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Gene information
General information
TC011078 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5802876..5860227
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011078
    iBeetle screen iB_07019

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Decreased fluorescence included
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    labium size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011078 RA
      MGSSMLWMA ILGAILVWS APGAPSRRG QAYQISPKP 
      CWVDGQEGT CMFVYECIK SEGYHIGMC VDTFMFGSC 
      CAHNATDNN VVPIQHDQP AVLYTPPSQ HSHTTNKPI 
      SHSRPRPTS HSHLSQSTK APNRPVTPP PAHTLAASM 
      NVRPNLSSR NPTQPPRHT DALSGANSI AIGVYTKPG 
      WQSTTEPGF ITSHNQNSI QTGSKPQWQ VTTEPAFVT 
      RVKAKPSSF KPKPKPTKK PIQNTTKVG SKGTTKATT 
      KRPPTSASP VVNTEATTV QKVSTAPDL MQTSTIGSV 
      VSAPISARV ECGVPKMTT QPQSKIVGG KNAPFGRWP 
      WQVSVRRTS FFGFSSTHR CGGAILNEN WIATAGHCV 
      DE
    • >TC011078 RB
      MLWMAILGA ILVWSAPGA PSRRGQAYQ ISPKPCWVD 
      GQEGTCMFV YECIKSEGY HIGMCVDTF MFGSCCAHN 
      ATDNNVVPI QHDQPAVLY TPPSQHSHT TNKPISHSR 
      PRPTSHSHL SQSTKAPNR PVTPPPAHT LAASMNVRP 
      NLSSRNPTQ PPRHTDALS GANSIAIGV YTKPGWQST 
      TEPGFITSH NQNSIQTGS KPQWQVTTE PAFVTRVKA 
      KPSSFKPKP KPTKKPIQN TTKVGSKGT TKATTKRPP 
      TSASPVVNT EATTVQKVS TAPDLMQTS TIGSVVSAP 
      ISARVECGV PKMTTQPQS KIVGGKNAP FGRWPWQVS 
      VRRTSFFGF SSTHRCGGA ILNENWIAT AGHCVDE
    CDS sequence
    • >TC011078 RA
      atgggatca agcatgctc tggatggcc attcttggc 
      gccatattg gtgtggtca gctccaggt gctccatct 
      cggagaggc caagcttat cagataagt ccgaaacct 
      tgctgggtc gacggccaa gaaggcacg tgcatgttc 
      gtgtacgag tgcatcaaa tccgagggg taccacatc 
      ggcatgtgc gtggacacc tttatgttt ggttcctgc 
      tgtgcacac aacgctacc gacaacaac gtcgtaccc 
      atccaacac gaccaaccg gccgtgctc tacactcct 
      cctagtcaa catagtcat accactaac aagccaatt 
      agtcatagt aggcccagg ccgacaagt cattcgcac 
      ctaagtcag tcaacaaaa gcgccaaat cgtcccgta 
      actccgcct ccagcccat actctggcg gcctctatg 
      aacgtgagg cccaacttg tcgtcgagg aaccccaca 
      caaccaccc cgtcacacc gacgccctc tcaggggct 
      aatagtata gcgataggg gtttatacg aagccggga 
      tggcaatcg acgacagaa ccgggtttt atcacgtct 
      cacaaccag aattccatt cagacgggc tccaaaccg 
      cagtggcag gtcactaca gaaccggct tttgtgacg 
      agagttaaa gcgaaacct agtagtttt aagccgaag 
      ccgaaaccc accaagaaa ccgatacag aatacgacg 
      aaggtgggc agcaagggg accacgaaa gctaccaca 
      aaaaggccg ccaacttca gcgagtcct gtcgtcaac 
      actgaagcc accactgtg caaaaagtt tcaacagct 
      cctgatttg atgcaaacg tccacgatt ggaagtgtt 
      gtctcagct ccaatttcg gcgagagtt gaatgtggt 
      gtacccaag atgacgacc caaccccag tcgaaaatc 
      gtggggggt aagaacgcc ccttttggc cggtggccc 
      tggcaggtt tcagtgaga aggacctcc tttttcggc 
      ttctccagc acgcatagg tgcggaggt gcgatcctt 
      aacgagaat tggatcgca acagctgga cattgcgtc 
      gatgagtaa
    • >TC011078 RB
      atgctctgg atggccatt cttggcgcc atattggtg 
      tggtcagct ccaggtgct ccatctcgg agaggccaa 
      gcttatcag ataagtccg aaaccttgc tgggtcgac 
      ggccaagaa ggcacgtgc atgttcgtg tacgagtgc 
      atcaaatcc gaggggtac cacatcggc atgtgcgtg 
      gacaccttt atgtttggt tcctgctgt gcacacaac 
      gctaccgac aacaacgtc gtacccatc caacacgac 
      caaccggcc gtgctctac actcctcct agtcaacat 
      agtcatacc actaacaag ccaattagt catagtagg 
      cccaggccg acaagtcat tcgcaccta agtcagtca 
      acaaaagcg ccaaatcgt cccgtaact ccgcctcca 
      gcccatact ctggcggcc tctatgaac gtgaggccc 
      aacttgtcg tcgaggaac cccacacaa ccaccccgt 
      cacaccgac gccctctca ggggctaat agtatagcg 
      ataggggtt tatacgaag ccgggatgg caatcgacg 
      acagaaccg ggttttatc acgtctcac aaccagaat 
      tccattcag acgggctcc aaaccgcag tggcaggtc 
      actacagaa ccggctttt gtgacgaga gttaaagcg 
      aaacctagt agttttaag ccgaagccg aaacccacc 
      aagaaaccg atacagaat acgacgaag gtgggcagc 
      aaggggacc acgaaagct accacaaaa aggccgcca 
      acttcagcg agtcctgtc gtcaacact gaagccacc 
      actgtgcaa aaagtttca acagctcct gatttgatg 
      caaacgtcc acgattgga agtgttgtc tcagctcca 
      atttcggcg agagttgaa tgtggtgta cccaagatg 
      acgacccaa ccccagtcg aaaatcgtg gggggtaag 
      aacgcccct tttggccgg tggccctgg caggtttca 
      gtgagaagg acctccttt ttcggcttc tccagcacg 
      cataggtgc ggaggtgcg atccttaac gagaattgg 
      atcgcaaca gctggacat tgcgtcgat gagtaa
    mRNA sequence
    • >TC011078 RA
      atgcaggtg caggtggat ggtggagaa atggtgcgg 
      gtgtgaacg actcaaatc ggtgtagct gtcagtgcg 
      agataataa attgctcaa gagctcctc atgaatgaa 
      acagcagtg gcggcgcgt cttagcatt tgatgcaac 
      aataaaatc gtttacaac aataataaa gttaagtga 
      tgtttaacg tgcgattcg ctctctaac tgttactgc 
      gcgggtgac aatttgtgc tttgtttga cagttaatt 
      atagtgcga atggattgt cgtggagtt acattgtgg 
      tagatagag gacttaatg ggatcaagc atgctctgg 
      atggccatt cttggcgcc atattggtg tggtcagct 
      ccaggtgct ccatctcgg agaggccaa gcttatcag 
      ataagtccg aaaccttgc tgggtcgac ggccaagaa 
      ggcacgtgc atgttcgtg tacgagtgc atcaaatcc 
      gaggggtac cacatcggc atgtgcgtg gacaccttt 
      atgtttggt tcctgctgt gcacacaac gctaccgac 
      aacaacgtc gtacccatc caacacgac caaccggcc 
      gtgctctac actcctcct agtcaacat agtcatacc 
      actaacaag ccaattagt catagtagg cccaggccg 
      acaagtcat tcgcaccta agtcagtca acaaaagcg 
      ccaaatcgt cccgtaact ccgcctcca gcccatact 
      ctggcggcc tctatgaac gtgaggccc aacttgtcg 
      tcgaggaac cccacacaa ccaccccgt cacaccgac 
      gccctctca ggggctaat agtatagcg ataggggtt 
      tatacgaag ccgggatgg caatcgacg acagaaccg 
      ggttttatc acgtctcac aaccagaat tccattcag 
      acgggctcc aaaccgcag tggcaggtc actacagaa 
      ccggctttt gtgacgaga gttaaagcg aaacctagt 
      agttttaag ccgaagccg aaacccacc aagaaaccg 
      atacagaat acgacgaag gtgggcagc aaggggacc 
      acgaaagct accacaaaa aggccgcca acttcagcg 
      agtcctgtc gtcaacact gaagccacc actgtgcaa 
      aaagtttca acagctcct gatttgatg caaacgtcc 
      acgattgga agtgttgtc tcagctcca atttcggcg 
      agagttgaa tgtggtgta cccaagatg acgacccaa 
      ccccagtcg aaaatcgtg gggggtaag aacgcccct 
      tttggccgg tggccctgg caggtttca gtgagaagg 
      acctccttt ttcggcttc tccagcacg cataggtgc 
      ggaggtgcg atccttaac gagaattgg atcgcaaca 
      gctggacat tgcgtcgat gagtaagtt attaaattg 
      agttgaatt ta
    • >TC011078 RB
      ttggagaac tgttgagtg gtgtcagtg tcttctcag 
      atggtcacc aactcacat gacagctta agtgctatg 
      ctcaagacg ttgcctttt taattcgtt tcaaacgcg 
      gagtggagt taattaaaa tgtgcgacc tggtggagt 
      gcaaataag ggaggaatg cgagacccc aaagggagg 
      gaaaagggt ttatgaagg catcggatc aagcatgct 
      ctggatggc cattcttgg cgccatatt ggtgtggtc 
      agctccagg tgctccatc tcggagagg ccaagctta 
      tcagataag tccgaaacc ttgctgggt cgacggcca 
      agaaggcac gtgcatgtt cgtgtacga gtgcatcaa 
      atccgaggg gtaccacat cggcatgtg cgtggacac 
      ctttatgtt tggttcctg ctgtgcaca caacgctac 
      cgacaacaa cgtcgtacc catccaaca cgaccaacc 
      ggccgtgct ctacactcc tcctagtca acatagtca 
      taccactaa caagccaat tagtcatag taggcccag 
      gccgacaag tcattcgca cctaagtca gtcaacaaa 
      agcgccaaa tcgtcccgt aactccgcc tccagccca 
      tactctggc ggcctctat gaacgtgag gcccaactt 
      gtcgtcgag gaaccccac acaaccacc ccgtcacac 
      cgacgccct ctcaggggc taatagtat agcgatagg 
      ggtttatac gaagccggg atggcaatc gacgacaga 
      accgggttt tatcacgtc tcacaacca gaattccat 
      tcagacggg ctccaaacc gcagtggca ggtcactac 
      agaaccggc ttttgtgac gagagttaa agcgaaacc 
      tagtagttt taagccgaa gccgaaacc caccaagaa 
      accgataca gaatacgac gaaggtggg cagcaaggg 
      gaccacgaa agctaccac aaaaaggcc gccaacttc 
      agcgagtcc tgtcgtcaa cactgaagc caccactgt 
      gcaaaaagt ttcaacagc tcctgattt gatgcaaac 
      gtccacgat tggaagtgt tgtctcagc tccaatttc 
      ggcgagagt tgaatgtgg tgtacccaa gatgacgac 
      ccaacccca gtcgaaaat cgtgggggg taagaacgc 
      cccttttgg ccggtggcc ctggcaggt ttcagtgag 
      aaggacctc ctttttcgg cttctccag cacgcatag 
      gtgcggagg tgcgatcct taacgagaa ttggatcgc 
      aacagctgg acattgcgt cgatgagta agttattaa 
      attgagttg aattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY