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Gene information
General information
TC003789 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17101708..17129664
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003789
    iBeetle screen iB_07032

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    maxilla number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible potentially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003789 RA
      MPFISKDWR SPGEAWVKT QEGWEKKKV LENCKAIKQ 
      SGAEEPSTY GNTETAIQP HCHITIKST KEIAGFNEL 
      NEAVKKLDF MSAVRDVRR FNYICALLG LLIGQQMTA 
      LSGCAQKVL LAMLEEVAS QAVASQHNP RNFRQLLKN 
      LRSLSEAER SSCWGGLLG SQVLWSEHT AKIERILKM 
      AANMQICEL SPESRPQLL QLPEECIRE IILRLSDHR 
      DLTASAQTC EQMATIVGE QRVWRELTK FHFTPHQIE 
      LVLPKNGEK VDWKDVYHS LKKSFGLSE DRQYAEMLS 
      LCRYCRCLF WRSSGHPCI ADQCPEFRA RLQEAGGIT 
      PPSPVPPSA FLKFFSL
    CDS sequence
    • >TC003789 RA
      atgcctttt atatcgaag gattggcgg tcaccgggg 
      gaagcctgg gtcaaaaca caggaagga tgggagaag 
      aaaaaagtg ctggaaaac tgcaaagcc atcaaacaa 
      agtggcgca gaagaacct tctacgtac ggtaacacc 
      gaaactgcc atccaaccc cattgccac attacaata 
      aaatccaca aaagaaata gctggattt aacgaacta 
      aacgaggct gtaaaaaaa ctggacttc atgagcgct 
      gtgagagac gtccgccgc ttcaactac atttgcgct 
      ttactaggc ttactcatc ggtcaacaa atgaccgca 
      ctgtcgggc tgcgcccaa aaagtgctt ttagccatg 
      ttggaagaa gtcgcaagc caggcggta gcaagccaa 
      cacaatccc cgaaatttc agacaactg ctcaaaaat 
      ttaagatcg ttgagtgag gccgaacgc tcctcctgc 
      tggggcggc ctcctcggc tcccaagtg ctgtggagc 
      gaacacacg gcgaaaatt gaacgcatc ctcaaaatg 
      gcagctaat atgcaaatc tgcgaactc agcccggag 
      tcgaggccg caattgttg cagctgccg gaggagtgc 
      atccgggag attattcta agattgtcg gatcataga 
      gacttgaca gctagtgcc caaacgtgc gagcaaatg 
      gccacaatt gtgggcgag cagcgcgtc tggagagag 
      ttaaccaag ttccacttt acacctcat caaatagag 
      ctagtttta ccaaaaaac ggcgagaaa gttgactgg 
      aaggatgtt tatcactca ctcaaaaag tccttcgga 
      ttaagcgaa gaccgtcag tacgcagaa atgctgtcg 
      ttgtgtaga tactgtcga tgcctgttt tggcggtct 
      tccggtcat ccgtgcata gccgatcag tgcccggaa 
      ttccgggcg agacttcaa gaagcggga ggaattact 
      ccgcccagt cctgttcct ccttcggcc tttctgaag 
      tttttctcc ttgtag
    mRNA sequence
    • >TC003789 RA
      tttgtttgt tgaaaactt aaataattc catttcaaa 
      atctttcaa aatactcct aattaaagt aacaagcat 
      ccgttcgtt tattacgcg ataataaag cgtattact 
      cagttcact gccttcata accacgtta ttaaattta 
      gggtaaaac actccaaag tcgtttatg taagtgttt 
      agcatatcc taggttatt attaaattt gaagacgca 
      gtgagtgtt ccttccacc tacttcact tgaagagaa 
      aggtgaacg aactacgct aaaagtaaa tgtttaatg 
      actaaaacc atgattcgt ccaatcaac tcggctcgc 
      cataacaaa aacacatgg ttcaatttg gaactgctg 
      gagaagaca ttcatctca ggcagcagg aaacgacca 
      tgcgcacac tcctgctgc ctgctgtag aatcagctg 
      attgcgcag aaaaccaga atcgcgttt ccataatca 
      aacgagcga ctgtcaaag tgttgtgta tatttacat 
      cagctgttt ggctgaacc gtttaaaaa ataatgcct 
      tttatatcg aaggattgg cggtcaccg ggggaagcc 
      tgggtcaaa acacaggaa ggatgggag aagaaaaaa 
      gtgctggaa aactgcaaa gccatcaaa caaagtggc 
      gcagaagaa ccttctacg tacggtaac accgaaact 
      gccatccaa ccccattgc cacattaca ataaaatcc 
      acaaaagaa atagctgga tttaacgaa ctaaacgag 
      gctgtaaaa aaactggac ttcatgagc gctgtgaga 
      gacgtccgc cgcttcaac tacatttgc gctttacta 
      ggcttactc atcggtcaa caaatgacc gcactgtcg 
      ggctgcgcc caaaaagtg cttttagcc atgttggaa 
      gaagtcgca agccaggcg gtagcaagc caacacaat 
      ccccgaaat ttcagacaa ctgctcaaa aatttaaga 
      tcgttgagt gaggccgaa cgctcctcc tgctggggc 
      ggcctcctc ggctcccaa gtgctgtgg agcgaacac 
      acggcgaaa attgaacgc atcctcaaa atggcagct 
      aatatgcaa atctgcgaa ctcagcccg gagtcgagg 
      ccgcaattg ttgcagctg ccggaggag tgcatccgg 
      gagattatt ctaagattg tcggatcat agagacttg 
      acagctagt gcccaaacg tgcgagcaa atggccaca 
      attgtgggc gagcagcgc gtctggaga gagttaacc 
      aagttccac tttacacct catcaaata gagctagtt 
      ttaccaaaa aacggcgag aaagttgac tggaaggat 
      gtttatcac tcactcaaa aagtccttc ggattaagc 
      gaagaccgt cagtacgca gaaatgctg tcgttgtgt 
      agatactgt cgatgcctg ttttggcgg tcttccggt 
      catccgtgc atagccgat cagtgcccg gaattccgg 
      gcgagactt caagaagcg ggaggaatt actccgccc 
      agtcctgtt cctccttcg gcctttctg aagtttttc 
      tccttgtag ttgccttcg tccaattaa tttattatg 
      cttctgttg cattgtgat acataactg tgtattttt 
      aggggccgc tatctcatg tagatatat cgaatttac 
      aaattcata tttttacgt acctactct atagtgtgt 
      aattttaca agatatcaa atatcggta aggatatat 
      ttttcattg ggaaaatag cttgagtga taagtggct 
      gatattttg ctttaaaga aacgaagtc tgaagtgat 
      tattttttc aaatagatg taactctca gtgacgatg 
      tttgttcgc caacaaaca gcaaacggt gtgacttta 
      tagtgatat acaggctgt ctcagctaa cactttcga 
      gcttaatat ctcagttat ttatcaacg aattgttat 
      gaaatttag aatgcacat attttagga ggtggtctt 
      tcaattagg ggcaaaaat gacctcaag ttaaaaaat 
      gaaatttta aaacacatt tcttttatt taaaattaa 
      gccaaatta ccgttggat cattaaacc ccgaaaaat 
      aaatggcta cccaaaaaa ttaaccaaa tcgctcggc 
      tgatccaag aaaaaaatt aaattttgt tggtaaaaa 
      cttaatcca aattttgat aataatttg ggcagtcac 
      cgtttgaaa caattgatg aagcgtttc tatgcggca 
      ttttgtgta ccactgaaa aaaactgtc aaaagtgtg 
      gcgctgtca gaaaagtaa aattgtttt aatttgatg 
      aaattactt taaaaacca acaacagtg gctgaaatt 
      tctgtgttg ttgaaaaag aaatcagta ttcgcctat 
      ggaaagtgt cgttggcct ttttgtgaa atgttattt 
      atgaaactt aaagttcaa agaatcgtc aataatttg 
      aagacacag gaagcgaac ctgaactaa atggagtga 
      ggacaaaac acgtcactg gaaacgaag caatagttg 
      gggctgtaa ttcaagctt ttgaagaga atccaatca 
      gcagtgtac gaaacattt ccaaagtct cgaatgttc 
      aagtgtcta gaatttttc agttgttgt tcagtcttt 
      tagtctttt ttgaaagat ttaaagcat ccagtacca 
      aaagtaagt caaaaatat tgtttttta actttgtat 
      aggtgagag ataattgtg aaatgtctc ttgcgtgac 
      agttacgtt tctgcgagt aggattgac caaaattca 
      atggtagtg ttcatttgt ttcatagag atctaggct 
      tttgcattt gtttaacag tttgtttct catttttca 
      cgcgaaaca gatgtcttt cacgaacga gttttttct 
      gacagcatt tttcactga cgataattt ttttaatat 
      aagtcttac aaggct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY