| General information | |||
| TC000132 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 10755307..10766623 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000132 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06990 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000132 RA
MKKLFSKIE TKIDNTSKE GNSYIGKVF TVGRQTVTV EDILAEGGF AIVYLVKGT NGTRYALKR MYVNNEQDL NVAKREIQI ASSLSGHKN IIGYVDSSL TATGGGVYE VLLLMPYCQ ENVFGLMKQ RGKANFTEH EVLTIFCDT CEAVSRLHH CKTPIIHRD LKVENILVG ENGNYVICD FGSATAKVL NPADKGVAP VEEEIKRYT TLSYRAPEM VDMYCGKPI TTKADIWAL GCLLYRLCF FTLPFGEST LAIQSGNFS IPDNSKYSK GLHQLMRYM LEPDPDKRP DIFQVSCVA FQLLSKENP VKNLMKSPI PKLEDLPVP PFESELKKA QVKVYNKTP AAPIVEGTS VMPRQRPKG SSTTPLNLN NIPLTLSPS PTTSKKSQS PVTQPPVTT YPSNFQTQP TFEPVKQLD SLFQSSIYP DPFRDDNNA TESPTNEAN NGGAQVKSP ASPENPLFG SGNLNQGAP AKPSLTESS SYVGATMTP PSTPTLAVP KGHRRNMSD TTAFNKVYA SETSQFLAP YESSMKPRS SDSPEMIKG PLVPPGVSA STADINHPI APDSRSLSA DVADWNPFE EPPFSQMTE DHIFGEEFD KIRRGSQSS IQGVKSRES LVMSVSEDP FGCAPFSLP VRSRDRINK TQLQFSVLT QKIKEDDQE LIAYSPPQQ SETEAPLIQ TELVTSPPY VKAPLEDRS KYEKLTQSN YISSESSSD ENNADKVKK KRKTIPEKL QSVYKTVEL PIKFRNEKK TQKKVKHKS DSVDIESDD SIGSASDLR IDEDTEHVK GDAISETVS ESIKTCGSS AYHAECESM ATHEDDSTN RMIRAKIKE EAKKSTVQD EDMLFVGHK YGDKPLLLD DELDSDCEV KFKQWPEKR AATDLWIPP SSSFEDEDV FALAPFNKP RSKSKTEKN SNPFLDFPN PVQSTSSYG TVTVNSNFI NIEIPANFN VTRFESNFT EVDPYFPPP PETKEIIYE NVNIPEKTE PGYDLFDFA PTEVEPVSN VTFSLEASH HKHKKDKKK SKYHLIDES VSDESPSIN FPKSAKIVK PGYRKVAKN KSHKCKTKS QGGFSNMSF EDFSSDDRE EVERSVMMP FEVLRSPEQ EDRKFATKR ISNPFS
- >TC000132 RB
MKKLFSKIE TKIDNTSKE GNSYIGKVF TVGRQTVTV EDILAEGGF AIVYLVKGT NGTRYALKR MYVNNEQDL NVAKREIQI ASSLSGHKN IIGYVDSSL TATGGGVYE VLLLMPYCQ ENVFGLMKQ RGKANFTEH EVLTIFCDT CEAVSRLHH CKTPIIHRD LKVENILVG ENGNYVICD FGSATAKVL NPADKGVAP VEEEIKRYT TLSYRAPEM VDMYCGKPI TTKADIWAL GCLLYRLCF FTLPFGEST LAIQSGNFS IPDNSKYSK GLHQLMRYM LEPDPDKRP DIFQVSCVA FQLLSKENP VKNLMKSPI PKLEDLPVP PFESELKKA QVKVYNKTP AAPIVEGTS VMPRQRPKG SSTTPLNLN NIPLTLSPS PTTSKKSQS PVTQPPVTT YPSNFQTQP TFEPVKQLD SLFQSSIYP DPFRDDNNA TESPTNEAN NGGAQVKSP ASPENPLFG SGNLNQGAP AKPSLTESS SYVGATMTP PSTPTLAVP KGHRRNMSD TTAFNKVYA SETSQFLAP YESSMKPRS SDSPEMIKG PLVPPGVSA STADINHPI APDSRSLSA DVADWNPFE EPPFSQMTE DHIFGEEFD KIRRGSQSS IQGVKSRES LVMSVSEDP FGCAPFSLP VRSRDRINK TQLQFSETP VQYVNVAVE WAK
- >TC000132 RA
atgaaaaaa ctgttttct aaaatcgaa actaaaata gataacact tcgaaggaa ggtaacagt tacatagga aaagtgttc acagtcggc cgccaaacg gtaaccgtc gaggatatt ctagccgaa ggtggtttt gcgatcgtg tatctggta aagggcacc aatggcacg cgctacgca ctcaaacga atgtacgtt aataacgag caggacctt aacgttgcc aaacgtgaa attcaaatc gctagcagt ttaagtggc cataaaaac atcattggt tatgtagat tccagtttg acggccaca ggagggggc gtttatgaa gtattgttg ttgatgcct tattgtcag gaaaacgtt ttcggtctt atgaaacaa cgtggcaaa gcgaatttt acggaacat gaagttctg accattttc tgtgatact tgcgaggcc gtgtcacga ttgcatcac tgcaaaact ccaataatt catcgtgat ttaaaggta gaaaatatt ttggtaggt gaaaatggt aactacgtg atatgtgat tttggttcg gccactgcg aaagtatta aacccggct gataagggg gttgctccc gttgaagaa gagattaaa cggtacaca acactgagt tatagggca cctgagatg gtcgacatg tattgtggc aaaccaatc acgacgaaa gctgatatt tgggctctt ggctgttta ttgtataga ttatgcttt tttacgtta ccgtttggt gaaagtact ttagctata caaagtggg aactttagc attccagat aattcgaaa tattcgaaa ggtttacat cagctaatg cgctacatg ttggagccc gatcctgac aaaaggcct gacattttc caagtcagc tgcgtcgcc tttcaactc ctttcgaaa gaaaacccc gtcaaaaac ctaatgaaa tcgcccatt cccaagctg gaggacctt ccagtgcct ccgttcgag tccgaactg aagaaagct caagtcaaa gtgtacaac aaaacgccg gcagccccc atcgtggaa ggcacgagt gtgatgccc cgccagcgt cccaaagga agctccaca acacctctc aaccttaac aacatccca ctaacccta agtcccagt cctaccacc agtaaaaaa tcacaaagt cccgtaact caaccacct gttaccaca tacccttct aattttcag acccaacca acattcgaa cccgtcaag caattggac tccctcttt cagtcttcc atctatcct gatcccttt cgagacgat aataatgca actgaatcg ccaacaaac gaggctaat aatgggggc gcccaagtc aaatcgcct gcctcaccc gaaaacccc ctatttggt tcggggaat ttgaatcag ggggcaccc gcaaaaccg agtttgact gaatcaagt agttacgtg ggggcgacg atgacgccg ccctctaca ccgaccttg gccgtaccc aaaggacac cggaggaac atgagcgac acgacggcg tttaataag gtttatgcc tcggaaacg tcgcagttt ctggcccct tatgagtcg tccatgaag ccgcgcagc agcgattcg cctgaaatg ataaagggg cctctggtg ccccctggc gtttcggca tcaactgct gatataaac cacccgata gcccccgat agtcgatca ctttcggcc gacgtggct gactggaat cccttcgag gagccccct tttagtcaa atgacggaa gaccacatt ttcggtgaa gaattcgat aagattagg agaggaagt cagagtagt attcaaggt gttaagtca agagaaagt ctagtaatg tcggtgtct gaagatccg ttcggttgt gcaccgttc agtcttcca gtcaggtca cgtgatcgt attaacaag actcagctc cagttctca gttttgacg caaaaaatc aaagaagat gatcaagaa ttgatcgct tattcgccc cctcaacag tccgaaacg gaagcacct ttgattcaa accgaatta gtcacatca ccaccgtac gtcaaagcg ccacttgaa gaccgttcc aagtacgaa aaactaaca cagagtaat tacatcagt tccgaaagt agttcagac gaaaataac gccgataaa gtgaaaaag aagcgtaaa acaataccg gaaaaacta caatcggtt tataaaact gtcgaattg ccgatcaag ttccgcaac gagaaaaaa acacaaaag aaggtgaaa cacaagagt gactctgtc gatattgaa tcggacgat tcgattggt tcggctagt gatttgcga atcgacgaa gacacagaa catgttaaa ggggacgcg attagcgaa acagttagc gaaagtatt aaaacgtgc ggctcgtcg gcttatcac gctgaatgc gaaagcatg gcaacgcac gaagacgac agcactaat cgaatgata agagcgaaa attaaggag gaagcaaaa aagagcaca gttcaagat gaagacatg ctcttcgtt ggtcataaa tacggcgat aagccgttg ctattggac gacgaattg gactcggac tgtgaggtc aagttcaaa caatggcct gaaaaacgc gccgccact gatttgtgg attccccca tccagcagt ttcgaagac gaagatgtt ttcgctttg gcgccgttc aacaaacca cgatccaaa tcaaaaact gagaaaaac tcaaacccg ttcctcgat tttccgaat ccggtccaa agcacgtcg agttacggc acagtgact gtaaattcg aatttcatt aatatcgaa attcccgcc aacttcaac gtgacccga ttcgagtcg aacttcacc gaagtcgac ccctatttc ccgccaccg cccgaaacc aaagaaatt atctacgaa aatgtcaat attcccgaa aagaccgaa ccggggtac gatttgttc gatttcgca cccaccgaa gtcgagcct gtctcgaac gttaccttt agtctagaa gccagccac cacaaacac aagaaagac aagaagaag tccaagtac catttgatc gatgagagc gttagtgac gagagcccc agtattaat tttcccaag agtgcgaaa attgttaag cccggttac cggaaagtg gcgaaaaac aagtcgcac aagtgcaag acgaaatcg caaggtggg tttagtaat atgagtttt gaggatttc tcgtccgat gatcgcgaa gaggtcgaa cggtctgtg atgatgcct tttgaagtg ttgcgtagt cccgaacag gaggatagg aaatttgca acaaaacgg atcagtaat ccgttttct taa
- >TC000132 RB
atgaaaaaa ctgttttct aaaatcgaa actaaaata gataacact tcgaaggaa ggtaacagt tacatagga aaagtgttc acagtcggc cgccaaacg gtaaccgtc gaggatatt ctagccgaa ggtggtttt gcgatcgtg tatctggta aagggcacc aatggcacg cgctacgca ctcaaacga atgtacgtt aataacgag caggacctt aacgttgcc aaacgtgaa attcaaatc gctagcagt ttaagtggc cataaaaac atcattggt tatgtagat tccagtttg acggccaca ggagggggc gtttatgaa gtattgttg ttgatgcct tattgtcag gaaaacgtt ttcggtctt atgaaacaa cgtggcaaa gcgaatttt acggaacat gaagttctg accattttc tgtgatact tgcgaggcc gtgtcacga ttgcatcac tgcaaaact ccaataatt catcgtgat ttaaaggta gaaaatatt ttggtaggt gaaaatggt aactacgtg atatgtgat tttggttcg gccactgcg aaagtatta aacccggct gataagggg gttgctccc gttgaagaa gagattaaa cggtacaca acactgagt tatagggca cctgagatg gtcgacatg tattgtggc aaaccaatc acgacgaaa gctgatatt tgggctctt ggctgttta ttgtataga ttatgcttt tttacgtta ccgtttggt gaaagtact ttagctata caaagtggg aactttagc attccagat aattcgaaa tattcgaaa ggtttacat cagctaatg cgctacatg ttggagccc gatcctgac aaaaggcct gacattttc caagtcagc tgcgtcgcc tttcaactc ctttcgaaa gaaaacccc gtcaaaaac ctaatgaaa tcgcccatt cccaagctg gaggacctt ccagtgcct ccgttcgag tccgaactg aagaaagct caagtcaaa gtgtacaac aaaacgccg gcagccccc atcgtggaa ggcacgagt gtgatgccc cgccagcgt cccaaagga agctccaca acacctctc aaccttaac aacatccca ctaacccta agtcccagt cctaccacc agtaaaaaa tcacaaagt cccgtaact caaccacct gttaccaca tacccttct aattttcag acccaacca acattcgaa cccgtcaag caattggac tccctcttt cagtcttcc atctatcct gatcccttt cgagacgat aataatgca actgaatcg ccaacaaac gaggctaat aatgggggc gcccaagtc aaatcgcct gcctcaccc gaaaacccc ctatttggt tcggggaat ttgaatcag ggggcaccc gcaaaaccg agtttgact gaatcaagt agttacgtg ggggcgacg atgacgccg ccctctaca ccgaccttg gccgtaccc aaaggacac cggaggaac atgagcgac acgacggcg tttaataag gtttatgcc tcggaaacg tcgcagttt ctggcccct tatgagtcg tccatgaag ccgcgcagc agcgattcg cctgaaatg ataaagggg cctctggtg ccccctggc gtttcggca tcaactgct gatataaac cacccgata gcccccgat agtcgatca ctttcggcc gacgtggct gactggaat cccttcgag gagccccct tttagtcaa atgacggaa gaccacatt ttcggtgaa gaattcgat aagattagg agaggaagt cagagtagt attcaaggt gttaagtca agagaaagt ctagtaatg tcggtgtct gaagatccg ttcggttgt gcaccgttc agtcttcca gtcaggtca cgtgatcgt attaacaag actcagctc cagttctca gagacgcct gtacaatat gtaaacgtt gctgttgaa tgggccaag tag
- >TC000132 RA
actttctta aacgtcact gacactgtt atgcggaca attatttta atcaattaa taaaaatag tcgatttga agcggtagc aagtgtgag gtgggctgt cgtaacggt ctggagcca gaaaattga tggggaatc cgcattcac tcaccaaac tcaatgtgt cataatttc caattaagt gtcaatcac gatgaaaaa actgttttc taaaatcga aactaaaat agataacac ttcgaagga aggtaacag ttacatagg aaaagtgtt cacagtcgg ccgccaaac ggtaaccgt cgaggatat tctagccga aggtggttt tgcgatcgt gtatctggt aaagggcac caatggcac gcgctacgc actcaaacg aatgtacgt taataacga gcaggacct taacgttgc caaacgtga aattcaaat cgctagcag tttaagtgg ccataaaaa catcattgg ttatgtaga ttccagttt gacggccac aggaggggg cgtttatga agtattgtt gttgatgcc ttattgtca ggaaaacgt tttcggtct tatgaaaca acgtggcaa agcgaattt tacggaaca tgaagttct gaccatttt ctgtgatac ttgcgaggc cgtgtcacg attgcatca ctgcaaaac tccaataat tcatcgtga tttaaaggt agaaaatat tttggtagg tgaaaatgg taactacgt gatatgtga ttttggttc ggccactgc gaaagtatt aaacccggc tgataaggg ggttgctcc cgttgaaga agagattaa acggtacac aacactgag ttatagggc acctgagat ggtcgacat gtattgtgg caaaccaat cacgacgaa agctgatat ttgggctct tggctgttt attgtatag attatgctt ttttacgtt accgtttgg tgaaagtac tttagctat acaaagtgg gaactttag cattccaga taattcgaa atattcgaa aggtttaca tcagctaat gcgctacat gttggagcc cgatcctga caaaaggcc tgacatttt ccaagtcag ctgcgtcgc ctttcaact cctttcgaa agaaaaccc cgtcaaaaa cctaatgaa atcgcccat tcccaagct ggaggacct tccagtgcc tccgttcga gtccgaact gaagaaagc tcaagtcaa agtgtacaa caaaacgcc ggcagcccc catcgtgga aggcacgag tgtgatgcc ccgccagcg tcccaaagg aagctccac aacacctct caaccttaa caacatccc actaaccct aagtcccag tcctaccac cagtaaaaa atcacaaag tcccgtaac tcaaccacc tgttaccac atacccttc taattttca gacccaacc aacattcga acccgtcaa gcaattgga ctccctctt tcagtcttc catctatcc tgatccctt tcgagacga taataatgc aactgaatc gccaacaaa cgaggctaa taatggggg cgcccaagt caaatcgcc tgcctcacc cgaaaaccc cctatttgg ttcggggaa tttgaatca gggggcacc cgcaaaacc gagtttgac tgaatcaag tagttacgt gggggcgac gatgacgcc gccctctac accgacctt ggccgtacc caaaggaca ccggaggaa catgagcga cacgacggc gtttaataa ggtttatgc ctcggaaac gtcgcagtt tctggcccc ttatgagtc gtccatgaa gccgcgcag cagcgattc gcctgaaat gataaaggg gcctctggt gccccctgg cgtttcggc atcaactgc tgatataaa ccacccgat agcccccga tagtcgatc actttcggc cgacgtggc tgactggaa tcccttcga ggagccccc ttttagtca aatgacgga agaccacat tttcggtga agaattcga taagattag gagaggaag tcagagtag tattcaagg tgttaagtc aagagaaag tctagtaat gtcggtgtc tgaagatcc gttcggttg tgcaccgtt cagtcttcc agtcaggtc acgtgatcg tattaacaa gactcagct ccagttctc agttttgac gcaaaaaat caaagaaga tgatcaaga attgatcgc ttattcgcc ccctcaaca gtccgaaac ggaagcacc tttgattca aaccgaatt agtcacatc accaccgta cgtcaaagc gccacttga agaccgttc caagtacga aaaactaac acagagtaa ttacatcag ttccgaaag tagttcaga cgaaaataa cgccgataa agtgaaaaa gaagcgtaa aacaatacc ggaaaaact acaatcggt ttataaaac tgtcgaatt gccgatcaa gttccgcaa cgagaaaaa aacacaaaa gaaggtgaa acacaagag tgactctgt cgatattga atcggacga ttcgattgg ttcggctag tgatttgcg aatcgacga agacacaga acatgttaa aggggacgc gattagcga aacagttag cgaaagtat taaaacgtg cggctcgtc ggcttatca cgctgaatg cgaaagcat ggcaacgca cgaagacga cagcactaa tcgaatgat aagagcgaa aattaagga ggaagcaaa aaagagcac agttcaaga tgaagacat gctcttcgt tggtcataa atacggcga taagccgtt gctattgga cgacgaatt ggactcgga ctgtgaggt caagttcaa acaatggcc tgaaaaacg cgccgccac tgatttgtg gattccccc atccagcag tttcgaaga cgaagatgt tttcgcttt ggcgccgtt caacaaacc acgatccaa atcaaaaac tgagaaaaa ctcaaaccc gttcctcga ttttccgaa tccggtcca aagcacgtc gagttacgg cacagtgac tgtaaattc gaatttcat taatatcga aattcccgc caacttcaa cgtgacccg attcgagtc gaacttcac cgaagtcga cccctattt cccgccacc gcccgaaac caaagaaat tatctacga aaatgtcaa tattcccga aaagaccga accggggta cgatttgtt cgatttcgc acccaccga agtcgagcc tgtctcgaa cgttacctt tagtctaga agccagcca ccacaaaca caagaaaga caagaagaa gtccaagta ccatttgat cgatgagag cgttagtga cgagagccc cagtattaa ttttcccaa gagtgcgaa aattgttaa gcccggtta ccggaaagt ggcgaaaaa caagtcgca caagtgcaa gacgaaatc gcaaggtgg gtttagtaa tatgagttt tgaggattt ctcgtccga tgatcgcga agaggtcga acggtctgt gatgatgcc ttttgaagt gttgcgtag tcccgaaca ggaggatag gaaatttgc aacaaaacg gatcagtaa tccgttttc ttaagcagc tttgtgacg taaggcagc tgtggtttt gttactatt tattcaaat tcaagctta tgcgtggta aggccacga tttatttat tcaaactga tgatattgg catacctgg ttgggggtc cccttatta acttgtgtg acatttatc aaatatggc gttaggact ttttccacg gcactgcga tttgggtaa aaattttga gcacgttta cacgccgtt ttgtgatcg ttattaaaa gaccaaatc taggctttg tgctttgat acttgatgt taggggtcg tacaatgaa taaataaca catttacta tcacatttc taaacagaa atttcttta tttccaaca ttttaataa caacgctgt gtaaacgta gtcataaat cacgattgc cgtgaatga cgacatttt gttaaatgt caaaaattt aagtgatgg tgattgtgt ccaggtatg ttaatttat tttttgtgt tagtagttg aagacaaga ctgtaatta ttacgttac ttaagcatt atttaaagt ctgagtaaa actgcgtca agtgtttcg gtgcctttt ccaatcaaa atttgcaaa agaataacg aatgaaaac gctaccaat ccgttgata ataataagc caaagtgta agcaaactt gaatttcct acatatagc aagtcaatt aaaacgctt ttattgtga catctaggt cctgtttct agtgtataa gatatgaaa cctagttga tgtttaaaa gattattta tttataaaa aaagaagtg tatacattg ttcgtataa aaacattcc atttattta tatttgtag tactattta aatattaat tgcactttt gtaacttgt aatatttta taaaacgca tacaaataa atttgttgt cttaaagtc attgttacg ttacaaata cggaggact aaagcgtag gacacgaaa cgagataca aaagcgcac gatattctg tacagaaaa aatatagta cgatagaat aaaaatgag ggcgctgca agcgccaaa taatcgttt taaattaat ttaaattca acaaattca ctaataact tcgaagaaa atacagaaa tgttgagct ttacattct taatagacc gagtaaatc taactgcta aaattttca caagaaaca attgaaaat agtatatta aaaaacaag ttttataag cacaaattc agtttcaaa aacgagtgg cgttattca cgagttatt tgaaagaac atgtgcttc aaggtctaa tgaaacgac ctctttata ctgtttata ccctttttt ccgttttta aacagaaat ttttatttt aattgattt agggatttt tctgacgat tagtaatga aagtgaaaa acgtatttt aaatttttg ttttatccc aattgggtc aaaaaaaca ggagtatgg atgataata aagatactc ttgcaaa
- >TC000132 RB
actttctta aacgtcact gacactgtt atgcggaca attatttta atcaattaa taaaaatag tcgatttga agcggtagc aagtgtgag gtgggctgt cgtaacggt ctggagcca gaaaattga tggggaatc cgcattcac tcaccaaac tcaatgtgt cataatttc caattaagt gtcaatcac gatgaaaaa actgttttc taaaatcga aactaaaat agataacac ttcgaagga aggtaacag ttacatagg aaaagtgtt cacagtcgg ccgccaaac ggtaaccgt cgaggatat tctagccga aggtggttt tgcgatcgt gtatctggt aaagggcac caatggcac gcgctacgc actcaaacg aatgtacgt taataacga gcaggacct taacgttgc caaacgtga aattcaaat cgctagcag tttaagtgg ccataaaaa catcattgg ttatgtaga ttccagttt gacggccac aggaggggg cgtttatga agtattgtt gttgatgcc ttattgtca ggaaaacgt tttcggtct tatgaaaca acgtggcaa agcgaattt tacggaaca tgaagttct gaccatttt ctgtgatac ttgcgaggc cgtgtcacg attgcatca ctgcaaaac tccaataat tcatcgtga tttaaaggt agaaaatat tttggtagg tgaaaatgg taactacgt gatatgtga ttttggttc ggccactgc gaaagtatt aaacccggc tgataaggg ggttgctcc cgttgaaga agagattaa acggtacac aacactgag ttatagggc acctgagat ggtcgacat gtattgtgg caaaccaat cacgacgaa agctgatat ttgggctct tggctgttt attgtatag attatgctt ttttacgtt accgtttgg tgaaagtac tttagctat acaaagtgg gaactttag cattccaga taattcgaa atattcgaa aggtttaca tcagctaat gcgctacat gttggagcc cgatcctga caaaaggcc tgacatttt ccaagtcag ctgcgtcgc ctttcaact cctttcgaa agaaaaccc cgtcaaaaa cctaatgaa atcgcccat tcccaagct ggaggacct tccagtgcc tccgttcga gtccgaact gaagaaagc tcaagtcaa agtgtacaa caaaacgcc ggcagcccc catcgtgga aggcacgag tgtgatgcc ccgccagcg tcccaaagg aagctccac aacacctct caaccttaa caacatccc actaaccct aagtcccag tcctaccac cagtaaaaa atcacaaag tcccgtaac tcaaccacc tgttaccac atacccttc taattttca gacccaacc aacattcga acccgtcaa gcaattgga ctccctctt tcagtcttc catctatcc tgatccctt tcgagacga taataatgc aactgaatc gccaacaaa cgaggctaa taatggggg cgcccaagt caaatcgcc tgcctcacc cgaaaaccc cctatttgg ttcggggaa tttgaatca gggggcacc cgcaaaacc gagtttgac tgaatcaag tagttacgt gggggcgac gatgacgcc gccctctac accgacctt ggccgtacc caaaggaca ccggaggaa catgagcga cacgacggc gtttaataa ggtttatgc ctcggaaac gtcgcagtt tctggcccc ttatgagtc gtccatgaa gccgcgcag cagcgattc gcctgaaat gataaaggg gcctctggt gccccctgg cgtttcggc atcaactgc tgatataaa ccacccgat agcccccga tagtcgatc actttcggc cgacgtggc tgactggaa tcccttcga ggagccccc ttttagtca aatgacgga agaccacat tttcggtga agaattcga taagattag gagaggaag tcagagtag tattcaagg tgttaagtc aagagaaag tctagtaat gtcggtgtc tgaagatcc gttcggttg tgcaccgtt cagtcttcc agtcaggtc acgtgatcg tattaacaa gactcagct ccagttctc agagacgcc tgtacaata tgtaaacgt tgctgttga atgggccaa gtagtcagt gtttaaaaa aacaattaa aacaaagtt tgaaatgaa aaaattagt gaaatttca cagtagcac agccgcttt gatggctag aaaacgttc atagatggc tgacagtaa gctgtaagg tacaatttt attcgttgt aatgacgtt tttaaggtc attagttgg ctgcgaccg gccgtgtag gaacgcagt tcctattca tcaaagtgg ctgtgagaa gtaggctta gataataac tagtgtact tgataataa taatgcgtg atggcaatg ttgcttcga ttgttttca tcgcaggcc aatattttt tccctatat ctggcctgc aatttctat aagatcccc ttgtttaaa tgttcaaaa tatccaaaa caatgtgat aatcactaa tgagaacat aatctttat aatacttgt gttatgtgg accaatatt gagtttttt catttatta ttagagaag taagtcgtc cgtgtgcaa tagtttgta tgtcgtact taagataga taggagaga aaacgatgg ctaaatttt ttatcattc caaatgaaa gttgaaaaa aaaatacag acggattcc aaag
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