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Gene information
General information
TC000063 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10980922..10983386
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000063
    iBeetle screen iB_06983

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000063 RA
      MTNIAGDLL KCVNFAAEK HKDQRRKNA SKTPYINHP 
      IGVAYILTH EAEIDDLDV LQAALLHDT VEDTATTFE 
      EIEQVFGKR VRDIVEEVT DDKSLPKEE RKRLQIEHA 
      PGASHQAKL VKLADKLYN LRDLENSAP EGWSEQRIQ 
      EYFTWSSKV VAGLRGTNP ALERSLDQQ SLCQTRPAY 
      RQGRRLTAV KTYTIANES RHLFVYGVP KIDLFSELK 
      ALCAKQGKL AKFHLVADH KTEIFTECF HVVYDKFEV 
      ARKAKRLLD NKSFYGGIL HVCYAPEYE SVDETRAKL 
      LLRRQSILD YKG
    CDS sequence
    • >TC000063 RA
      atgacaaac attgccggg gatttacta aagtgtgtc 
      aacttcgcc gccgagaaa cacaaagac cagcgacgc 
      aagaacgcc tcaaagacg ccctacatc aaccacccc 
      attggagtg gcctacatc ctcacacac gaggccgaa 
      atcgacgat cttgacgtg ctgcaagcc gctctcttg 
      cacgacact gtcgaagac acagccacg actttcgag 
      gagatcgag caagtgttc gggaagcga gtccgtgac 
      attgttgag gaggtaacc gatgataaa agtctgccg 
      aaggaagag cgcaaaaga ctgcaaatc gagcacgcc 
      ccaggtgcc tcacaccag gccaaactc gtgaaactc 
      gccgacaaa ttgtacaat ttgcgagac ttggagaac 
      tctgccccg gaaggctgg agcgagcaa cggatccag 
      gagtacttc acctggagc agcaaagtc gtggcgggg 
      ctgaggggc acgaacccg gccctggaa cgcagcctc 
      gaccagcaa agtctttgc caaaccagg cctgcttac 
      agacaaggc cggcgactt actgctgtg aaaacttac 
      accatcgcg aacgaatcg cgacattta ttcgtctac 
      ggtgtccct aaaatcgat cttttcagc gaactcaaa 
      gccctctgt gccaaacag gggaagtta gcaaagttt 
      catctagtc gcagaccac aaaactgag attttcacc 
      gaatgtttc cacgtggtc tacgacaag tttgaagtg 
      gcaaggaag gccaaaaga ctgcttgat aataagtcg 
      ttctatgga gggatcctc cacgtgtgc tacgccccc 
      gagtacgaa tctgtggac gaaactagg gccaagttg 
      ttgttaagg aggcaaagt attttagat tataaagga 
      taa
    mRNA sequence
    • >TC000063 RA
      gaccgtgat gacaaacat tgccgggga tttactaaa 
      gtgtgtcaa cttcgccgc cgagaaaca caaagacca 
      gcgacgcaa gaacgcctc aaagacgcc ctacatcaa 
      ccaccccat tggagtggc ctacatcct cacacacga 
      ggccgaaat cgacgatct tgacgtgct gcaagccgc 
      tctcttgca cgacactgt cgaagacac agccacgac 
      tttcgagga gatcgagca agtgttcgg gaagcgagt 
      ccgtgacat tgttgagga ggtaaccga tgataaaag 
      tctgccgaa ggaagagcg caaaagact gcaaatcga 
      gcacgcccc aggtgcctc acaccaggc caaactcgt 
      gaaactcgc cgacaaatt gtacaattt gcgagactt 
      ggagaactc tgccccgga aggctggag cgagcaacg 
      gatccagga gtacttcac ctggagcag caaagtcgt 
      ggcggggct gaggggcac gaacccggc cctggaacg 
      cagcctcga ccagcaaag tctttgcca aaccaggcc 
      tgcttacag acaaggccg gcgacttac tgctgtgaa 
      aacttacac catcgcgaa cgaatcgcg acatttatt 
      cgtctacgg tgtccctaa aatcgatct tttcagcga 
      actcaaagc cctctgtgc caaacaggg gaagttagc 
      aaagtttca tctagtcgc agaccacaa aactgagat 
      tttcaccga atgtttcca cgtggtcta cgacaagtt 
      tgaagtggc aaggaaggc caaaagact gcttgataa 
      taagtcgtt ctatggagg gatcctcca cgtgtgcta 
      cgcccccga gtacgaatc tgtggacga aactagggc 
      caagttgtt gttaaggag gcaaagtat tttagatta 
      taaaggata agttattat tttcaactg ttttttttt 
      atttcccta tgacaaaaa attagagag actgaataa 
      taaatcata aaatataaa tcgcataat tgcacaata 
      aaataaatg actagttaa aatacagtc agcgaatta 
      taagttgcc aataactat acttgaatg agtagttgt 
      caggcaata aacaataaa aacagtgcg aatttcgtg 
      gccttctgc aaaaaatga ccaagtcaa aattaattc 
      agtaggctg aatttggcc acgtgatta gttaatagg 
      gtgtttgat tgccagatt ggtgattta ataactctt 
      ttatcaacc agccctgaa agttgtttt ctgtaaata 
      cacttttag tgagaaatt taataaaac aaaaaacat 
      ttatgtgtt gtgggaatc tgtatgttt atagttttc 
      caaaaaata tggttatac taatgtcca ccgttacaa 
      tatttgtgc tgtacaaag ctatgtctt taaatacat 
      cgttcttca cgtcaggtc aggtcaggt caaaaatga 
      tcactttga aaatcaatt taagtttcc aaaataata 
      aaaaattat agtttaaat tacaaatat gtactattc 
      atccctaaa attaaaata aaatgtgta cagttttta 
      gtagaaaaa caattacag gtatcgaat taacttttt 
      caattaaat taagtaaac tacggtgtt atgtatttt 
      ttattttat taaaggtag acaatgcat aattttatt 
      gtctgttcc tttttgatt tatggacga gtaaaagaa 
      ttttttcct aaagtttaa ctataccag tttttatct 
      cttctaaaa agacgatac aatggtctt attcgaaat 
      tgttttgtt catttcaaa tatttttta aaacatgga 
      accatagga aatttttag taataatat tatttcttt 
      tggaattac gactataga ctgtggagt tttttgctt 
      taatcttac aagtttggt tacttaaaa aattatagt 
      caacgagtg aaaatgagc ctgaataaa tttgaaacg 
      caactaaac atgcatttt cattacttt ttaaattct 
      tgttttaat ttttttata aaaaaatat aataagtaa 
      tgcacaaat ttgacggaa agttcaaaa tttgtcagt 
      ttctgtcat acctaagta ttaaaaaaa tatatttga 
      atgacgaaa agatagata tgctcttaa tttacggtg 
      agtgccaaa aaacccgtc tttattaac aatgttttc 
      ctgaaagaa aagcacctg catacccca tttttaaaa 
      gactagaaa aagtttctt cagagaaaa ctggccatc 
      tgcgaaaaa aaaactcga ggacgaaca gctaccgat 
      gttaacaat tgaatggaa tagtgtgga ccatttaac 
      tccaaaaca ataatgcgt gttaaaaca tccttttac 
      caaacacgt gacagaaac gagggacaa aactgcatt 
      tattgcatt tattataaa taaaaaatg tttgattct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY