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Gene information
General information
TC032513 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14572736..14581188
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032513
    iBeetle screen iB_07012

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032513 RA
      MRNVPPKLV RYEDDAEYK KQPVDPIYI FRGVSPSDS 
      IALPEIPEN RKCTDLVMG GIFLIAVLS LIGSSVYIT 
      STSDIKILV NGADKCGNI CGVSNSESD NNKNCPEKD 
      YTNYPFLDV KAEECVTAD QCKARNKTL YGALCMPEQ 
      RRTPKSKSK LFAGRDYNK SLENIVPYT IIVLILMPS 
      VASGLSILV YQLLRSHPK CTFYFFTIL STIFFLLAA 
      CYFWYLFAT RDHENVDKN RTLVLLTSA AGFTISEVY 
      RETTKAIFH SKLVLLAPI LTMVLVNLV GAIWLFMML 
      VTFSVKIPE GETLPGART GYLIFLIFM GYWIFTFIE 
      GCQCMMVAG TIASYYFSR DRDNLKGQY CYNVKLVIR 
      FHLGSIATG ALLITIAAM IRILIDSLT SYSGSDTWA 
      WEIFRCMLL CIIRCLEEI VNYLTQKAY IMVAIHGKS 
      FWTSGKRAV KLIWHNFLD TISVDWLNS AVEFSASAL 
      ILFVTMLLG YAILSAQHM GFIIANLLF GGALAASAL 
      YVYFSLLST AVGTIFLCY CEDLLINDG SRERPFFMS 
      KEFQTTVFQ ALEFVVEKR EERKREDA
    CDS sequence
    • >TC032513 RA
      atgcgtaac gtgccacca aaattagtg agatatgaa 
      gatgatgct gaatataaa aagcaacca gtagatccc 
      atttatata tttcggggt gtaagtcct tcggattca 
      attgcttta ccagaaata cccgaaaat agaaaatgc 
      acggatctt gttatggga ggtattttt ttaattgcc 
      gttttaagt ctaattggt tcttctgtt tatataact 
      tcgacttct gatataaaa atcctagta aatggtgca 
      gataagtgt ggaaatatt tgtggagta tctaactct 
      gaatcagat aataataag aattgccca gaaaaagac 
      tacactaat tatccattt ctagatgtg aaagcagaa 
      gaatgtgtt acagctgac cagtgcaaa gcgagaaat 
      aaaacatta tatggtgcc ctatgtatg cctgaacaa 
      aggagaaca ccaaagtca aagtcgaaa ctatttgcg 
      ggtcgtgac tacaataaa tctctcgaa aacattgtt 
      ccatacaca ataatagtg ctaatttta atgccatct 
      gtggcttca ggattgtcc atactggta tatcaactg 
      cttcgaagc cacccaaag tgtactttc tattttttt 
      actattctt tctacgatt ttctttttg cttgcggct 
      tgctatttt tggtattta tttgctacc cgcgaccac 
      gaaaatgtc gataaaaac aggacactt gtattactt 
      actagtgcc gctggtttc acgataagt gaggtctat 
      cgtgaaaca actaaagca atttttcat tccaagctg 
      gtcttactt gcacctatc ttgacaatg gtgttggta 
      aacttagtt ggtgctatc tggttattt atgatgttg 
      gtgactttt tccgttaag attccggaa ggtgaaact 
      ttacctggt gccaggaca ggatacttg attttcctg 
      atatttatg ggatactgg atatttacg tttattgaa 
      ggatgtcag tgtatgatg gtagctgga acgatagca 
      agttactat ttttcgaga gatcgagat aatttgaag 
      ggtcaatat tgttataat gtgaaacta gtaattcgc 
      tttcatctg ggtagtatt gcaactgga gcactactt 
      attaccata gcagcaatg ataagaatt ttgattgac 
      tctcttact agttacagt gggtcagat acttgggcg 
      tgggaaatt tttagatgt atgctcctt tgcataatt 
      agatgtctc gaagagata gtgaattac ctcactcaa 
      aaagcgtac atcatggtt gccattcat ggtaaaagc 
      ttttggaca tctggcaag cgggcagtg aaattaata 
      tggcacaac tttttggac acgatatcc gttgattgg 
      ttaaactct gctgttgag ttttcagct tctgctcta 
      attttgttt gtcacgatg ctccttgga tatgccatt 
      ctatcagcg caacatatg ggttttata atcgcaaat 
      cttcttttt ggcggtgca cttgcagct agcgcatta 
      tacgtatac ttttcgttg ttgagcact gcggttgga 
      acaattttc ttgtgttat tgtgaagat ttattaata 
      aatgatgga agtagagag cggcctttt ttcatgagc 
      aaagagttt caaaccact gtgtttcaa gccttggaa 
      tttgttgtc gaaaaaaga gaagaaagg aagcgtgaa 
      gacgcctag
    mRNA sequence
    • >TC032513 RA
      ttccaaatt tgaatttac aaaaaaatt tttgtgcgc 
      gtaatttat ttcgtaaat attttgtaa aataccgtt 
      tacaatgcg taacgtgcc accaaaatt agtgagata 
      tgaagatga tgctgaata taaaaagca accagtaga 
      tcccattta tatatttcg gggtgtaag tccttcgga 
      ttcaattgc tttaccaga aatacccga aaatagaaa 
      atgcacgga tcttgttat gggaggtat ttttttaat 
      tgccgtttt aagtctaat tggttcttc tgtttatat 
      aacttcgac ttctgatat aaaaatcct agtaaatgg 
      tgcagataa gtgtggaaa tatttgtgg agtatctaa 
      ctctgaatc agataataa taagaattg cccagaaaa 
      agactacac taattatcc atttctaga tgtgaaagc 
      agaagaatg tgttacagc tgaccagtg caaagcgag 
      aaataaaac attatatgg tgccctatg tatgcctga 
      acaaaggag aacaccaaa gtcaaagtc gaaactatt 
      tgcgggtcg tgactacaa taaatctct cgaaaacat 
      tgttccata cacaataat agtgctaat tttaatgcc 
      atctgtggc ttcaggatt gtccatact ggtatatca 
      actgcttcg aagccaccc aaagtgtac tttctattt 
      ttttactat tctttctac gattttctt tttgcttgc 
      ggcttgcta tttttggta tttatttgc tacccgcga 
      ccacgaaaa tgtcgataa aaacaggac acttgtatt 
      acttactag tgccgctgg tttcacgat aagtgaggt 
      ctatcgtga aacaactaa agcaatttt tcattccaa 
      gctggtctt acttgcacc tatcttgac aatggtgtt 
      ggtaaactt agttggtgc tatctggtt atttatgat 
      gttggtgac tttttccgt taagattcc ggaaggtga 
      aactttacc tggtgccag gacaggata cttgatttt 
      cctgatatt tatgggata ctggatatt tacgtttat 
      tgaaggatg tcagtgtat gatggtagc tggaacgat 
      agcaagtta ctatttttc gagagatcg agataattt 
      gaagggtca atattgtta taatgtgaa actagtaat 
      tcgctttca tctgggtag tattgcaac tggagcact 
      acttattac catagcagc aatgataag aattttgat 
      tgactctct tactagtta cagtgggtc agatacttg 
      ggcgtggga aatttttag atgtatgct cctttgcat 
      aattagatg tctcgaaga gatagtgaa ttacctcac 
      tcaaaaagc gtacatcat ggttgccat tcatggtaa 
      aagcttttg gacatctgg caagcgggc agtgaaatt 
      aatatggca caacttttt ggacacgat atccgttga 
      ttggttaaa ctctgctgt tgagttttc agcttctgc 
      tctaatttt gtttgtcac gatgctcct tggatatgc 
      cattctatc agcgcaaca tatgggttt tataatcgc 
      aaatcttct ttttggcgg tgcacttgc agctagcgc 
      attatacgt atacttttc gttgttgag cactgcggt 
      tggaacaat tttcttgtg ttattgtga agatttatt 
      aataaatga tggaagtag agagcggcc ttttttcat 
      gagcaaaga gtttcaaac cactgtgtt tcaagcctt 
      ggaatttgt tgtcgaaaa aagagaaga aaggaagcg 
      tgaagacgc ctagaatca aaccaaaat tgttacaag 
      ttaaattcg attcgttat ctgttctga caaaaagaa 
      tcaattatt aattattat aaatcgata ttattatct 
      ttgtatatc ctttaaaaa aacaaatca ataaaatat 
      ttattaaat a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY