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Gene information
General information
TC011399 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 876200..884805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011399
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07017

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 12.5% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011399 RA
      MTDEEGPKY KDGDIVWVK LGPCWWPGE VTEDAPEDF 
      TAKKPPLAI VKFFNEATY EFVKSHNHI YFYNCERKN 
      EFIKKGMDS FRANNPHME KFPGDVSLA EIRTDGNPN 
      ILSDPIFAP QKKSNIIAE VFGSPAPKK TPKDGRGRS 
      KNSSKKPKV NITHRRFLG LDDYESHIR VQFPGKDVE 
      NSSGDEEIV KLNAEPEKT FNCSSCSFQ TNRIDVMLF 
      HVQCHIKGI ASPGPVKKK TAPKKKKSK IIKHITSDD 
      DHTNSSSAF LDTDTDEEE EEKPKPKRR RRTVSPKKK 
      PKRKEEPTV DIRTDLLAE WSDSEENEA EEAETSKEE 
      NVSNEETKN DAKDLNKSC FDFDEEEEE NEDMGETAT 
      GRKIPRVIP PKSSELEET GSESTEATN EEFKDLMEA 
      TSVPELPEV PKPQNNLKT EVATKLTNP KKRFVKSFE 
      DFELLQNEQ RKAQEEEEK NKGKEEHKT AESETVEEK 
      AESNEESSK DAEKVETIE EKTEEKEKI EEEKIASNL 
      QISKLKCQI MSKLGDEEQ ELSKKVAKT SHRSPRSKI 
      LESYRKDKR KSVEDEEEA KKVEPVIEE KEEEEKKPV 
      EEPAEEEKK PDKEIDDYL NEFLFDFPK KEEEEKEKI 
      EPEESSNEV RIKSDSGDM DIAIKTNLS NFNKDEEEM 
      SALSTLAMV SQTVEEEEK EEIKLPFVE KPETEMVPI 
      DGNLTKVSE KDLADGQVE ITATCKKPV TLTSFSMDF 
      PDCNNSNSE STTSEASES KPVEEPPKN DDLMDLEEH 
      IPSQIVDKK PKEEEEPVA TSKLLERLT EKPKKVPKD 
      NSSSSTKVT KKFVIKSPP TTSSNNKPV ILSEKIIKP 
      VITTASVSV QKVTSKRNL HDPDEIDAF IIQKGNKTQ 
      EDEESDETP VKKLKKSAV SSTSVKKRG KAKILQQTI 
      ITPAGEIIQ PGVPTTDDN NVFDINSMP IVLSDQILT 
      PESIENMPI YLSEAPTET PKIVKKPPQ TTQMRVLNK 
      SIATPTPGT KLIKTPKIL GSIGGTTTK LIKQNSFSP 
      KQKFVIVTQ PPTTGTTTS KYTVGKKII KSAATLTKV 
      QNIAPEPSG NKIMILTDQ QGQQQRVLL TPAQQKMMG 
      YQNQGKVKT VMKGNIIQK AKDGSVAGS SGLVTKTVT 
      PSRINSLIS SNRPITGRK IQMQSQKVP IKPPQKTIL 
      IKNQHGQTV RKIQGTDDA LLDKQVAEQ IEAIKASGV 
      LQQKQNTTA TPKTVTTTP TVRRSYVKK IESSPKKTE 
      PAVPPLAPI SPKKPESES KDNKPDRAL HQLVIQDAM 
      GNQTTITEG QILALPSET IDGQPQSYM LVTLDESGN 
      LTPLNNEAL LSLDPNLGL GGDLGNTVL QIDQNNATN 
      TQTETVEAP PKSTEEEGA ARAEEEKAQ QAGDGQQLI 
      VTGDPIAAQ KFLESLTEG NTDLANILA SAEGSSILI 
      HADGQQILI NTDSDNQML LSVNTDNLN MAESSEGGG 
      NPIFATQPS KNQDILAAA LADTDVFQG EQNNGQKLS 
      PNSSLYPMN VGNVLETSL TLNSPIMTP LEVPSTNSK 
      KIDESDILA PVPKSVDLP ITITDPNIS QTVAQQQVA 
      LPGNLELAL PISESAIVT CSDMSYYSL SDRSMPILT 
      EDDDKAKGE DEGLCTLGG EMCSSLSEP PPDMFDLTA 
      VLSSQKEKD ESNPGSVTE TSSPNTLNE DSCEIPVQP 
      EIVTKLDEE KEANSTEVV
    CDS sequence
    • >TC011399 RA
      atgaccgac gaagaaggc cccaagtac aaggacggt 
      gacattgtt tgggtgaaa ttggggccc tgttggtgg 
      ccgggcgaa gtcaccgaa gacgccccc gaagacttc 
      accgccaaa aagccacca ttagccatc gtcaagttc 
      ttcaacgag gccacttat gaatttgtc aagagccac 
      aaccatatt tatttttac aattgcgag cgcaaaaat 
      gagtttatc aaaaaagga atggattcc ttccgtgcc 
      aacaacccc cacatggag aaatttcca ggcgacgtt 
      agtttggcc gaaatacgc acagatggc aaccctaac 
      attctctca gacccgata ttcgccccc caaaagaaa 
      agtaatatc attgcagag gtgtttggc agccctgca 
      cccaaaaaa accccaaaa gacggccga ggacgctcg 
      aaaaattcg tccaaaaag cccaaagtt aacataacc 
      catcgccga tttctcggt ttagacgat tacgagtca 
      catattcgt gtccaattt ccgggaaaa gacgtcgaa 
      aattccagt ggtgatgaa gaaattgtt aaattgaac 
      gctgaaccc gaaaaaacg ttcaattgt tcgtcttgt 
      agttttcaa acgaatcga attgatgtg atgttgttt 
      cacgttcag tgtcatatt aaaggaatc gcatcacct 
      gggccggtt aagaagaaa actgcacca aaaaagaag 
      aaatcgaag attattaaa catataact tccgatgac 
      gatcacacg aattcaagt tctgccttc cttgataca 
      gacactgac gaggaagaa gaagaaaaa ccgaaaccg 
      aaacgaaga agacgaacg gtttcaccg aagaagaaa 
      cccaaacga aaagaagaa ccaactgtt gatattcgc 
      accgacctt ttggccgaa tggtccgat agtgaagaa 
      aatgaagca gaagaagcc gaaactagc aaagaagaa 
      aatgtttcg aacgaagaa acgaagaat gatgctaaa 
      gatttgaac aaatcgtgc ttcgatttc gacgaagaa 
      gaggaagaa aatgaagat atgggtgaa acagcaact 
      ggacgtaaa atcccacgt gtaattccc ccaaaatcg 
      agcgaactt gaagaaacc ggaagcgaa tcaactgaa 
      gcaacaaac gaagaattc aaagatctg atggaagca 
      acatcggtt cccgaacta ccagaagta ccaaaacca 
      cagaataat ctcaaaacg gaagttgca acgaaatta 
      acaaacccg aagaaacgt ttcgttaaa tcgtttgaa 
      gatttcgaa ttgttgcaa aacgagcaa cgcaaagca 
      caagaagaa gaagaaaag aataaggga aaagaagaa 
      cacaaaacg gccgagtcc gaaactgtt gaagaaaag 
      gccgaatcg aatgaagaa agttcgaaa gatgccgaa 
      aaagttgaa acaattgaa gaaaaaact gaagaaaag 
      gaaaaaatt gaggaggaa aaaatcgcc tcaaatcta 
      caaatatca aaattaaaa tgtcaaatt atgtcgaaa 
      cttggcgat gaagaacaa gaactgtcg aagaaagtg 
      gcgaaaact tcgcacaga tcgccacga agtaaaatt 
      ctggaatcg tatcgaaaa gacaagagg aaaagtgtc 
      gaagatgaa gaagaagcg aagaaagtc gaaccggtg 
      attgaagaa aaagaagaa gaagagaag aaaccggtc 
      gaggaaccg gctgaagaa gagaagaaa ccggacaag 
      gaaatcgac gattatctc aatgagttc cttttcgat 
      tttccgaag aaggaagaa gaagaaaag gagaagatc 
      gaacctgaa gaaagctcg aacgaggtt cggatcaag 
      tcggattcg ggcgacatg gatattgcg attaaaacc 
      aatttgagt aattttaac aaagatgag gaggaaatg 
      tcagcgctg agtacgcta gcaatggtt tcgcaaacg 
      gtcgaagaa gaagaaaag gaagaaatc aagttaccg 
      tttgttgaa aaacccgaa actgagatg gtaccaatc 
      gacggtaac ttgaccaaa gtatcggag aaggattta 
      gccgatggt caagttgaa atcaccgca acttgcaaa 
      aaaccggtc actttgact agtttttcc atggatttc 
      cccgactgt aataatagt aatagtgaa agtacaacg 
      agcgaagcg agtgagtca aaaccagtc gaagaaccg 
      ccgaaaaat gacgatcta atggatttg gaagaacac 
      ataccgtca caaattgtc gataaaaaa ccgaaagaa 
      gaagaagaa ccagtggcg acgtcgaaa ctactcgaa 
      cgtctgacc gaaaaaccg aaaaaagtg cctaaagat 
      aatagtagt agtagtaca aaagtgaca aagaaattc 
      gtaatcaag tcgccacca acgactagt tccaacaac 
      aaaccggtt attttatcc gagaagatt attaaaccg 
      gttataacc actgcttcg gtttcggtt cagaaagtc 
      acttcgaag cgaaatctc cacgatccg gacgaaatc 
      gacgctttt attatccag aaaggtaac aaaacacaa 
      gaagatgaa gaaagtgac gaaacaccg gttaaaaaa 
      ctgaagaaa agtgccgtt tcgtcgact tcggtgaag 
      aaacgcggg aaagcgaag atcctgcaa cagactata 
      atcacacca gctggggaa atcatccaa ccaggtgtg 
      ccaaccact gacgacaac aacgtcttc gacatcaat 
      tcaatgcca attgttctc tccgatcaa atcctaaca 
      cctgaatcc attgaaaat atgccaatt tatttatcc 
      gaagcgcca actgagacg ccaaaaatc gtcaaaaaa 
      ccaccacaa acaactcaa atgcgtgtt ttaaataaa 
      tcgattgca acgccaact cccggcact aagttgatt 
      aaaacaccg aaaattttg ggctcgatt ggtggcacc 
      accaccaaa ttgattaaa caaaattcc tttagtcct 
      aaacaaaaa ttcgtgatt gtaacacaa ccaccaacc 
      actggcacg actactagt aaatacacc gtgggtaaa 
      aaaataatc aagagtgcg gcaacgttg actaaagta 
      caaaatatt gcaccggaa ccgagtggt aataaaata 
      atgattttg actgatcaa caaggacaa caacagaga 
      gtgcttctg acgccagcg caacaaaaa atgatgggg 
      tatcagaat caggggaag gtcaagact gtgatgaaa 
      ggtaatata atccagaag gcgaaggac ggttcagtg 
      gcgggctcc agcggccta gtcacaaaa acagtgaca 
      ccaagtcgc atcaattca ttgatctcg tcaaatcgg 
      ccaatcacc ggccgtaaa atccaaatg caaagtcaa 
      aaagtacca atcaaacca ccgcaaaaa accatcctc 
      attaaaaac caacacggt caaaccgtt cgtaaaatc 
      caaggcacc gacgacgct cttttagac aaacaagtc 
      gccgaacaa atcgaagcg atcaaagca agcggcgta 
      ttacaacaa aaacaaaac acaacagca acaccgaaa 
      acagtaaca accacacca accgtgcga cgttcctac 
      gtcaaaaaa atcgaaagt agtccgaaa aaaaccgaa 
      cctgctgta ccaccactc gcgccaatt tcgccgaaa 
      aaacccgaa agtgaatcg aaagacaat aaaccggac 
      cgagcccta catcagttg gtaatccag gacgccatg 
      ggcaaccaa actacgatc acagaaggc cagattttg 
      gcattgccg agtgagaca atcgatggg cagccacag 
      tcctacatg ttggtaaca ttggacgaa agtggcaac 
      ttgaccccg cttaataac gaagccctg ttgtcactt 
      gatccgaat ctgggattg gggggcgat ttaggcaac 
      actgtgctg caaattgat caaaataac gccacgaac 
      acacaaact gagacggtg gaagcgcca ccgaaaagt 
      accgaggaa gaaggcgct gcacgagcc gaagaagaa 
      aaggcgcaa caagcaggc gacggccag cagttgatt 
      gtgacaggt gatccaata gccgcccaa aaattctta 
      gaatcacta actgaaggc aacactgac ttggccaat 
      atcctggca agtgccgaa ggctcaagc attttaata 
      catgctgac ggtcaacaa atcctaatc aacacggat 
      tccgacaat caaatgtta ctctccgtc aacacggat 
      aatttaaac atggcggaa agtagcgag ggtgggggt 
      aatccaatc ttcgccacc caaccgtcg aaaaatcaa 
      gacatcctt gccgccgcc ttggccgac actgatgtc 
      tttcaaggc gagcaaaac aacggccaa aaactgagc 
      cccaacagt agtttatat ccaatgaat gttggcaat 
      gtcctcgag accagcctg accttgaat tcaccaatc 
      atgacaccg ctggaagtg ccgagcacc aatagcaag 
      aaaatcgac gagagcgat attctggcg ccggtaccg 
      aaatcagtc gatctacca atcacaatc accgatccg 
      aatatctcg caaacggtt gcgcagcaa caagtcgcg 
      ttgccgggc aacttggag ctggcgttg ccaatcagc 
      gagtcggcc attgtcacg tgctccgac atgtcttat 
      tattcgctg tcggaccga agtatgccg atcttgacc 
      gaggatgac gacaaggcg aagggcgag gatgagggg 
      ttgtgcaca ttggggggc gagatgtgc agttcgttg 
      agtgaaccc ccgcctgat atgttcgat ttaacggcc 
      gttttgtcc tctcagaag gaaaaggac gagtcgaat 
      ccgggttcg gtcactgag accagtagt ccgaatacg 
      ttgaatgag gattcgtgt gaaataccg gtacagccg 
      gagattgtc acgaagttg gacgaggag aaagaggcg 
      aattcgact gaggttgtg taa
    mRNA sequence
    • >TC011399 RA
      gccaaccga aacaaccca agccgagtg gtgtcaatt 
      cgtggggta attccccgt ttgtggctt agttggggc 
      tttggccgt ctcccggag cccctttcg tccatttga 
      cgtcacaaa tggccccaa aagtgtgaa aaatcgtgc 
      aaaaagcgc aaaaactgg tgctttttt gtccgttgt 
      gttggcgtt cggcgacgc caagccgca tggtaccat 
      gggataatc atgtacgtc tgtgacaag tgaaacaat 
      cgagtttga aggcgagaa ttaaatgac acgggcaaa 
      atgaccgac gaagaaggc cccaagtac aaggacggt 
      gacattgtt tgggtgaaa ttggggccc tgttggtgg 
      ccgggcgaa gtcaccgaa gacgccccc gaagacttc 
      accgccaaa aagccacca ttagccatc gtcaagttc 
      ttcaacgag gccacttat gaatttgtc aagagccac 
      aaccatatt tatttttac aattgcgag cgcaaaaat 
      gagtttatc aaaaaagga atggattcc ttccgtgcc 
      aacaacccc cacatggag aaatttcca ggcgacgtt 
      agtttggcc gaaatacgc acagatggc aaccctaac 
      attctctca gacccgata ttcgccccc caaaagaaa 
      agtaatatc attgcagag gtgtttggc agccctgca 
      cccaaaaaa accccaaaa gacggccga ggacgctcg 
      aaaaattcg tccaaaaag cccaaagtt aacataacc 
      catcgccga tttctcggt ttagacgat tacgagtca 
      catattcgt gtccaattt ccgggaaaa gacgtcgaa 
      aattccagt ggtgatgaa gaaattgtt aaattgaac 
      gctgaaccc gaaaaaacg ttcaattgt tcgtcttgt 
      agttttcaa acgaatcga attgatgtg atgttgttt 
      cacgttcag tgtcatatt aaaggaatc gcatcacct 
      gggccggtt aagaagaaa actgcacca aaaaagaag 
      aaatcgaag attattaaa catataact tccgatgac 
      gatcacacg aattcaagt tctgccttc cttgataca 
      gacactgac gaggaagaa gaagaaaaa ccgaaaccg 
      aaacgaaga agacgaacg gtttcaccg aagaagaaa 
      cccaaacga aaagaagaa ccaactgtt gatattcgc 
      accgacctt ttggccgaa tggtccgat agtgaagaa 
      aatgaagca gaagaagcc gaaactagc aaagaagaa 
      aatgtttcg aacgaagaa acgaagaat gatgctaaa 
      gatttgaac aaatcgtgc ttcgatttc gacgaagaa 
      gaggaagaa aatgaagat atgggtgaa acagcaact 
      ggacgtaaa atcccacgt gtaattccc ccaaaatcg 
      agcgaactt gaagaaacc ggaagcgaa tcaactgaa 
      gcaacaaac gaagaattc aaagatctg atggaagca 
      acatcggtt cccgaacta ccagaagta ccaaaacca 
      cagaataat ctcaaaacg gaagttgca acgaaatta 
      acaaacccg aagaaacgt ttcgttaaa tcgtttgaa 
      gatttcgaa ttgttgcaa aacgagcaa cgcaaagca 
      caagaagaa gaagaaaag aataaggga aaagaagaa 
      cacaaaacg gccgagtcc gaaactgtt gaagaaaag 
      gccgaatcg aatgaagaa agttcgaaa gatgccgaa 
      aaagttgaa acaattgaa gaaaaaact gaagaaaag 
      gaaaaaatt gaggaggaa aaaatcgcc tcaaatcta 
      caaatatca aaattaaaa tgtcaaatt atgtcgaaa 
      cttggcgat gaagaacaa gaactgtcg aagaaagtg 
      gcgaaaact tcgcacaga tcgccacga agtaaaatt 
      ctggaatcg tatcgaaaa gacaagagg aaaagtgtc 
      gaagatgaa gaagaagcg aagaaagtc gaaccggtg 
      attgaagaa aaagaagaa gaagagaag aaaccggtc 
      gaggaaccg gctgaagaa gagaagaaa ccggacaag 
      gaaatcgac gattatctc aatgagttc cttttcgat 
      tttccgaag aaggaagaa gaagaaaag gagaagatc 
      gaacctgaa gaaagctcg aacgaggtt cggatcaag 
      tcggattcg ggcgacatg gatattgcg attaaaacc 
      aatttgagt aattttaac aaagatgag gaggaaatg 
      tcagcgctg agtacgcta gcaatggtt tcgcaaacg 
      gtcgaagaa gaagaaaag gaagaaatc aagttaccg 
      tttgttgaa aaacccgaa actgagatg gtaccaatc 
      gacggtaac ttgaccaaa gtatcggag aaggattta 
      gccgatggt caagttgaa atcaccgca acttgcaaa 
      aaaccggtc actttgact agtttttcc atggatttc 
      cccgactgt aataatagt aatagtgaa agtacaacg 
      agcgaagcg agtgagtca aaaccagtc gaagaaccg 
      ccgaaaaat gacgatcta atggatttg gaagaacac 
      ataccgtca caaattgtc gataaaaaa ccgaaagaa 
      gaagaagaa ccagtggcg acgtcgaaa ctactcgaa 
      cgtctgacc gaaaaaccg aaaaaagtg cctaaagat 
      aatagtagt agtagtaca aaagtgaca aagaaattc 
      gtaatcaag tcgccacca acgactagt tccaacaac 
      aaaccggtt attttatcc gagaagatt attaaaccg 
      gttataacc actgcttcg gtttcggtt cagaaagtc 
      acttcgaag cgaaatctc cacgatccg gacgaaatc 
      gacgctttt attatccag aaaggtaac aaaacacaa 
      gaagatgaa gaaagtgac gaaacaccg gttaaaaaa 
      ctgaagaaa agtgccgtt tcgtcgact tcggtgaag 
      aaacgcggg aaagcgaag atcctgcaa cagactata 
      atcacacca gctggggaa atcatccaa ccaggtgtg 
      ccaaccact gacgacaac aacgtcttc gacatcaat 
      tcaatgcca attgttctc tccgatcaa atcctaaca 
      cctgaatcc attgaaaat atgccaatt tatttatcc 
      gaagcgcca actgagacg ccaaaaatc gtcaaaaaa 
      ccaccacaa acaactcaa atgcgtgtt ttaaataaa 
      tcgattgca acgccaact cccggcact aagttgatt 
      aaaacaccg aaaattttg ggctcgatt ggtggcacc 
      accaccaaa ttgattaaa caaaattcc tttagtcct 
      aaacaaaaa ttcgtgatt gtaacacaa ccaccaacc 
      actggcacg actactagt aaatacacc gtgggtaaa 
      aaaataatc aagagtgcg gcaacgttg actaaagta 
      caaaatatt gcaccggaa ccgagtggt aataaaata 
      atgattttg actgatcaa caaggacaa caacagaga 
      gtgcttctg acgccagcg caacaaaaa atgatgggg 
      tatcagaat caggggaag gtcaagact gtgatgaaa 
      ggtaatata atccagaag gcgaaggac ggttcagtg 
      gcgggctcc agcggccta gtcacaaaa acagtgaca 
      ccaagtcgc atcaattca ttgatctcg tcaaatcgg 
      ccaatcacc ggccgtaaa atccaaatg caaagtcaa 
      aaagtacca atcaaacca ccgcaaaaa accatcctc 
      attaaaaac caacacggt caaaccgtt cgtaaaatc 
      caaggcacc gacgacgct cttttagac aaacaagtc 
      gccgaacaa atcgaagcg atcaaagca agcggcgta 
      ttacaacaa aaacaaaac acaacagca acaccgaaa 
      acagtaaca accacacca accgtgcga cgttcctac 
      gtcaaaaaa atcgaaagt agtccgaaa aaaaccgaa 
      cctgctgta ccaccactc gcgccaatt tcgccgaaa 
      aaacccgaa agtgaatcg aaagacaat aaaccggac 
      cgagcccta catcagttg gtaatccag gacgccatg 
      ggcaaccaa actacgatc acagaaggc cagattttg 
      gcattgccg agtgagaca atcgatggg cagccacag 
      tcctacatg ttggtaaca ttggacgaa agtggcaac 
      ttgaccccg cttaataac gaagccctg ttgtcactt 
      gatccgaat ctgggattg gggggcgat ttaggcaac 
      actgtgctg caaattgat caaaataac gccacgaac 
      acacaaact gagacggtg gaagcgcca ccgaaaagt 
      accgaggaa gaaggcgct gcacgagcc gaagaagaa 
      aaggcgcaa caagcaggc gacggccag cagttgatt 
      gtgacaggt gatccaata gccgcccaa aaattctta 
      gaatcacta actgaaggc aacactgac ttggccaat 
      atcctggca agtgccgaa ggctcaagc attttaata 
      catgctgac ggtcaacaa atcctaatc aacacggat 
      tccgacaat caaatgtta ctctccgtc aacacggat 
      aatttaaac atggcggaa agtagcgag ggtgggggt 
      aatccaatc ttcgccacc caaccgtcg aaaaatcaa 
      gacatcctt gccgccgcc ttggccgac actgatgtc 
      tttcaaggc gagcaaaac aacggccaa aaactgagc 
      cccaacagt agtttatat ccaatgaat gttggcaat 
      gtcctcgag accagcctg accttgaat tcaccaatc 
      atgacaccg ctggaagtg ccgagcacc aatagcaag 
      aaaatcgac gagagcgat attctggcg ccggtaccg 
      aaatcagtc gatctacca atcacaatc accgatccg 
      aatatctcg caaacggtt gcgcagcaa caagtcgcg 
      ttgccgggc aacttggag ctggcgttg ccaatcagc 
      gagtcggcc attgtcacg tgctccgac atgtcttat 
      tattcgctg tcggaccga agtatgccg atcttgacc 
      gaggatgac gacaaggcg aagggcgag gatgagggg 
      ttgtgcaca ttggggggc gagatgtgc agttcgttg 
      agtgaaccc ccgcctgat atgttcgat ttaacggcc 
      gttttgtcc tctcagaag gaaaaggac gagtcgaat 
      ccgggttcg gtcactgag accagtagt ccgaatacg 
      ttgaatgag gattcgtgt gaaataccg gtacagccg 
      gagattgtc acgaagttg gacgaggag aaagaggcg 
      aattcgact gaggttgtg taaataaaa ctgtnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnat gcttatcgt agtgtatgt 
      tagtgtagc tccatgtgt catttttcg tgtgatgaa 
      gttggtact ggataggat tttaatctt gtttaagta 
      agcttagag gtaaagttc ttttcaata attacgtta 
      tgtataatt ttattgtaa taaaaaatt aatcagaca 
      tggttagct aagctaatg gtgccaaga tggcgtgtg 
      accttggct atgacatgt cactaaacc cagtcatcg 
      aataataca gactaaccc tgtatataa ttttttgct 
      actattgcc atgccgttt acttcatgt tcacaaatt 
      gttgtatac aaggtggtg aataaaagt tgcaaaaat 
      at
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