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Gene information
General information
TC000133 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10746624..10749124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC000133
      iBeetle screen iB_06991

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC000133 RA
        MSVEEEVEL RDMVAQTLE LNGCLPKIR AQLRANLFL 
        ALDEDSKIS KQQPLLNTK IKSYLEVPE GQLMFCLVR 
        EFLEYFDLD FTASVYEPE SYVGSFYKY EGRDKIIED 
        LGLKPSDSF SGPVLQHLL KVAQIKSKT LKINLNVND 
        ELKETNGHD SEAENSILS NKNSHNLSE HSTHINNNK 
        NDNLDSTYI KRDAENDDT FNGTSSIEE ENGSCDKST 
        NEDSTTDQD TSPKSEDKS KKSDKLKTK LELPPLNKS 
        RTDLLPSLY SKKSSKEYD FEIKDDYEE DFMSGSEIE 
        LSGGKPLFE ELQQAKCDN KSSNSTDIE TNLSVETNS 
        SL
      CDS sequence
      • >TC000133 RA
        atgtcagtt gaggaagaa gtggaattg cgagacatg 
        gtggctcaa accctcgaa ttaaacggt tgcctcccg 
        aaaatacga gcacagttg agagcgaac ctatttctt 
        gccttagac gaagattct aaaataagt aagcaacag 
        ccattgtta aacacaaaa atcaagtca tacttagaa 
        gtgccagaa ggtcagctc atgttttgt ctcgttaga 
        gagttcctt gaatatttt gacctcgat ttcactgcg 
        tctgtttac gagcctgaa tcatacgtt ggcagcttt 
        tacaagtac gaaggacgc gacaaaatc atcgaagat 
        ttgggctta aaaccgtca gacagtttt tccggtcct 
        gtcctccaa cacttgctt aaagttgct caaatcaag 
        tcaaaaacg ctgaaaatc aatttgaat gttaacgac 
        gaactgaag gaaactaac ggacacgac tctgaagct 
        gaaaactcc atccttagt aacaaaaac agccataat 
        ttaagcgag cactccaca catatcaat aacaataaa 
        aacgataat ttagattcc acgtacatt aagcgagac 
        gctgaaaat gatgatacg ttcaatgga acaagttcc 
        atagaagaa gaaaacggc agctgtgac aaatcaacg 
        aatgaagat agtacaacg gatcaggac accagtcca 
        aaatctgaa gataagtca aagaaaagt gacaagctt 
        aaaacaaaa ctcgagttg ccaccttta aacaaatca 
        agaactgat ctactgcct tcactgtat agcaaaaag 
        tcgagcaaa gagtacgat ttcgaaatt aaagacgac 
        tacgaggag gatttcatg tccgggagc gaaatcgag 
        ttatccggg ggcaagccg ttgtttgag gagttgcaa 
        caagcaaag tgtgacaat aaatcaagt aactcaacg 
        gatattgag acaaattta agtgtggag acgaattct 
        agtctctaa
      mRNA sequence
      • >TC000133 RA
        ggcggttgg tgtaagggg ctataacac cgaaaacat 
        ttaactgat cgaattttt gatgagttc aactaataa 
        tttgtcgga taaaaagcc gctatttgg cataagcgt 
        gtcaagttt caaaatgca caccgagac atcttcaaa 
        tttttccgg gcggctgca gccccacca agatgagct 
        ccaattatt aggcgaatt ggcaggtgt tcacatgga 
        tgtaaaagt tttcaggat tttggtcga attcttgaa 
        cttgggggt agccccgaa tacgatttt ttcggtctt 
        gaagagcat aaggcggtc gccgtcaag acggctatc 
        acattcggc acatcactg acttgtaaa tttaataat 
        aaaaaaatt gagctgagg tgtttgttt ggtgtccgc 
        tggtttaaa aatgacgtt tgacaagtg acaggtgat 
        tgaaatggg ggttgaggg aacgcaatt ttttttcat 
        ttaggtctt gttttctgt tgttttaat gaaattgct 
        cagttgttt gtgctgtga ttgacaatt cgttgcaaa 
        ccgtgtgtt taagtggga aaatgtcag ttgaggaag 
        aagtggaat tgcgagaca tggtggctc aaaccctcg 
        aattaaacg gttgcctcc cgaaaatac gagcacagt 
        tgagagcga acctatttc ttgccttag acgaagatt 
        ctaaaataa gtaagcaac agccattgt taaacacaa 
        aaatcaagt catacttag aagtgccag aaggtcagc 
        tcatgtttt gtctcgtta gagagttcc ttgaatatt 
        ttgacctcg atttcactg cgtctgttt acgagcctg 
        aatcatacg ttggcagct tttacaagt acgaaggac 
        gcgacaaaa tcatcgaag atttgggct taaaaccgt 
        cagacagtt tttccggtc ctgtcctcc aacacttgc 
        ttaaagttg ctcaaatca agtcaaaaa cgctgaaaa 
        tcaatttga atgttaacg acgaactga aggaaacta 
        acggacacg actctgaag ctgaaaact ccatcctta 
        gtaacaaaa acagccata atttaagcg agcactcca 
        cacatatca ataacaata aaaacgata atttagatt 
        ccacgtaca ttaagcgag acgctgaaa atgatgata 
        cgttcaatg gaacaagtt ccatagaag aagaaaacg 
        gcagctgtg acaaatcaa cgaatgaag atagtacaa 
        cggatcagg acaccagtc caaaatctg aagataagt 
        caaagaaaa gtgacaagc ttaaaacaa aactcgagt 
        tgccacctt taaacaaat caagaactg atctactgc 
        cttcactgt atagcaaaa agtcgagca aagagtacg 
        atttcgaaa ttaaagacg actacgagg aggatttca 
        tgtccggga gcgaaatcg agttatccg ggggcaagc 
        cgttgtttg aggagttgc aacaagcaa agtgtgaca 
        ataaatcaa gtaactcaa cggatattg agacaaatt 
        taagtgtgg agacgaatt ctagtctct aagttttgg 
        ccacagcct cctaacatt tagtttgaa tcaatagga 
        ggcggtggt tgcgatcaa cgctcactt gtacttaat 
        ggcttctca ctagagtta gagttttgt acttaattt 
        tgaatgtgc cttaaattt gtgattatt tttaataaa 
        gtaggtgac tacaaaaca actgtattt acagatcat 
        tacaaacaa cactatctc attctacac aagtggcgt 
        tttcttttc acatgttgt ttcgctctc tttggattc 
        gcaaactgt acgctttcg agaccaaat tccacgagt 
        ccatgtacc tgtccatac acatggcta cgcatttct 
        ggaaaacat tggtaaggt taggtttga aaaggcgga 
        attcacctg ttcggaact gtccaggga cgtgcccgg 
        cttgtttat acatttctt aaagcattt ttcggtcat 
        tttctgtga aaaaatgga tggaatttg aatcggtaa 
        aaatgtgcc agcttacag tgagaagtt cttgcgcgt 
        ttgcgacgg cgatttgtt gcttgactt gatccatta 
        gttcgtctt tttgggctc cggttaatc cacccccta 
        gtgagtcca ttattaatg gtttaatta tttatttta 
        ggtgaaata atttaactg cgattttta ggttatgac 
        aggcaaaga agggggatg ccaacctag atacgttaa 
        aattaccaa acttggtag tattcggac aaaacagac 
        ttggcagcg aatccaatt cgggtgatt tgtgcttgt 
        aaacattga aatgatttt aataaaagt atttgtaca 
        at
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY