General information | |||
TC003789 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 17101708..17129664 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003789 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07032 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
maxilla number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
mandible potentially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC003789 RA
MPFISKDWR SPGEAWVKT QEGWEKKKV LENCKAIKQ SGAEEPSTY GNTETAIQP HCHITIKST KEIAGFNEL NEAVKKLDF MSAVRDVRR FNYICALLG LLIGQQMTA LSGCAQKVL LAMLEEVAS QAVASQHNP RNFRQLLKN LRSLSEAER SSCWGGLLG SQVLWSEHT AKIERILKM AANMQICEL SPESRPQLL QLPEECIRE IILRLSDHR DLTASAQTC EQMATIVGE QRVWRELTK FHFTPHQIE LVLPKNGEK VDWKDVYHS LKKSFGLSE DRQYAEMLS LCRYCRCLF WRSSGHPCI ADQCPEFRA RLQEAGGIT PPSPVPPSA FLKFFSL
- >TC003789 RA
atgcctttt atatcgaag gattggcgg tcaccgggg gaagcctgg gtcaaaaca caggaagga tgggagaag aaaaaagtg ctggaaaac tgcaaagcc atcaaacaa agtggcgca gaagaacct tctacgtac ggtaacacc gaaactgcc atccaaccc cattgccac attacaata aaatccaca aaagaaata gctggattt aacgaacta aacgaggct gtaaaaaaa ctggacttc atgagcgct gtgagagac gtccgccgc ttcaactac atttgcgct ttactaggc ttactcatc ggtcaacaa atgaccgca ctgtcgggc tgcgcccaa aaagtgctt ttagccatg ttggaagaa gtcgcaagc caggcggta gcaagccaa cacaatccc cgaaatttc agacaactg ctcaaaaat ttaagatcg ttgagtgag gccgaacgc tcctcctgc tggggcggc ctcctcggc tcccaagtg ctgtggagc gaacacacg gcgaaaatt gaacgcatc ctcaaaatg gcagctaat atgcaaatc tgcgaactc agcccggag tcgaggccg caattgttg cagctgccg gaggagtgc atccgggag attattcta agattgtcg gatcataga gacttgaca gctagtgcc caaacgtgc gagcaaatg gccacaatt gtgggcgag cagcgcgtc tggagagag ttaaccaag ttccacttt acacctcat caaatagag ctagtttta ccaaaaaac ggcgagaaa gttgactgg aaggatgtt tatcactca ctcaaaaag tccttcgga ttaagcgaa gaccgtcag tacgcagaa atgctgtcg ttgtgtaga tactgtcga tgcctgttt tggcggtct tccggtcat ccgtgcata gccgatcag tgcccggaa ttccgggcg agacttcaa gaagcggga ggaattact ccgcccagt cctgttcct ccttcggcc tttctgaag tttttctcc ttgtag
- >TC003789 RA
tttgtttgt tgaaaactt aaataattc catttcaaa atctttcaa aatactcct aattaaagt aacaagcat ccgttcgtt tattacgcg ataataaag cgtattact cagttcact gccttcata accacgtta ttaaattta gggtaaaac actccaaag tcgtttatg taagtgttt agcatatcc taggttatt attaaattt gaagacgca gtgagtgtt ccttccacc tacttcact tgaagagaa aggtgaacg aactacgct aaaagtaaa tgtttaatg actaaaacc atgattcgt ccaatcaac tcggctcgc cataacaaa aacacatgg ttcaatttg gaactgctg gagaagaca ttcatctca ggcagcagg aaacgacca tgcgcacac tcctgctgc ctgctgtag aatcagctg attgcgcag aaaaccaga atcgcgttt ccataatca aacgagcga ctgtcaaag tgttgtgta tatttacat cagctgttt ggctgaacc gtttaaaaa ataatgcct tttatatcg aaggattgg cggtcaccg ggggaagcc tgggtcaaa acacaggaa ggatgggag aagaaaaaa gtgctggaa aactgcaaa gccatcaaa caaagtggc gcagaagaa ccttctacg tacggtaac accgaaact gccatccaa ccccattgc cacattaca ataaaatcc acaaaagaa atagctgga tttaacgaa ctaaacgag gctgtaaaa aaactggac ttcatgagc gctgtgaga gacgtccgc cgcttcaac tacatttgc gctttacta ggcttactc atcggtcaa caaatgacc gcactgtcg ggctgcgcc caaaaagtg cttttagcc atgttggaa gaagtcgca agccaggcg gtagcaagc caacacaat ccccgaaat ttcagacaa ctgctcaaa aatttaaga tcgttgagt gaggccgaa cgctcctcc tgctggggc ggcctcctc ggctcccaa gtgctgtgg agcgaacac acggcgaaa attgaacgc atcctcaaa atggcagct aatatgcaa atctgcgaa ctcagcccg gagtcgagg ccgcaattg ttgcagctg ccggaggag tgcatccgg gagattatt ctaagattg tcggatcat agagacttg acagctagt gcccaaacg tgcgagcaa atggccaca attgtgggc gagcagcgc gtctggaga gagttaacc aagttccac tttacacct catcaaata gagctagtt ttaccaaaa aacggcgag aaagttgac tggaaggat gtttatcac tcactcaaa aagtccttc ggattaagc gaagaccgt cagtacgca gaaatgctg tcgttgtgt agatactgt cgatgcctg ttttggcgg tcttccggt catccgtgc atagccgat cagtgcccg gaattccgg gcgagactt caagaagcg ggaggaatt actccgccc agtcctgtt cctccttcg gcctttctg aagtttttc tccttgtag ttgccttcg tccaattaa tttattatg cttctgttg cattgtgat acataactg tgtattttt aggggccgc tatctcatg tagatatat cgaatttac aaattcata tttttacgt acctactct atagtgtgt aattttaca agatatcaa atatcggta aggatatat ttttcattg ggaaaatag cttgagtga taagtggct gatattttg ctttaaaga aacgaagtc tgaagtgat tattttttc aaatagatg taactctca gtgacgatg tttgttcgc caacaaaca gcaaacggt gtgacttta tagtgatat acaggctgt ctcagctaa cactttcga gcttaatat ctcagttat ttatcaacg aattgttat gaaatttag aatgcacat attttagga ggtggtctt tcaattagg ggcaaaaat gacctcaag ttaaaaaat gaaatttta aaacacatt tcttttatt taaaattaa gccaaatta ccgttggat cattaaacc ccgaaaaat aaatggcta cccaaaaaa ttaaccaaa tcgctcggc tgatccaag aaaaaaatt aaattttgt tggtaaaaa cttaatcca aattttgat aataatttg ggcagtcac cgtttgaaa caattgatg aagcgtttc tatgcggca ttttgtgta ccactgaaa aaaactgtc aaaagtgtg gcgctgtca gaaaagtaa aattgtttt aatttgatg aaattactt taaaaacca acaacagtg gctgaaatt tctgtgttg ttgaaaaag aaatcagta ttcgcctat ggaaagtgt cgttggcct ttttgtgaa atgttattt atgaaactt aaagttcaa agaatcgtc aataatttg aagacacag gaagcgaac ctgaactaa atggagtga ggacaaaac acgtcactg gaaacgaag caatagttg gggctgtaa ttcaagctt ttgaagaga atccaatca gcagtgtac gaaacattt ccaaagtct cgaatgttc aagtgtcta gaatttttc agttgttgt tcagtcttt tagtctttt ttgaaagat ttaaagcat ccagtacca aaagtaagt caaaaatat tgtttttta actttgtat aggtgagag ataattgtg aaatgtctc ttgcgtgac agttacgtt tctgcgagt aggattgac caaaattca atggtagtg ttcatttgt ttcatagag atctaggct tttgcattt gtttaacag tttgtttct catttttca cgcgaaaca gatgtcttt cacgaacga gttttttct gacagcatt tttcactga cgataattt ttttaatat aagtcttac aaggct
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
