General information | |||
TC032572
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 22385361..22484403 | |
Sequence information | |||
Transcripts / Proteins (4) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC032572 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00408 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
remarks: later than schedule, hours 24
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07047 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032572 RA
MATTVAMNE SNHVVYNRT ESTESGWEN PFRPGGDLS READEIVEL IKGGKPITP TPDSSAPPL PSLDETDTQ KNQTANNLS EPITQSPKK ASELNATPK SANGNVKND KPNAPGAVD VKRGTIKPD GDVEHVTIK KKPKCKCCV IQ
- >TC032572 RB
MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ EEPEPSPIP ENANPFPTD QDQTETERE KVRVTFFQQ SKPQEVDLP DEPVVFTDA DFIMATTVA MNESNHVVY NRTESTESG WENPFRPGG DLSREADEI VELIKGGKP ITPTPDSSA PPLPSLDET DTQKNQTAN NLSEPITQS PKKASELNA TPKSANGNV KNDKPNAPG AVDVKRGTI KPDGDVEHV TIKKKPKCK CCVIQ
- >TC032572 RC
MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ EEPEPSPIP ENANPFPTD QDQTEASSK FGNWWFAST WRRFSKVSQ CKCFCGRAS SAYQSQSIG VDKEPNEKK CE
- >TC032572 RD
MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ EEPEPSPIP ENANPFPTD QDQTE
- >TC032572 RA
atggccacc acggtagct atgaatgaa tcgaatcat gtcgtctac aataggacg gaaagtacg gaaagcggc tgggagaac cccttccga cctggcggc gacttgagt cgagaggcc gacgagata gtagaatta ataaaaggg ggcaaaccg ataacgcct acacccgac agttcggcg cccccttta ccctcctta gacgaaacc gatacgcaa aagaaccaa acagccaac aatctatcg gaacccata acacaaagc ccgaagaaa gccagcgaa ctgaatgcc accccgaaa agtgctaac ggcaacgtc aaaaacgac aaacccaac gctccagga gccgtcgac gttaaacga gggacgatt aaaccagac ggagacgtc gaacacgtt acgataaaa aagaaaccc aagtgcaag tgctgtgtt atacaataa
- >TC032572 RB
atgttgtcg tacatgcta ccagtggac gaaaaaccg ccaccggct cctaacaga ggacacatg cagttacct ttggcttct gaaactcta acaaacggt agaacttca agtcctatc agtaaagaa acaagtgtt agcagtttt gttagcgat gtgacaaca gcaacagct agtcacagc tctatgacg cctacaagc accagtgat aaaggaaag cctaagtta gttatcaat ggaggaaag caccaaaca ctgaaaagg gtatcgttt ggtagctcc aaggggtcc atggttgaa acgcttatt tttgaaagt cctgtccaa gaagaaccc gagccgagt ccgattcct gaaaatgca aaccctttt cctacagat caagatcaa actgaaacc gaacgagaa aaagtgcga gtgacgttc tttcaacaa tcaaaacct caagaagtc gatttaccg gatgaacct gtcgttttt accgacgct gatttcata atggccacc acggtagct atgaatgaa tcgaatcat gtcgtctac aataggacg gaaagtacg gaaagcggc tgggagaac cccttccga cctggcggc gacttgagt cgagaggcc gacgagata gtagaatta ataaaaggg ggcaaaccg ataacgcct acacccgac agttcggcg cccccttta ccctcctta gacgaaacc gatacgcaa aagaaccaa acagccaac aatctatcg gaacccata acacaaagc ccgaagaaa gccagcgaa ctgaatgcc accccgaaa agtgctaac ggcaacgtc aaaaacgac aaacccaac gctccagga gccgtcgac gttaaacga gggacgatt aaaccagac ggagacgtc gaacacgtt acgataaaa aagaaaccc aagtgcaag tgctgtgtt atacaataa
- >TC032572 RC
atgttgtcg tacatgcta ccagtggac gaaaaaccg ccaccggct cctaacaga ggacacatg cagttacct ttggcttct gaaactcta acaaacggt agaacttca agtcctatc agtaaagaa acaagtgtt agcagtttt gttagcgat gtgacaaca gcaacagct agtcacagc tctatgacg cctacaagc accagtgat aaaggaaag cctaagtta gttatcaat ggaggaaag caccaaaca ctgaaaagg gtatcgttt ggtagctcc aaggggtcc atggttgaa acgcttatt tttgaaagt cctgtccaa gaagaaccc gagccgagt ccgattcct gaaaatgca aaccctttt cctacagat caagatcaa actgaagca tcatcgaag ttcggaaat tggtggttt gcgagtact tggcggcgg ttttccaaa gtcagtcag tgcaagtgc ttttgtggt cgcgcgtcc tctgcctat caaagccaa agcatcggc gtcgataaa gaaccgaac gagaaaaag tgcgagtga
- >TC032572 RD
atgttgtcg tacatgcta ccagtggac gaaaaaccg ccaccggct cctaacaga ggacacatg cagttacct ttggcttct gaaactcta acaaacggt agaacttca agtcctatc agtaaagaa acaagtgtt agcagtttt gttagcgat gtgacaaca gcaacagct agtcacagc tctatgacg cctacaagc accagtgat aaaggaaag cctaagtta gttatcaat ggaggaaag caccaaaca ctgaaaagg gtatcgttt ggtagctcc aaggggtcc atggttgaa acgcttatt tttgaaagt cctgtccaa gaagaaccc gagccgagt ccgattcct gaaaatgca aaccctttt cctacagat caagatcaa actgagtaa
- >TC032572 RA
cacccactt gtcggattg tatggtggc gctctgttg caaaacagc gaactatcg ccaatccgc gatgtttga ggcgtggcg ttcgaagcg tgttgcgta cgagtgtta aatatttcg atgtaataa ttttataac ctcgtagag catcatcga agttcggaa attggtggt ttgcgagta cttggcggc ggttttcca aagtcagtc agtgcaagt gcttttgtg gtcgcgcgt cctctgcct atcaaagcc aaagcatcg gcgtcgata aagaaccga acgagaaaa agtgcgagt gacgttctt tcaacaatc aaaacctca agaagtcga tttaccgga tgaacctgt cgtttttac cgacgctga tttcataat ggccaccac ggtagctat gaatgaatc gaatcatgt cgtctacaa taggacgga aagtacgga aagcggctg ggagaaccc cttccgacc tggcggcga cttgagtcg agaggccga cgagatagt agaattaat aaaaggggg caaaccgat aacgcctac acccgacag ttcggcgcc ccctttacc ctccttaga cgaaaccga tacgcaaaa gaaccaaac agccaacaa tctatcgga acccataac acaaagccc gaagaaagc cagcgaact gaatgccac cccgaaaag tgctaacgg caacgtcaa aaacgacaa acccaacgc tccaggagc cgtcgacgt taaacgagg gacgattaa accagacgg agacgtcga acacgttac gataaaaaa gaaacccaa gtgcaagtg ctgtgttat acaataact gctgacaga tagtcaata gacttgtta ctgttaaat attttataa aaaataata gcaaggtgt gagtaaaaa cgattcggt tttgcgaga tggtggtgc taactgtga tgtttttag tacaaaaaa agcatctga tggccgatc gaccaattc gataaaatc ttccacaca aagcagttt tcaacctaa cgaaagtaa aaaattgca aacaaaaca tatcaatcc agtaataat tttgttgca attaaaaca gtcgatcag tggatcaga taatcgccc agaagtaca cgaaataat caaaaactg caaccgatt tcaggattt tcgttgaga agaaacgaa accgaaggt ttttacgcc acccagagc tgagaaatg cttacctat gctttaacc agtcacaca aatactagt ggtgcatgt tcaatctct acttaattt tcaagcaat actgaatat ttagttgta tggccaata ggaattgtc cgccttgtc cgtgacaga aagttttaa attcgactc tagtcaaaa ttatttttg tgattgtaa acggacaag gtggaacag tgtggttat tggcgctgt tattctact aaacgaatg tgattatat acagttata aaatttacc atattttta cattgtatt agagttgtt tgtgtttcg cgcttattt cttcacggt tgtgggagt tttctacag atgagataa acaaacacg gaatatggg aacacaaac agcggttta tatttagtt taacatttg tataaatat acaatgagt ctagaatgg atgatagta caagtattt tttatatat tttagcctt tcatcaaca tttttacta ctgtatgac agaaatttc ggattaacg gtgtattca gtagcgaga tttagttgt ctatgtttt actatttaa cttgtaaat gttctataa ataggtgcg acgttctag ttattgttg ttaatatta tttgttgtg tttgtgtgt ctggaatga aagtcggaa acatcggca ttatttttg gtaaaaaat aatacttga taaagataa ttaaatcgt accggaacg atcagacgg ttggtaata agtttcatt agtgaaatt tatttgnnn nnnnnnnnn nnnnnnnnn nnnaacacg aattgtgca gagattatt taatatttt aaatataag tacataacg tccctgttt atatgtgta tgtaaagat tgcagctta taatatgct taagatgta tagatttta gattgtaaa tcgatcatg tttgtttga agcactcca aactctttt ttaatgata ttttacatg aggaaattt ttattaata tattatttt aatcttttg tgtattata ttgtaatat aaatctaat ttgtatacc aagccgcat agcattaaa taatttgtg atgtc
- >TC032572 RB
tacaaagca gaagtagag tttcgatgg tccgctgtg tacaaatcg cgagctgcg ccgtgagta cgtcgcgga taaacaccg gctcctcca gctaaaatc gcgctaaga ctgcatagc tcctccatc gaattcgaa cggtggtgc agtgtaggt gtggtgtcg tggtcaagg gtaagccga cacttgccc cagtttcga gcccagatc ggtgcttct tcgattgtg accgtgaaa gtgtattag tgtaaaatg ttaaaatta agttagtgc ttcgtaatg ttgtcgtac atgctacca gtggacgaa aaaccgcca ccggctcct aacagagga cacatgcag ttacctttg gcttctgaa actctaaca aacggtaga acttcaagt cctatcagt aaagaaaca agtgttagc agttttgtt agcgatgtg acaacagca acagctagt cacagctct atgacgcct acaagcacc agtgataaa ggaaagcct aagttagtt atcaatgga ggaaagcac caaacactg aaaagggta tcgtttggt agctccaag gggtccatg gttgaaacg cttattttt gaaagtcct gtccaagaa gaacccgag ccgagtccg attcctgaa aatgcaaac ccttttcct acagatcaa gatcaaact gaaaccgaa cgagaaaaa gtgcgagtg acgttcttt caacaatca aaacctcaa gaagtcgat ttaccggat gaacctgtc gtttttacc gacgctgat ttcataatg gccaccacg gtagctatg aatgaatcg aatcatgtc gtctacaat aggacggaa agtacggaa agcggctgg gagaacccc ttccgacct ggcggcgac ttgagtcga gaggccgac gagatagta gaattaata aaagggggc aaaccgata acgcctaca cccgacagt tcggcgccc cctttaccc tccttagac gaaaccgat acgcaaaag aaccaaaca gccaacaat ctatcggaa cccataaca caaagcccg aagaaagcc agcgaactg aatgccacc ccgaaaagt gctaacggc aacgtcaaa aacgacaaa cccaacgct ccaggagcc gtcgacgtt aaacgaggg acgattaaa ccagacgga gacgtcgaa cacgttacg ataaaaaag aaacccaag tgcaagtgc tgtgttata caataactg ctgacagat agtcaatag acttgttac tgttaaata ttttataaa aaataatag caaggtgtg agtaaaaac gattcggtt ttgcgagat ggtggtgct aactgtgat gtttttagt acaaaaaaa gcatctgat ggccgatcg accaattcg ataaaatct tccacacaa agcagtttt caacctaac gaaagtaaa aaattgcaa acaaaacat atcaatcca gtaataatt ttgttgcaa ttaaaacag tcgatcagt ggatcagat aatcgccca gaagtacac gaaataatc aaaaactgc aaccgattt caggatttt cgttgagaa gaaacgaaa ccgaaggtt tttacgcca cccagagct gagaaatgc ttacctatg ctttaacca gtcacacaa atactagtg gtgcatgtt caatctcta cttaatttt caagcaata ctgaatatt tagttgtat ggccaatag gaattgtcc gccttgtcc gtgacagaa agttttaaa ttcgactct agtcaaaat tatttttgt gattgtaaa cggacaagg tggaacagt gtggttatt ggcgctgtt attctacta aacgaatgt gattatata cagttataa aatttacca tatttttac attgtatta gagttgttt gtgtttcgc gcttatttc ttcacggtt gtgggagtt ttctacaga tgagataaa caaacacgg aatatggga acacaaaca gcggtttat atttagttt aacatttgt ataaatata caatgagtc tagaatgga tgatagtac aagtatttt ttatatatt ttagccttt catcaacat ttttactac tgtatgaca gaaatttcg gattaacgg tgtattcag tagcgagat ttagttgtc tatgtttta ctatttaac ttgtaaatg ttctataaa taggtgcga cgttctagt tattgttgt taatattat ttgttgtgt ttgtgtgtc tggaatgaa agtcggaaa catcggcat tatttttgg taaaaaata atacttgat aaagataat taaatcgta ccggaacga tcagacggt tggtaataa gtttcatta gtgaaattt atttgnnnn nnnnnnnnn nnnnnnnnn nnaacacga attgtgcag agattattt aatatttta aatataagt acataacgt ccctgttta tatgtgtat gtaaagatt gcagcttat aatatgctt aagatgtat agattttag attgtaaat cgatcatgt ttgtttgaa gcactccaa actcttttt taatgatat tttacatga ggaaatttt tattaatat attatttta atcttttgt gtattatat tgtaatata aatctaatt tgtatacca agccgcata gcattaaat aatttgtga tgtc
- >TC032572 RC
tacaaagca gaagtagag tttcgatgg tccgctgtg tacaaatcg cgagctgcg ccgtgagta cgtcgcgga taaacaccg gctcctcca gctaaaatc gcgctaaga ctgcatagc tcctccatc gaattcgaa cggtggtgc agtgtaggt gtggtgtcg tggtcaagg gtaagccga cacttgccc cagtttcga gcccagatc ggtgcttct tcgattgtg accgtgaaa gtgtattag tgtaaaatg ttaaaatta agttagtgc ttcgtaatg ttgtcgtac atgctacca gtggacgaa aaaccgcca ccggctcct aacagagga cacatgcag ttacctttg gcttctgaa actctaaca aacggtaga acttcaagt cctatcagt aaagaaaca agtgttagc agttttgtt agcgatgtg acaacagca acagctagt cacagctct atgacgcct acaagcacc agtgataaa ggaaagcct aagttagtt atcaatgga ggaaagcac caaacactg aaaagggta tcgtttggt agctccaag gggtccatg gttgaaacg cttattttt gaaagtcct gtccaagaa gaacccgag ccgagtccg attcctgaa aatgcaaac ccttttcct acagatcaa gatcaaact gaagcatca tcgaagttc ggaaattgg tggtttgcg agtacttgg cggcggttt tccaaagtc agtcagtgc aagtgcttt tgtggtcgc gcgtcctct gcctatcaa agccaaagc atcggcgtc gataaagaa ccgaacgag aaaaagtgc gagtgacgt tctttcaac aatcaaaac ctcaagaag tcgatttac cggatgaac ctgtcgttt ttaccgacg ctgatttca taatggcca ccacggtag ctatgaatg aatcgaatc atgtcgtct acaatagga cggaaaggt gagtttttg tattgctgt aagtgacta tgtcacact aaaagctca caaagact
- >TC032572 RD
tacaaagca gaagtagag tttcgatgg tccgctgtg tacaaatcg cgagctgcg ccgtgagta cgtcgcgga taaacaccg gctcctcca gctaaaatc gcgctaaga ctgcatagc tcctccatc gaattcgaa cggtggtgc agtgtaggt gtggtgtcg tggtcaagg gtaagccga cacttgccc cagtttcga gcccagatc ggtgcttct tcgattgtg accgtgaaa gtgtattag tgtaaaatg ttaaaatta agttagtgc ttcgtaatg ttgtcgtac atgctacca gtggacgaa aaaccgcca ccggctcct aacagagga cacatgcag ttacctttg gcttctgaa actctaaca aacggtaga acttcaagt cctatcagt aaagaaaca agtgttagc agttttgtt agcgatgtg acaacagca acagctagt cacagctct atgacgcct acaagcacc agtgataaa ggaaagcct aagttagtt atcaatgga ggaaagcac caaacactg aaaagggta tcgtttggt agctccaag gggtccatg gttgaaacg cttattttt gaaagtcct gtccaagaa gaacccgag ccgagtccg attcctgaa aatgcaaac ccttttcct acagatcaa gatcaaact gagtaagtt atgttttaa ctatttaat tactttagt ttataacgt tttttttat atttgtatt ccaaaaact ctaataact ctcttcata agactttaa atcttgatg gaacaatca ccatattca tcgcttaag ctttccagg ttttgttag gataattta aacaccaac atacaaaaa gttgtttgg acttaattt acattgacc tgatgatat ttgaaagca aataaaata tttaaaaat tt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
