Status
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Gene information
General information
TC032572
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002594,TC002593 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22385361..22484403
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032572
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00408

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    iBeetle screen iB_07047

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032572 RA
      MATTVAMNE SNHVVYNRT ESTESGWEN 
PFRPGGDLS READEIVEL IKGGKPITP 
TPDSSAPPL PSLDETDTQ KNQTANNLS 
EPITQSPKK ASELNATPK SANGNVKND 
KPNAPGAVD VKRGTIKPD GDVEHVTIK 
KKPKCKCCV IQ
    • >TC032572 RB
      MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS 
SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK 
PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ 
EEPEPSPIP ENANPFPTD QDQTETERE KVRVTFFQQ 
SKPQEVDLP DEPVVFTDA DFIMATTVA MNESNHVVY 
NRTESTESG WENPFRPGG DLSREADEI VELIKGGKP 
ITPTPDSSA PPLPSLDET DTQKNQTAN NLSEPITQS 
PKKASELNA TPKSANGNV KNDKPNAPG AVDVKRGTI 
KPDGDVEHV TIKKKPKCK CCVIQ
    • >TC032572 RC
      MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS 
SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK 
PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ 
EEPEPSPIP ENANPFPTD QDQTEASSK FGNWWFAST 
WRRFSKVSQ CKCFCGRAS SAYQSQSIG VDKEPNEKK 
CE
    • >TC032572 RD
      MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS 
SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK 
PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ 
EEPEPSPIP ENANPFPTD QDQTE
    CDS sequence
    • >TC032572 RA
      atggccacc acggtagct atgaatgaa tcgaatcat 
gtcgtctac aataggacg gaaagtacg gaaagcggc 
tgggagaac cccttccga cctggcggc gacttgagt 
cgagaggcc gacgagata gtagaatta ataaaaggg 
ggcaaaccg ataacgcct acacccgac agttcggcg 
cccccttta ccctcctta gacgaaacc gatacgcaa 
aagaaccaa acagccaac aatctatcg gaacccata 
acacaaagc ccgaagaaa gccagcgaa ctgaatgcc 
accccgaaa agtgctaac ggcaacgtc aaaaacgac 
aaacccaac gctccagga gccgtcgac gttaaacga 
gggacgatt aaaccagac ggagacgtc gaacacgtt 
acgataaaa aagaaaccc aagtgcaag tgctgtgtt 
atacaataa
    • >TC032572 RB
      atgttgtcg tacatgcta ccagtggac gaaaaaccg 
ccaccggct cctaacaga ggacacatg cagttacct 
ttggcttct gaaactcta acaaacggt agaacttca 
agtcctatc agtaaagaa acaagtgtt agcagtttt 
gttagcgat gtgacaaca gcaacagct agtcacagc 
tctatgacg cctacaagc accagtgat aaaggaaag 
cctaagtta gttatcaat ggaggaaag caccaaaca 
ctgaaaagg gtatcgttt ggtagctcc aaggggtcc 
atggttgaa acgcttatt tttgaaagt cctgtccaa 
gaagaaccc gagccgagt ccgattcct gaaaatgca 
aaccctttt cctacagat caagatcaa actgaaacc 
gaacgagaa aaagtgcga gtgacgttc tttcaacaa 
tcaaaacct caagaagtc gatttaccg gatgaacct 
gtcgttttt accgacgct gatttcata atggccacc 
acggtagct atgaatgaa tcgaatcat gtcgtctac 
aataggacg gaaagtacg gaaagcggc tgggagaac 
cccttccga cctggcggc gacttgagt cgagaggcc 
gacgagata gtagaatta ataaaaggg ggcaaaccg 
ataacgcct acacccgac agttcggcg cccccttta 
ccctcctta gacgaaacc gatacgcaa aagaaccaa 
acagccaac aatctatcg gaacccata acacaaagc 
ccgaagaaa gccagcgaa ctgaatgcc accccgaaa 
agtgctaac ggcaacgtc aaaaacgac aaacccaac 
gctccagga gccgtcgac gttaaacga gggacgatt 
aaaccagac ggagacgtc gaacacgtt acgataaaa 
aagaaaccc aagtgcaag tgctgtgtt atacaataa
    • >TC032572 RC
      atgttgtcg tacatgcta ccagtggac gaaaaaccg 
ccaccggct cctaacaga ggacacatg cagttacct 
ttggcttct gaaactcta acaaacggt agaacttca 
agtcctatc agtaaagaa acaagtgtt agcagtttt 
gttagcgat gtgacaaca gcaacagct agtcacagc 
tctatgacg cctacaagc accagtgat aaaggaaag 
cctaagtta gttatcaat ggaggaaag caccaaaca 
ctgaaaagg gtatcgttt ggtagctcc aaggggtcc 
atggttgaa acgcttatt tttgaaagt cctgtccaa 
gaagaaccc gagccgagt ccgattcct gaaaatgca 
aaccctttt cctacagat caagatcaa actgaagca 
tcatcgaag ttcggaaat tggtggttt gcgagtact 
tggcggcgg ttttccaaa gtcagtcag tgcaagtgc 
ttttgtggt cgcgcgtcc tctgcctat caaagccaa 
agcatcggc gtcgataaa gaaccgaac gagaaaaag 
tgcgagtga
    • >TC032572 RD
      atgttgtcg tacatgcta ccagtggac gaaaaaccg 
ccaccggct cctaacaga ggacacatg cagttacct 
ttggcttct gaaactcta acaaacggt agaacttca 
agtcctatc agtaaagaa acaagtgtt agcagtttt 
gttagcgat gtgacaaca gcaacagct agtcacagc 
tctatgacg cctacaagc accagtgat aaaggaaag 
cctaagtta gttatcaat ggaggaaag caccaaaca 
ctgaaaagg gtatcgttt ggtagctcc aaggggtcc 
atggttgaa acgcttatt tttgaaagt cctgtccaa 
gaagaaccc gagccgagt ccgattcct gaaaatgca 
aaccctttt cctacagat caagatcaa actgagtaa
    mRNA sequence
    • >TC032572 RA
      cacccactt gtcggattg tatggtggc gctctgttg 
caaaacagc gaactatcg ccaatccgc gatgtttga 
ggcgtggcg ttcgaagcg tgttgcgta cgagtgtta 
aatatttcg atgtaataa ttttataac ctcgtagag 
catcatcga agttcggaa attggtggt ttgcgagta 
cttggcggc ggttttcca aagtcagtc agtgcaagt 
gcttttgtg gtcgcgcgt cctctgcct atcaaagcc 
aaagcatcg gcgtcgata aagaaccga acgagaaaa 
agtgcgagt gacgttctt tcaacaatc aaaacctca 
agaagtcga tttaccgga tgaacctgt cgtttttac 
cgacgctga tttcataat ggccaccac ggtagctat 
gaatgaatc gaatcatgt cgtctacaa taggacgga 
aagtacgga aagcggctg ggagaaccc cttccgacc 
tggcggcga cttgagtcg agaggccga cgagatagt 
agaattaat aaaaggggg caaaccgat aacgcctac 
acccgacag ttcggcgcc ccctttacc ctccttaga 
cgaaaccga tacgcaaaa gaaccaaac agccaacaa 
tctatcgga acccataac acaaagccc gaagaaagc 
cagcgaact gaatgccac cccgaaaag tgctaacgg 
caacgtcaa aaacgacaa acccaacgc tccaggagc 
cgtcgacgt taaacgagg gacgattaa accagacgg 
agacgtcga acacgttac gataaaaaa gaaacccaa 
gtgcaagtg ctgtgttat acaataact gctgacaga 
tagtcaata gacttgtta ctgttaaat attttataa 
aaaataata gcaaggtgt gagtaaaaa cgattcggt 
tttgcgaga tggtggtgc taactgtga tgtttttag 
tacaaaaaa agcatctga tggccgatc gaccaattc 
gataaaatc ttccacaca aagcagttt tcaacctaa 
cgaaagtaa aaaattgca aacaaaaca tatcaatcc 
agtaataat tttgttgca attaaaaca gtcgatcag 
tggatcaga taatcgccc agaagtaca cgaaataat 
caaaaactg caaccgatt tcaggattt tcgttgaga 
agaaacgaa accgaaggt ttttacgcc acccagagc 
tgagaaatg cttacctat gctttaacc agtcacaca 
aatactagt ggtgcatgt tcaatctct acttaattt 
tcaagcaat actgaatat ttagttgta tggccaata 
ggaattgtc cgccttgtc cgtgacaga aagttttaa 
attcgactc tagtcaaaa ttatttttg tgattgtaa 
acggacaag gtggaacag tgtggttat tggcgctgt 
tattctact aaacgaatg tgattatat acagttata 
aaatttacc atattttta cattgtatt agagttgtt 
tgtgtttcg cgcttattt cttcacggt tgtgggagt 
tttctacag atgagataa acaaacacg gaatatggg 
aacacaaac agcggttta tatttagtt taacatttg 
tataaatat acaatgagt ctagaatgg atgatagta 
caagtattt tttatatat tttagcctt tcatcaaca 
tttttacta ctgtatgac agaaatttc ggattaacg 
gtgtattca gtagcgaga tttagttgt ctatgtttt 
actatttaa cttgtaaat gttctataa ataggtgcg 
acgttctag ttattgttg ttaatatta tttgttgtg 
tttgtgtgt ctggaatga aagtcggaa acatcggca 
ttatttttg gtaaaaaat aatacttga taaagataa 
ttaaatcgt accggaacg atcagacgg ttggtaata 
agtttcatt agtgaaatt tatttgnnn nnnnnnnnn 
nnnnnnnnn nnnaacacg aattgtgca gagattatt 
taatatttt aaatataag tacataacg tccctgttt 
atatgtgta tgtaaagat tgcagctta taatatgct 
taagatgta tagatttta gattgtaaa tcgatcatg 
tttgtttga agcactcca aactctttt ttaatgata 
ttttacatg aggaaattt ttattaata tattatttt 
aatcttttg tgtattata ttgtaatat aaatctaat 
ttgtatacc aagccgcat agcattaaa taatttgtg 
atgtc
    • >TC032572 RB
      tacaaagca gaagtagag tttcgatgg tccgctgtg 
tacaaatcg cgagctgcg ccgtgagta cgtcgcgga 
taaacaccg gctcctcca gctaaaatc gcgctaaga 
ctgcatagc tcctccatc gaattcgaa cggtggtgc 
agtgtaggt gtggtgtcg tggtcaagg gtaagccga 
cacttgccc cagtttcga gcccagatc ggtgcttct 
tcgattgtg accgtgaaa gtgtattag tgtaaaatg 
ttaaaatta agttagtgc ttcgtaatg ttgtcgtac 
atgctacca gtggacgaa aaaccgcca ccggctcct 
aacagagga cacatgcag ttacctttg gcttctgaa 
actctaaca aacggtaga acttcaagt cctatcagt 
aaagaaaca agtgttagc agttttgtt agcgatgtg 
acaacagca acagctagt cacagctct atgacgcct 
acaagcacc agtgataaa ggaaagcct aagttagtt 
atcaatgga ggaaagcac caaacactg aaaagggta 
tcgtttggt agctccaag gggtccatg gttgaaacg 
cttattttt gaaagtcct gtccaagaa gaacccgag 
ccgagtccg attcctgaa aatgcaaac ccttttcct 
acagatcaa gatcaaact gaaaccgaa cgagaaaaa 
gtgcgagtg acgttcttt caacaatca aaacctcaa 
gaagtcgat ttaccggat gaacctgtc gtttttacc 
gacgctgat ttcataatg gccaccacg gtagctatg 
aatgaatcg aatcatgtc gtctacaat aggacggaa 
agtacggaa agcggctgg gagaacccc ttccgacct 
ggcggcgac ttgagtcga gaggccgac gagatagta 
gaattaata aaagggggc aaaccgata acgcctaca 
cccgacagt tcggcgccc cctttaccc tccttagac 
gaaaccgat acgcaaaag aaccaaaca gccaacaat 
ctatcggaa cccataaca caaagcccg aagaaagcc 
agcgaactg aatgccacc ccgaaaagt gctaacggc 
aacgtcaaa aacgacaaa cccaacgct ccaggagcc 
gtcgacgtt aaacgaggg acgattaaa ccagacgga 
gacgtcgaa cacgttacg ataaaaaag aaacccaag 
tgcaagtgc tgtgttata caataactg ctgacagat 
agtcaatag acttgttac tgttaaata ttttataaa 
aaataatag caaggtgtg agtaaaaac gattcggtt 
ttgcgagat ggtggtgct aactgtgat gtttttagt 
acaaaaaaa gcatctgat ggccgatcg accaattcg 
ataaaatct tccacacaa agcagtttt caacctaac 
gaaagtaaa aaattgcaa acaaaacat atcaatcca 
gtaataatt ttgttgcaa ttaaaacag tcgatcagt 
ggatcagat aatcgccca gaagtacac gaaataatc 
aaaaactgc aaccgattt caggatttt cgttgagaa 
gaaacgaaa ccgaaggtt tttacgcca cccagagct 
gagaaatgc ttacctatg ctttaacca gtcacacaa 
atactagtg gtgcatgtt caatctcta cttaatttt 
caagcaata ctgaatatt tagttgtat ggccaatag 
gaattgtcc gccttgtcc gtgacagaa agttttaaa 
ttcgactct agtcaaaat tatttttgt gattgtaaa 
cggacaagg tggaacagt gtggttatt ggcgctgtt 
attctacta aacgaatgt gattatata cagttataa 
aatttacca tatttttac attgtatta gagttgttt 
gtgtttcgc gcttatttc ttcacggtt gtgggagtt 
ttctacaga tgagataaa caaacacgg aatatggga 
acacaaaca gcggtttat atttagttt aacatttgt 
ataaatata caatgagtc tagaatgga tgatagtac 
aagtatttt ttatatatt ttagccttt catcaacat 
ttttactac tgtatgaca gaaatttcg gattaacgg 
tgtattcag tagcgagat ttagttgtc tatgtttta 
ctatttaac ttgtaaatg ttctataaa taggtgcga 
cgttctagt tattgttgt taatattat ttgttgtgt 
ttgtgtgtc tggaatgaa agtcggaaa catcggcat 
tatttttgg taaaaaata atacttgat aaagataat 
taaatcgta ccggaacga tcagacggt tggtaataa 
gtttcatta gtgaaattt atttgnnnn nnnnnnnnn 
nnnnnnnnn nnaacacga attgtgcag agattattt 
aatatttta aatataagt acataacgt ccctgttta 
tatgtgtat gtaaagatt gcagcttat aatatgctt 
aagatgtat agattttag attgtaaat cgatcatgt 
ttgtttgaa gcactccaa actcttttt taatgatat 
tttacatga ggaaatttt tattaatat attatttta 
atcttttgt gtattatat tgtaatata aatctaatt 
tgtatacca agccgcata gcattaaat aatttgtga 
tgtc
    • >TC032572 RC
      tacaaagca gaagtagag tttcgatgg tccgctgtg 
tacaaatcg cgagctgcg ccgtgagta cgtcgcgga 
taaacaccg gctcctcca gctaaaatc gcgctaaga 
ctgcatagc tcctccatc gaattcgaa cggtggtgc 
agtgtaggt gtggtgtcg tggtcaagg gtaagccga 
cacttgccc cagtttcga gcccagatc ggtgcttct 
tcgattgtg accgtgaaa gtgtattag tgtaaaatg 
ttaaaatta agttagtgc ttcgtaatg ttgtcgtac 
atgctacca gtggacgaa aaaccgcca ccggctcct 
aacagagga cacatgcag ttacctttg gcttctgaa 
actctaaca aacggtaga acttcaagt cctatcagt 
aaagaaaca agtgttagc agttttgtt agcgatgtg 
acaacagca acagctagt cacagctct atgacgcct 
acaagcacc agtgataaa ggaaagcct aagttagtt 
atcaatgga ggaaagcac caaacactg aaaagggta 
tcgtttggt agctccaag gggtccatg gttgaaacg 
cttattttt gaaagtcct gtccaagaa gaacccgag 
ccgagtccg attcctgaa aatgcaaac ccttttcct 
acagatcaa gatcaaact gaagcatca tcgaagttc 
ggaaattgg tggtttgcg agtacttgg cggcggttt 
tccaaagtc agtcagtgc aagtgcttt tgtggtcgc 
gcgtcctct gcctatcaa agccaaagc atcggcgtc 
gataaagaa ccgaacgag aaaaagtgc gagtgacgt 
tctttcaac aatcaaaac ctcaagaag tcgatttac 
cggatgaac ctgtcgttt ttaccgacg ctgatttca 
taatggcca ccacggtag ctatgaatg aatcgaatc 
atgtcgtct acaatagga cggaaaggt gagtttttg 
tattgctgt aagtgacta tgtcacact aaaagctca 
caaagact
    • >TC032572 RD
      tacaaagca gaagtagag tttcgatgg tccgctgtg 
tacaaatcg cgagctgcg ccgtgagta cgtcgcgga 
taaacaccg gctcctcca gctaaaatc gcgctaaga 
ctgcatagc tcctccatc gaattcgaa cggtggtgc 
agtgtaggt gtggtgtcg tggtcaagg gtaagccga 
cacttgccc cagtttcga gcccagatc ggtgcttct 
tcgattgtg accgtgaaa gtgtattag tgtaaaatg 
ttaaaatta agttagtgc ttcgtaatg ttgtcgtac 
atgctacca gtggacgaa aaaccgcca ccggctcct 
aacagagga cacatgcag ttacctttg gcttctgaa 
actctaaca aacggtaga acttcaagt cctatcagt 
aaagaaaca agtgttagc agttttgtt agcgatgtg 
acaacagca acagctagt cacagctct atgacgcct 
acaagcacc agtgataaa ggaaagcct aagttagtt 
atcaatgga ggaaagcac caaacactg aaaagggta 
tcgtttggt agctccaag gggtccatg gttgaaacg 
cttattttt gaaagtcct gtccaagaa gaacccgag 
ccgagtccg attcctgaa aatgcaaac ccttttcct 
acagatcaa gatcaaact gagtaagtt atgttttaa 
ctatttaat tactttagt ttataacgt tttttttat 
atttgtatt ccaaaaact ctaataact ctcttcata 
agactttaa atcttgatg gaacaatca ccatattca 
tcgcttaag ctttccagg ttttgttag gataattta 
aacaccaac atacaaaaa gttgtttgg acttaattt 
acattgacc tgatgatat ttgaaagca aataaaata 
tttaaaaat tt
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