Status
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Gene information
General information
TC031394
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011392
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 735480..743806
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031394
    iBeetle screen iB_06999

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031394 RA
      MGCFKYTLI HLSTLLLLA HCATVKDIF NLNTRVPRD 
      NKYYPRYSS STTNLQPLN RAVKPEESP ESESPANPV 
      EKRCTRCDS SYSDSRYPY EARPRYDER RYRPYDYDD 
      RRYDRYDRY DDRRYDDRR YDRYDDRYE RDRYDRRPG 
      YDRYDRDRY DRYDRDRYD YGGRYDRYD RDYDRGFGY 
      DNRGYDYRD RGFGDYQTS GGGWDGGRG YGGRPEGGA 
      GYWGTGGYA SGWNYGGGR DEGRDRAYD GFKPWDETY 
      RGTSGWDSQ GRGFYFATG IPEVGTEHW GSKGYLDRD 
      RNPGPSDSH HTSVSPLIT IQPVDDKRY SGGGGGAGG 
      GGYYRPRDP YDGYRSTSY LYGRPSSTT TDQSPENDT 
      GVTQSPGQD NTSG
    • >TC031394 RB
      MGCFKYTLI HLSTLLLLA HCATVKDIF NLNTRVPRD 
      NKYYPRYSS STTNLQPLN RAVKPEESP ESESPANPV 
      EKRCTRCDS SYSDSRYPY EARPRYDER RYRPYDYDD 
      RRYDRYDRY DDRRYDDRR YDRYDDRYE RDRYDRRPG 
      YDRYDRDRY DRYDRDRYD YGGRYDRYD RDYDRGFGY 
      DNRGYDYRD RGFGDYQTS GGGWDGGRG YGGRPEGGA 
      GYWGTGGYA SGWNYGGGR DEGRDRAYD GFKPWDETY 
      RGTSGWDSQ GRGFYFATG IPEVGTEHW GSKGYLDRD 
      RYSGGGGGA GGGGYYRPR DPYDGYRST SYLYGRPSS 
      TTTDQSPEN DTGVTQSPG QDNTSG
    CDS sequence
    • >TC031394 RA
      atggggtgt tttaagtac accttgatc cacttatca 
      actctactt cttctggct cattgtgca accgtcaaa 
      gacatattt aacctcaac acacgtgtg ccacgtgac 
      aacaagtac tacccccgc tacagttcc agtactacc 
      aacctccaa ccgctaaac cgtgctgtc aaaccggag 
      gaatccccc gaaagcgaa tctcccgcc aatccggtt 
      gaaaaacga tgcacccga tgtgactcg agttattcc 
      gattcgcga tatccgtac gaggcgagg ccgaggtat 
      gacgaacgt cgataccgg ccatatgac tatgacgat 
      cgacgttat gaccgatat gaccgttat gacgatcgg 
      agatacgat gatcggaga tatgaccga tatgatgat 
      cggtatgaa agggaccgg tatgacagg aggccgggg 
      tatgaccgc tatgatagg gatcggtat gaccggtat 
      gatagggat cggtatgat tatgggggg aggtatgac 
      cggtatgat agggattat gaccgggga tttgggtac 
      gataatcgg gggtatgac tatagggat cggggattt 
      ggggattat cagacgtcg ggtggggga tgggatgga 
      gggaggggg tatggaggg cggccggaa gggggagcg 
      gggtactgg gggactggg gggtatgct agtggatgg 
      aactatgga ggagggagg gacgaagga agagatagg 
      gcttatgac ggcttcaaa ccgtgggat gagacatac 
      agaggaacc tctggatgg gatagccaa ggtcgagga 
      ttttatttc gcaacggga atccccgaa gttgggacc 
      gaacattgg ggaagtaaa ggatatcta gatcgggac 
      agaaatcct ggaccttcc gacagccat cacacatcg 
      gtttcccct ctaataacg atccagccg gtggacgat 
      aaacggtac agtggcgga ggcggaggg gcaggaggt 
      gggggttat taccgacct cgagatccc tatgacggc 
      taccggagt acaagctat ttgtatgga aggccgtcc 
      tcgaccacc accgaccag agcccggaa aatgacacc 
      ggagtgaca caaagtccg ggacaagac aacacctca 
      ggttaa
    • >TC031394 RB
      atggggtgt tttaagtac accttgatc cacttatca 
      actctactt cttctggct cattgtgca accgtcaaa 
      gacatattt aacctcaac acacgtgtg ccacgtgac 
      aacaagtac tacccccgc tacagttcc agtactacc 
      aacctccaa ccgctaaac cgtgctgtc aaaccggag 
      gaatccccc gaaagcgaa tctcccgcc aatccggtt 
      gaaaaacga tgcacccga tgtgactcg agttattcc 
      gattcgcga tatccgtac gaggcgagg ccgaggtat 
      gacgaacgt cgataccgg ccatatgac tatgacgat 
      cgacgttat gaccgatat gaccgttat gacgatcgg 
      agatacgat gatcggaga tatgaccga tatgatgat 
      cggtatgaa agggaccgg tatgacagg aggccgggg 
      tatgaccgc tatgatagg gatcggtat gaccggtat 
      gatagggat cggtatgat tatgggggg aggtatgac 
      cggtatgat agggattat gaccgggga tttgggtac 
      gataatcgg gggtatgac tatagggat cggggattt 
      ggggattat cagacgtcg ggtggggga tgggatgga 
      gggaggggg tatggaggg cggccggaa gggggagcg 
      gggtactgg gggactggg gggtatgct agtggatgg 
      aactatgga ggagggagg gacgaagga agagatagg 
      gcttatgac ggcttcaaa ccgtgggat gagacatac 
      agaggaacc tctggatgg gatagccaa ggtcgagga 
      ttttatttc gcaacggga atccccgaa gttgggacc 
      gaacattgg ggaagtaaa ggatatcta gatcgggac 
      aggtacagt ggcggaggc ggaggggca ggaggtggg 
      ggttattac cgacctcga gatccctat gacggctac 
      cggagtaca agctatttg tatggaagg ccgtcctcg 
      accaccacc gaccagagc ccggaaaat gacaccgga 
      gtgacacaa agtccggga caagacaac acctcaggt 
      taa
    mRNA sequence
    • >TC031394 RA
      cttgtttgg tgtcggtgc tgggttgct gcagtgggt 
      gaatgcttg gctgactcg gatgaccac catggggtg 
      ttttaagta caccttgat ccacttatc aactctact 
      tcttctggc tcattgtgc aaccgtcaa agacatatt 
      taacctcaa cacacgtgt gccacgtga caacaagta 
      ctacccccg ctacagttc cagtactac caacctcca 
      accgctaaa ccgtgctgt caaaccgga ggaatcccc 
      cgaaagcga atctcccgc caatccggt tgaaaaacg 
      atgcacccg atgtgactc gagttattc cgattcgcg 
      atatccgta cgaggcgag gccgaggta tgacgaacg 
      tcgataccg gccatatga ctatgacga tcgacgtta 
      tgaccgata tgaccgtta tgacgatcg gagatacga 
      tgatcggag atatgaccg atatgatga tcggtatga 
      aagggaccg gtatgacag gaggccggg gtatgaccg 
      ctatgatag ggatcggta tgaccggta tgataggga 
      tcggtatga ttatggggg gaggtatga ccggtatga 
      tagggatta tgaccgggg atttgggta cgataatcg 
      ggggtatga ctataggga tcggggatt tggggatta 
      tcagacgtc gggtggggg atgggatgg agggagggg 
      gtatggagg gcggccgga agggggagc ggggtactg 
      ggggactgg ggggtatgc tagtggatg gaactatgg 
      aggagggag ggacgaagg aagagatag ggcttatga 
      cggcttcaa accgtggga tgagacata cagaggaac 
      ctctggatg ggatagcca aggtcgagg attttattt 
      cgcaacggg aatccccga agttgggac cgaacattg 
      gggaagtaa aggatatct agatcggga cagaaatcc 
      tggaccttc cgacagcca tcacacatc ggtttcccc 
      tctaataac gatccagcc ggtggacga taaacggta 
      cagtggcgg aggcggagg ggcaggagg tgggggtta 
      ttaccgacc tcgagatcc ctatgacgg ctaccggag 
      tacaagcta tttgtatgg aaggccgtc ctcgaccac 
      caccgacca gagcccgga aaatgacac cggagtgac 
      acaaagtcc gggacaaga caacacctc aggttaatt 
      ttaaggaat cttattgtg caatttctt tttcaatca 
      agtagattt taatgtatt tacttacaa aactgcctt 
      gtttattta atcaaccaa agaacatta acagtattt 
      catttcttg acgtgtatt taatttaat tattttata 
      ataaaggtt tttgagata a
    • >TC031394 RB
      cttgtttgg tgtcggtgc tgggttgct gcagtgggt 
      gaatgcttg gctgactcg gatgaccac catggggtg 
      ttttaagta caccttgat ccacttatc aactctact 
      tcttctggc tcattgtgc aaccgtcaa agacatatt 
      taacctcaa cacacgtgt gccacgtga caacaagta 
      ctacccccg ctacagttc cagtactac caacctcca 
      accgctaaa ccgtgctgt caaaccgga ggaatcccc 
      cgaaagcga atctcccgc caatccggt tgaaaaacg 
      atgcacccg atgtgactc gagttattc cgattcgcg 
      atatccgta cgaggcgag gccgaggta tgacgaacg 
      tcgataccg gccatatga ctatgacga tcgacgtta 
      tgaccgata tgaccgtta tgacgatcg gagatacga 
      tgatcggag atatgaccg atatgatga tcggtatga 
      aagggaccg gtatgacag gaggccggg gtatgaccg 
      ctatgatag ggatcggta tgaccggta tgataggga 
      tcggtatga ttatggggg gaggtatga ccggtatga 
      tagggatta tgaccgggg atttgggta cgataatcg 
      ggggtatga ctataggga tcggggatt tggggatta 
      tcagacgtc gggtggggg atgggatgg agggagggg 
      gtatggagg gcggccgga agggggagc ggggtactg 
      ggggactgg ggggtatgc tagtggatg gaactatgg 
      aggagggag ggacgaagg aagagatag ggcttatga 
      cggcttcaa accgtggga tgagacata cagaggaac 
      ctctggatg ggatagcca aggtcgagg attttattt 
      cgcaacggg aatccccga agttgggac cgaacattg 
      gggaagtaa aggatatct agatcggga caggtacag 
      tggcggagg cggaggggc aggaggtgg gggttatta 
      ccgacctcg agatcccta tgacggcta ccggagtac 
      aagctattt gtatggaag gccgtcctc gaccaccac 
      cgaccagag cccggaaaa tgacaccgg agtgacaca 
      aagtccggg acaagacaa cacctcagg ttaatttta 
      aggaatctt attgtgcaa tttcttttt caatcaagt 
      agattttaa tgtatttac ttacaaaac tgccttgtt 
      tatttaatc aaccaaaga acattaaca gtatttcat 
      ttcttgacg tgtatttaa tttaattat tttataata 
      aaggttttt gagataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY