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Gene information
General information
TC000063 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10980922..10983386
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000063
    iBeetle screen iB_06983

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000063 RA
      MTNIAGDLL KCVNFAAEK HKDQRRKNA SKTPYINHP 
IGVAYILTH EAEIDDLDV LQAALLHDT VEDTATTFE 
EIEQVFGKR VRDIVEEVT DDKSLPKEE RKRLQIEHA 
PGASHQAKL VKLADKLYN LRDLENSAP EGWSEQRIQ 
EYFTWSSKV VAGLRGTNP ALERSLDQQ SLCQTRPAY 
RQGRRLTAV KTYTIANES RHLFVYGVP KIDLFSELK 
ALCAKQGKL AKFHLVADH KTEIFTECF HVVYDKFEV 
ARKAKRLLD NKSFYGGIL HVCYAPEYE SVDETRAKL 
LLRRQSILD YKG
    CDS sequence
    • >TC000063 RA
      atgacaaac attgccggg gatttacta aagtgtgtc 
aacttcgcc gccgagaaa cacaaagac cagcgacgc 
aagaacgcc tcaaagacg ccctacatc aaccacccc 
attggagtg gcctacatc ctcacacac gaggccgaa 
atcgacgat cttgacgtg ctgcaagcc gctctcttg 
cacgacact gtcgaagac acagccacg actttcgag 
gagatcgag caagtgttc gggaagcga gtccgtgac 
attgttgag gaggtaacc gatgataaa agtctgccg 
aaggaagag cgcaaaaga ctgcaaatc gagcacgcc 
ccaggtgcc tcacaccag gccaaactc gtgaaactc 
gccgacaaa ttgtacaat ttgcgagac ttggagaac 
tctgccccg gaaggctgg agcgagcaa cggatccag 
gagtacttc acctggagc agcaaagtc gtggcgggg 
ctgaggggc acgaacccg gccctggaa cgcagcctc 
gaccagcaa agtctttgc caaaccagg cctgcttac 
agacaaggc cggcgactt actgctgtg aaaacttac 
accatcgcg aacgaatcg cgacattta ttcgtctac 
ggtgtccct aaaatcgat cttttcagc gaactcaaa 
gccctctgt gccaaacag gggaagtta gcaaagttt 
catctagtc gcagaccac aaaactgag attttcacc 
gaatgtttc cacgtggtc tacgacaag tttgaagtg 
gcaaggaag gccaaaaga ctgcttgat aataagtcg 
ttctatgga gggatcctc cacgtgtgc tacgccccc 
gagtacgaa tctgtggac gaaactagg gccaagttg 
ttgttaagg aggcaaagt attttagat tataaagga 
taa
    mRNA sequence
    • >TC000063 RA
      gaccgtgat gacaaacat tgccgggga tttactaaa 
gtgtgtcaa cttcgccgc cgagaaaca caaagacca 
gcgacgcaa gaacgcctc aaagacgcc ctacatcaa 
ccaccccat tggagtggc ctacatcct cacacacga 
ggccgaaat cgacgatct tgacgtgct gcaagccgc 
tctcttgca cgacactgt cgaagacac agccacgac 
tttcgagga gatcgagca agtgttcgg gaagcgagt 
ccgtgacat tgttgagga ggtaaccga tgataaaag 
tctgccgaa ggaagagcg caaaagact gcaaatcga 
gcacgcccc aggtgcctc acaccaggc caaactcgt 
gaaactcgc cgacaaatt gtacaattt gcgagactt 
ggagaactc tgccccgga aggctggag cgagcaacg 
gatccagga gtacttcac ctggagcag caaagtcgt 
ggcggggct gaggggcac gaacccggc cctggaacg 
cagcctcga ccagcaaag tctttgcca aaccaggcc 
tgcttacag acaaggccg gcgacttac tgctgtgaa 
aacttacac catcgcgaa cgaatcgcg acatttatt 
cgtctacgg tgtccctaa aatcgatct tttcagcga 
actcaaagc cctctgtgc caaacaggg gaagttagc 
aaagtttca tctagtcgc agaccacaa aactgagat 
tttcaccga atgtttcca cgtggtcta cgacaagtt 
tgaagtggc aaggaaggc caaaagact gcttgataa 
taagtcgtt ctatggagg gatcctcca cgtgtgcta 
cgcccccga gtacgaatc tgtggacga aactagggc 
caagttgtt gttaaggag gcaaagtat tttagatta 
taaaggata agttattat tttcaactg ttttttttt 
atttcccta tgacaaaaa attagagag actgaataa 
taaatcata aaatataaa tcgcataat tgcacaata 
aaataaatg actagttaa aatacagtc agcgaatta 
taagttgcc aataactat acttgaatg agtagttgt 
caggcaata aacaataaa aacagtgcg aatttcgtg 
gccttctgc aaaaaatga ccaagtcaa aattaattc 
agtaggctg aatttggcc acgtgatta gttaatagg 
gtgtttgat tgccagatt ggtgattta ataactctt 
ttatcaacc agccctgaa agttgtttt ctgtaaata 
cacttttag tgagaaatt taataaaac aaaaaacat 
ttatgtgtt gtgggaatc tgtatgttt atagttttc 
caaaaaata tggttatac taatgtcca ccgttacaa 
tatttgtgc tgtacaaag ctatgtctt taaatacat 
cgttcttca cgtcaggtc aggtcaggt caaaaatga 
tcactttga aaatcaatt taagtttcc aaaataata 
aaaaattat agtttaaat tacaaatat gtactattc 
atccctaaa attaaaata aaatgtgta cagttttta 
gtagaaaaa caattacag gtatcgaat taacttttt 
caattaaat taagtaaac tacggtgtt atgtatttt 
ttattttat taaaggtag acaatgcat aattttatt 
gtctgttcc tttttgatt tatggacga gtaaaagaa 
ttttttcct aaagtttaa ctataccag tttttatct 
cttctaaaa agacgatac aatggtctt attcgaaat 
tgttttgtt catttcaaa tatttttta aaacatgga 
accatagga aatttttag taataatat tatttcttt 
tggaattac gactataga ctgtggagt tttttgctt 
taatcttac aagtttggt tacttaaaa aattatagt 
caacgagtg aaaatgagc ctgaataaa tttgaaacg 
caactaaac atgcatttt cattacttt ttaaattct 
tgttttaat ttttttata aaaaaatat aataagtaa 
tgcacaaat ttgacggaa agttcaaaa tttgtcagt 
ttctgtcat acctaagta ttaaaaaaa tatatttga 
atgacgaaa agatagata tgctcttaa tttacggtg 
agtgccaaa aaacccgtc tttattaac aatgttttc 
ctgaaagaa aagcacctg catacccca tttttaaaa 
gactagaaa aagtttctt cagagaaaa ctggccatc 
tgcgaaaaa aaaactcga ggacgaaca gctaccgat 
gttaacaat tgaatggaa tagtgtgga ccatttaac 
tccaaaaca ataatgcgt gttaaaaca tccttttac 
caaacacgt gacagaaac gagggacaa aactgcatt 
tattgcatt tattataaa taaaaaatg tttgattct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY