Status
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Gene information
General information
TC002587 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22640609..22768724
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002587
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07049

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002587 RA
      MAVPIACTR FSDNYDLKE ELGKGAFSV VRRCVQKST 
      GLEFAAKII NTKKLSARD FQKLEREAR ICRKLQHPN 
      IVRLHDSIQ EENFHYLVF DLVTGGELF EDIVAREFY 
      SEADASHCI QQILESVNH CHQNGVVHR DLKPENLLL 
      ASKAKGAAV KLADFGLAI EVQGEQQAW FGFAGTPGY 
      LSPEVLKKE PYGKPVDIW ACGVILYIL LVGYPPFWD 
      EDQHRLYAQ IKAGAYDYP SPEWDTVTP EAKNLINQM 
      LTVNPGKRI TASEALKHP WICQRERVA SVVHRQETV 
      DCLKKFNAR RKLKGAILT TMLATRNFS SRSIIVKKG 
      DGSQVKEST DSSTTLEDD DIKEDKKGG VDRSSTVIA 
      KDPEDLDSV HEIDTEHWV ESTVGYNRF PTEESARRQ 
      EIIKMTEQL IEAINTGDF EAYTKICDP HLTAFEPEA 
      MGNLVEGMD FHKFYFDNV LGKNCKAVN TTILNPHVH 
      LLGEDAACI AYVRLTQYM DKHGQAHTH QSEESRVWH 
      KRDNKWQNV HFHRSGGTG GAAFGFSSH K
    • >TC002587 RB
      MAVPIACTR FSDNYDLKE ELGKGAFSV VRRCVQKST 
      GLEFAAKII NTKKLSARD FQKLEREAR ICRKLQHPN 
      IVRLHDSIQ EENFHYLVF DLVTGGELF EDIVAREFY 
      SEADASHCI QQILESVNH CHQNGVVHR DLKPENLLL 
      ASKAKGAAV KLADFGLAI EVQGEQQAW FGFAGTPGY 
      LSPEVLKKE PYGKPVDIW ACGVILYIL LVGYPPFWD 
      EDQHRLYAQ IKAGAYDYP SPEWDTVTP EAKNLINQM 
      LTVNPGKRI TASEALKHP WICQRERVA SVVHRQETV 
      DCLKKFNAR RKLKGAILT TMLATRNFS SRSIIVKKG 
      DGSQVKEST DSSTTLEDD DIKEDKKGG VDRSSTVIA 
      KDPEGSGTP PQSPRIAPS SSSSPSSSV AAANPRPNA 
      LNLGQALLQ GVQDLDSVH EIDTEHWVE STVGYNRFP 
      TEESARRQE IIKMTEQLI EAINTGDFE AYTKICDPH 
      LTAFEPEAM GNLVEGMDF HKFYFDNVL GKNCKAVNT 
      TILNPHVHL LGEDAACIA YVRLTQYMD KHGQAHTHQ 
      SEESRVWHK RDNKWQNVH FHRSGGTGG AAFGFSSHK
    CDS sequence
    • >TC002587 RA
      atggcggtg cccattgcc tgtacgcgt tttagcgac 
      aactacgac cttaaagag gagcttgga aagggagct 
      ttttcagta gttagaaga tgtgttcag aaatcaaca 
      ggcttggaa ttcgccgcc aaaataata aatacaaaa 
      aaattatca gcgagagat ttccaaaag ttagaaaga 
      gaagcccga atttgtagg aaattacag catccaaac 
      atagtgagg ttacacgac agtatacaa gaagaaaat 
      tttcattat ttagtattt gatttggta acgggtggt 
      gaactattt gaagatatt gtagcgcga gagttttat 
      tcagaagca gatgcatca cactgcata caacagata 
      ttagaatca gtaaatcat tgtcaccaa aacggtgta 
      gttcataga gatctcaag cctgaaaac cttctttta 
      gcaagtaaa gccaaagga gcagctgtg aaattagcc 
      gattttggt ctagcgata gaagtgcaa ggggaacaa 
      caagcctgg tttggattc gcaggaacg ccaggatat 
      ctctctcca gaagtactg aagaaggag ccctatggc 
      aaacctgta gacatatgg gcgtgtggt gttatatta 
      tacattctt ttagtcggt tatcctccg ttttgggat 
      gaggaccag caccggtta tacgcacag atcaaagct 
      ggtgcttat gactatccc agtccggag tgggatacg 
      gtcactcct gaggcaaaa aacctcatc aatcaaatg 
      cttacggtt aatccagga aagagaatt actgcctcg 
      gaagcttta aaacaccca tggatttgc caaagagag 
      cgtgtggcg tccgtcgtt catcggcaa gagactgtg 
      gattgcctc aagaaattc aatgccagg cgtaaactt 
      aagggtgca atacttaca acaatgttg gctacgcgt 
      aatttttca agtagaagt ataatcgtg aaaaaaggc 
      gacggatct caggtgaaa gaatcaacc gacagttca 
      acaacacta gaagacgat gatatcaaa gaggataag 
      aagggcgga gtcgaccgc agttccact gtaattgcc 
      aaagatccg gaagatttg gattctgtt cacgaaatt 
      gatactgaa cactgggtt gaatcaaca gttggatac 
      aaccgcttt ccaaccgaa gaatcagca cgaaggcaa 
      gagatcatc aaaatgact gagcaactc atagaagcg 
      atcaacacg ggagatttc gaggcttac acaaaaata 
      tgtgatcca cacttgaca gcatttgaa ccggaagcc 
      atgggcaac ttggtcgaa ggcatggat ttccacaag 
      ttctatttc gacaatgtt ctgggcaaa aactgcaaa 
      gcagtgaac accacaatt ctgaaccct cacgtccac 
      ttattgggc gaagacgca gcttgcatc gcttacgtg 
      aggctaaca cagtacatg gacaaacac ggccaagcg 
      cacactcac cagtctgag gaaagtcgc gtgtggcat 
      aaaagagac aacaaatgg caaaatgtc cattttcac 
      cgcagtggc ggcacaggc ggtgctgct ttcggcttc 
      agttcgcat aaatag
    • >TC002587 RB
      atggcggtg cccattgcc tgtacgcgt tttagcgac 
      aactacgac cttaaagag gagcttgga aagggagct 
      ttttcagta gttagaaga tgtgttcag aaatcaaca 
      ggcttggaa ttcgccgcc aaaataata aatacaaaa 
      aaattatca gcgagagat ttccaaaag ttagaaaga 
      gaagcccga atttgtagg aaattacag catccaaac 
      atagtgagg ttacacgac agtatacaa gaagaaaat 
      tttcattat ttagtattt gatttggta acgggtggt 
      gaactattt gaagatatt gtagcgcga gagttttat 
      tcagaagca gatgcatca cactgcata caacagata 
      ttagaatca gtaaatcat tgtcaccaa aacggtgta 
      gttcataga gatctcaag cctgaaaac cttctttta 
      gcaagtaaa gccaaagga gcagctgtg aaattagcc 
      gattttggt ctagcgata gaagtgcaa ggggaacaa 
      caagcctgg tttggattc gcaggaacg ccaggatat 
      ctctctcca gaagtactg aagaaggag ccctatggc 
      aaacctgta gacatatgg gcgtgtggt gttatatta 
      tacattctt ttagtcggt tatcctccg ttttgggat 
      gaggaccag caccggtta tacgcacag atcaaagct 
      ggtgcttat gactatccc agtccggag tgggatacg 
      gtcactcct gaggcaaaa aacctcatc aatcaaatg 
      cttacggtt aatccagga aagagaatt actgcctcg 
      gaagcttta aaacaccca tggatttgc caaagagag 
      cgtgtggcg tccgtcgtt catcggcaa gagactgtg 
      gattgcctc aagaaattc aatgccagg cgtaaactt 
      aagggtgca atacttaca acaatgttg gctacgcgt 
      aatttttca agtagaagt ataatcgtg aaaaaaggc 
      gacggatct caggtgaaa gaatcaacc gacagttca 
      acaacacta gaagacgat gatatcaaa gaggataag 
      aagggcgga gtcgaccgc agttccact gtaattgcc 
      aaagatccg gaaggctct ggaactcct ccgcaatcg 
      ccaagaata gcaccttct tcttcaagt tcgccttct 
      agttctgtc gctgccgca aacccacga cccaatgct 
      cttaattta gggcaagct ttattgcaa ggggtacaa 
      gatttggat tctgttcac gaaattgat actgaacac 
      tgggttgaa tcaacagtt ggatacaac cgctttcca 
      accgaagaa tcagcacga aggcaagag atcatcaaa 
      atgactgag caactcata gaagcgatc aacacggga 
      gatttcgag gcttacaca aaaatatgt gatccacac 
      ttgacagca tttgaaccg gaagccatg ggcaacttg 
      gtcgaaggc atggatttc cacaagttc tatttcgac 
      aatgttctg ggcaaaaac tgcaaagca gtgaacacc 
      acaattctg aaccctcac gtccactta ttgggcgaa 
      gacgcagct tgcatcgct tacgtgagg ctaacacag 
      tacatggac aaacacggc caagcgcac actcaccag 
      tctgaggaa agtcgcgtg tggcataaa agagacaac 
      aaatggcaa aatgtccat tttcaccgc agtggcggc 
      acaggcggt gctgctttc ggcttcagt tcgcataaa 
      tag
    mRNA sequence
    • >TC002587 RA
      tagacgccg ccaccgccg ctgccgccg ccgccgcgc 
      cacaatatt gatctttta ggttatatc cccgaggag 
      cgacttcga gcacttcca gcagcatct agtggggat 
      tagacatcg tgtagtgtc cgcgagcgg ggccgacgc 
      gttcccaat ccatcagtg tcagcgtgc aaaaggtcc 
      cagcacaaa ttcctcaag tgcggtgtg tattttggg 
      tggcataag tcgcttcag ctgcgcgaa aaatcgctc 
      cgtactcgg tttattagt cggtcgagc gacactcag 
      cagtacgtt ttagggact gctgtcgtg agccatggc 
      ggtgcccat tgcctgtac gcgttttag cgacaacta 
      cgaccttaa agaggagct tggaaaggg agctttttc 
      agtagttag aagatgtgt tcagaaatc aacaggctt 
      ggaattcgc cgccaaaat aataaatac aaaaaaatt 
      atcagcgag agatttcca aaagttaga aagagaagc 
      ccgaatttg taggaaatt acagcatcc aaacatagt 
      gaggttaca cgacagtat acaagaaga aaattttca 
      ttatttagt atttgattt ggtaacggg tggtgaact 
      atttgaaga tattgtagc gcgagagtt ttattcaga 
      agcagatgc atcacactg catacaaca gatattaga 
      atcagtaaa tcattgtca ccaaaacgg tgtagttca 
      tagagatct caagcctga aaaccttct tttagcaag 
      taaagccaa aggagcagc tgtgaaatt agccgattt 
      tggtctagc gatagaagt gcaagggga acaacaagc 
      ctggtttgg attcgcagg aacgccagg atatctctc 
      tccagaagt actgaagaa ggagcccta tggcaaacc 
      tgtagacat atgggcgtg tggtgttat attatacat 
      tcttttagt cggttatcc tccgttttg ggatgagga 
      ccagcaccg gttatacgc acagatcaa agctggtgc 
      ttatgacta tcccagtcc ggagtggga tacggtcac 
      tcctgaggc aaaaaacct catcaatca aatgcttac 
      ggttaatcc aggaaagag aattactgc ctcggaagc 
      tttaaaaca cccatggat ttgccaaag agagcgtgt 
      ggcgtccgt cgttcatcg gcaagagac tgtggattg 
      cctcaagaa attcaatgc caggcgtaa acttaaggg 
      tgcaatact tacaacaat gttggctac gcgtaattt 
      ttcaagtag aagtataat cgtgaaaaa aggcgacgg 
      atctcaggt gaaagaatc aaccgacag ttcaacaac 
      actagaaga cgatgatat caaagagga taagaaggg 
      cggagtcga ccgcagttc cactgtaat tgccaaaga 
      tccggaaga tttggattc tgttcacga aattgatac 
      tgaacactg ggttgaatc aacagttgg atacaaccg 
      ctttccaac cgaagaatc agcacgaag gcaagagat 
      catcaaaat gactgagca actcataga agcgatcaa 
      cacgggaga tttcgaggc ttacacaaa aatatgtga 
      tccacactt gacagcatt tgaaccgga agccatggg 
      caacttggt cgaaggcat ggatttcca caagttcta 
      tttcgacaa tgttctggg caaaaactg caaagcagt 
      gaacaccac aattctgaa ccctcacgt ccacttatt 
      gggcgaaga cgcagcttg catcgctta cgtgaggct 
      aacacagta catggacaa acacggcca agcgcacac 
      tcaccagtc tgaggaaag tcgcgtgtg gcataaaag 
      agacaacaa atggcaaaa tgtccattt tcaccgcag 
      tggcggcac aggcggtgc tgctttcgg cttcagttc 
      gcataaata gaacagtac gccataact ttcatcgag 
      cattcatat ataaactat gtttactgt gaattctat 
      ttgtggtga tgtgtgatt ctgatattt ttgaatttt 
      atattgatt cttattgtt tttacatta ggtagcgta 
      gtttctcta tcacaatat tattgtgtt accaaccta 
      ttattccat ataatgtcc acactccgg gagacaaca 
      cttcagctt gcgttggtc accttgttg tttgactta 
      tatttccgt tgttttcgc gtagtctag taggtgtta 
      gtggatttt ttaagtcac aatcgtgtc tagtgagct 
      acattgtat ggaataaat gaatagatt gccttctac 
      gatatattt ctaggcttg ccattgaag ggttggtag 
      taatagcaa ccgtaactg aagcggcag ctttgcagc 
      acaccaaac ctaactata agatatatg aagtaacta 
      cttagccaa atgattata ggtaaaaac aaaaaaaag 
      agtatttat cggggtttt catttgtcc tttacacta 
      caatcagaa attgaaatt ctagggtag gattaatgg 
      tgcgaggat tattttata tttgtaaat tattgcttt 
      gtttccgcg attataaag tcttgacta ctgccttat 
      taaggttta agtaggggt gcagtactt atttattag 
      cgcactgat gcatggcat tttatagca ttcaagtat 
      cgaagttat aactcttat agagtacca cacacactt 
      ctacagggt gttccgtct taactccac attagaagt 
      attgggagt tgtaattgt gatagtcat ctgaaaatt 
      tgtatacag ctataatcc gtcaagtcc tcattaaaa 
      ctttcctat tttaaatgg aaagctgta ttttttact 
      gcatatttg aattactcg agttattgt aagggaatt 
      tttatcagc tttccctat acctaaatt tagtttttt 
      tcaaaatat ttacggttt ttgaaaatt tttggatta 
      gcatctttg acttcaaaa accgataac tttgccatt 
      tttttaaat tttgaaaat gtttttggc ttatttgaa 
      agggaaggt
    • >TC002587 RB
      tagacgccg ccaccgccg ctgccgccg ccgccgcgc 
      cacaatatt gatctttta ggttatatc cccgaggag 
      cgacttcga gcacttcca gcagcatct agtggggat 
      tagacatcg tgtagtgtc cgcgagcgg ggccgacgc 
      gttcccaat ccatcagtg tcagcgtgc aaaaggtcc 
      cagcacaaa ttcctcaag tgcggtgtg tattttggg 
      tggcataag tcgcttcag ctgcgcgaa aaatcgctc 
      cgtactcgg tttattagt cggtcgagc gacactcag 
      cagtacgtt ttagggact gctgtcgtg agccatggc 
      ggtgcccat tgcctgtac gcgttttag cgacaacta 
      cgaccttaa agaggagct tggaaaggg agctttttc 
      agtagttag aagatgtgt tcagaaatc aacaggctt 
      ggaattcgc cgccaaaat aataaatac aaaaaaatt 
      atcagcgag agatttcca aaagttaga aagagaagc 
      ccgaatttg taggaaatt acagcatcc aaacatagt 
      gaggttaca cgacagtat acaagaaga aaattttca 
      ttatttagt atttgattt ggtaacggg tggtgaact 
      atttgaaga tattgtagc gcgagagtt ttattcaga 
      agcagatgc atcacactg catacaaca gatattaga 
      atcagtaaa tcattgtca ccaaaacgg tgtagttca 
      tagagatct caagcctga aaaccttct tttagcaag 
      taaagccaa aggagcagc tgtgaaatt agccgattt 
      tggtctagc gatagaagt gcaagggga acaacaagc 
      ctggtttgg attcgcagg aacgccagg atatctctc 
      tccagaagt actgaagaa ggagcccta tggcaaacc 
      tgtagacat atgggcgtg tggtgttat attatacat 
      tcttttagt cggttatcc tccgttttg ggatgagga 
      ccagcaccg gttatacgc acagatcaa agctggtgc 
      ttatgacta tcccagtcc ggagtggga tacggtcac 
      tcctgaggc aaaaaacct catcaatca aatgcttac 
      ggttaatcc aggaaagag aattactgc ctcggaagc 
      tttaaaaca cccatggat ttgccaaag agagcgtgt 
      ggcgtccgt cgttcatcg gcaagagac tgtggattg 
      cctcaagaa attcaatgc caggcgtaa acttaaggg 
      tgcaatact tacaacaat gttggctac gcgtaattt 
      ttcaagtag aagtataat cgtgaaaaa aggcgacgg 
      atctcaggt gaaagaatc aaccgacag ttcaacaac 
      actagaaga cgatgatat caaagagga taagaaggg 
      cggagtcga ccgcagttc cactgtaat tgccaaaga 
      tccggaagg ctctggaac tcctccgca atcgccaag 
      aatagcacc ttcttcttc aagttcgcc ttctagttc 
      tgtcgctgc cgcaaaccc acgacccaa tgctcttaa 
      tttagggca agctttatt gcaaggggt acaagattt 
      ggattctgt tcacgaaat tgatactga acactgggt 
      tgaatcaac agttggata caaccgctt tccaaccga 
      agaatcagc acgaaggca agagatcat caaaatgac 
      tgagcaact catagaagc gatcaacac gggagattt 
      cgaggctta cacaaaaat atgtgatcc acacttgac 
      agcatttga accggaagc catgggcaa cttggtcga 
      aggcatgga tttccacaa gttctattt cgacaatgt 
      tctgggcaa aaactgcaa agcagtgaa caccacaat 
      tctgaaccc tcacgtcca cttattggg cgaagacgc 
      agcttgcat cgcttacgt gaggctaac acagtacat 
      ggacaaaca cggccaagc gcacactca ccagtctga 
      ggaaagtcg cgtgtggca taaaagaga caacaaatg 
      gcaaaatgt ccattttca ccgcagtgg cggcacagg 
      cggtgctgc tttcggctt cagttcgca taaatagaa 
      cagtacgcc ataactttc atcgagcat tcatatata 
      aactatgtt tactgtgaa ttctatttg tggtgatgt 
      gtgattctg atatttttg aattttata ttgattctt 
      attgttttt acattaggt agcgtagtt tctctatca 
      caatattat tgtgttacc aacctatta ttccatata 
      atgtccaca ctccgggag acaacactt cagcttgcg 
      ttggtcacc ttgttgttt gacttatat ttccgttgt 
      tttcgcgta gtctagtag gtgttagtg gatttttta 
      agtcacaat cgtgtctag tgagctaca ttgtatgga 
      ataaatgaa tagattgcc ttctacgat atatttcta 
      ggcttgcca ttgaagggt tggtagtaa tagcaaccg 
      taactgaag cggcagctt tgcagcaca ccaaaccta 
      actataaga tatatgaag taactactt agccaaatg 
      attataggt aaaaacaaa aaaaagagt atttatcgg 
      ggttttcat ttgtccttt acactacaa tcagaaatt 
      gaaattcta gggtaggat taatggtgc gaggattat 
      tttatattt gtaaattat tgctttgtt tccgcgatt 
      ataaagtct tgactactg ccttattaa ggtttaagt 
      aggggtgca gtacttatt tattagcgc actgatgca 
      tggcatttt atagcattc aagtatcga agttataac 
      tcttataga gtaccacac acacttcta cagggtgtt 
      ccgtcttaa ctccacatt agaagtatt gggagttgt 
      aattgtgat agtcatctg aaaatttgt atacagcta 
      taatccgtc aagtcctca ttaaaactt tcctatttt 
      aaatggaaa gctgtattt tttactgca tatttgaat 
      tactcgagt tattgtaag ggaattttt atcagcttt 
      ccctatacc taaatttag tttttttca aaatattta 
      cggtttttg aaaattttt ggattagca tctttgact 
      tcaaaaacc gataacttt gccattttt ttaaatttt 
      gaaaatgtt tttggctta tttgaaagg gaaggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY