatt 
      ttcttcggg tttgtgggc tttgccacc gggacttat 
      gtcagcata caagggatc attgagaga gctaatagt 
      tcataa
    mRNA sequence
    • >TC032584 RA
      atacttagg tacagaaaa cttctttat ataatcgaa 
      ttgtatcaa gcagggagc tacttgcac gtgtgttag 
      tgtgtgttt tggttagaa aaaatgtct cgaaaatct 
      ggaacggta cctctacat gaggcatgc aagcctaaa 
      ctcatagct caagtagac cagaaaaag ggtggtgtt 
      aacacagta gtgatgact aattataaa tgtgaaaaa 
      aatgaagtg actgtgcct tcggccaat ggctcgaca 
      gtacctttg gttaacgca gcacgtggg gatgaggag 
      ttgggggat tacgaccca tttgcccac cgcaaactc 
      gaccaccca acatctgac ttggacact ctcattcat 
      ttgctgaaa gggagcttg ggcagtggg attctggca 
      atgcccatg gctttcgcc aatgctggt ctcttcttc 
      ggattgttt tgcacgttt gcaatcggc agcatctgt 
      acgtactgc gtgcacata ttggtaaaa agcgctcat 
      gccttgtgt agaaggaac cgagttccg tctctaggc 
      tacgcagaa gtggccgaa gctgcgttc ctcgctggc 
      ccgcaatcg attcgtcca tgggccaag tttgccaaa 
      gcaatgatc aatctgttc cttgtaata gacctcctg 
      ggctgttgc tgtgtgtac atcatgttt gttgccgag 
      aatattaag caagttgtt gacaattac acgccgaag 
      gacactcat caagacatt aagatttac atggccgcc 
      ctcttaccc ctccttatc ctaatcaat ttgataagg 
      aatctgaaa tacttgtcg ccactatct atgattgcc 
      aacatcctg gtcgcctct ggaatgggc ataacctac 
      tactatctc attaacgag atgccctct cttgacgag 
      aggcccaaa attgtcgaa ttcacaaaa ctaccaaag 
      ttctttggt accgttatc tttgccttg gaaggcatt 
      ggcgtggta atgcctctc gaaaacaac atgaagaca 
      ccaactcac tttatcgga tgtcctggt gttttaaac 
      attggaatg tttgtggtc gttcttttg tacgccacg 
      accggtttc ctgggttat ctcaaatac ggcacgcac 
      acacaaccc agcataacg ctaaatctt ccagaaact 
      gaaccattg ggacagtcc gtgaaactg atgatcgca 
      gttgcggtt ttcttcacg tatgctttg cagttctat 
      gtccccatg gagatcatc tggaagaat ttgaagggt 
      ttgttcaac aagcgaccc aacctctca gaatacagc 
      attcgaatt agtttagtt attttgacc gcggtcatt 
      gccattctc gttcctgat cttgagggt ctgatttcg 
      ctagtcgga gcgttgtgt ctttcaatg ttagggttg 
      atcttcccg gctgttatc gatttagtc accttctgg 
      gaagaccca ggctttggg aggctgaat tggcggctg 
      tggaagaac gttttgttg attttcttc gggtttgtg 
      ggctttgcc accgggact tatgtcagc atacaaggg 
      atcattgag agagctaat agttcataa tttcggcgg 
      ttttctatc gtctctagt gcacaggac cttgcccgt 
      taaattact gtctaaatc taaaacgta actagcctc 
      gagttggcc ttgcaaggt ttcaaagta gtgaccggc 
      tggaacccg gtagttaga caacaacga tttggattc 
      tggtttaga ttagttctg atccaagca ccaaagggg 
      acgtgtaga aaacgcttt atttttagt gtttaaaag 
      tagactgca agtacaatc tgtgattgc tataatctt 
      actatcttg tttcatgac ccttgcatt gattccttt 
      attttggtt taatttttt tgtatagtg agacattct 
      acattcatt cagttattt atatgtacc attgtgatt 
      tttttaggt ataagttac taacacttt aaactggaa 
      gttttgttg gtgttcatc aacacttgt tttgacttt 
      ccaagaacc aaaaatttt tacatgaac accggcaat 
      atgcagttt tctttaaca aacgcagta tttagttcc 
      aaaggaact gtgacttga tggtgcctt atgaaattt 
      ttatctggt tttttccag acgcaggaa cattgttga 
      taacgtaat ttgttggga aattgattt actttagat 
      ataatttta tgttcctta tatttatac acatagacg 
      aggaaaaat tgaaacgtt tcatattcg aaatttaag 
      taaattatt aatttcaag tcaaataaa tttatgtat 
      ttttctatc atcgttgcc aacactgtt aaaacagat 
      tcaagccgt actattgac gcaatttag caacagctt 
      aatacataa atttaagtg aaaactcga attaagtca 
      gaagttccc tattaattt taatatctt gatattgat 
      gagtaacat atttaaaat aaaatatgg tttttgat
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