Status
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Gene information
General information
TC002775 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11682083..11700083
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002775
    iBeetle screen iB_06996

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    many wildtypic hatched larva, often sheding

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002775 RA
      MPSDTRVAA GETALLQCG APRGHPEPN LMWRKDGEV 
      LEVDGRRIR IVDGGNLMI SDVRQHDEG RYQCVAHNL 
      VGSRETPPV MLTVHVKPF FNKEPQDVV ALLGKRVVF 
      QCAVDGEPV PNVLWRRED GKMPIGRAR ILDDKSLLI 
      DNVQTSDEG IYICAAENV VGSITAKAS LVVNSPPTF 
      IEKPRDQKV SLNGVVEFH CSATGNPPP SVFWTKEGS 
      QMLMFPDNS YGHMHVNAH GTLRIQGVQ REDAGFLVC 
      SALSVAGSS SVRAFLQVT SVADLPPPI IQIGPANQT 
      LPLHSMVTL HCKASSPEG EQPKIRWSK DGKVLQEER 
      LPDRYTISE TGTLDIDDL RLDDSGLYT CSAISESGE 
      SSWSASLSV VEGSSALLH RSPDPSTFP KAPNQPRIL 
      NATESSITL AWDDGPEAG LVGYTVEYW SPDLQTGWV 
      IAAHRVSNY IVVRELKPD SSYMFIVRA ENAHGLSPA 
      SPVSSIART LANSRAVSG SELDTARTA LSTKLVELR 
      DARPQSSSS VKLTWSLAA LDYIEGLYI RFRELSGGS 
      HNYNILTVL DVQTKSYTV SNLKKFTKY EFFIAPFYK 
      SVEGQPSNS RVAQTLEDV PSAPPDGVT AGVFNDTAG 
      WVRWSPPPP QHHNGIIIG YKIQIRGSI TRSLTVNSS 
      TTSVLISNL TSGGSYNAR IAALTHAGQ GPYSNQVPL 
      LTAPRAQSP RTLGPFVPL VQQTWFVVL LATAILAIL 
      VITGGLLYL KKRQSLSKQ LGHLNVPVV NASQLNAKD 
      TLWIDRGWR QTDCDKDSS IPLAQPADY AEVDTRSLS 
      TFYNCKKSP ENPTPYATT MLLPPPNWS EFIPPPPDH 
      PPPECPPVP PPRGGSSCG SGYACYAVH VPKQYNRCG 
      NCSLASGSS GRRSTPRGH CHPHNCDKV WSNQQWEDG 
      DADVETDRH SCSSSHDTT CSCSESSCL YAEAGPSNP 
      RPVPHCQVK RCGK
    CDS sequence
    • >TC002775 RA
      atgccgtcc gatacgaga gtggcggcg ggcgaaacc 
      gcgcttctc cagtgcggg gcgccaaga gggcatccc 
      gagccgaat ctcatgtgg aggaaggac ggggaagtg 
      ctggaggtg gacggcagg aggatcagg attgtcgat 
      ggaggcaat ctcatgatc tcggatgtt agacaacac 
      gacgaaggg cgatatcag tgcgtggcg cataatttg 
      gtggggtcg agggagacg ccgcccgtt atgctcaca 
      gtacacgtg aagccattc ttcaataag gaaccccaa 
      gacgtggtg gccctcctg ggcaagagg gtggtgttc 
      cagtgcgcg gtggacggg gagcccgtc cccaacgtg 
      ctgtggcgg cgcgaggac ggcaagatg cccatcggc 
      cgcgcccgc atcctcgac gacaagtcg ctgctcatc 
      gacaacgtg cagacctcc gacgagggc atttacatc 
      tgcgcggcc gagaacgtc gtcggcagc atcacggcc 
      aaggcatcg ctcgtcgtt aattctccg ccaacgttc 
      atcgaaaag ccccgcgac cagaaggtc tccctcaac 
      ggcgtcgtc gagttccac tgctcggcc accggaaac 
      ccccctccg tccgtcttc tggaccaaa gaaggttcc 
      caaatgctc atgtttccg gacaattcc tacggtcat 
      atgcacgtg aacgctcac ggaacgctc cgaatccaa 
      ggagtgcaa cgtgaggat gccggattt ttagtctgt 
      tcggcttta agtgttgca ggctcgagc agtgtgcga 
      gcattcctg caggtcacg tcggtggca gatctcccg 
      ccaccaatt atacaaatt ggacctgct aatcaaacg 
      ctaccactg cacagcatg gtcactttg cactgcaaa 
      gcctccagt cccgaaggg gaacagccc aagattcgc 
      tggtccaag gacgggaag gtgctgcag gaggagcgc 
      ttgcccgat agatacacc atcagcgag acgggaacg 
      ctagatatt gacgatttg cgtttggac gattcgggc 
      ctctacacc tgctccgcc atctcggaa agcggcgag 
      agctcgtgg tcggcctcc ctcagcgta gtcgagggc 
      tcctcagcc ctcctgcac cgcagtccc gacccgtcc 
      acctttcca aaagcccca aaccagccc cgcatcctc 
      aacgccact gaatcaagc atcacccta gcatgggac 
      gacggtccc gaagccggc cttgtgggc tacaccgtc 
      gagtactgg agccccgac ctgcagacc ggctgggtc 
      atcgcggcc catcgcgtc tccaactac atcgtagtg 
      cgtgaactt aaacccgac agttcgtac atgtttata 
      gtgcgcgcc gaaaacgcc catggactc tcgcccgca 
      agtccggtc tcgtccatt gcccgcact ctggccaac 
      tcgcgggcg gtctccggg tccgagctg gacacagcc 
      cggacggcc ctcagcacg aagttggtc gagctaagg 
      gacgccaga ccacagtcg agcagttcg gttaaattg 
      acgtggagt ttagcagcg ttggattat atcgaaggg 
      ttgtatata cggtttcgg gagttgtcc gggggttcc 
      cacaattat aacatactg actgtgctt gatgtgcaa 
      acgaagagc tatacagtg tcgaatttg aagaagttt 
      acgaaatat gagtttttt atcgcgccg ttctataaa 
      tcggttgag ggtcagccg agtaattct agagttgcg 
      caaacgctg gaagacgtt ccttcggca ccgcctgat 
      ggtgtcact gctggggtt tttaacgac acagccggg 
      tgggtccgt tggtcgcct cccccaccc caacaccac 
      aatggtatt ataattggg tacaaaatc cagatccga 
      gggtccatt acccggtct ttgacagtc aattcgagc 
      actacttcc gttttgatc agtaaccta acttccggt 
      gggtcgtac aatgcccga attgcagct ttgacgcat 
      gctggccaa ggcccttat tccaaccaa gtgccctta 
      ttgaccgcc ccgcgggcc caaagcccg cgcaccttg 
      ggccccttt gtccccttg gtccaacaa acctggttc 
      gtggtccta ctcgcgact gcaattctc gcaattctg 
      gtaatcacc ggaggactc ctctacttg aaaaaacgc 
      cagtctttg agcaaacaa ctgggccat ttaaacgtc 
      ccggtggtc aacgccagt caactgaac gcaaaagac 
      acactctgg atcgacagg ggctggcgc cagaccgac 
      tgcgacaaa gactcctcc atccccttg gcccaaccg 
      gccgactac gccgaagtc gacacacgc agcctctcc 
      accttctac aactgcaaa aaatccccc gagaacccg 
      accccgtac gccaccacc atgctcctg ccgccgccg 
      aactggtcc gagttcatc ccgccgcca ccggaccac 
      ccgccgccc gagtgcccg ccggtgccg ccgccgcgg 
      ggcggctcc agctgcggc agcgggtac gcgtgctac 
      gccgtgcac gtgcccaag cagtacaac cggtgcggg 
      aactgctcg ttggcgagc ggcagctcc gggaggcgg 
      tccaccccc cgggggcac tgccacccc cataactgt 
      gataaagtg tggagcaat cagcagtgg gaggatggc 
      gatgccgat gtcgagact gatagacat tcctgttcg 
      agtagtcac gacaccacc tgttcctgt agtgagtcg 
      tcgtgtttg tacgccgag gcggggccg agtaatccg 
      cggccggtg cctcattgc caggtgaaa aggtgcggg 
      aagtga
    mRNA sequence
    • >TC002775 RA
      tgcagtgct gagagacga gtttcgggt catgccgtc 
      cgatacgag agtggcggc gggcgaaac cgcgcttct 
      ccagtgcgg ggcgccaag agggcatcc cgagccgaa 
      tctcatgtg gaggaagga cggggaagt gctggaggt 
      ggacggcag gaggatcag gattgtcga tggaggcaa 
      tctcatgat ctcggatgt tagacaaca cgacgaagg 
      gcgatatca gtgcgtggc gcataattt ggtggggtc 
      gagggagac gccgcccgt tatgctcac agtacacgt 
      gaagccatt cttcaataa ggaacccca agacgtggt 
      ggccctcct gggcaagag ggtggtgtt ccagtgcgc 
      ggtggacgg ggagcccgt ccccaacgt gctgtggcg 
      gcgcgagga cggcaagat gcccatcgg ccgcgcccg 
      catcctcga cgacaagtc gctgctcat cgacaacgt 
      gcagacctc cgacgaggg catttacat ctgcgcggc 
      cgagaacgt cgtcggcag catcacggc caaggcatc 
      gctcgtcgt taattctcc gccaacgtt catcgaaaa 
      gccccgcga ccagaaggt ctccctcaa cggcgtcgt 
      cgagttcca ctgctcggc caccggaaa cccccctcc 
      gtccgtctt ctggaccaa agaaggttc ccaaatgct 
      catgtttcc ggacaattc ctacggtca tatgcacgt 
      gaacgctca cggaacgct ccgaatcca aggagtgca 
      acgtgagga tgccggatt tttagtctg ttcggcttt 
      aagtgttgc aggctcgag cagtgtgcg agcattcct 
      gcaggtcac gtcggtggc agatctccc gccaccaat 
      tatacaaat tggacctgc taatcaaac gctaccact 
      gcacagcat ggtcacttt gcactgcaa agcctccag 
      tcccgaagg ggaacagcc caagattcg ctggtccaa 
      ggacgggaa ggtgctgca ggaggagcg cttgcccga 
      tagatacac catcagcga gacgggaac gctagatat 
      tgacgattt gcgtttgga cgattcggg cctctacac 
      ctgctccgc catctcgga aagcggcga gagctcgtg 
      gtcggcctc cctcagcgt agtcgaggg ctcctcagc 
      cctcctgca ccgcagtcc cgacccgtc cacctttcc 
      aaaagcccc aaaccagcc ccgcatcct caacgccac 
      tgaatcaag catcaccct agcatggga cgacggtcc 
      cgaagccgg ccttgtggg ctacaccgt cgagtactg 
      gagccccga cctgcagac cggctgggt catcgcggc 
      ccatcgcgt ctccaacta catcgtagt gcgtgaact 
      taaacccga cagttcgta catgtttat agtgcgcgc 
      cgaaaacgc ccatggact ctcgcccgc aagtccggt 
      ctcgtccat tgcccgcac tctggccaa ctcgcgggc 
      ggtctccgg gtccgagct ggacacagc ccggacggc 
      cctcagcac gaagttggt cgagctaag ggacgccag 
      accacagtc gagcagttc ggttaaatt gacgtggag 
      tttagcagc gttggatta tatcgaagg gttgtatat 
      acggtttcg ggagttgtc cgggggttc ccacaatta 
      taacatact gactgtgct tgatgtgca aacgaagag 
      ctatacagt gtcgaattt gaagaagtt tacgaaata 
      tgagttttt tatcgcgcc gttctataa atcggttga 
      gggtcagcc gagtaattc tagagttgc gcaaacgct 
      ggaagacgt tccttcggc accgcctga tggtgtcac 
      tgctggggt ttttaacga cacagccgg gtgggtccg 
      ttggtcgcc tcccccacc ccaacacca caatggtat 
      tataattgg gtacaaaat ccagatccg agggtccat 
      tacccggtc tttgacagt caattcgag cactacttc 
      cgttttgat cagtaacct aacttccgg tgggtcgta 
      caatgcccg aattgcagc tttgacgca tgctggcca 
      aggccctta ttccaacca agtgccctt attgaccgc 
      cccgcgggc ccaaagccc gcgcacctt gggcccctt 
      tgtcccctt ggtccaaca aacctggtt cgtggtcct 
      actcgcgac tgcaattct cgcaattct ggtaatcac 
      cggaggact cctctactt gaaaaaacg ccagtcttt 
      gagcaaaca actgggcca tttaaacgt cccggtggt 
      caacgccag tcaactgaa cgcaaaaga cacactctg 
      gatcgacag gggctggcg ccagaccga ctgcgacaa 
      agactcctc catcccctt ggcccaacc ggccgacta 
      cgccgaagt cgacacacg cagcctctc caccttcta 
      caactgcaa aaaatcccc cgagaaccc gaccccgta 
      cgccaccac catgctcct gccgccgcc gaactggtc 
      cgagttcat cccgccgcc accggacca cccgccgcc 
      cgagtgccc gccggtgcc gccgccgcg gggcggctc 
      cagctgcgg cagcgggta cgcgtgcta cgccgtgca 
      cgtgcccaa gcagtacaa ccggtgcgg gaactgctc 
      gttggcgag cggcagctc cgggaggcg gtccacccc 
      ccgggggca ctgccaccc ccataactg tgataaagt 
      gtggagcaa tcagcagtg ggaggatgg cgatgccga 
      tgtcgagac tgatagaca ttcctgttc gagtagtca 
      cgacaccac ctgttcctg tagtgagtc gtcgtgttt 
      gtacgccga ggcggggcc gagtaatcc gcggccggt 
      gcctcattg ccaggtgaa aaggtgcgg gaagtgaac 
      gctaccgca cgcgggcgg aaagtgacg ttgacatat 
      cggtgcgga aagtgactt gtacagcat atcaggggg 
      gccgaaggc ccccaatgt cctaatacg actactagt 
      ccaagaaaa ttagactcg tttggtaat atttacaaa 
      aattcggaa gggagataa agcagaatt gctattatc 
      tcgcgcagc tcgttatat ttttacagc gctgcctga 
      ataagttaa taaaaccgc cggaacgtt aaaactcca 
      tctcaattt aatttcgtc aagttttat cttatcgtt 
      gacctcgcg tcgaaattg aaacattaa aaattttgc 
      taattatgc tttgttttg ataaaatcg ttgctaaaa 
      caaaatatt catgctcat ttttatgta atttgttta 
      cagaatcag ctgaaagca atttatttc gacgataag 
      ccacgaaaa atgacacaa aaatctaac attaaaaca 
      tttccattc agagatata tcacgtttg cttgtttca 
      ttaaaaaca atttttttc ggtgcttat ctattgtct 
      tttaattaa ttatgtttt tgccttcat tgttcttat 
      tatgtagca tttgtgtca atttgccgc agatgatca 
      cggtcagaa atttaatta cctattgaa gtagaggct 
      cagttaata gctatttat acgccaaga ggagaaaga 
      catgtattc tttcccgaa atgaagatg aataaataa 
      tcatctgtt ttacatatg atttttttc gacggcttc 
      atcagaaat atcgttttc attttccaa atagaatat 
      gaaagttcg agggaggaa aatgagggg ggaaaatgt 
      tttcatccc tgcagatgt atcttcatc gatattttc 
      taatgtggc gaatatttt attttttcg ttgaccgtt 
      tgttattaa tgaacgact tgtgtttat ggccgtcat 
      taatgcact catttttat ttattcatt aataatgca 
      cccttttcg ggattatgg taatgagcg attaataat 
      cgtgtttag agcacgaac gaacgacat gagtgcctg 
      ccttaaata gttaattaa gtaactgtt tgttctgga 
      tccagctgt actcggcag tttgaacca aatgctacg 
      taacatttt aagcttgtg acttggttt tgtgttgaa 
      aaaatgacg atttcgtct aattttgct ggactattc 
      gttgcctag tcctagttt cgcgtttta gttaccagt 
      aagtcgaat gtgtcttcc tgttttaac tagcacaaa 
      gcagacttg tcgtctcgc agttggaat tcgttttcg 
      tgtcatatt agcgactca tcttaggcc ataagtacc 
      atgagtgta agattttgg ttgaactgc ttttgtaga 
      tattgccaa agaactgcc tagaccccc ccgggtccc 
      cataatcca atacaatct cagactgaa ttcctatac 
      ttctttagc aatacttat gaacttgta agtaggatt 
      ttatattta tattatcga ttgtgattt agtcttaaa 
      gagtattta aacgtgtat atatgaatt gtgagcttg 
      tatataaat acattgtta tacctgtgg agaccactc 
      ggtatttgt aactttttg tagaaaaaa attcataaa 
      tgtaagata aatattgat gtagatatt tattatttt 
      cctgttatt tataattga ataaacggt acttgcata 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY