Status
Please wait ...
Gene information
General information
TC010525 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14393019..14433558
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010525
    iBeetle screen iB_07010

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010525 RA
      QLLVKQDLV GVCTSAECE SSDVSSVDC AERIRDTWW 
      TSAMRLHTI LSFGCLLVA TSAETPCNP KACSKDVDN 
      EKNVCGSDG LTYPNRCHF EKARCVNKN LTLAKRGPC 
      RQQKLCRDW EIYRHSNPD YKFHATCRP DGSYAAAQC 
      HPDTGFCWC VTPQGIPLP YTSVRWRPD AKPHCGRKK 
      KSTRRRSPP RKGRNRTCK QLDKAKFNN NLINIFHTE 
      WSRDHGGVI KPHNNESDL LVNQWKFGT MDADRNGVL 
      DKNEYRDLK RVAKKAVKP KKCAKMFPK SCDVNSDTL 
      ITQQEWAEC LTRDGMDDG GGINNSEDN GDDQEDYAD 
      AFPPHRPLP HALTYDDDS SEIRDEDPS DCLSDRQTA 
      LNDGGQFYV PECTPDGRY KKIQCYKAA GYCFCVHED 
      TGKNIPGTS VKNGKPKCD QLSNGNRPM KGCPNEKKT 
      IFLRDLIQF LHARMLKDN DTVNLYSLP WMSSKEEQA 
      ATWSFVEFD KNKNKMLDR NEWKIFKEV VAKEKNLRK 
      CGKKLPRYC DINKDRHIS MTEWLDCLN VQQGATTSA 
      PSSSRAGKK NPLDVLIED
    CDS sequence
    • >TC010525 RA
      caactcttg gtcaagcaa gatttagtt ggtgtctgt 
      acttcggcc gagtgtgaa tcgagcgat gtgtcgagt 
      gtagactgt gccgaacga atccgggac acttggtgg 
      acgtctgcg atgaggctg cacaccatc ctgtccttc 
      gggtgtcta ctcgtcgcc acttcagcg gaaactccc 
      tgcaacccg aaggcctgc agcaaagac gtggataac 
      gagaagaac gtttgcggt tccgacgga ctgacatat 
      ccgaatcgg tgtcatttt gagaaagct cgctgcgtc 
      aacaagaat ttaacattg gcgaaaagg ggcccttgc 
      cggcaacag aaactctgc cgcgattgg gagatctac 
      aggcattcg aaccccgac tacaaattt cacgcgaca 
      tgtcgccca gacggcagc tacgccgct gcccagtgc 
      caccctgac accggcttc tgctggtgc gtcactcct 
      cagggaatc cctctgccg tacacctcc gtgcgctgg 
      cgtcccgac gccaagccg cactgcggc cgcaagaag 
      aagtcgacc cggaggcgg tccccgcct cgcaagggc 
      cgcaaccgc acctgcaag cagctggac aaagccaaa 
      ttcaacaac aacctcatc aacatcttc cacacggag 
      tggagtcgc gaccacgga ggcgtcatc aagccgcac 
      aacaacgag agcgacctt ctggtcaac cagtggaag 
      ttcggcacc atggacgct gaccgcaac ggcgtcctc 
      gacaagaac gagtaccgg gacctgaag cgcgtcgcc 
      aagaaggcc gtcaagccc aagaagtgc gccaagatg 
      ttccccaag tcctgcgac gtcaacagc gacaccctc 
      atcacgcag caggagtgg gccgagtgc cttacgagg 
      gacggcatg gacgacggg ggtggaatc aacaattct 
      gaggataat ggggacgat caagaggac tacgccgac 
      gccttccct ccgcacagg cctctgccg catgcattg 
      acttacgac gacgactcc tcggaaatt cgcgacgaa 
      gaccccagt gattgtctt tcggaccgg caaacagcc 
      ttgaacgac ggcggccaa ttttatgtc ccggaatgc 
      acccccgat ggccgatat aaaaagatt cagtgctac 
      aaggcggcc ggatattgc ttctgcgtg cacgaagac 
      accggaaag aacattccg ggcacgtcg gtgaaaaat 
      ggaaagccc aaatgcgac caactttcg aacggaaac 
      cggcccatg aaaggctgt cccaacgag aaaaagact 
      atcttcctc cgagatctc atccagttc ctacatgct 
      cgcatgttg aaagataac gacacggtc aacctatac 
      agcctgccc tggatgtcc tcgaaagag gagcaggcg 
      gccacttgg agcttcgtc gaatttgac aagaacaag 
      aacaaaatg ctggatcgg aacgagtgg aagattttc 
      aaagaggtc gtcgcgaag gagaagaat ttgaggaag 
      tgcggcaag aagttgccg aggtactgt gatattaat 
      aaggaccgg cacataagc atgacggag tggctcgac 
      tgcctcaac gtgcagcaa ggagccaca acgagcgcc 
      cccagctcg tccagagcc ggcaaaaag aaccccctg 
      gatgtacta atagaagat tag
    mRNA sequence
    • >TC010525 RA
      agcaactct tggtcaagc aagatttag ttggtgtct 
      gtacttcgg ccgagtgtg aatcgagcg atgtgtcga 
      gtgtagact gtgccgaac gaatccggg acacttggt 
      ggacgtctg cgatgaggc tgcacacca tcctgtcct 
      tcgggtgtc tactcgtcg ccacttcag cggaaactc 
      cctgcaacc cgaaggcct gcagcaaag acgtggata 
      acgagaaga acgtttgcg gttccgacg gactgacat 
      atccgaatc ggtgtcatt ttgagaaag ctcgctgcg 
      tcaacaaga atttaacat tggcgaaaa ggggccctt 
      gccggcaac agaaactct gccgcgatt gggagatct 
      acaggcatt cgaaccccg actacaaat ttcacgcga 
      catgtcgcc cagacggca gctacgccg ctgcccagt 
      gccaccctg acaccggct tctgctggt gcgtcactc 
      ctcagggaa tccctctgc cgtacacct ccgtgcgct 
      ggcgtcccg acgccaagc cgcactgcg gccgcaaga 
      agaagtcga cccggaggc ggtccccgc ctcgcaagg 
      gccgcaacc gcacctgca agcagctgg acaaagcca 
      aattcaaca acaacctca tcaacatct tccacacgg 
      agtggagtc gcgaccacg gaggcgtca tcaagccgc 
      acaacaacg agagcgacc ttctggtca accagtgga 
      agttcggca ccatggacg ctgaccgca acggcgtcc 
      tcgacaaga acgagtacc gggacctga agcgcgtcg 
      ccaagaagg ccgtcaagc ccaagaagt gcgccaaga 
      tgttcccca agtcctgcg acgtcaaca gcgacaccc 
      tcatcacgc agcaggagt gggccgagt gccttacga 
      gggacggca tggacgacg ggggtggaa tcaacaatt 
      ctgaggata atggggacg atcaagagg actacgccg 
      acgccttcc ctccgcaca ggcctctgc cgcatgcat 
      tgacttacg acgacgact cctcggaaa ttcgcgacg 
      aagacccca gtgattgtc tttcggacc ggcaaacag 
      ccttgaacg acggcggcc aattttatg tcccggaat 
      gcacccccg atggccgat ataaaaaga ttcagtgct 
      acaaggcgg ccggatatt gcttctgcg tgcacgaag 
      acaccggaa agaacattc cgggcacgt cggtgaaaa 
      atggaaagc ccaaatgcg accaacttt cgaacggaa 
      accggccca tgaaaggct gtcccaacg agaaaaaga 
      ctatcttcc tccgagatc tcatccagt tcctacatg 
      ctcgcatgt tgaaagata acgacacgg tcaacctat 
      acagcctgc cctggatgt cctcgaaag aggagcagg 
      cggccactt ggagcttcg tcgaatttg acaagaaca 
      agaacaaaa tgctggatc ggaacgagt ggaagattt 
      tcaaagagg tcgtcgcga aggagaaga atttgagga 
      agtgcggca agaagttgc cgaggtact gtgatatta 
      ataaggacc ggcacataa gcatgacgg agtggctcg 
      actgcctca acgtgcagc aaggagcca caacgagcg 
      cccccagct cgtccagag ccggcaaaa agaaccccc 
      tggatgtac taatagaag attagtgat tttgtgaat 
      tgttgaaag actgggaac cccagcaaa ctgactggc 
      gcaagcctc gacttttgt gattatagt gtagtgtat 
      agctttcat cttatcgta ataatgcat cgtgcttat 
      ttatttttc aaaaataca accaaacct tgtatttta 
      tcccaactt tggttgctt gactcaaaa ttaaatcct 
      ctcggatgc ttgttgatg tatttttag agtaattaa 
      aaccatctt gttattgtc tgactattt aataaaaat 
      aaagtgaat aaaaatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY