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Gene information
General information
TC003871 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24028875..24029966
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003871
    iBeetle screen iB_07060

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003871 RA
      MSNKLTRYE KIEFLGEGQ FATVYKARD VETDNIVAV 
      KKIKMGSRQ EAQDGINRT ALREIKLLQ ELHHRNVIG 
      LLDVFGHMS NVSLVFDFM DTDLEVIIK DNTIILTTG 
      NIKAYIIQT LQGLDYLHR NWVLHRDLK PNNLLVNSN 
      GVLKIGDFG LAKLYGSPN RINTHQVVT RWYRCPELL 
      FGAKLYSTG VDMWAVGCI LAELLLRVP LFPGESDLD 
      QLTKIFAVF GNPTEENWP GLKSLSDYV EFKPFTPIP 
      LKNIFTAAG DDLLDVLEG LLVLNPLKR FECSKCLAM 
      PFFSNKPAP TPGSKLPLP QNIRKTEVE RPTLKRKLL 
      DAVEGGSLS KRLFF
    CDS sequence
    • >TC003871 RA
      atgtcgaat aaactaacc cgttacgaa aaaatcgag 
      tttctgggc gagggccaa ttcgccacg gtgtacaaa 
      gcccgcgat gtagagacc gacaacatc gtcgcagtg 
      aaaaaaatc aaaatgggg agtcgtcaa gaggcccaa 
      gacggcatc aaccgaaca gcccttcgc gaaatcaaa 
      ctcttacaa gaactgcac caccgcaac gtaatcggc 
      ctcttggac gtcttcgga cacatgtcc aacgtgtcc 
      cttgtgttt gatttcatg gacacggat ttggaagtt 
      ataataaaa gacaacacg attatttta acaactggc 
      aacataaaa gcctacatt attcaaacg ctgcaaggg 
      ctggactac ttgcaccga aactgggtc cttcaccgg 
      gacctcaag cccaacaac cttctggtg aactccaac 
      ggcgtgctc aaaatcggc gatttcggg cttgcgaag 
      ctctacggc tccccaaac cgcatcaac acgcaccag 
      gtggtcacc aggtggtac aggtgcccc gagctgctc 
      ttcggggca aagttgtac agcacgggg gtggacatg 
      tgggctgtg gggtgcatc ctcgcggag ctcctcctg 
      cgagtgccc ttgtttcct ggcgagtcg gacctggac 
      cagctgacc aaaattttc gcagttttt ggaaatccg 
      accgaggag aattggccg gggctgaag agtttgtcc 
      gattacgtc gaatttaag ccgtttacg ccgattccg 
      ttgaagaat attttcaca gccgccggt gatgatctg 
      cttgatgtc ttggagggg cttttggtg ttgaatccg 
      ttgaagcgg ttcgagtgc agcaagtgt ttggcgatg 
      ccgtttttc agtaataaa ccggcgccg acgccggga 
      tccaagttg ccgttgcct caaaacatc agaaaaact 
      gaagtggaa aggcctact ttgaagagg aagctgctg 
      gatgcggtg gagggaggg tcgctatct aaacgattg 
      tttttctaa
    mRNA sequence
    • >TC003871 RA
      ccctcataa cctcaaacc ggcaatttt caaaataat 
      gtgataaaa atgtcgaat aaactaacc cgttacgaa 
      aaaatcgag tttctgggc gagggccaa ttcgccacg 
      gtgtacaaa gcccgcgat gtagagacc gacaacatc 
      gtcgcagtg aaaaaaatc aaaatgggg agtcgtcaa 
      gaggcccaa gacggcatc aaccgaaca gcccttcgc 
      gaaatcaaa ctcttacaa gaactgcac caccgcaac 
      gtaatcggc ctcttggac gtcttcgga cacatgtcc 
      aacgtgtcc cttgtgttt gatttcatg gacacggat 
      ttggaagtt ataataaaa gacaacacg attatttta 
      acaactggc aacataaaa gcctacatt attcaaacg 
      ctgcaaggg ctggactac ttgcaccga aactgggtc 
      cttcaccgg gacctcaag cccaacaac cttctggtg 
      aactccaac ggcgtgctc aaaatcggc gatttcggg 
      cttgcgaag ctctacggc tccccaaac cgcatcaac 
      acgcaccag gtggtcacc aggtggtac aggtgcccc 
      gagctgctc ttcggggca aagttgtac agcacgggg 
      gtggacatg tgggctgtg gggtgcatc ctcgcggag 
      ctcctcctg cgagtgccc ttgtttcct ggcgagtcg 
      gacctggac cagctgacc aaaattttc gcagttttt 
      ggaaatccg accgaggag aattggccg gggctgaag 
      agtttgtcc gattacgtc gaatttaag ccgtttacg 
      ccgattccg ttgaagaat attttcaca gccgccggt 
      gatgatctg cttgatgtc ttggagggg cttttggtg 
      ttgaatccg ttgaagcgg ttcgagtgc agcaagtgt 
      ttggcgatg ccgtttttc agtaataaa ccggcgccg 
      acgccggga tccaagttg ccgttgcct caaaacatc 
      agaaaaact gaagtggaa aggcctact ttgaagagg 
      aagctgctg gatgcggtg gagggaggg tcgctatct 
      aaacgattg tttttctaa tttttttgt aaataaatt 
      attttttag taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY