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Gene information
General information
TC002668 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17798901..17806652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002668
    iBeetle screen iB_07025

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment:
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva dorsal pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages structure irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment:
    head appendages size shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna antennal flagellum mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs size shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Leg shape is irregular & can not differenciate between Femur & Tt.
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs segment partially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment partially shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002668 RA
      MSSSSDSEE FYDAEDLTP SHGPRKNRK SSKESLTQR 
      ASSEPNVST VPPGHDERQ LQSVNEIVF VEPQKRTPL 
      GGRRKFKEL RQKLQHDDE DCQTGNVTP PNSQTSSVE 
      GVFAASNKT SHPFRIIEQ DTVSLQSIN SLGRLTRIL 
      GGIAEGECG RKAVSVSAL SSSSLDSGS TPSSKGISQ 
      YPSNATLTS EKSSLPLQE PDVIASTKA SSSQKSDDQ 
      NVAPVAPPR RKKKNKAAL QIKKSASSA TFESSDLAS 
      PASTIESLT REFEHSLDR LPSGNESQS STLRSGHSL 
      DIKQALKGQ FVVKPQDNE SLKAGEGGK VKSEVVQAA 
      SPRGSSTRS SVGHYSLGP HRGTHQSNN RSTKERRKS 
      AGDENMLKD ANLKVRTHT DSGKQLSDF EILEQVTVL 
      NLDTGERVP LSVAEDKLP QCINPLSLH IMRLTSEYV 
      SSSSMEKEK ESDEESEEK KIEIPIVDD NEDVSRMRK 
      KTAMLKKFV GTAMKKTMH KAKSIAQEV SHARHKEDI 
      IDIVDVVNP GEQTIKLKA SSSHKGPYE FEKVEHVQE 
      LKDEHEGPI WCMKFSCCG RLLATAGQD KVLRIWIVR 
      DAFPFFQDM RTKYNAEKV SPTPSQESL VSHHSGENS 
      NLAVLEAMT SEECGKMMF MPKPFCTYT GHTSDLLDV 
      SWSKNYFIL SSSMDKTVR LWHISRKEC LCCFQHIDF 
      VTAIVFHPR DDRYFLSGS LDGKLRLWN IPDKKVAVW 
      NEVEGNPKL ITAANFCQN GKFAVVGTY DGRCIFYIT 
      DQLKYHTQI HVRSSRGRN ATGRKISGI EPMPGEDKI 
      LVTSNDSRI RLYDLRDLN VSCKYKGYV NMSSQIKAS 
      FSHDGKYIV SGSENRDIY IWKTNHDYA KFSSVRRDR 
      NDFWEAIKA HNAVVTCAI FAPNPEGVI KMIEESCKS 
      EETETNDKQ VEDPVVEQH TKGCGGYVL ISADFNGCI 
      KVFINKMKP KHSSLPVSA MVSNTKIKK T
    CDS sequence
    • >TC002668 RA
      atgtccagc agcagcgac tcggaggaa ttctacgac 
      gccgaagac ctaacacct agtcatggt ccaagaaaa 
      aatcggaaa agctccaag gaaagtctc acccagagg 
      gctagttcg gaacctaat gtctcaacg gtgccccca 
      ggtcacgac gagagacag ctccagtcg gtcaatgag 
      attgttttt gttgagccc cagaagagg acaccgctt 
      ggtggccgg cgcaagttc aaggagttg agacagaag 
      ctgcaacac gacgatgag gactgtcaa actggcaac 
      gttacgccc cccaacagc caaacatca tcagttgag 
      ggggttttc gccgcctcc aataagacc tcccaccct 
      ttccgtatc atagaacag gacactgtg agcctccag 
      agtatcaat tctttagga cggctcaca cgaatttta 
      ggggggatc gccgaaggg gaatgtggc cgcaaggcc 
      gtttccgta tccgctctc tcctccagc tccctcgac 
      tcgggctcc acccccagc tcgaagggc atctcccag 
      taccccagc aatgcaaca ctgacttcc gaaaagtcc 
      agtcttccg ctacaagaa cccgacgtc attgcaagc 
      acaaaagcc tcctcttcg caaaagagc gacgatcaa 
      aatgtggca ccggtggct cccccacgt cgcaaaaag 
      aaaaacaag gcggcgctc caaatcaaa aaatctgca 
      tcgagtgcg acttttgag tcctccgac ctggcgagt 
      cctgcgagt accattgag tctttgacg cgcgaattc 
      gaacattct ctcgatcgc ctgccgtcc ggcaacgaa 
      tcgcagagt tcgacgcta cggtcgggg cactcgttg 
      gatatcaag caggcgttg aagggtcaa tttgtggtc 
      aagccccag gataatgaa agtttgaag gccggggaa 
      gggggtaag gttaagagt gaagtggtc caagcagcg 
      agtccgaga gggtcttcc acgaggtca agtgtcggg 
      cactacagt ttgggacct catagagga acacaccag 
      tcgaacaat cggagtaca aaagagcgt cggaaatcc 
      gccggtgac gaaaacatg ttaaaagat gcgaattta 
      aaagttcgg actcacacc gactccggt aaacaatta 
      tccgacttc gagattttg gagcaagtg actgtgctc 
      aatctagat accggagaa cgagtccct ttgagcgta 
      gccgaagac aagctacct caatgcatc aatccctta 
      tccctccac ataatgcga ctaacctcc gaatacgtg 
      agcagctca agcatggag aaagagaaa gaatcggac 
      gaagaaagc gaagagaaa aaaatcgag atccccatc 
      gtcgacgac aatgaagac gtgagtcgg atgcggaag 
      aagacggcc atgttgaag aagttcgta ggcaccgcg 
      atgaagaaa acaatgcac aaggccaaa agcatagcc 
      caggaggtg tcccacgcc cgccacaag gaggacatc 
      atcgatatc gtcgatgtt gtcaatcct ggggagcaa 
      acaatcaaa ctcaaggct tccagctcg cacaaaggc 
      ccctacgag ttcgagaaa gtcgagcat gtgcaagag 
      ctcaaagac gagcacgaa gggcccatc tggtgcatg 
      aagttctcc tgctgtggg cgtctcctg gccacagcg 
      ggccaagat aaagtgttg cggatttgg atcgtccgt 
      gacgctttt cccttcttc caagacatg cggaccaaa 
      tacaacgcc gagaaggtg tcgccaact ccgtcgcag 
      gaatcgcta gtgtcgcac cattcgggc gagaacagc 
      aatttggcg gtgttggaa gctatgact tccgaggag 
      tgtggcaag atgatgttt atgccgaag cccttttgt 
      acatatacg gggcatacg agcgacttg ttggatgtt 
      tcatggtcg aagaattac ttcattttg tcgagttcc 
      atggataag acggttagg ttgtggcat atttcgagg 
      aaggagtgt ttgtgttgt ttccaacat attgatttt 
      gtgacggcg attgtgttc catccaagg gatgacaga 
      tactttttg agcggttca ctggacggc aaactccgt 
      ctgtggaac atcccggac aagaaagtg gccgtttgg 
      aacgaagtc gaaggcaac ccgaaacta ataaccgcg 
      gcaaacttt tgccaaaac ggcaaattc gccgttgtg 
      ggtacatac gacggtcgt tgcatcttt tacataacc 
      gatcaatta aaatatcac acacaaatc catgtcagg 
      tcttcccgt ggccgaaac gccaccggt cgcaaaatc 
      agcggcata gaaccgatg cctggagag gataaaatt 
      ttggtcact tcgaacgac agtcggatt cggttgtac 
      gacttgaga gacttgaac gtctcgtgc aaatacaag 
      ggatatgta aatatgagt agccagatt aaggcgagt 
      tttagccac gacggtaaa tacatcgtg agtggctcg 
      gagaatagg gatatttac atttggaag actaaccac 
      gactatgcg aagttttcg tcagtgcgg agggatcgc 
      aacgatttt tgggaagcc atcaaagct cataatgcg 
      gttgtgacg tgtgccatt ttcgcacca aatcccgaa 
      ggggtcatc aagatgatt gaggagagc tgcaagtcg 
      gaagaaacg gaaactaac gataaacag gtggaggat 
      ccggtggtg gaacaacat accaaaggt tgtggcggt 
      tatgtgtta atctcggcc gatttcaac ggctgcatc 
      aaagtcttc atcaataaa atgaaaccc aagcatagc 
      tcattacca gtctccgca atggtgtcc aacaccaaa 
      attaaaaaa acctag
    mRNA sequence
    • >TC002668 RA
      actgacagg cccagcggc gctggcgcc gaaatttgc 
      gagtgcccc cgtgacaac gccccgaaa tgacgagtc 
      gctcatgag acaatgtcc agcagcagc gactcggag 
      gaattctac gacgccgaa gacctaaca cctagtcat 
      ggtccaaga aaaaatcgg aaaagctcc aaggaaagt 
      ctcacccag agggctagt tcggaacct aatgtctca 
      acggtgccc ccaggtcac gacgagaga cagctccag 
      tcggtcaat gagattgtt tttgttgag ccccagaag 
      aggacaccg cttggtggc cggcgcaag ttcaaggag 
      ttgagacag aagctgcaa cacgacgat gaggactgt 
      caaactggc aacgttacg ccccccaac agccaaaca 
      tcatcagtt gagggggtt ttcgccgcc tccaataag 
      acctcccac cctttccgt atcatagaa caggacact 
      gtgagcctc cagagtatc aattcttta ggacggctc 
      acacgaatt ttagggggg atcgccgaa ggggaatgt 
      ggccgcaag gccgtttcc gtatccgct ctctcctcc 
      agctccctc gactcgggc tccaccccc agctcgaag 
      ggcatctcc cagtacccc agcaatgca acactgact 
      tccgaaaag tccagtctt ccgctacaa gaacccgac 
      gtcattgca agcacaaaa gcctcctct tcgcaaaag 
      agcgacgat caaaatgtg gcaccggtg gctccccca 
      cgtcgcaaa aagaaaaac aaggcggcg ctccaaatc 
      aaaaaatct gcatcgagt gcgactttt gagtcctcc 
      gacctggcg agtcctgcg agtaccatt gagtctttg 
      acgcgcgaa ttcgaacat tctctcgat cgcctgccg 
      tccggcaac gaatcgcag agttcgacg ctacggtcg 
      gggcactcg ttggatatc aagcaggcg ttgaagggt 
      caatttgtg gtcaagccc caggataat gaaagtttg 
      aaggccggg gaagggggt aaggttaag agtgaagtg 
      gtccaagca gcgagtccg agagggtct tccacgagg 
      tcaagtgtc gggcactac agtttggga cctcataga 
      ggaacacac cagtcgaac aatcggagt acaaaagag 
      cgtcggaaa tccgccggt gacgaaaac atgttaaaa 
      gatgcgaat ttaaaagtt cggactcac accgactcc 
      ggtaaacaa ttatccgac ttcgagatt ttggagcaa 
      gtgactgtg ctcaatcta gataccgga gaacgagtc 
      cctttgagc gtagccgaa gacaagcta cctcaatgc 
      atcaatccc ttatccctc cacataatg cgactaacc 
      tccgaatac gtgagcagc tcaagcatg gagaaagag 
      aaagaatcg gacgaagaa agcgaagag aaaaaaatc 
      gagatcccc atcgtcgac gacaatgaa gacgtgagt 
      cggatgcgg aagaagacg gccatgttg aagaagttc 
      gtaggcacc gcgatgaag aaaacaatg cacaaggcc 
      aaaagcata gcccaggag gtgtcccac gcccgccac 
      aaggaggac atcatcgat atcgtcgat gttgtcaat 
      cctggggag caaacaatc aaactcaag gcttccagc 
      tcgcacaaa ggcccctac gagttcgag aaagtcgag 
      catgtgcaa gagctcaaa gacgagcac gaagggccc 
      atctggtgc atgaagttc tcctgctgt gggcgtctc 
      ctggccaca gcgggccaa gataaagtg ttgcggatt 
      tggatcgtc cgtgacgct tttcccttc ttccaagac 
      atgcggacc aaatacaac gccgagaag gtgtcgcca 
      actccgtcg caggaatcg ctagtgtcg caccattcg 
      ggcgagaac agcaatttg gcggtgttg gaagctatg 
      acttccgag gagtgtggc aagatgatg tttatgccg 
      aagcccttt tgtacatat acggggcat acgagcgac 
      ttgttggat gtttcatgg tcgaagaat tacttcatt 
      ttgtcgagt tccatggat aagacggtt aggttgtgg 
      catatttcg aggaaggag tgtttgtgt tgtttccaa 
      catattgat tttgtgacg gcgattgtg ttccatcca 
      agggatgac agatacttt ttgagcggt tcactggac 
      ggcaaactc cgtctgtgg aacatcccg gacaagaaa 
      gtggccgtt tggaacgaa gtcgaaggc aacccgaaa 
      ctaataacc gcggcaaac ttttgccaa aacggcaaa 
      ttcgccgtt gtgggtaca tacgacggt cgttgcatc 
      ttttacata accgatcaa ttaaaatat cacacacaa 
      atccatgtc aggtcttcc cgtggccga aacgccacc 
      ggtcgcaaa atcagcggc atagaaccg atgcctgga 
      gaggataaa attttggtc acttcgaac gacagtcgg 
      attcggttg tacgacttg agagacttg aacgtctcg 
      tgcaaatac aagggatat gtaaatatg agtagccag 
      attaaggcg agttttagc cacgacggt aaatacatc 
      gtgagtggc tcggagaat agggatatt tacatttgg 
      aagactaac cacgactat gcgaagttt tcgtcagtg 
      cggagggat cgcaacgat ttttgggaa gccatcaaa 
      gctcataat gcggttgtg acgtgtgcc attttcgca 
      ccaaatccc gaaggggtc atcaagatg attgaggag 
      agctgcaag tcggaagaa acggaaact aacgataaa 
      caggtggag gatccggtg gtggaacaa cataccaaa 
      ggttgtggc ggttatgtg ttaatctcg gccgatttc 
      aacggctgc atcaaagtc ttcatcaat aaaatgaaa 
      cccaagcat agctcatta ccagtctcc gcaatggtg 
      tccaacacc aaaattaaa aaaacctag agtagctct 
      aattttatt actaaattg tgatcgatt gtattatat 
      tttttttaa attgtacat tattttatc cagttctaa 
      taaacacta attgagaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY