Status
Please wait ...
Gene information
General information
TC031053
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002646,TC002645 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16581344..16596887
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031053
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07036

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     abdomen ventral circumference irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03430

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031053 RA
      MYNTPTSKK QKPTTSYLV RPRLGAIRS IYSTKPMSS 
YSQDKKKKL KKSLGKMEY KKKPDYADT AYREAVGRL 
RILLAESYT PIKRPLREL DSAGEDTDN QSLISATSR 
TSKGYRPYY QYLTMPPKR YHYYPSIRP TSVLPPETE 
KNVMNAPKF EGSSAPPEL MSFIEKQEE YIEQLEKES 
RFCRDELSC LLSKVKDVI SENENLHEK QKSNLMKSV 
FSHLETETE TETDVDLAQ KMSLSPKKS KKTRVLEGP 
TIVFESRIS ELEAQLTQA KIDLRKALE DNDNYKKKI 
ADGTIFDGF GFEHYKKQI DALQREKDV LQETISKLQ 
AALAKVRDK ENVTCDQVK RSLDVAEQA QYEKNAAEL 
EIRRLKDEL ERQHSKLRD AISDQSRRI SDERSAVER 
RYTQQIEQL TAELGVQWE TTNKLQLEL DKQRRENAD 
LRREIAQKE ALLDGMRKD MQNKIMTLQ SDIGVNGAE 
KSALEQQIA TLQMTNERN ERQSKQEIG RLQAEMQSL 
RQRLDRADA DLIHSRREN IRLTEQVAS LEKEIKLNS 
ALSEEKSKQ SSGEVLALP PPKEQDKQL SSLIQDMES 
KHAATVAEL EGMIHSQNQ LMDKLSVEC HTLTQKLED 
ASARHKEEI RELQTSLSR LNSRLLNQS QITSSRVKD 
EDYNSPSNQ RTAPPKILS RSNSRSSVK PNPTLSRNN 
SEDKTPSLQ NISDNRTYG SDQPHVGDA SDERNYIPE 
DYQTDPNKE YDQYGNDPN QQYQMDPNQ QYATDPNQQ 
YEMNPNAQY DESYGQYLN DPNVQYVED PNQQYEMNP 
EQQEYVTDP NHPDQQYGT NQQQQEYVT DPNHPDQQY 
GTNQQQQEY GTVKMPPKP KLHKNKGKS DLKFSPSTT 
SSNTTETPP ESSNIATNP ELQEQFETE LCWCIQQLQ 
RSINSGKLN QKQVLESTK VLNMLMNNT TPFIKKRQL 
MRVTFGDYR AKMLEENKK MSKAINIKI SPASPSKKS 
KFVKKSIFS STSDSNFKF NFDVENVED NLSENINKI 
ELTTDNEPK PEAFHFRTS DNSFKFNFV PDES
    • >TC031053 RB
      MYNTPTSKK QKPTTSYLV RPRLGAIRS IYSTKPMSS 
YSQDKKKKL KKSLGKMEY KKKPDYADT AYREAVGRL 
RILLAESYT PIKRPLREL DSAGEDTDN QSLISATSR 
TSKGYRPYY QYLTMPPKR YHYYPSIRP TSVLPPETE 
KNVMNAPKF EGSSAPPEL MSFIEKQEE YIEQLEKES 
RFCRDELSC LLSKVKDVI SENENLHEK QKSNLMKSV 
FSHLETETE TETDVDLAQ KMSLSPKKS KKTRVLEGP 
TIVFESRIS ELEAQLTQA KIDLRKALE DNDNYKKKI 
ADGTIFDGF GFEHYKKQI DALQREKDV LQETISKLQ 
AALAKVRDK ENVTCDQVK RSLDVAEQA QYEKNAAEL 
EIRRLKDEL ERQHSKLRD AISDQSRRI SDERSAVER 
RYTQQIEQL TAELGVQWE TTNKLQLEL DKQRRENAD 
LRREIAQKE ALLDGMRKD MQNKIMTLQ SDIGVNGAE 
KSALEQQIA TLQMTNERN ERQSKQEIG RLQAEMQSL 
RQRLDRADA DLIHSRREN IRLTEQVAS LEKEIKLNS 
ALSEEKSKQ SSGEVLALP PPKEQDKQL SSLIQDMES 
KHAATVAEL EGMIHSQNQ LMDKLSVEC HTLTQKLED 
ASARHKVKD EDYNSPSNQ RTAPPKILS RSNSRSSVK 
PNPTLSRNN SEDKTPSLQ NISDNRTYG SDQPHVGDA 
SDERNYIPE DYQTDPNKE YDQYGNDPN QQYQMDPNQ 
QYATDPNQQ YEMNPNAQY DESYGQYLN DPNVQYVED 
PNQQYEMNP EQQEYVTDP NHPDQQYGT NQQQQEYVT 
DPNHPDQQY GTNQQQQEY GTVKMPPKP KLHKNKGKS 
DLKFSPSTT SSNTTETPP ESSNIATNP ELQEQFETE 
LCWCIQQLQ RSINSGKLN QKQVLESTK VLNMLMNNT 
TPFIKKRQL MRVTFGDYR AKMLEENKK MSKAINIKI 
SPASPSKKS KFVKKSIFS STSDSNFKF NFDVENVED 
NLSENINKI ELTTDNEPK PEAFHFRTS DNSFKFNFV 
PDES
    CDS sequence
    • >TC031053 RA
      atgtataac actccaaca agtaaaaaa caaaaacct 
acaacaagt tacttggtt agaccaaga ttgggtgcc 
atcagaagt atttattca accaaacca atgtcgtct 
tatagtcaa gacaaaaag aaaaaactc aagaaatct 
ctcggcaaa atggaatac aagaaaaag cccgattat 
gccgacact gcgtatcgt gaagccgtt ggaagactt 
cgtattctg ttagcagaa agttacaca ccaattaaa 
agacctttg agggaatta gacagtgca ggagaagac 
actgacaac caatctctc ataagcgct accagcaga 
acgtcaaaa ggatatcgt ccgtactat cagtacctc 
acaatgcct ccaaagcgg tatcactac tatccttcc 
ataagaccg acaagtgta ctaccacca gagaccgaa 
aaaaatgtc atgaatgcg ccaaaattt gaaggcagt 
tctgcgccg ccagagtta atgtctttt atagaaaaa 
caagaggag tatattgag caactggaa aaagaatca 
agattttgc agagatgaa ttatcatgt ttgctgagc 
aaagtcaaa gatgttata agtgaaaat gagaatttg 
cacgaaaag cagaaatcg aatttaatg aagtcggtt 
ttcagtcac ttggagacg gaaacggaa accgaaact 
gacgtggat ttagcccaa aagatgtca ttatcgcct 
aaaaaatcg aaaaaaact cgagtactg gaaggcccc 
acaatcgtt tttgaatca cgaatttcc gaactagaa 
gcccaactg acccaagca aaaatcgat ttaagaaaa 
gctttggaa gataacgac aactacaag aaaaaaatt 
gccgacgga acgattttt gacggattt ggtttcgag 
cattacaag aaacaaatc gatgcattg cagagagaa 
aaagatgtt ttgcaagaa accatttcc aagcttcaa 
gcagcttta gccaaagtt cgagacaaa gaaaacgtt 
acttgcgat caagttaag agaagcttg gatgttgca 
gaacaagca caatatgaa aaaaatgct gcggaactt 
gaaatccga agacttaaa gacgaactg gagcgccaa 
cactccaaa ctcagagac gcaatttca gatcagagt 
cgacgaatc tcagatgag agaagtgct gttgaacgt 
cgatacaca caacaaatc gagcaattg accgccgaa 
ttgggggtt caatgggag actacaaac aaactccaa 
ctggagttg gataagcaa agaagagaa aatgcagat 
ttgagaagg gaaatagca cagaaagag gcactgctt 
gatggcatg cgaaaagac atgcaaaat aaaattatg 
actttgcag tcggatatt ggagtcaac ggagccgaa 
aaaagtgcc ctcgaacag caaattgca acccttcag 
atgaccaac gagcggaat gaacgacaa tcaaagcag 
gaaatcgga cgtttgcag gccgagatg cagtctctg 
agacagaga ttggaccga gcagatgct gatctcatc 
cacagcaga cgggaaaac attcgactc actgaacaa 
gtggcttct ttggaaaaa gaaattaaa ctcaactct 
gcgttgagt gaagagaag agcaaacag tcgagtggc 
gaagttctg gctcttcct ccgcccaaa gagcaagac 
aagcagttg tcttccctc atccaagac atggagtct 
aaacacgcg gctactgta gccgaactc gaaggcatg 
atccattcc caaaatcaa ctcatggac aaattgtca 
gtcgaatgt cacactctt acccagaag ctggaagac 
gccagcgca aggcataag gaggaaata cgggaacta 
caaactagc ctaagtcga ttaaatagt agattgtta 
aatcagtca caaattacc tcctccaga gtcaaagat 
gaggactat aattcccct agcaatcaa agaactgca 
ccaccaaaa atcttgagc cgttccaat agtcgatca 
tccgttaaa ccaaacccc actttatca agaaataac 
agcgaagac aaaacacca tcactccaa aatatttcc 
gataacaga acatacgga tctgatcaa ccccatgtt 
ggtgatgct tcggacgag agaaattat attccagaa 
gattatcaa acggatccc aataaagag tacgatcag 
tacggaaat gatccaaat cagcagtac caaatggat 
ccaaatcaa cagtatgca actgatccg aatcagcaa 
tacgaaatg aatccaaat gcacaatat gatgagtcc 
tacggacaa tacttgaat gatccgaat gttcaatat 
gtcgaggat ccgaatcaa cagtatgaa atgaatcca 
gaacagcaa gagtatgtg acagatccg aatcatccg 
gatcaacag tatggaacg aatcagcaa cagcaagag 
tatgtgacg gatccgaat catcctgat caacagtac 
ggaacgaat caacaacag caagagtat ggaaccgtg 
aaaatgcct ccaaaaccg aaactccac aaaaacaaa 
ggcaaaagt gatttaaaa ttttctcca agtactaca 
agttcaaat acaacagaa acaccaccg gagtcatcg 
aatattgcc actaatccg gaattacaa gagcaattt 
gaaacggaa ctttgctgg tgtattcag caattgcaa 
aggagcatt aattctggt aaactgaat caaaaacaa 
gtgttagaa agtactaaa gtgttaaac atgttgatg 
aataatacg acacctttc ataaaaaag cgtcaactt 
atgagggtt acatttggt gattataga gcaaaaatg 
ttggaagaa aataaaaaa atgtcaaaa gcaatcaac 
ataaagata tcaccggct tcccctagt aaaaaatca 
aaatttgta aaaaaatct atattttcg tcaacatct 
gacagtaat tttaaattc aacttcgac gtggaaaat 
gttgaggat aatttatcc gagaatata aacaagatt 
gaattaact acggacaac gaacctaag ccagaggca 
tttcatttt aggacatct gacaattca tttaaattt 
aatttcgtt cctgatgaa agctaa
    • >TC031053 RB
      atgtataac actccaaca agtaaaaaa caaaaacct 
acaacaagt tacttggtt agaccaaga ttgggtgcc 
atcagaagt atttattca accaaacca atgtcgtct 
tatagtcaa gacaaaaag aaaaaactc aagaaatct 
ctcggcaaa atggaatac aagaaaaag cccgattat 
gccgacact gcgtatcgt gaagccgtt ggaagactt 
cgtattctg ttagcagaa agttacaca ccaattaaa 
agacctttg agggaatta gacagtgca ggagaagac 
actgacaac caatctctc ataagcgct accagcaga 
acgtcaaaa ggatatcgt ccgtactat cagtacctc 
acaatgcct ccaaagcgg tatcactac tatccttcc 
ataagaccg acaagtgta ctaccacca gagaccgaa 
aaaaatgtc atgaatgcg ccaaaattt gaaggcagt 
tctgcgccg ccagagtta atgtctttt atagaaaaa 
caagaggag tatattgag caactggaa aaagaatca 
agattttgc agagatgaa ttatcatgt ttgctgagc 
aaagtcaaa gatgttata agtgaaaat gagaatttg 
cacgaaaag cagaaatcg aatttaatg aagtcggtt 
ttcagtcac ttggagacg gaaacggaa accgaaact 
gacgtggat ttagcccaa aagatgtca ttatcgcct 
aaaaaatcg aaaaaaact cgagtactg gaaggcccc 
acaatcgtt tttgaatca cgaatttcc gaactagaa 
gcccaactg acccaagca aaaatcgat ttaagaaaa 
gctttggaa gataacgac aactacaag aaaaaaatt 
gccgacgga acgattttt gacggattt ggtttcgag 
cattacaag aaacaaatc gatgcattg cagagagaa 
aaagatgtt ttgcaagaa accatttcc aagcttcaa 
gcagcttta gccaaagtt cgagacaaa gaaaacgtt 
acttgcgat caagttaag agaagcttg gatgttgca 
gaacaagca caatatgaa aaaaatgct gcggaactt 
gaaatccga agacttaaa gacgaactg gagcgccaa 
cactccaaa ctcagagac gcaatttca gatcagagt 
cgacgaatc tcagatgag agaagtgct gttgaacgt 
cgatacaca caacaaatc gagcaattg accgccgaa 
ttgggggtt caatgggag actacaaac aaactccaa 
ctggagttg gataagcaa agaagagaa aatgcagat 
ttgagaagg gaaatagca cagaaagag gcactgctt 
gatggcatg cgaaaagac atgcaaaat aaaattatg 
actttgcag tcggatatt ggagtcaac ggagccgaa 
aaaagtgcc ctcgaacag caaattgca acccttcag 
atgaccaac gagcggaat gaacgacaa tcaaagcag 
gaaatcgga cgtttgcag gccgagatg cagtctctg 
agacagaga ttggaccga gcagatgct gatctcatc 
cacagcaga cgggaaaac attcgactc actgaacaa 
gtggcttct ttggaaaaa gaaattaaa ctcaactct 
gcgttgagt gaagagaag agcaaacag tcgagtggc 
gaagttctg gctcttcct ccgcccaaa gagcaagac 
aagcagttg tcttccctc atccaagac atggagtct 
aaacacgcg gctactgta gccgaactc gaaggcatg 
atccattcc caaaatcaa ctcatggac aaattgtca 
gtcgaatgt cacactctt acccagaag ctggaagac 
gccagcgca aggcataaa gtcaaagat gaggactat 
aattcccct agcaatcaa agaactgca ccaccaaaa 
atcttgagc cgttccaat agtcgatca tccgttaaa 
ccaaacccc actttatca agaaataac agcgaagac 
aaaacacca tcactccaa aatatttcc gataacaga 
acatacgga tctgatcaa ccccatgtt ggtgatgct 
tcggacgag agaaattat attccagaa gattatcaa 
acggatccc aataaagag tacgatcag tacggaaat 
gatccaaat cagcagtac caaatggat ccaaatcaa 
cagtatgca actgatccg aatcagcaa tacgaaatg 
aatccaaat gcacaatat gatgagtcc tacggacaa 
tacttgaat gatccgaat gttcaatat gtcgaggat 
ccgaatcaa cagtatgaa atgaatcca gaacagcaa 
gagtatgtg acagatccg aatcatccg gatcaacag 
tatggaacg aatcagcaa cagcaagag tatgtgacg 
gatccgaat catcctgat caacagtac ggaacgaat 
caacaacag caagagtat ggaaccgtg aaaatgcct 
ccaaaaccg aaactccac aaaaacaaa ggcaaaagt 
gatttaaaa ttttctcca agtactaca agttcaaat 
acaacagaa acaccaccg gagtcatcg aatattgcc 
actaatccg gaattacaa gagcaattt gaaacggaa 
ctttgctgg tgtattcag caattgcaa aggagcatt 
aattctggt aaactgaat caaaaacaa gtgttagaa 
agtactaaa gtgttaaac atgttgatg aataatacg 
acacctttc ataaaaaag cgtcaactt atgagggtt 
acatttggt gattataga gcaaaaatg ttggaagaa 
aataaaaaa atgtcaaaa gcaatcaac ataaagata 
tcaccggct tcccctagt aaaaaatca aaatttgta 
aaaaaatct atattttcg tcaacatct gacagtaat 
tttaaattc aacttcgac gtggaaaat gttgaggat 
aatttatcc gagaatata aacaagatt gaattaact 
acggacaac gaacctaag ccagaggca tttcatttt 
aggacatct gacaattca tttaaattt aatttcgtt 
cctgatgaa agctaa
    mRNA sequence
    • >TC031053 RA
      atcttctca tgtcataca caatgtata acactccaa 
caagtaaaa aacaaaaac ctacaacaa gttacttgg 
ttagaccaa gattgggtg ccatcagaa gtatttatt 
caaccaaac caatgtcgt cttatagtc aagacaaaa 
agaaaaaac tcaagaaat ctctcggca aaatggaat 
acaagaaaa agcccgatt atgccgaca ctgcgtatc 
gtgaagccg ttggaagac ttcgtattc tgttagcag 
aaagttaca caccaatta aaagacctt tgagggaat 
tagacagtg caggagaag acactgaca accaatctc 
tcataagcg ctaccagca gaacgtcaa aaggatatc 
gtccgtact atcagtacc tcacaatgc ctccaaagc 
ggtatcact actatcctt ccataagac cgacaagtg 
tactaccac cagagaccg aaaaaaatg tcatgaatg 
cgccaaaat ttgaaggca gttctgcgc cgccagagt 
taatgtctt ttatagaaa aacaagagg agtatattg 
agcaactgg aaaaagaat caagatttt gcagagatg 
aattatcat gtttgctga gcaaagtca aagatgtta 
taagtgaaa atgagaatt tgcacgaaa agcagaaat 
cgaatttaa tgaagtcgg ttttcagtc acttggaga 
cggaaacgg aaaccgaaa ctgacgtgg atttagccc 
aaaagatgt cattatcgc ctaaaaaat cgaaaaaaa 
ctcgagtac tggaaggcc ccacaatcg tttttgaat 
cacgaattt ccgaactag aagcccaac tgacccaag 
caaaaatcg atttaagaa aagctttgg aagataacg 
acaactaca agaaaaaaa ttgccgacg gaacgattt 
ttgacggat ttggtttcg agcattaca agaaacaaa 
tcgatgcat tgcagagag aaaaagatg ttttgcaag 
aaaccattt ccaagcttc aagcagctt tagccaaag 
ttcgagaca aagaaaacg ttacttgcg atcaagtta 
agagaagct tggatgttg cagaacaag cacaatatg 
aaaaaaatg ctgcggaac ttgaaatcc gaagactta 
aagacgaac tggagcgcc aacactcca aactcagag 
acgcaattt cagatcaga gtcgacgaa tctcagatg 
agagaagtg ctgttgaac gtcgataca cacaacaaa 
tcgagcaat tgaccgccg aattggggg ttcaatggg 
agactacaa acaaactcc aactggagt tggataagc 
aaagaagag aaaatgcag atttgagaa gggaaatag 
cacagaaag aggcactgc ttgatggca tgcgaaaag 
acatgcaaa ataaaatta tgactttgc agtcggata 
ttggagtca acggagccg aaaaaagtg ccctcgaac 
agcaaattg caacccttc agatgacca acgagcgga 
atgaacgac aatcaaagc aggaaatcg gacgtttgc 
aggccgaga tgcagtctc tgagacaga gattggacc 
gagcagatg ctgatctca tccacagca gacgggaaa 
acattcgac tcactgaac aagtggctt ctttggaaa 
aagaaatta aactcaact ctgcgttga gtgaagaga 
agagcaaac agtcgagtg gcgaagttc tggctcttc 
ctccgccca aagagcaag acaagcagt tgtcttccc 
tcatccaag acatggagt ctaaacacg cggctactg 
tagccgaac tcgaaggca tgatccatt cccaaaatc 
aactcatgg acaaattgt cagtcgaat gtcacactc 
ttacccaga agctggaag acgccagcg caaggcata 
aggaggaaa tacgggaac tacaaacta gcctaagtc 
gattaaata gtagattgt taaatcagt cacaaatta 
cctcctcca gagtcaaag atgaggact ataattccc 
ctagcaatc aaagaactg caccaccaa aaatcttga 
gccgttcca atagtcgat catccgtta aaccaaacc 
ccactttat caagaaata acagcgaag acaaaacac 
catcactcc aaaatattt ccgataaca gaacatacg 
gatctgatc aaccccatg ttggtgatg cttcggacg 
agagaaatt atattccag aagattatc aaacggatc 
ccaataaag agtacgatc agtacggaa atgatccaa 
atcagcagt accaaatgg atccaaatc aacagtatg 
caactgatc cgaatcagc aatacgaaa tgaatccaa 
atgcacaat atgatgagt cctacggac aatacttga 
atgatccga atgttcaat atgtcgagg atccgaatc 
aacagtatg aaatgaatc cagaacagc aagagtatg 
tgacagatc cgaatcatc cggatcaac agtatggaa 
cgaatcagc aacagcaag agtatgtga cggatccga 
atcatcctg atcaacagt acggaacga atcaacaac 
agcaagagt atggaaccg tgaaaatgc ctccaaaac 
cgaaactcc acaaaaaca aaggcaaaa gtgatttaa 
aattttctc caagtacta caagttcaa atacaacag 
aaacaccac cggagtcat cgaatattg ccactaatc 
cggaattac aagagcaat ttgaaacgg aactttgct 
ggtgtattc agcaattgc aaaggagca ttaattctg 
gtaaactga atcaaaaac aagtgttag aaagtacta 
aagtgttaa acatgttga tgaataata cgacacctt 
tcataaaaa agcgtcaac ttatgaggg ttacatttg 
gtgattata gagcaaaaa tgttggaag aaaataaaa 
aaatgtcaa aagcaatca acataaaga tatcaccgg 
cttccccta gtaaaaaat caaaatttg taaaaaaat 
ctatatttt cgtcaacat ctgacagta attttaaat 
tcaacttcg acgtggaaa atgttgagg ataatttat 
ccgagaata taaacaaga ttgaattaa ctacggaca 
acgaaccta agccagagg catttcatt ttaggacat 
ctgacaatt catttaaat ttaatttcg ttcctgatg 
aaagctaaa atttagttt aaataaata ataattaag 
aac
    • >TC031053 RB
      atcttctca tgtcataca caatgtata acactccaa 
caagtaaaa aacaaaaac ctacaacaa gttacttgg 
ttagaccaa gattgggtg ccatcagaa gtatttatt 
caaccaaac caatgtcgt cttatagtc aagacaaaa 
agaaaaaac tcaagaaat ctctcggca aaatggaat 
acaagaaaa agcccgatt atgccgaca ctgcgtatc 
gtgaagccg ttggaagac ttcgtattc tgttagcag 
aaagttaca caccaatta aaagacctt tgagggaat 
tagacagtg caggagaag acactgaca accaatctc 
tcataagcg ctaccagca gaacgtcaa aaggatatc 
gtccgtact atcagtacc tcacaatgc ctccaaagc 
ggtatcact actatcctt ccataagac cgacaagtg 
tactaccac cagagaccg aaaaaaatg tcatgaatg 
cgccaaaat ttgaaggca gttctgcgc cgccagagt 
taatgtctt ttatagaaa aacaagagg agtatattg 
agcaactgg aaaaagaat caagatttt gcagagatg 
aattatcat gtttgctga gcaaagtca aagatgtta 
taagtgaaa atgagaatt tgcacgaaa agcagaaat 
cgaatttaa tgaagtcgg ttttcagtc acttggaga 
cggaaacgg aaaccgaaa ctgacgtgg atttagccc 
aaaagatgt cattatcgc ctaaaaaat cgaaaaaaa 
ctcgagtac tggaaggcc ccacaatcg tttttgaat 
cacgaattt ccgaactag aagcccaac tgacccaag 
caaaaatcg atttaagaa aagctttgg aagataacg 
acaactaca agaaaaaaa ttgccgacg gaacgattt 
ttgacggat ttggtttcg agcattaca agaaacaaa 
tcgatgcat tgcagagag aaaaagatg ttttgcaag 
aaaccattt ccaagcttc aagcagctt tagccaaag 
ttcgagaca aagaaaacg ttacttgcg atcaagtta 
agagaagct tggatgttg cagaacaag cacaatatg 
aaaaaaatg ctgcggaac ttgaaatcc gaagactta 
aagacgaac tggagcgcc aacactcca aactcagag 
acgcaattt cagatcaga gtcgacgaa tctcagatg 
agagaagtg ctgttgaac gtcgataca cacaacaaa 
tcgagcaat tgaccgccg aattggggg ttcaatggg 
agactacaa acaaactcc aactggagt tggataagc 
aaagaagag aaaatgcag atttgagaa gggaaatag 
cacagaaag aggcactgc ttgatggca tgcgaaaag 
acatgcaaa ataaaatta tgactttgc agtcggata 
ttggagtca acggagccg aaaaaagtg ccctcgaac 
agcaaattg caacccttc agatgacca acgagcgga 
atgaacgac aatcaaagc aggaaatcg gacgtttgc 
aggccgaga tgcagtctc tgagacaga gattggacc 
gagcagatg ctgatctca tccacagca gacgggaaa 
acattcgac tcactgaac aagtggctt ctttggaaa 
aagaaatta aactcaact ctgcgttga gtgaagaga 
agagcaaac agtcgagtg gcgaagttc tggctcttc 
ctccgccca aagagcaag acaagcagt tgtcttccc 
tcatccaag acatggagt ctaaacacg cggctactg 
tagccgaac tcgaaggca tgatccatt cccaaaatc 
aactcatgg acaaattgt cagtcgaat gtcacactc 
ttacccaga agctggaag acgccagcg caaggcata 
aagtcaaag atgaggact ataattccc ctagcaatc 
aaagaactg caccaccaa aaatcttga gccgttcca 
atagtcgat catccgtta aaccaaacc ccactttat 
caagaaata acagcgaag acaaaacac catcactcc 
aaaatattt ccgataaca gaacatacg gatctgatc 
aaccccatg ttggtgatg cttcggacg agagaaatt 
atattccag aagattatc aaacggatc ccaataaag 
agtacgatc agtacggaa atgatccaa atcagcagt 
accaaatgg atccaaatc aacagtatg caactgatc 
cgaatcagc aatacgaaa tgaatccaa atgcacaat 
atgatgagt cctacggac aatacttga atgatccga 
atgttcaat atgtcgagg atccgaatc aacagtatg 
aaatgaatc cagaacagc aagagtatg tgacagatc 
cgaatcatc cggatcaac agtatggaa cgaatcagc 
aacagcaag agtatgtga cggatccga atcatcctg 
atcaacagt acggaacga atcaacaac agcaagagt 
atggaaccg tgaaaatgc ctccaaaac cgaaactcc 
acaaaaaca aaggcaaaa gtgatttaa aattttctc 
caagtacta caagttcaa atacaacag aaacaccac 
cggagtcat cgaatattg ccactaatc cggaattac 
aagagcaat ttgaaacgg aactttgct ggtgtattc 
agcaattgc aaaggagca ttaattctg gtaaactga 
atcaaaaac aagtgttag aaagtacta aagtgttaa 
acatgttga tgaataata cgacacctt tcataaaaa 
agcgtcaac ttatgaggg ttacatttg gtgattata 
gagcaaaaa tgttggaag aaaataaaa aaatgtcaa 
aagcaatca acataaaga tatcaccgg cttccccta 
gtaaaaaat caaaatttg taaaaaaat ctatatttt 
cgtcaacat ctgacagta attttaaat tcaacttcg 
acgtggaaa atgttgagg ataatttat ccgagaata 
taaacaaga ttgaattaa ctacggaca acgaaccta 
agccagagg catttcatt ttaggacat ctgacaatt 
catttaaat ttaatttcg ttcctgatg aaagctaaa 
atttagttt aaataaata ataattaag aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY