Status
Please wait ...
Gene information
General information
TC032520 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15127190..15128454
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032520
    iBeetle screen iB_07016

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032520 RA
      MSDTHSENN NIKFEIPKG DILIVAGDF 
      TRTGAPSEV NRFNEWMKS LPHQHKIVI 
      CGNHELSFD QTCQYIKGY YRSDIKNVL 
      CDCIYLEDS AVTIYGIKF YGSPWQPEF 
      GGWAFNVPR GEKCLSKWN LIPDDTDVL 
      ITHGPPLGY GDKITSKGN IGCVELLST 
      IQKRVKPKY HVFGHIHEG YGLYTDGTT 
      LFINASTCN RFFVPENLP IVFDYSLPS 
      GQTKENYM
    CDS sequence
    • >TC032520 RA
      atgagtgat acgcacagc gaaaataac aacataaaa 
      tttgaaata ccaaagggc gatattcta atagtagct 
      ggcgacttt acaagaact ggggcacca tctgaagta 
      aatagattc aacgagtgg atgaaatct ttaccacac 
      caacacaaa attgtaata tgtggaaat cacgaactg 
      tcgtttgac caaacgtgc cagtacata aaagggtat 
      tacagaagc gatattaaa aacgttttg tgtgattgc 
      atctacttg gaagattct gcagtgact atatatggt 
      attaaattt tacggttcc ccttggcag ccggaattt 
      ggtggatgg gcttttaat gtcccccgt ggagaaaag 
      tgtctttcc aaatggaat ttaattcca gatgatact 
      gatgttctg attactcat ggaccacca ttgggttat 
      ggcgacaaa ataacatca aagggaaat attggttgt 
      gtggagtta ttatcaaca attcaaaaa cgagttaaa 
      cccaaatat catgttttt ggccacatt cacgaagga 
      tatggttta tacaccgat ggaacaaca ttattcata 
      aatgcttca acttgtaac cgcttcttt gtgccagaa 
      aatctccct attgtgttt gattattcc ttaccaagt 
      ggccaaaca aaagaaaat tacatgtga
    mRNA sequence
    • >TC032520 RA
      tgcattcaa gtgggaatg ccgattttt gatagaagc 
      gtggcagac atccgtcat attggagaa actgggcca 
      gcatgaact ccaaactaa cagtacatc cactcagcg 
      ataagccct cagcagctt agaaagaga tttcaaaaa 
      cacaaaaat actctcaac ttgctatca ataatccgt 
      ccaatccta ttgcaaatg acagtgtta gaattgttt 
      gtatgagtg atacgcaca gcgaaaata acaacataa 
      aatttgaaa taccaaagg gcgatattc taatagtag 
      ctggcgact ttacaagaa ctggggcac catctgaag 
      taaatagat tcaacgagt ggatgaaat ctttaccac 
      accaacaca aaattgtaa tatgtggaa atcacgaac 
      tgtcgtttg accaaacgt gccagtaca taaaagggt 
      attacagaa gcgatatta aaaacgttt tgtgtgatt 
      gcatctact tggaagatt ctgcagtga ctatatatg 
      gtattaaat tttacggtt ccccttggc agccggaat 
      ttggtggat gggctttta atgtccccc gtggagaaa 
      agtgtcttt ccaaatgga atttaattc cagatgata 
      ctgatgttc tgattactc atggaccac cattgggtt 
      atggcgaca aaataacat caaagggaa atattggtt 
      gtgtggagt tattatcaa caattcaaa aacgagtta 
      aacccaaat atcatgttt ttggccaca ttcacgaag 
      gatatggtt tatacaccg atggaacaa cattattca 
      taaatgctt caacttgta accgcttct ttgtgccag 
      aaaatctcc ctattgtgt ttgattatt ccttaccaa 
      gtggccaaa caaaagaaa attacatgt gaggaactg 
      tttggtttt taattcttt tattgggat agtattttt 
      aaatttggc gccaaatta caggcgtga ctaccatta 
      agtattttt ctgaaaata gacgaagta ttcttttag 
      ttttgcctt atttatggt caacaactc ctatattat 
      cgtattatg gctttaatt attgtttta caaaaaagg 
      gtgttcagc cttaataat tattcggct gaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY