Status
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Gene information
General information
TC003697 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12829137..12842874
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003697
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07002

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003697 RA
      METKSEESA QNRVLLEET FYVLTKKNC VFRVRLTKA 
      GLCLVKESE NNIKEQVIP IQDIIGCRC LRSKKQSKN 
      CACQSIPRS ANLKVVEEN SGDLDDTDV SAYLYIYAY 
      ILVNNKGTS KKRERTIIT LRFRSFDKY EDNNKEAQR 
      WRTMIKRLI KGENVSTSH IADFSVSNK YKEDRRLLV 
      LCNPKSGPG KGRIIFQQK VVPILQEAE IPYDLHITK 
      YANFAREFI RTCNIFQWS GIILVGGDG IVFEAINGL 
      FERWDWSDV VKTIPIGVI PGGSGNGLA RSIAYHCSE 
      PYLPSPTLP SALAAVRNN CAPMDLVRV ETTSQIMFS 
      FLSVGWGFL SDIDIESER LRMLGGQRF TVWSVARLI 
      GLRSYGGKL WYLPANVPL VPTRLEVNT GSALDLPAE 
      VHLETQTGR QRLDSWYSA ASRRSAYFS ATGSSYQST 
      ADSGGGGDP FGGDPDKPR MYGPASKLP CLTQPLDKE 
      TWKCIEGRF VMVHASYQT HLGEDCLFA PDAKLNDGT 
      IWLLIVHGG TTRTQLLHF LLGLSTGAH TSMVGEGGP 
      IELIAVNAF RIEPDMNEQ GYMTVDGEH VEYGPIQAE 
      VFPSLARVM VP
    CDS sequence
    • >TC003697 RA
      atggaaacg aaaagcgaa gaatcggcc caaaacagg 
      gtccttctg gaggagacc ttctacgtt ttgacgaag 
      aagaattgc gtgtttcgg gtccgcttg acgaaagcc 
      ggcctttgt ctagtgaaa gaaagcgag aacaacatc 
      aaagagcaa gtgattcca atccaagac atcataggc 
      tgcaggtgt ctcaggagc aagaaacag tcgaaaaat 
      tgcgcctgt cagtcaatt ccgcgctct gccaacctc 
      aaagtagtc gaggaaaat tcgggggat ttggacgac 
      acggacgtg agcgcctac ctctacatt tacgcttat 
      attttagtc aataacaag ggcacgagc aaaaagagg 
      gagcgcaca attatcacg ctgagattt cggtcgttt 
      gacaaatac gaagacaac aacaaggaa gcgcagagg 
      tggaggacg atgatcaag aggctgatc aagggcgag 
      aacgttagc acgagtcac attgccgat ttttccgtt 
      tcaaataag tacaaggaa gacaggagg ttgttggtg 
      ttgtgcaac cccaagagc gggcctggg aagggcagg 
      atcattttt caacaaaaa gtcgttcct attctacag 
      gaggcggaa attccttat gatttacat atcaccaaa 
      tatgctaat tttgcaaga gaatttatc aggacatgc 
      aatattttc cagtggagt ggaattatt ctggtcggc 
      ggtgacggc atcgtcttc gaagcaata aacggtctc 
      ttcgagcgc tgggactgg tccgacgtc gtcaaaacc 
      attccaatc ggtgtgatt cccggcggc tccggcaac 
      ggcctagcc cgcagcatc gcctaccac tgctcggag 
      ccctacctc ccttcacca accctaccc tccgccctc 
      gccgccgtt cgcaacaac tgcgccccc atggacctc 
      gtccgcgtc gaaacaacc tcccaaata atgttctcg 
      ttcttatca gtggggtgg ggcttcctt tccgacatc 
      gacatcgaa agcgagcgc ctccgcatg ctagggggg 
      caacggttc accgtgtgg tcggtcgcg agactcata 
      gggctgcgc agctacggg ggcaaatta tggtattta 
      ccggcgaac gtgcctttg gtgcccacc agactcgaa 
      gtgaacacc ggaagtgca ttagatctg cctgcggag 
      gtgcatttg gagactcag acggggcgt cagcggttg 
      gattcgtgg tacagtgcg gcgtcgcga cgcagcgcc 
      tatttttcg gcgacgggg agcagttat cagtcgacg 
      gcggatagt ggcggtggg ggcgacccc ttcgggggc 
      gatccggac aagccacgg atgtatggc cccgccagc 
      aagctccct tgtctcacg cagcccctt gataaagag 
      acatggaag tgcatagag gggcggttt gttatggtg 
      cacgcctcc tatcagacg catttgggg gaggattgc 
      cttttcgcc cccgatgcc aagctcaac gacggcacg 
      atttggttg ctgatcgtg catgggggc accacgagg 
      acgcagctg ttgcatttt ctgttgggg ctgagcacg 
      ggggcacat acgtcgatg gtgggcgaa gggggcccg 
      attgagctg atagcagtg aatgcgttc cggatagag 
      ccggatatg aacgaacag gggtatatg acggttgat 
      ggggaacat gttgagtat gggccgatt caagctgag 
      gtttttccg agtttggcg agagttatg gttccctga
    mRNA sequence
    • >TC003697 RA
      gttaaaatg atttgcgcc agtgagtga aaagttgta 
      aattgtgtt gcacgtagt tggaccttg acgctgtaa 
      aattcaaag ttaaaatga aaacgtaga gtggtttga 
      aatcagttc ggccgtgtt tcaagtcgt gtcaagtcc 
      gtacgagca tggaaacga aaagcgaag aatcggccc 
      aaaacaggg tccttctgg aggagacct tctacgttt 
      tgacgaaga agaattgcg tgtttcggg tccgcttga 
      cgaaagccg gcctttgtc tagtgaaag aaagcgaga 
      acaacatca aagagcaag tgattccaa tccaagaca 
      tcataggct gcaggtgtc tcaggagca agaaacagt 
      cgaaaaatt gcgcctgtc agtcaattc cgcgctctg 
      ccaacctca aagtagtcg aggaaaatt cgggggatt 
      tggacgaca cggacgtga gcgcctacc tctacattt 
      acgcttata ttttagtca ataacaagg gcacgagca 
      aaaagaggg agcgcacaa ttatcacgc tgagatttc 
      ggtcgtttg acaaatacg aagacaaca acaaggaag 
      cgcagaggt ggaggacga tgatcaaga ggctgatca 
      agggcgaga acgttagca cgagtcaca ttgccgatt 
      tttccgttt caaataagt acaaggaag acaggaggt 
      tgttggtgt tgtgcaacc ccaagagcg ggcctggga 
      agggcagga tcatttttc aacaaaaag tcgttccta 
      ttctacagg aggcggaaa ttccttatg atttacata 
      tcaccaaat atgctaatt ttgcaagag aatttatca 
      ggacatgca atattttcc agtggagtg gaattattc 
      tggtcggcg gtgacggca tcgtcttcg aagcaataa 
      acggtctct tcgagcgct gggactggt ccgacgtcg 
      tcaaaacca ttccaatcg gtgtgattc ccggcggct 
      ccggcaacg gcctagccc gcagcatcg cctaccact 
      gctcggagc cctacctcc cttcaccaa ccctaccct 
      ccgccctcg ccgccgttc gcaacaact gcgccccca 
      tggacctcg tccgcgtcg aaacaacct cccaaataa 
      tgttctcgt tcttatcag tggggtggg gcttccttt 
      ccgacatcg acatcgaaa gcgagcgcc tccgcatgc 
      taggggggc aacggttca ccgtgtggt cggtcgcga 
      gactcatag ggctgcgca gctacgggg gcaaattat 
      ggtatttac cggcgaacg tgcctttgg tgcccacca 
      gactcgaag tgaacaccg gaagtgcat tagatctgc 
      ctgcggagg tgcatttgg agactcaga cggggcgtc 
      agcggttgg attcgtggt acagtgcgg cgtcgcgac 
      gcagcgcct atttttcgg cgacgggga gcagttatc 
      agtcgacgg cggatagtg gcggtgggg gcgacccct 
      tcgggggcg atccggaca agccacgga tgtatggcc 
      ccgccagca agctccctt gtctcacgc agccccttg 
      ataaagaga catggaagt gcatagagg ggcggtttg 
      ttatggtgc acgcctcct atcagacgc atttggggg 
      aggattgcc ttttcgccc ccgatgcca agctcaacg 
      acggcacga tttggttgc tgatcgtgc atgggggca 
      ccacgagga cgcagctgt tgcattttc tgttggggc 
      tgagcacgg gggcacata cgtcgatgg tgggcgaag 
      ggggcccga ttgagctga tagcagtga atgcgttcc 
      ggatagagc cggatatga acgaacagg ggtatatga 
      cggttgatg gggaacatg ttgagtatg ggccgattc 
      aagctgagg tttttccga gtttggcga gagttatgg 
      ttccctgat cggaacatt ccttaggta ctaattgta 
      aaaataaat ctagggttg tgattttat ctactttag 
      agccactcc ttataaggc tcgatggca tttaatggt 
      ggtcgtttt gccggcttt aatcttgct gatttgttt 
      tttattatt atggagaga gaatcacga gctccaggt 
      attacataa cagctgatc aaactggcc tagctttaa 
      tacatagta ttacggcag cttgacttt aaaagccaa 
      gctatatta attgttatt aatttttgt tcacgaggc 
      agaaatctt attattcca gtgatacga acaccagtg 
      taaggaaaa aactttcat agttgagac aagcgtctc 
      aggagattt atctattga tctagttag gtaacatta 
      atgtaatga atgttgtta cttcattat aaggaatca 
      ctcataaat aggccatta ttactgaac ggttttaaa 
      atccaagat gccaatacc gtagtgcta aaaaatggt 
      acatttgtc aaaagtaga aatgagttt taatgtttc 
      tgagcacac aatgcatat agtaaaata gaccttcca 
      gaacaaggc tttaatcaa catagcttt acagtaaaa 
      attatttgt aaacgacta atttcatag taacaataa 
      aacagcaat atattttat gataatttt attcaaggt 
      atatagtct tatctttaa aaataccaa gtcaagtac 
      gtggtttac atgaaatgt aattggaag taggtacta 
      ttttatttg atattaaga aaaaaatcc accaaagat 
      ttaccgaaa ataatatta aaaaaatca acaattaca 
      tatttttta gttccgttc acattttta ccattattg 
      gaaaacgct gcgaatacg gttaaaaat tgaaagaac 
      attttaatt gtaatcaaa ttttaattc gagattaat 
      ttaacattt tttgtcaat gcattttaa atggcaact 
      taatgttga attagttta attctgaat taaatttta 
      atttcgctt tctataaat cgacccccc ccccctgaa 
      ttaaatttt ctttgaaaa aatccacta ttttcaatg 
      gtttacgtt caagacata acaaatgaa acgtttttg 
      ttactaggc atatttgat gccttcaga ccatcttta 
      gatcctgaa ccgtattgg taaacgggg gatcaaact 
      aaaatgttt atttcttta tttttttat tatttctta 
      ctgaagagc gaatacgaa tatgtgtgt aagtcgtgg 
      agggagaaa cttgtggag agaaggtct gaattttcc 
      gtcaagtgt acaatcagt gcgcaggtt tgaagctgt 
      gtcttcaat accataggc gtatcatag attctatac 
      cgtcaaatt atttggatt ctttttgac ttacagttt 
      tgtaatgta tagtaattt taaataatt gttagtact 
      aacggttag tgtttttaa agaagtgta ttttgacaa 
      tggttagct gcaaacttt aagtgcccc caaaattaa 
      aatatgttg actacacca ctggtttga aatttcaag 
      tgggaaaca aagtgcgcg tactttgaa aagggtaat 
      aaaaattgt ttgtgtaag gaccacggt ttctcgctt 
      atgttccct ccacaagtt tctccctct acggtgtaa 
      ggtctattt gtccataat tttgatcta gatattctg 
      aaaatgtag atgtaaaac tatttttta atttgtttc 
      tcataaatt taggatgga ttgacttgg tccttaaaa 
      aataattgg acataacat tgtatttgt ttcttgagc 
      gatataaaa aactcatgt acagtctgt ttcaagtct 
      tatcgccgg agcaacaat aaatctttc tagtcaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY