Status
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Gene information
General information
TC000132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10755307..10766623
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000132
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06990

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000132 RA
      MKKLFSKIE TKIDNTSKE GNSYIGKVF TVGRQTVTV 
      EDILAEGGF AIVYLVKGT NGTRYALKR MYVNNEQDL 
      NVAKREIQI ASSLSGHKN IIGYVDSSL TATGGGVYE 
      VLLLMPYCQ ENVFGLMKQ RGKANFTEH EVLTIFCDT 
      CEAVSRLHH CKTPIIHRD LKVENILVG ENGNYVICD 
      FGSATAKVL NPADKGVAP VEEEIKRYT TLSYRAPEM 
      VDMYCGKPI TTKADIWAL GCLLYRLCF FTLPFGEST 
      LAIQSGNFS IPDNSKYSK GLHQLMRYM LEPDPDKRP 
      DIFQVSCVA FQLLSKENP VKNLMKSPI PKLEDLPVP 
      PFESELKKA QVKVYNKTP AAPIVEGTS VMPRQRPKG 
      SSTTPLNLN NIPLTLSPS PTTSKKSQS PVTQPPVTT 
      YPSNFQTQP TFEPVKQLD SLFQSSIYP DPFRDDNNA 
      TESPTNEAN NGGAQVKSP ASPENPLFG SGNLNQGAP 
      AKPSLTESS SYVGATMTP PSTPTLAVP KGHRRNMSD 
      TTAFNKVYA SETSQFLAP YESSMKPRS SDSPEMIKG 
      PLVPPGVSA STADINHPI APDSRSLSA DVADWNPFE 
      EPPFSQMTE DHIFGEEFD KIRRGSQSS IQGVKSRES 
      LVMSVSEDP FGCAPFSLP VRSRDRINK TQLQFSVLT 
      QKIKEDDQE LIAYSPPQQ SETEAPLIQ TELVTSPPY 
      VKAPLEDRS KYEKLTQSN YISSESSSD ENNADKVKK 
      KRKTIPEKL QSVYKTVEL PIKFRNEKK TQKKVKHKS 
      DSVDIESDD SIGSASDLR IDEDTEHVK GDAISETVS 
      ESIKTCGSS AYHAECESM ATHEDDSTN RMIRAKIKE 
      EAKKSTVQD EDMLFVGHK YGDKPLLLD DELDSDCEV 
      KFKQWPEKR AATDLWIPP SSSFEDEDV FALAPFNKP 
      RSKSKTEKN SNPFLDFPN PVQSTSSYG TVTVNSNFI 
      NIEIPANFN VTRFESNFT EVDPYFPPP PETKEIIYE 
      NVNIPEKTE PGYDLFDFA PTEVEPVSN VTFSLEASH 
      HKHKKDKKK SKYHLIDES VSDESPSIN FPKSAKIVK 
      PGYRKVAKN KSHKCKTKS QGGFSNMSF EDFSSDDRE 
      EVERSVMMP FEVLRSPEQ EDRKFATKR ISNPFS
    • >TC000132 RB
      MKKLFSKIE TKIDNTSKE GNSYIGKVF TVGRQTVTV 
      EDILAEGGF AIVYLVKGT NGTRYALKR MYVNNEQDL 
      NVAKREIQI ASSLSGHKN IIGYVDSSL TATGGGVYE 
      VLLLMPYCQ ENVFGLMKQ RGKANFTEH EVLTIFCDT 
      CEAVSRLHH CKTPIIHRD LKVENILVG ENGNYVICD 
      FGSATAKVL NPADKGVAP VEEEIKRYT TLSYRAPEM 
      VDMYCGKPI TTKADIWAL GCLLYRLCF FTLPFGEST 
      LAIQSGNFS IPDNSKYSK GLHQLMRYM LEPDPDKRP 
      DIFQVSCVA FQLLSKENP VKNLMKSPI PKLEDLPVP 
      PFESELKKA QVKVYNKTP AAPIVEGTS VMPRQRPKG 
      SSTTPLNLN NIPLTLSPS PTTSKKSQS PVTQPPVTT 
      YPSNFQTQP TFEPVKQLD SLFQSSIYP DPFRDDNNA 
      TESPTNEAN NGGAQVKSP ASPENPLFG SGNLNQGAP 
      AKPSLTESS SYVGATMTP PSTPTLAVP KGHRRNMSD 
      TTAFNKVYA SETSQFLAP YESSMKPRS SDSPEMIKG 
      PLVPPGVSA STADINHPI APDSRSLSA DVADWNPFE 
      EPPFSQMTE DHIFGEEFD KIRRGSQSS IQGVKSRES 
      LVMSVSEDP FGCAPFSLP VRSRDRINK TQLQFSETP 
      VQYVNVAVE WAK
    CDS sequence
    • >TC000132 RA
      atgaaaaaa ctgttttct aaaatcgaa actaaaata 
      gataacact tcgaaggaa ggtaacagt tacatagga 
      aaagtgttc acagtcggc cgccaaacg gtaaccgtc 
      gaggatatt ctagccgaa ggtggtttt gcgatcgtg 
      tatctggta aagggcacc aatggcacg cgctacgca 
      ctcaaacga atgtacgtt aataacgag caggacctt 
      aacgttgcc aaacgtgaa attcaaatc gctagcagt 
      ttaagtggc cataaaaac atcattggt tatgtagat 
      tccagtttg acggccaca ggagggggc gtttatgaa 
      gtattgttg ttgatgcct tattgtcag gaaaacgtt 
      ttcggtctt atgaaacaa cgtggcaaa gcgaatttt 
      acggaacat gaagttctg accattttc tgtgatact 
      tgcgaggcc gtgtcacga ttgcatcac tgcaaaact 
      ccaataatt catcgtgat ttaaaggta gaaaatatt 
      ttggtaggt gaaaatggt aactacgtg atatgtgat 
      tttggttcg gccactgcg aaagtatta aacccggct 
      gataagggg gttgctccc gttgaagaa gagattaaa 
      cggtacaca acactgagt tatagggca cctgagatg 
      gtcgacatg tattgtggc aaaccaatc acgacgaaa 
      gctgatatt tgggctctt ggctgttta ttgtataga 
      ttatgcttt tttacgtta ccgtttggt gaaagtact 
      ttagctata caaagtggg aactttagc attccagat 
      aattcgaaa tattcgaaa ggtttacat cagctaatg 
      cgctacatg ttggagccc gatcctgac aaaaggcct 
      gacattttc caagtcagc tgcgtcgcc tttcaactc 
      ctttcgaaa gaaaacccc gtcaaaaac ctaatgaaa 
      tcgcccatt cccaagctg gaggacctt ccagtgcct 
      ccgttcgag tccgaactg aagaaagct caagtcaaa 
      gtgtacaac aaaacgccg gcagccccc atcgtggaa 
      ggcacgagt gtgatgccc cgccagcgt cccaaagga 
      agctccaca acacctctc aaccttaac aacatccca 
      ctaacccta agtcccagt cctaccacc agtaaaaaa 
      tcacaaagt cccgtaact caaccacct gttaccaca 
      tacccttct aattttcag acccaacca acattcgaa 
      cccgtcaag caattggac tccctcttt cagtcttcc 
      atctatcct gatcccttt cgagacgat aataatgca 
      actgaatcg ccaacaaac gaggctaat aatgggggc 
      gcccaagtc aaatcgcct gcctcaccc gaaaacccc 
      ctatttggt tcggggaat ttgaatcag ggggcaccc 
      gcaaaaccg agtttgact gaatcaagt agttacgtg 
      ggggcgacg atgacgccg ccctctaca ccgaccttg 
      gccgtaccc aaaggacac cggaggaac atgagcgac 
      acgacggcg tttaataag gtttatgcc tcggaaacg 
      tcgcagttt ctggcccct tatgagtcg tccatgaag 
      ccgcgcagc agcgattcg cctgaaatg ataaagggg 
      cctctggtg ccccctggc gtttcggca tcaactgct 
      gatataaac cacccgata gcccccgat agtcgatca 
      ctttcggcc gacgtggct gactggaat cccttcgag 
      gagccccct tttagtcaa atgacggaa gaccacatt 
      ttcggtgaa gaattcgat aagattagg agaggaagt 
      cagagtagt attcaaggt gttaagtca agagaaagt 
      ctagtaatg tcggtgtct gaagatccg ttcggttgt 
      gcaccgttc agtcttcca gtcaggtca cgtgatcgt 
      attaacaag actcagctc cagttctca gttttgacg 
      caaaaaatc aaagaagat gatcaagaa ttgatcgct 
      tattcgccc cctcaacag tccgaaacg gaagcacct 
      ttgattcaa accgaatta gtcacatca ccaccgtac 
      gtcaaagcg ccacttgaa gaccgttcc aagtacgaa 
      aaactaaca cagagtaat tacatcagt tccgaaagt 
      agttcagac gaaaataac gccgataaa gtgaaaaag 
      aagcgtaaa acaataccg gaaaaacta caatcggtt 
      tataaaact gtcgaattg ccgatcaag ttccgcaac 
      gagaaaaaa acacaaaag aaggtgaaa cacaagagt 
      gactctgtc gatattgaa tcggacgat tcgattggt 
      tcggctagt gatttgcga atcgacgaa gacacagaa 
      catgttaaa ggggacgcg attagcgaa acagttagc 
      gaaagtatt aaaacgtgc ggctcgtcg gcttatcac 
      gctgaatgc gaaagcatg gcaacgcac gaagacgac 
      agcactaat cgaatgata agagcgaaa attaaggag 
      gaagcaaaa aagagcaca gttcaagat gaagacatg 
      ctcttcgtt ggtcataaa tacggcgat aagccgttg 
      ctattggac gacgaattg gactcggac tgtgaggtc 
      aagttcaaa caatggcct gaaaaacgc gccgccact 
      gatttgtgg attccccca tccagcagt ttcgaagac 
      gaagatgtt ttcgctttg gcgccgttc aacaaacca 
      cgatccaaa tcaaaaact gagaaaaac tcaaacccg 
      ttcctcgat tttccgaat ccggtccaa agcacgtcg 
      agttacggc acagtgact gtaaattcg aatttcatt 
      aatatcgaa attcccgcc aacttcaac gtgacccga 
      ttcgagtcg aacttcacc gaagtcgac ccctatttc 
      ccgccaccg cccgaaacc aaagaaatt atctacgaa 
      aatgtcaat attcccgaa aagaccgaa ccggggtac 
      gatttgttc gatttcgca cccaccgaa gtcgagcct 
      gtctcgaac gttaccttt agtctagaa gccagccac 
      cacaaacac aagaaagac aagaagaag tccaagtac 
      catttgatc gatgagagc gttagtgac gagagcccc 
      agtattaat tttcccaag agtgcgaaa attgttaag 
      cccggttac cggaaagtg gcgaaaaac aagtcgcac 
      aagtgcaag acgaaatcg caaggtggg tttagtaat 
      atgagtttt gaggatttc tcgtccgat gatcgcgaa 
      gaggtcgaa cggtctgtg atgatgcct tttgaagtg 
      ttgcgtagt cccgaacag gaggatagg aaatttgca 
      acaaaacgg atcagtaat ccgttttct taa
    • >TC000132 RB
      atgaaaaaa ctgttttct aaaatcgaa actaaaata 
      gataacact tcgaaggaa ggtaacagt tacatagga 
      aaagtgttc acagtcggc cgccaaacg gtaaccgtc 
      gaggatatt ctagccgaa ggtggtttt gcgatcgtg 
      tatctggta aagggcacc aatggcacg cgctacgca 
      ctcaaacga atgtacgtt aataacgag caggacctt 
      aacgttgcc aaacgtgaa attcaaatc gctagcagt 
      ttaagtggc cataaaaac atcattggt tatgtagat 
      tccagtttg acggccaca ggagggggc gtttatgaa 
      gtattgttg ttgatgcct tattgtcag gaaaacgtt 
      ttcggtctt atgaaacaa cgtggcaaa gcgaatttt 
      acggaacat gaagttctg accattttc tgtgatact 
      tgcgaggcc gtgtcacga ttgcatcac tgcaaaact 
      ccaataatt catcgtgat ttaaaggta gaaaatatt 
      ttggtaggt gaaaatggt aactacgtg atatgtgat 
      tttggttcg gccactgcg aaagtatta aacccggct 
      gataagggg gttgctccc gttgaagaa gagattaaa 
      cggtacaca acactgagt tatagggca cctgagatg 
      gtcgacatg tattgtggc aaaccaatc acgacgaaa 
      gctgatatt tgggctctt ggctgttta ttgtataga 
      ttatgcttt tttacgtta ccgtttggt gaaagtact 
      ttagctata caaagtggg aactttagc attccagat 
      aattcgaaa tattcgaaa ggtttacat cagctaatg 
      cgctacatg ttggagccc gatcctgac aaaaggcct 
      gacattttc caagtcagc tgcgtcgcc tttcaactc 
      ctttcgaaa gaaaacccc gtcaaaaac ctaatgaaa 
      tcgcccatt cccaagctg gaggacctt ccagtgcct 
      ccgttcgag tccgaactg aagaaagct caagtcaaa 
      gtgtacaac aaaacgccg gcagccccc atcgtggaa 
      ggcacgagt gtgatgccc cgccagcgt cccaaagga 
      agctccaca acacctctc aaccttaac aacatccca 
      ctaacccta agtcccagt cctaccacc agtaaaaaa 
      tcacaaagt cccgtaact caaccacct gttaccaca 
      tacccttct aattttcag acccaacca acattcgaa 
      cccgtcaag caattggac tccctcttt cagtcttcc 
      atctatcct gatcccttt cgagacgat aataatgca 
      actgaatcg ccaacaaac gaggctaat aatgggggc 
      gcccaagtc aaatcgcct gcctcaccc gaaaacccc 
      ctatttggt tcggggaat ttgaatcag ggggcaccc 
      gcaaaaccg agtttgact gaatcaagt agttacgtg 
      ggggcgacg atgacgccg ccctctaca ccgaccttg 
      gccgtaccc aaaggacac cggaggaac atgagcgac 
      acgacggcg tttaataag gtttatgcc tcggaaacg 
      tcgcagttt ctggcccct tatgagtcg tccatgaag 
      ccgcgcagc agcgattcg cctgaaatg ataaagggg 
      cctctggtg ccccctggc gtttcggca tcaactgct 
      gatataaac cacccgata gcccccgat agtcgatca 
      ctttcggcc gacgtggct gactggaat cccttcgag 
      gagccccct tttagtcaa atgacggaa gaccacatt 
      ttcggtgaa gaattcgat aagattagg agaggaagt 
      cagagtagt attcaaggt gttaagtca agagaaagt 
      ctagtaatg tcggtgtct gaagatccg ttcggttgt 
      gcaccgttc agtcttcca gtcaggtca cgtgatcgt 
      attaacaag actcagctc cagttctca gagacgcct 
      gtacaatat gtaaacgtt gctgttgaa tgggccaag 
      tag
    mRNA sequence
    • >TC000132 RA
      actttctta aacgtcact gacactgtt atgcggaca 
      attatttta atcaattaa taaaaatag tcgatttga 
      agcggtagc aagtgtgag gtgggctgt cgtaacggt 
      ctggagcca gaaaattga tggggaatc cgcattcac 
      tcaccaaac tcaatgtgt cataatttc caattaagt 
      gtcaatcac gatgaaaaa actgttttc taaaatcga 
      aactaaaat agataacac ttcgaagga aggtaacag 
      ttacatagg aaaagtgtt cacagtcgg ccgccaaac 
      ggtaaccgt cgaggatat tctagccga aggtggttt 
      tgcgatcgt gtatctggt aaagggcac caatggcac 
      gcgctacgc actcaaacg aatgtacgt taataacga 
      gcaggacct taacgttgc caaacgtga aattcaaat 
      cgctagcag tttaagtgg ccataaaaa catcattgg 
      ttatgtaga ttccagttt gacggccac aggaggggg 
      cgtttatga agtattgtt gttgatgcc ttattgtca 
      ggaaaacgt tttcggtct tatgaaaca acgtggcaa 
      agcgaattt tacggaaca tgaagttct gaccatttt 
      ctgtgatac ttgcgaggc cgtgtcacg attgcatca 
      ctgcaaaac tccaataat tcatcgtga tttaaaggt 
      agaaaatat tttggtagg tgaaaatgg taactacgt 
      gatatgtga ttttggttc ggccactgc gaaagtatt 
      aaacccggc tgataaggg ggttgctcc cgttgaaga 
      agagattaa acggtacac aacactgag ttatagggc 
      acctgagat ggtcgacat gtattgtgg caaaccaat 
      cacgacgaa agctgatat ttgggctct tggctgttt 
      attgtatag attatgctt ttttacgtt accgtttgg 
      tgaaagtac tttagctat acaaagtgg gaactttag 
      cattccaga taattcgaa atattcgaa aggtttaca 
      tcagctaat gcgctacat gttggagcc cgatcctga 
      caaaaggcc tgacatttt ccaagtcag ctgcgtcgc 
      ctttcaact cctttcgaa agaaaaccc cgtcaaaaa 
      cctaatgaa atcgcccat tcccaagct ggaggacct 
      tccagtgcc tccgttcga gtccgaact gaagaaagc 
      tcaagtcaa agtgtacaa caaaacgcc ggcagcccc 
      catcgtgga aggcacgag tgtgatgcc ccgccagcg 
      tcccaaagg aagctccac aacacctct caaccttaa 
      caacatccc actaaccct aagtcccag tcctaccac 
      cagtaaaaa atcacaaag tcccgtaac tcaaccacc 
      tgttaccac atacccttc taattttca gacccaacc 
      aacattcga acccgtcaa gcaattgga ctccctctt 
      tcagtcttc catctatcc tgatccctt tcgagacga 
      taataatgc aactgaatc gccaacaaa cgaggctaa 
      taatggggg cgcccaagt caaatcgcc tgcctcacc 
      cgaaaaccc cctatttgg ttcggggaa tttgaatca 
      gggggcacc cgcaaaacc gagtttgac tgaatcaag 
      tagttacgt gggggcgac gatgacgcc gccctctac 
      accgacctt ggccgtacc caaaggaca ccggaggaa 
      catgagcga cacgacggc gtttaataa ggtttatgc 
      ctcggaaac gtcgcagtt tctggcccc ttatgagtc 
      gtccatgaa gccgcgcag cagcgattc gcctgaaat 
      gataaaggg gcctctggt gccccctgg cgtttcggc 
      atcaactgc tgatataaa ccacccgat agcccccga 
      tagtcgatc actttcggc cgacgtggc tgactggaa 
      tcccttcga ggagccccc ttttagtca aatgacgga 
      agaccacat tttcggtga agaattcga taagattag 
      gagaggaag tcagagtag tattcaagg tgttaagtc 
      aagagaaag tctagtaat gtcggtgtc tgaagatcc 
      gttcggttg tgcaccgtt cagtcttcc agtcaggtc 
      acgtgatcg tattaacaa gactcagct ccagttctc 
      agttttgac gcaaaaaat caaagaaga tgatcaaga 
      attgatcgc ttattcgcc ccctcaaca gtccgaaac 
      ggaagcacc tttgattca aaccgaatt agtcacatc 
      accaccgta cgtcaaagc gccacttga agaccgttc 
      caagtacga aaaactaac acagagtaa ttacatcag 
      ttccgaaag tagttcaga cgaaaataa cgccgataa 
      agtgaaaaa gaagcgtaa aacaatacc ggaaaaact 
      acaatcggt ttataaaac tgtcgaatt gccgatcaa 
      gttccgcaa cgagaaaaa aacacaaaa gaaggtgaa 
      acacaagag tgactctgt cgatattga atcggacga 
      ttcgattgg ttcggctag tgatttgcg aatcgacga 
      agacacaga acatgttaa aggggacgc gattagcga 
      aacagttag cgaaagtat taaaacgtg cggctcgtc 
      ggcttatca cgctgaatg cgaaagcat ggcaacgca 
      cgaagacga cagcactaa tcgaatgat aagagcgaa 
      aattaagga ggaagcaaa aaagagcac agttcaaga 
      tgaagacat gctcttcgt tggtcataa atacggcga 
      taagccgtt gctattgga cgacgaatt ggactcgga 
      ctgtgaggt caagttcaa acaatggcc tgaaaaacg 
      cgccgccac tgatttgtg gattccccc atccagcag 
      tttcgaaga cgaagatgt tttcgcttt ggcgccgtt 
      caacaaacc acgatccaa atcaaaaac tgagaaaaa 
      ctcaaaccc gttcctcga ttttccgaa tccggtcca 
      aagcacgtc gagttacgg cacagtgac tgtaaattc 
      gaatttcat taatatcga aattcccgc caacttcaa 
      cgtgacccg attcgagtc gaacttcac cgaagtcga 
      cccctattt cccgccacc gcccgaaac caaagaaat 
      tatctacga aaatgtcaa tattcccga aaagaccga 
      accggggta cgatttgtt cgatttcgc acccaccga 
      agtcgagcc tgtctcgaa cgttacctt tagtctaga 
      agccagcca ccacaaaca caagaaaga caagaagaa 
      gtccaagta ccatttgat cgatgagag cgttagtga 
      cgagagccc cagtattaa ttttcccaa gagtgcgaa 
      aattgttaa gcccggtta ccggaaagt ggcgaaaaa 
      caagtcgca caagtgcaa gacgaaatc gcaaggtgg 
      gtttagtaa tatgagttt tgaggattt ctcgtccga 
      tgatcgcga agaggtcga acggtctgt gatgatgcc 
      ttttgaagt gttgcgtag tcccgaaca ggaggatag 
      gaaatttgc aacaaaacg gatcagtaa tccgttttc 
      ttaagcagc tttgtgacg taaggcagc tgtggtttt 
      gttactatt tattcaaat tcaagctta tgcgtggta 
      aggccacga tttatttat tcaaactga tgatattgg 
      catacctgg ttgggggtc cccttatta acttgtgtg 
      acatttatc aaatatggc gttaggact ttttccacg 
      gcactgcga tttgggtaa aaattttga gcacgttta 
      cacgccgtt ttgtgatcg ttattaaaa gaccaaatc 
      taggctttg tgctttgat acttgatgt taggggtcg 
      tacaatgaa taaataaca catttacta tcacatttc 
      taaacagaa atttcttta tttccaaca ttttaataa 
      caacgctgt gtaaacgta gtcataaat cacgattgc 
      cgtgaatga cgacatttt gttaaatgt caaaaattt 
      aagtgatgg tgattgtgt ccaggtatg ttaatttat 
      tttttgtgt tagtagttg aagacaaga ctgtaatta 
      ttacgttac ttaagcatt atttaaagt ctgagtaaa 
      actgcgtca agtgtttcg gtgcctttt ccaatcaaa 
      atttgcaaa agaataacg aatgaaaac gctaccaat 
      ccgttgata ataataagc caaagtgta agcaaactt 
      gaatttcct acatatagc aagtcaatt aaaacgctt 
      ttattgtga catctaggt cctgtttct agtgtataa 
      gatatgaaa cctagttga tgtttaaaa gattattta 
      tttataaaa aaagaagtg tatacattg ttcgtataa 
      aaacattcc atttattta tatttgtag tactattta 
      aatattaat tgcactttt gtaacttgt aatatttta 
      taaaacgca tacaaataa atttgttgt cttaaagtc 
      attgttacg ttacaaata cggaggact aaagcgtag 
      gacacgaaa cgagataca aaagcgcac gatattctg 
      tacagaaaa aatatagta cgatagaat aaaaatgag 
      ggcgctgca agcgccaaa taatcgttt taaattaat 
      ttaaattca acaaattca ctaataact tcgaagaaa 
      atacagaaa tgttgagct ttacattct taatagacc 
      gagtaaatc taactgcta aaattttca caagaaaca 
      attgaaaat agtatatta aaaaacaag ttttataag 
      cacaaattc agtttcaaa aacgagtgg cgttattca 
      cgagttatt tgaaagaac atgtgcttc aaggtctaa 
      tgaaacgac ctctttata ctgtttata ccctttttt 
      ccgttttta aacagaaat ttttatttt aattgattt 
      agggatttt tctgacgat tagtaatga aagtgaaaa 
      acgtatttt aaatttttg ttttatccc aattgggtc 
      aaaaaaaca ggagtatgg atgataata aagatactc 
      ttgcaaa
    • >TC000132 RB
      actttctta aacgtcact gacactgtt atgcggaca 
      attatttta atcaattaa taaaaatag tcgatttga 
      agcggtagc aagtgtgag gtgggctgt cgtaacggt 
      ctggagcca gaaaattga tggggaatc cgcattcac 
      tcaccaaac tcaatgtgt cataatttc caattaagt 
      gtcaatcac gatgaaaaa actgttttc taaaatcga 
      aactaaaat agataacac ttcgaagga aggtaacag 
      ttacatagg aaaagtgtt cacagtcgg ccgccaaac 
      ggtaaccgt cgaggatat tctagccga aggtggttt 
      tgcgatcgt gtatctggt aaagggcac caatggcac 
      gcgctacgc actcaaacg aatgtacgt taataacga 
      gcaggacct taacgttgc caaacgtga aattcaaat 
      cgctagcag tttaagtgg ccataaaaa catcattgg 
      ttatgtaga ttccagttt gacggccac aggaggggg 
      cgtttatga agtattgtt gttgatgcc ttattgtca 
      ggaaaacgt tttcggtct tatgaaaca acgtggcaa 
      agcgaattt tacggaaca tgaagttct gaccatttt 
      ctgtgatac ttgcgaggc cgtgtcacg attgcatca 
      ctgcaaaac tccaataat tcatcgtga tttaaaggt 
      agaaaatat tttggtagg tgaaaatgg taactacgt 
      gatatgtga ttttggttc ggccactgc gaaagtatt 
      aaacccggc tgataaggg ggttgctcc cgttgaaga 
      agagattaa acggtacac aacactgag ttatagggc 
      acctgagat ggtcgacat gtattgtgg caaaccaat 
      cacgacgaa agctgatat ttgggctct tggctgttt 
      attgtatag attatgctt ttttacgtt accgtttgg 
      tgaaagtac tttagctat acaaagtgg gaactttag 
      cattccaga taattcgaa atattcgaa aggtttaca 
      tcagctaat gcgctacat gttggagcc cgatcctga 
      caaaaggcc tgacatttt ccaagtcag ctgcgtcgc 
      ctttcaact cctttcgaa agaaaaccc cgtcaaaaa 
      cctaatgaa atcgcccat tcccaagct ggaggacct 
      tccagtgcc tccgttcga gtccgaact gaagaaagc 
      tcaagtcaa agtgtacaa caaaacgcc ggcagcccc 
      catcgtgga aggcacgag tgtgatgcc ccgccagcg 
      tcccaaagg aagctccac aacacctct caaccttaa 
      caacatccc actaaccct aagtcccag tcctaccac 
      cagtaaaaa atcacaaag tcccgtaac tcaaccacc 
      tgttaccac atacccttc taattttca gacccaacc 
      aacattcga acccgtcaa gcaattgga ctccctctt 
      tcagtcttc catctatcc tgatccctt tcgagacga 
      taataatgc aactgaatc gccaacaaa cgaggctaa 
      taatggggg cgcccaagt caaatcgcc tgcctcacc 
      cgaaaaccc cctatttgg ttcggggaa tttgaatca 
      gggggcacc cgcaaaacc gagtttgac tgaatcaag 
      tagttacgt gggggcgac gatgacgcc gccctctac 
      accgacctt ggccgtacc caaaggaca ccggaggaa 
      catgagcga cacgacggc gtttaataa ggtttatgc 
      ctcggaaac gtcgcagtt tctggcccc ttatgagtc 
      gtccatgaa gccgcgcag cagcgattc gcctgaaat 
      gataaaggg gcctctggt gccccctgg cgtttcggc 
      atcaactgc tgatataaa ccacccgat agcccccga 
      tagtcgatc actttcggc cgacgtggc tgactggaa 
      tcccttcga ggagccccc ttttagtca aatgacgga 
      agaccacat tttcggtga agaattcga taagattag 
      gagaggaag tcagagtag tattcaagg tgttaagtc 
      aagagaaag tctagtaat gtcggtgtc tgaagatcc 
      gttcggttg tgcaccgtt cagtcttcc agtcaggtc 
      acgtgatcg tattaacaa gactcagct ccagttctc 
      agagacgcc tgtacaata tgtaaacgt tgctgttga 
      atgggccaa gtagtcagt gtttaaaaa aacaattaa 
      aacaaagtt tgaaatgaa aaaattagt gaaatttca 
      cagtagcac agccgcttt gatggctag aaaacgttc 
      atagatggc tgacagtaa gctgtaagg tacaatttt 
      attcgttgt aatgacgtt tttaaggtc attagttgg 
      ctgcgaccg gccgtgtag gaacgcagt tcctattca 
      tcaaagtgg ctgtgagaa gtaggctta gataataac 
      tagtgtact tgataataa taatgcgtg atggcaatg 
      ttgcttcga ttgttttca tcgcaggcc aatattttt 
      tccctatat ctggcctgc aatttctat aagatcccc 
      ttgtttaaa tgttcaaaa tatccaaaa caatgtgat 
      aatcactaa tgagaacat aatctttat aatacttgt 
      gttatgtgg accaatatt gagtttttt catttatta 
      ttagagaag taagtcgtc cgtgtgcaa tagtttgta 
      tgtcgtact taagataga taggagaga aaacgatgg 
      ctaaatttt ttatcattc caaatgaaa gttgaaaaa 
      aaaatacag acggattcc aaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY