| General information | |||
| TC002587 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 22640609..22768724 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002587 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07049 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002587 RA
MAVPIACTR FSDNYDLKE ELGKGAFSV VRRCVQKST GLEFAAKII NTKKLSARD FQKLEREAR ICRKLQHPN IVRLHDSIQ EENFHYLVF DLVTGGELF EDIVAREFY SEADASHCI QQILESVNH CHQNGVVHR DLKPENLLL ASKAKGAAV KLADFGLAI EVQGEQQAW FGFAGTPGY LSPEVLKKE PYGKPVDIW ACGVILYIL LVGYPPFWD EDQHRLYAQ IKAGAYDYP SPEWDTVTP EAKNLINQM LTVNPGKRI TASEALKHP WICQRERVA SVVHRQETV DCLKKFNAR RKLKGAILT TMLATRNFS SRSIIVKKG DGSQVKEST DSSTTLEDD DIKEDKKGG VDRSSTVIA KDPEDLDSV HEIDTEHWV ESTVGYNRF PTEESARRQ EIIKMTEQL IEAINTGDF EAYTKICDP HLTAFEPEA MGNLVEGMD FHKFYFDNV LGKNCKAVN TTILNPHVH LLGEDAACI AYVRLTQYM DKHGQAHTH QSEESRVWH KRDNKWQNV HFHRSGGTG GAAFGFSSH K
- >TC002587 RB
MAVPIACTR FSDNYDLKE ELGKGAFSV VRRCVQKST GLEFAAKII NTKKLSARD FQKLEREAR ICRKLQHPN IVRLHDSIQ EENFHYLVF DLVTGGELF EDIVAREFY SEADASHCI QQILESVNH CHQNGVVHR DLKPENLLL ASKAKGAAV KLADFGLAI EVQGEQQAW FGFAGTPGY LSPEVLKKE PYGKPVDIW ACGVILYIL LVGYPPFWD EDQHRLYAQ IKAGAYDYP SPEWDTVTP EAKNLINQM LTVNPGKRI TASEALKHP WICQRERVA SVVHRQETV DCLKKFNAR RKLKGAILT TMLATRNFS SRSIIVKKG DGSQVKEST DSSTTLEDD DIKEDKKGG VDRSSTVIA KDPEGSGTP PQSPRIAPS SSSSPSSSV AAANPRPNA LNLGQALLQ GVQDLDSVH EIDTEHWVE STVGYNRFP TEESARRQE IIKMTEQLI EAINTGDFE AYTKICDPH LTAFEPEAM GNLVEGMDF HKFYFDNVL GKNCKAVNT TILNPHVHL LGEDAACIA YVRLTQYMD KHGQAHTHQ SEESRVWHK RDNKWQNVH FHRSGGTGG AAFGFSSHK
- >TC002587 RA
atggcggtg cccattgcc tgtacgcgt tttagcgac aactacgac cttaaagag gagcttgga aagggagct ttttcagta gttagaaga tgtgttcag aaatcaaca ggcttggaa ttcgccgcc aaaataata aatacaaaa aaattatca gcgagagat ttccaaaag ttagaaaga gaagcccga atttgtagg aaattacag catccaaac atagtgagg ttacacgac agtatacaa gaagaaaat tttcattat ttagtattt gatttggta acgggtggt gaactattt gaagatatt gtagcgcga gagttttat tcagaagca gatgcatca cactgcata caacagata ttagaatca gtaaatcat tgtcaccaa aacggtgta gttcataga gatctcaag cctgaaaac cttctttta gcaagtaaa gccaaagga gcagctgtg aaattagcc gattttggt ctagcgata gaagtgcaa ggggaacaa caagcctgg tttggattc gcaggaacg ccaggatat ctctctcca gaagtactg aagaaggag ccctatggc aaacctgta gacatatgg gcgtgtggt gttatatta tacattctt ttagtcggt tatcctccg ttttgggat gaggaccag caccggtta tacgcacag atcaaagct ggtgcttat gactatccc agtccggag tgggatacg gtcactcct gaggcaaaa aacctcatc aatcaaatg cttacggtt aatccagga aagagaatt actgcctcg gaagcttta aaacaccca tggatttgc caaagagag cgtgtggcg tccgtcgtt catcggcaa gagactgtg gattgcctc aagaaattc aatgccagg cgtaaactt aagggtgca atacttaca acaatgttg gctacgcgt aatttttca agtagaagt ataatcgtg aaaaaaggc gacggatct caggtgaaa gaatcaacc gacagttca acaacacta gaagacgat gatatcaaa gaggataag aagggcgga gtcgaccgc agttccact gtaattgcc aaagatccg gaagatttg gattctgtt cacgaaatt gatactgaa cactgggtt gaatcaaca gttggatac aaccgcttt ccaaccgaa gaatcagca cgaaggcaa gagatcatc aaaatgact gagcaactc atagaagcg atcaacacg ggagatttc gaggcttac acaaaaata tgtgatcca cacttgaca gcatttgaa ccggaagcc atgggcaac ttggtcgaa ggcatggat ttccacaag ttctatttc gacaatgtt ctgggcaaa aactgcaaa gcagtgaac accacaatt ctgaaccct cacgtccac ttattgggc gaagacgca gcttgcatc gcttacgtg aggctaaca cagtacatg gacaaacac ggccaagcg cacactcac cagtctgag gaaagtcgc gtgtggcat aaaagagac aacaaatgg caaaatgtc cattttcac cgcagtggc ggcacaggc ggtgctgct ttcggcttc agttcgcat aaatag
- >TC002587 RB
atggcggtg cccattgcc tgtacgcgt tttagcgac aactacgac cttaaagag gagcttgga aagggagct ttttcagta gttagaaga tgtgttcag aaatcaaca ggcttggaa ttcgccgcc aaaataata aatacaaaa aaattatca gcgagagat ttccaaaag ttagaaaga gaagcccga atttgtagg aaattacag catccaaac atagtgagg ttacacgac agtatacaa gaagaaaat tttcattat ttagtattt gatttggta acgggtggt gaactattt gaagatatt gtagcgcga gagttttat tcagaagca gatgcatca cactgcata caacagata ttagaatca gtaaatcat tgtcaccaa aacggtgta gttcataga gatctcaag cctgaaaac cttctttta gcaagtaaa gccaaagga gcagctgtg aaattagcc gattttggt ctagcgata gaagtgcaa ggggaacaa caagcctgg tttggattc gcaggaacg ccaggatat ctctctcca gaagtactg aagaaggag ccctatggc aaacctgta gacatatgg gcgtgtggt gttatatta tacattctt ttagtcggt tatcctccg ttttgggat gaggaccag caccggtta tacgcacag atcaaagct ggtgcttat gactatccc agtccggag tgggatacg gtcactcct gaggcaaaa aacctcatc aatcaaatg cttacggtt aatccagga aagagaatt actgcctcg gaagcttta aaacaccca tggatttgc caaagagag cgtgtggcg tccgtcgtt catcggcaa gagactgtg gattgcctc aagaaattc aatgccagg cgtaaactt aagggtgca atacttaca acaatgttg gctacgcgt aatttttca agtagaagt ataatcgtg aaaaaaggc gacggatct caggtgaaa gaatcaacc gacagttca acaacacta gaagacgat gatatcaaa gaggataag aagggcgga gtcgaccgc agttccact gtaattgcc aaagatccg gaaggctct ggaactcct ccgcaatcg ccaagaata gcaccttct tcttcaagt tcgccttct agttctgtc gctgccgca aacccacga cccaatgct cttaattta gggcaagct ttattgcaa ggggtacaa gatttggat tctgttcac gaaattgat actgaacac tgggttgaa tcaacagtt ggatacaac cgctttcca accgaagaa tcagcacga aggcaagag atcatcaaa atgactgag caactcata gaagcgatc aacacggga gatttcgag gcttacaca aaaatatgt gatccacac ttgacagca tttgaaccg gaagccatg ggcaacttg gtcgaaggc atggatttc cacaagttc tatttcgac aatgttctg ggcaaaaac tgcaaagca gtgaacacc acaattctg aaccctcac gtccactta ttgggcgaa gacgcagct tgcatcgct tacgtgagg ctaacacag tacatggac aaacacggc caagcgcac actcaccag tctgaggaa agtcgcgtg tggcataaa agagacaac aaatggcaa aatgtccat tttcaccgc agtggcggc acaggcggt gctgctttc ggcttcagt tcgcataaa tag
- >TC002587 RA
tagacgccg ccaccgccg ctgccgccg ccgccgcgc cacaatatt gatctttta ggttatatc cccgaggag cgacttcga gcacttcca gcagcatct agtggggat tagacatcg tgtagtgtc cgcgagcgg ggccgacgc gttcccaat ccatcagtg tcagcgtgc aaaaggtcc cagcacaaa ttcctcaag tgcggtgtg tattttggg tggcataag tcgcttcag ctgcgcgaa aaatcgctc cgtactcgg tttattagt cggtcgagc gacactcag cagtacgtt ttagggact gctgtcgtg agccatggc ggtgcccat tgcctgtac gcgttttag cgacaacta cgaccttaa agaggagct tggaaaggg agctttttc agtagttag aagatgtgt tcagaaatc aacaggctt ggaattcgc cgccaaaat aataaatac aaaaaaatt atcagcgag agatttcca aaagttaga aagagaagc ccgaatttg taggaaatt acagcatcc aaacatagt gaggttaca cgacagtat acaagaaga aaattttca ttatttagt atttgattt ggtaacggg tggtgaact atttgaaga tattgtagc gcgagagtt ttattcaga agcagatgc atcacactg catacaaca gatattaga atcagtaaa tcattgtca ccaaaacgg tgtagttca tagagatct caagcctga aaaccttct tttagcaag taaagccaa aggagcagc tgtgaaatt agccgattt tggtctagc gatagaagt gcaagggga acaacaagc ctggtttgg attcgcagg aacgccagg atatctctc tccagaagt actgaagaa ggagcccta tggcaaacc tgtagacat atgggcgtg tggtgttat attatacat tcttttagt cggttatcc tccgttttg ggatgagga ccagcaccg gttatacgc acagatcaa agctggtgc ttatgacta tcccagtcc ggagtggga tacggtcac tcctgaggc aaaaaacct catcaatca aatgcttac ggttaatcc aggaaagag aattactgc ctcggaagc tttaaaaca cccatggat ttgccaaag agagcgtgt ggcgtccgt cgttcatcg gcaagagac tgtggattg cctcaagaa attcaatgc caggcgtaa acttaaggg tgcaatact tacaacaat gttggctac gcgtaattt ttcaagtag aagtataat cgtgaaaaa aggcgacgg atctcaggt gaaagaatc aaccgacag ttcaacaac actagaaga cgatgatat caaagagga taagaaggg cggagtcga ccgcagttc cactgtaat tgccaaaga tccggaaga tttggattc tgttcacga aattgatac tgaacactg ggttgaatc aacagttgg atacaaccg ctttccaac cgaagaatc agcacgaag gcaagagat catcaaaat gactgagca actcataga agcgatcaa cacgggaga tttcgaggc ttacacaaa aatatgtga tccacactt gacagcatt tgaaccgga agccatggg caacttggt cgaaggcat ggatttcca caagttcta tttcgacaa tgttctggg caaaaactg caaagcagt gaacaccac aattctgaa ccctcacgt ccacttatt gggcgaaga cgcagcttg catcgctta cgtgaggct aacacagta catggacaa acacggcca agcgcacac tcaccagtc tgaggaaag tcgcgtgtg gcataaaag agacaacaa atggcaaaa tgtccattt tcaccgcag tggcggcac aggcggtgc tgctttcgg cttcagttc gcataaata gaacagtac gccataact ttcatcgag cattcatat ataaactat gtttactgt gaattctat ttgtggtga tgtgtgatt ctgatattt ttgaatttt atattgatt cttattgtt tttacatta ggtagcgta gtttctcta tcacaatat tattgtgtt accaaccta ttattccat ataatgtcc acactccgg gagacaaca cttcagctt gcgttggtc accttgttg tttgactta tatttccgt tgttttcgc gtagtctag taggtgtta gtggatttt ttaagtcac aatcgtgtc tagtgagct acattgtat ggaataaat gaatagatt gccttctac gatatattt ctaggcttg ccattgaag ggttggtag taatagcaa ccgtaactg aagcggcag ctttgcagc acaccaaac ctaactata agatatatg aagtaacta cttagccaa atgattata ggtaaaaac aaaaaaaag agtatttat cggggtttt catttgtcc tttacacta caatcagaa attgaaatt ctagggtag gattaatgg tgcgaggat tattttata tttgtaaat tattgcttt gtttccgcg attataaag tcttgacta ctgccttat taaggttta agtaggggt gcagtactt atttattag cgcactgat gcatggcat tttatagca ttcaagtat cgaagttat aactcttat agagtacca cacacactt ctacagggt gttccgtct taactccac attagaagt attgggagt tgtaattgt gatagtcat ctgaaaatt tgtatacag ctataatcc gtcaagtcc tcattaaaa ctttcctat tttaaatgg aaagctgta ttttttact gcatatttg aattactcg agttattgt aagggaatt tttatcagc tttccctat acctaaatt tagtttttt tcaaaatat ttacggttt ttgaaaatt tttggatta gcatctttg acttcaaaa accgataac tttgccatt tttttaaat tttgaaaat gtttttggc ttatttgaa agggaaggt
- >TC002587 RB
tagacgccg ccaccgccg ctgccgccg ccgccgcgc cacaatatt gatctttta ggttatatc cccgaggag cgacttcga gcacttcca gcagcatct agtggggat tagacatcg tgtagtgtc cgcgagcgg ggccgacgc gttcccaat ccatcagtg tcagcgtgc aaaaggtcc cagcacaaa ttcctcaag tgcggtgtg tattttggg tggcataag tcgcttcag ctgcgcgaa aaatcgctc cgtactcgg tttattagt cggtcgagc gacactcag cagtacgtt ttagggact gctgtcgtg agccatggc ggtgcccat tgcctgtac gcgttttag cgacaacta cgaccttaa agaggagct tggaaaggg agctttttc agtagttag aagatgtgt tcagaaatc aacaggctt ggaattcgc cgccaaaat aataaatac aaaaaaatt atcagcgag agatttcca aaagttaga aagagaagc ccgaatttg taggaaatt acagcatcc aaacatagt gaggttaca cgacagtat acaagaaga aaattttca ttatttagt atttgattt ggtaacggg tggtgaact atttgaaga tattgtagc gcgagagtt ttattcaga agcagatgc atcacactg catacaaca gatattaga atcagtaaa tcattgtca ccaaaacgg tgtagttca tagagatct caagcctga aaaccttct tttagcaag taaagccaa aggagcagc tgtgaaatt agccgattt tggtctagc gatagaagt gcaagggga acaacaagc ctggtttgg attcgcagg aacgccagg atatctctc tccagaagt actgaagaa ggagcccta tggcaaacc tgtagacat atgggcgtg tggtgttat attatacat tcttttagt cggttatcc tccgttttg ggatgagga ccagcaccg gttatacgc acagatcaa agctggtgc ttatgacta tcccagtcc ggagtggga tacggtcac tcctgaggc aaaaaacct catcaatca aatgcttac ggttaatcc aggaaagag aattactgc ctcggaagc tttaaaaca cccatggat ttgccaaag agagcgtgt ggcgtccgt cgttcatcg gcaagagac tgtggattg cctcaagaa attcaatgc caggcgtaa acttaaggg tgcaatact tacaacaat gttggctac gcgtaattt ttcaagtag aagtataat cgtgaaaaa aggcgacgg atctcaggt gaaagaatc aaccgacag ttcaacaac actagaaga cgatgatat caaagagga taagaaggg cggagtcga ccgcagttc cactgtaat tgccaaaga tccggaagg ctctggaac tcctccgca atcgccaag aatagcacc ttcttcttc aagttcgcc ttctagttc tgtcgctgc cgcaaaccc acgacccaa tgctcttaa tttagggca agctttatt gcaaggggt acaagattt ggattctgt tcacgaaat tgatactga acactgggt tgaatcaac agttggata caaccgctt tccaaccga agaatcagc acgaaggca agagatcat caaaatgac tgagcaact catagaagc gatcaacac gggagattt cgaggctta cacaaaaat atgtgatcc acacttgac agcatttga accggaagc catgggcaa cttggtcga aggcatgga tttccacaa gttctattt cgacaatgt tctgggcaa aaactgcaa agcagtgaa caccacaat tctgaaccc tcacgtcca cttattggg cgaagacgc agcttgcat cgcttacgt gaggctaac acagtacat ggacaaaca cggccaagc gcacactca ccagtctga ggaaagtcg cgtgtggca taaaagaga caacaaatg gcaaaatgt ccattttca ccgcagtgg cggcacagg cggtgctgc tttcggctt cagttcgca taaatagaa cagtacgcc ataactttc atcgagcat tcatatata aactatgtt tactgtgaa ttctatttg tggtgatgt gtgattctg atatttttg aattttata ttgattctt attgttttt acattaggt agcgtagtt tctctatca caatattat tgtgttacc aacctatta ttccatata atgtccaca ctccgggag acaacactt cagcttgcg ttggtcacc ttgttgttt gacttatat ttccgttgt tttcgcgta gtctagtag gtgttagtg gatttttta agtcacaat cgtgtctag tgagctaca ttgtatgga ataaatgaa tagattgcc ttctacgat atatttcta ggcttgcca ttgaagggt tggtagtaa tagcaaccg taactgaag cggcagctt tgcagcaca ccaaaccta actataaga tatatgaag taactactt agccaaatg attataggt aaaaacaaa aaaaagagt atttatcgg ggttttcat ttgtccttt acactacaa tcagaaatt gaaattcta gggtaggat taatggtgc gaggattat tttatattt gtaaattat tgctttgtt tccgcgatt ataaagtct tgactactg ccttattaa ggtttaagt aggggtgca gtacttatt tattagcgc actgatgca tggcatttt atagcattc aagtatcga agttataac tcttataga gtaccacac acacttcta cagggtgtt ccgtcttaa ctccacatt agaagtatt gggagttgt aattgtgat agtcatctg aaaatttgt atacagcta taatccgtc aagtcctca ttaaaactt tcctatttt aaatggaaa gctgtattt tttactgca tatttgaat tactcgagt tattgtaag ggaattttt atcagcttt ccctatacc taaatttag tttttttca aaatattta cggtttttg aaaattttt ggattagca tctttgact tcaaaaacc gataacttt gccattttt ttaaatttt gaaaatgtt tttggctta tttgaaagg gaaggt
