Status
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Gene information
General information
TC034625 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23527402..23536070
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034625
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07057

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Decreased fluorescence included
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    vertex triplet seta partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    antenna partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna partially shape thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034625 RA
      MVTRVGPKK DPTLCLNAC RYLREYCET CNIHGVHYL 
      TERRPKAEK IFWFLTLLL SISGCSFMI HEIWIKYQK 
      NPIVVSFAN EVKSLYEIP FPAVTVCPE SKCSQSKFN 
      YSRVYGKLL NNESLSHFE SHAFFYLRM VCKNLGNEF 
      PNLLVNKTV GDDFFLLIE ELKPNIELS CSFLEHFTC 
      DESSYTPII TDVGICYSM NMLEKSEIF RPNVIYDEK 
      LNFTKSNYS SGFDGETGF TESAGVHSY PYRAFKSGV 
      DNSLDLVLV DQVDYDEKC SAVQGYRLL IHRPQDIPR 
      MSKNYIRVS HDKETTVAI EPALILTSP EVKNINPKK 
      RRCYLKGEK VLKYFLVYS QSNCQLECY TNFTMKTCG 
      CVFYFMPRE GETPVCGAG SYNCLELVK VQFKLEEVK 
      IKRSDPSEY ACGCRPSCN NLHYNVEVS QTDVSYQTF 
      SENVDRNAS EKRRYAQVK IYFKHDEFM SLERKELYV 
      FLQLELAVN CSVVESSSN SAMSSDNTN SDIIPTRNY 
      TSSGEYGNW WLLKRISKD SDRLQGDPW LLSKQEFRM 
      RKSWLSRSP KAAFRIQYG NSNEIGAFI RLTNSYCLM 
      GGEPQQYEK IQRIIQSDL GEIPAINTS LAGCKVVGR 
      LCVGNKNGL LVPELTYDT ELQRLKEEL PESLKISKI 
      EDKLSALGN VIVCNDSVA LIHPDLDRE TEEVIADTL 
      QVEVFRHMI SNNPLVGSH CTLNNLGGL VNSDIGQNE 
      LENLSSLLQ LSLVVTWNC KSREQTYWL WCSM
    CDS sequence
    • >TC034625 RA
      atggttacc cgtgtgggc cccaaaaag gaccccact 
      ctatgtctt aacgcttgc agatatttg cgtgaatat 
      tgtgaaaca tgtaacata catggcgtt cattattta 
      actgaaaga agaccaaaa gccgaaaag atattttgg 
      ttcctcaca ctgttatta tccataagt ggctgtagt 
      tttatgatt cacgaaatt tggatcaag taccaaaaa 
      aatccgatc gttgtcagt tttgcaaac gaagtcaag 
      tctttatac gaaatacct tttccggca gtcactgtc 
      tgtccggaa tcaaagtgt tcacaatcg aagtttaat 
      tacagccgt gtttatgga aagttgctc aataatgaa 
      tccctttca cactttgaa tctcatgct tttttttac 
      ttacgaatg gtttgtaag aatttggga aacgaattt 
      cccaactta ctagtgaat aaaacggtc ggtgacgat 
      ttttttcta cttatagag gagttaaag cccaacatc 
      gaactaagt tgctcgttc ttggaacat tttacctgt 
      gatgaaagt agttacact ccaatcatt acagatgta 
      ggaatctgc tattccatg aacatgttg gaaaaatcg 
      gaaattttc cgccccaat gtaatctat gacgaaaag 
      ttaaatttt accaagtcc aattattcc agtggattt 
      gatggtgaa actggattt acagaaagt gcaggagtg 
      cacagttat ccttacaga gctttcaaa tcaggtgtt 
      gataactca ttagactta gtgttagtt gatcaagta 
      gattatgac gaaaagtgt tcagctgtg caaggatat 
      cgtttgtta attcatagg cctcaagat attcctcga 
      atgtcgaaa aattatatc agagtgtca cacgataag 
      gaaacaacc gtcgctata gaacctgca ctcatttta 
      acatctccc gaggtgaaa aacattaat ccaaaaaaa 
      cgaaggtgt tacttgaaa ggtgaaaag gttttgaag 
      tattttttg gtctacagc cagtcaaat tgccaactg 
      gaatgttac acgaatttt accatgaaa acatgtgga 
      tgtgtgttc tattttatg cctagggag ggcgagact 
      cctgtttgt ggtgctgga agttataac tgcttagaa 
      ctcgttaaa gtgcaattc aaactcgaa gaagtgaaa 
      ataaagaga tcagatccg agcgaatat gcatgtggt 
      tgtaggccg tcttgtaat aatttacat tacaatgtg 
      gaagtgtct cagacagat gtttcatat caaacattt 
      tcagagaat gttgataga aatgccagt gaaaaacga 
      agatatgca caagtaaaa atttacttc aaacacgac 
      gaatttatg tcacttgaa cgaaaagaa ctctacgtt 
      tttttacaa ctcgaactg gctgtgaac tgtagtgta 
      gtggaatcc tcttcaaat tctgcaatg tccagtgat 
      aatacaaat agtgatatt attcctaca cgtaattac 
      accagcagt ggagaatat gggaactgg tggctatta 
      aaaagaata tcaaaagat tcggaccgt ttacagggc 
      gacccatgg ttactatca aaacaagaa ttcagaatg 
      cgtaaaagt tggctctcg agatcaccc aaagcagcg 
      tttcgaata caatacggg aatagtaat gaaattggt 
      gcttttata agattaaca aattcatat tgtctcatg 
      ggtggtgag ccccaacag tacgaaaaa atacaaaga 
      attattcaa agtgacctc ggcgagatt cctgcgatt 
      aacacttcc ttggctggg tgtaaagta gtaggacga 
      ttatgtgta ggcaataaa aatggactt ttggtgcct 
      gaactgacc tacgatacg gaattacaa cgactaaaa 
      gaagaactc cctgagtct ttaaaaata tcaaagatt 
      gaagataaa ttgtcggcc ttaggcaat gtgattgtt 
      tgcaatgat agtgtcgca cttattcac ccagatttg 
      gaccgagaa actgaagaa gtgatagca gatacctta 
      caagttgaa gtttttcgc catatgata tccaacaat 
      cccttagta ggttcgcat tgtacttta aataattta 
      ggaggatta gtgaattcg gatattggt caaaacgaa 
      ctcgaaaat ttatcgtcg ctgttacag ttatcctta 
      gttgtaacc tggaactgt aaatcaagg gaacaaact 
      tattggctc tggtgtagt atgtag
    mRNA sequence
    • >TC034625 RA
      gttccagaa tttgcttta ctaaattaa aggaactaa 
      agaatgaag taaaaatgg ttacccgtg tgggcccca 
      aaaaggacc ccactctat gtcttaacg cttgcagat 
      atttgcgtg aatattgtg aaacatgta acatacatg 
      gcgttcatt atttaactg aaagaagac caaaagccg 
      aaaagatat tttggttcc tcacactgt tattatcca 
      taagtggct gtagtttta tgattcacg aaatttgga 
      tcaagtacc aaaaaaatc cgatcgttg tcagttttg 
      caaacgaag tcaagtctt tatacgaaa taccttttc 
      cggcagtca ctgtctgtc cggaatcaa agtgttcac 
      aatcgaagt ttaattaca gccgtgttt atggaaagt 
      tgctcaata atgaatccc tttcacact ttgaatctc 
      atgcttttt tttacttac gaatggttt gtaagaatt 
      tgggaaacg aatttccca acttactag tgaataaaa 
      cggtcggtg acgattttt ttctactta tagaggagt 
      taaagccca acatcgaac taagttgct cgttcttgg 
      aacatttta cctgtgatg aaagtagtt acactccaa 
      tcattacag atgtaggaa tctgctatt ccatgaaca 
      tgttggaaa aatcggaaa ttttccgcc ccaatgtaa 
      tctatgacg aaaagttaa attttacca agtccaatt 
      attccagtg gatttgatg gtgaaactg gatttacag 
      aaagtgcag gagtgcaca gttatcctt acagagctt 
      tcaaatcag gtgttgata actcattag acttagtgt 
      tagttgatc aagtagatt atgacgaaa agtgttcag 
      ctgtgcaag gatatcgtt tgttaattc ataggcctc 
      aagatattc ctcgaatgt cgaaaaatt atatcagag 
      tgtcacacg ataaggaaa caaccgtcg ctatagaac 
      ctgcactca ttttaacat ctcccgagg tgaaaaaca 
      ttaatccaa aaaaacgaa ggtgttact tgaaaggtg 
      aaaaggttt tgaagtatt ttttggtct acagccagt 
      caaattgcc aactggaat gttacacga attttacca 
      tgaaaacat gtggatgtg tgttctatt ttatgccta 
      gggagggcg agactcctg tttgtggtg ctggaagtt 
      ataactgct tagaactcg ttaaagtgc aattcaaac 
      tcgaagaag tgaaaataa agagatcag atccgagcg 
      aatatgcat gtggttgta ggccgtctt gtaataatt 
      tacattaca atgtggaag tgtctcaga cagatgttt 
      catatcaaa cattttcag agaatgttg atagaaatg 
      ccagtgaaa aacgaagat atgcacaag taaaaattt 
      acttcaaac acgacgaat ttatgtcac ttgaacgaa 
      aagaactct acgtttttt tacaactcg aactggctg 
      tgaactgta gtgtagtgg aatcctctt caaattctg 
      caatgtcca gtgataata caaatagtg atattattc 
      ctacacgta attacacca gcagtggag aatatggga 
      actggtggc tattaaaaa gaatatcaa aagattcgg 
      accgtttac agggcgacc catggttac tatcaaaac 
      aagaattca gaatgcgta aaagttggc tctcgagat 
      cacccaaag cagcgtttc gaatacaat acgggaata 
      gtaatgaaa ttggtgctt ttataagat taacaaatt 
      catattgtc tcatgggtg gtgagcccc aacagtacg 
      aaaaaatac aaagaatta ttcaaagtg acctcggcg 
      agattcctg cgattaaca cttccttgg ctgggtgta 
      aagtagtag gacgattat gtgtaggca ataaaaatg 
      gacttttgg tgcctgaac tgacctacg atacggaat 
      tacaacgac taaaagaag aactccctg agtctttaa 
      aaatatcaa agattgaag ataaattgt cggccttag 
      gcaatgtga ttgtttgca atgatagtg tcgcactta 
      ttcacccag atttggacc gagaaactg aagaagtga 
      tagcagata ccttacaag ttgaagttt ttcgccata 
      tgatatcca acaatccct tagtaggtt cgcattgta 
      ctttaaata atttaggag gattagtga attcggata 
      ttggtcaaa acgaactcg aaaatttat cgtcgctgt 
      tacagttat ccttagttg taacctgga actgtaaat 
      caagggaac aaacttatt ggctctggt gtagtatgt 
      agtgacagt tttgcatat gctggcatg aaaactact 
      gctagagaa tttactgac attgagaat gcatttcta 
      ttaaaaata aattaaatt aaaccga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY