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Gene information
General information
TC000024 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16014175..16018397
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000024
    iBeetle screen iB_07020

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber shape irregular - Number of animals showing the phenotype: 4

    egg chamber number decreased - Number of animals showing the phenotype: 4

    follicular epithelium potentially posterior amount decreased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000024 RA
      MKAVSVVCA DNLTGINSG RVSKPKKDI 
ENEEIQMYI SKLKDLVPF MPKNRKLSK 
LEVIQYVID YICDLQSAL ETHPAVGSF 
DAAAALGQK KSQEAVSPR QPLGLRASP 
NTILSPCEA PVLQTPEKL TPSDRQVPC
    CDS sequence
    • >TC000024 RA
      atgaaagct gtcagtgta gtgtgtgca gacaacctc 
accggaatc aactccggg cgagtgtcc aagcccaaa 
aaggatatc gagaacgag gagatccaa atgtacatc 
agcaagctg aaggaccta gtgcccttc atgcccaaa 
aaccggaag ctctccaaa ctggaagtc atccagtac 
gtcatcgac tacatctgc gacctgcag agcgccctg 
gagacgcac cccgccgtg ggcagcttc gacgccgcc 
gccgccctg ggccagaag aagtcgcag gaggcggtc 
agcccccga cagcccctc ggcctgcgc gccagcccc 
aacaccatc ctctccccc tgcgaggcc cccgtgctc 
cagacgcct gaaaaactg acgccgtca gataggcag 
gtcccttgc taa
    mRNA sequence
    • >TC000024 RA
      gtcaaacaa gtatttatt ggcgaatta gtatcacct 
ctaggtgag ccgagaagt gtggcgcct tgtccggcg 
tgaaaaaac agttttgca ttagctagg tgggttgta 
atttgcatt acaaagtgt acgaactgt ttcgacccg 
acacaacca ggttagtta cctgtagta tcattttca 
aatttcaag ctacaatta agcgaccat caatcatcc 
gcattaagc cgaattcag cacaaacca ttcaacact 
cgccctttc cgctcaaga gtcgaaaac taatcttcc 
tcaagcaag ttcacggaa atgctattg gtaggacgt 
ttgcgccgt gttgaacta ggcgcctgc aacactacc 
tgaacctga agtgctctc attggccgg gctacgccc 
cacacagcc tggcgcctc gtcccattg gccgccccc 
cctccacca aacctataa aaacctagg caggcctat 
tgcctcctc attcgacca ttcgttcgc tcgtagtgc 
gtatcagtg ttcagtgta cgaaatgaa agctgtcag 
tgtagtgtg tgcagacaa cctcaccgg aatcaactc 
cgggcgagt gtccaagcc caaaaagga tatcgagaa 
cgaggagat ccaaatgta catcagcaa gctgaagga 
cctagtgcc cttcatgcc caaaaaccg gaagctctc 
caaactgga agtcatcca gtacgtcat cgactacat 
ctgcgacct gcagagcgc cctggagac gcaccccgc 
cgtgggcag cttcgacgc cgccgccgc cctgggcca 
gaagaagtc gcaggaggc ggtcagccc ccgacagcc 
cctcggcct gcgcgccag ccccaacac catcctctc 
cccctgcga ggcccccgt gctccagac gcctgaaaa 
actgacgcc gtcagatag gcaggtccc ttgctaagt 
gggatcttc gaacacagt gatcttgaa tatcatcct 
catctcatt ccgaaagaa tcagcctcg gactgcgag 
gctctattt ttggataaa tcgcctcat tttagttag 
aaagaacga ctgaagcca ttatttata ttgcgagtg 
gagaggaac tacagtgtg tgcgttgag gagaaagtg 
aaatatgtg aagcactta gtgatgtac ctgtttatg 
ttgcaattg ttatgtaga ttttatttt attgttttg 
tacatatgt taggcgagt ttattgtac ttttccatt 
tttttattt ggtagtaat gcaaaattc attcgtgaa 
attttgact gtagcttta tcacagatt ttaattgat 
tgattttgt gacttttca ttctgtgat ttttagcaa 
gtaatagtg tacaaagtg taatctctg cgcgagaat 
tgttggggt cacggtttc gactgtgat tttgactct 
gtacatttt tgtttctag aacttattg taaatagtt 
acctatata gcaactgaa tgttgtttg aatttttat 
tatatgaag atatttcaa ataaaatga ttttagaag 
g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY