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Gene information
General information
TC002688 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18616938..18628096
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002688
    iBeetle screen iB_07021

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002688 RA
      MFPRGVKQL GRLRPCSLG ILPSSVCRG FASPTLPEL 
      TKDRYPHVK RGGFAKLDQ NHLRFFREL LGENRVLTD 
      PSDCEIYNV DWNRNVRGY SECILKPKT TQEVSQVLS 
      FCNEHNLAV SPQGGNTGL VGGSVPVFD EIVLSLSLM 
      NNIISVDDT SGILVCESG CVLEYLDEQ LAKHRLMMP 
      LDLGAKGSC QIGGNVSTN AGGLRLLRY XNVLGIEAV 
      KANGEILDC LSALKKDNT GFHLKHLFI GSEGSLGVV 
      TKVAIQCPS KPEAVNLAF LGVDSFDKI LTTFKRAKR 
      ELGEILSSF EMIDEQSIN AVIDHLKVK SPISQHPFY 
      ILVETQGSN DGHDQEKIT KFLENLMGD GVAQDGTVT 
      NEPSKMKVI WDLRERIAE AFLHDGYVF KYDITLPLE 
      KFYSVVEVM REKLGSEVV RCCGYGHIG DGNIHFSVC 
      AKEFSQSLL KKIEPFVYE YTSQLKGSI SAEHGIGFR 
      KPKYIHYSK SESAIQLMK DLKKLMDPK GILNPYKVL 
      P
    CDS sequence
    • >TC002688 RA
      atgtttccg aggggtgta aagcagtta ggcaggctg 
      cggccttgc tctctgggg attttaccc tcgtcagtg 
      tgcagaggg ttcgccagc cctacgtta cctgaactc 
      actaaggat cgatatccg cacgtgaag cgaggggga 
      ttcgcgaaa ttggatcag aatcatttg agattcttc 
      agggagctg ctcggggag aaccgagtc ttgactgac 
      cccagcgat tgtgagatc tacaatgta gattggaat 
      aggaatgtt agaggctac agcgaatgc atcttgaag 
      ccgaaaaca acgcaggaa gtatctcaa gtgttgtcc 
      ttttgtaac gagcacaac ctcgctgtc tcgcctcaa 
      gggggtaac accggtctg gtgggtggc tccgtcccc 
      gttttcgac gaaattgtt ctgtccctc agtctcatg 
      aataacatc ataagcgtc gacgacact tccggcatt 
      ctcgtttgt gagagtgga tgcgtgttg gaatattta 
      gacgagcaa ttagccaaa catcgacta atgatgcct 
      ttggatctc ggagcgaag ggttcgtgt cagataggc 
      ggaaatgtt tccactaac gccggaggc ctgcggcta 
      ctcagatac gncaatgtg ctgggaatt gaagcggtg 
      aaagccaac ggtgaaatc ctcgactgt ctcagcgct 
      ttaaagaaa gacaatact ggattccac ttgaagcac 
      cttttcatc ggctctgag ggttctctg ggtgtcgta 
      acaaaagtc gcaatccag tgcccttca aaacctgaa 
      gctgtcaat ttagcattc ttaggcgtc gactccttc 
      gacaaaatc ctaacaact ttcaaacgc gcaaaacgc 
      gaactgggc gaaatttta tcatcgttt gaaatgatc 
      gatgaacag tcgataaat gcagtaatc gaccacttg 
      aaagtcaaa tcgcctatc tcacaacac cctttctac 
      atcctggtc gaaacccag ggcagtaac gatggtcac 
      gaccaggaa aaaatcacc aaatttctg gagaacttg 
      atgggcgat ggggtggcg caagacggg acagtgact 
      aacgaaccg agcaaaatg aaggtgatt tgggatttg 
      cgagagagg atagctgag gcgttttta cacgacggt 
      tatgtcttc aagtatgat ataacgttg ccgcttgag 
      aagttttat tcggttgtt gaggtgatg agggagaag 
      ttgggctcg gaggttgtt aggtgttgc ggttatggg 
      catattgga gatgggaat attcacttt tcggtatgt 
      gcaaaggag ttctcgcaa agtttgttg aaaaaaatt 
      gaaccgttt gtttatgaa tacacgtcg caattgaaa 
      ggaagcatt agcgccgaa catggaata ggttttagg 
      aagccgaag tatatacat tactccaaa agtgagtca 
      gctatacag ctaatgaaa gatctcaag aaattgatg 
      gatccgaag gggatttta aatccttat aaagttttg 
      ccatga
    mRNA sequence
    • >TC002688 RA
      ttcaacaac tgaatattt ttgcaagta ggcgttggg 
      caagtttcg gcgtttctc cctttctac cctcacaaa 
      gtgcacatg tttccgagg ggtgtaaag cagttaggc 
      aggctgcgg ccttgctct ctggggatt ttaccctcg 
      tcagtgtgc agagggttc gccagccct acgttacct 
      gaactcact aaggatcga tatccgcac gtgaagcga 
      gggggattc gcgaaattg gatcagaat catttgaga 
      ttcttcagg gagctgctc ggggagaac cgagtcttg 
      actgacccc agcgattgt gagatctac aatgtagat 
      tggaatagg aatgttaga ggctacagc gaatgcatc 
      ttgaagccg aaaacaacg caggaagta tctcaagtg 
      ttgtccttt tgtaacgag cacaacctc gctgtctcg 
      cctcaaggg ggtaacacc ggtctggtg ggtggctcc 
      gtccccgtt ttcgacgaa attgttctg tccctcagt 
      ctcatgaat aacatcata agcgtcgac gacacttcc 
      ggcattctc gtttgtgag agtggatgc gtgttggaa 
      tatttagac gagcaatta gccaaacat cgactaatg 
      atgcctttg gatctcgga gcgaagggt tcgtgtcag 
      ataggcgga aatgtttcc actaacgcc ggaggcctg 
      cggctactc agatacgnc aatgtgctg ggaattgaa 
      gcggtgaaa gccaacggt gaaatcctc gactgtctc 
      agcgcttta aagaaagac aatactgga ttccacttg 
      aagcacctt ttcatcggc tctgagggt tctctgggt 
      gtcgtaaca aaagtcgca atccagtgc ccttcaaaa 
      cctgaagct gtcaattta gcattctta ggcgtcgac 
      tccttcgac aaaatccta acaactttc aaacgcgca 
      aaacgcgaa ctgggcgaa attttatca tcgtttgaa 
      atgatcgat gaacagtcg ataaatgca gtaatcgac 
      cacttgaaa gtcaaatcg cctatctca caacaccct 
      ttctacatc ctggtcgaa acccagggc agtaacgat 
      ggtcacgac caggaaaaa atcaccaaa tttctggag 
      aacttgatg ggcgatggg gtggcgcaa gacgggaca 
      gtgactaac gaaccgagc aaaatgaag gtgatttgg 
      gatttgcga gagaggata gctgaggcg tttttacac 
      gacggttat gtcttcaag tatgatata acgttgccg 
      cttgagaag ttttattcg gttgttgag gtgatgagg 
      gagaagttg ggctcggag gttgttagg tgttgcggt 
      tatgggcat attggagat gggaatatt cacttttcg 
      gtatgtgca aaggagttc tcgcaaagt ttgttgaaa 
      aaaattgaa ccgtttgtt tatgaatac acgtcgcaa 
      ttgaaagga agcattagc gccgaacat ggaataggt 
      tttaggaag ccgaagtat atacattac tccaaaagt 
      gagtcagct atacagcta atgaaagat ctcaagaaa 
      ttgatggat ccgaagggg attttaaat ccttataaa 
      gttttgcca tgaaaaaat aaagttttc gaacgtttt 
      atgtgtaca tattgcata ctttataca tattgtatt 
      aaatattta cataataaa gaattctat actgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY