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Gene information
General information
TC000104 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11112168..11115482
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000104
    iBeetle screen iB_06980

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000104 RA
      MSDWDTVTV LKKRAPKAS TMKSEQAVN 
      AARRQGVPV DTQLKWGAG GNKQHVATK 
      NTAKLDRET EELKHETIS LDVGRIIQQ 
      GRQAKGLSQ KDLATKINE KPQVITDYE 
      AGRGIPNNV IIGKIEKVI GLKLRGLPK 
      NQ
    • >TC000104 RB
      MSDWDTVTV LKKRAPKAS TMKSEQAVN 
      AARRQGVPV DTQLKWGAG GNKQHVATK 
      NTAKLDRET EELKHETIS LDVGRIIQQ 
      GRQAKGLSQ KDLATKINE KPQVITDYE 
      AGRGIPNNV IIGKIEKVI GLKLRGKDR 
      GKPLLPPGQ KK
    CDS sequence
    • >TC000104 RA
      atgtcagac tgggacaca gtgaccgtt ttaaaaaag 
      cgggccccc aaagcctcc acaatgaaa agcgagcag 
      gccgtgaat gcggcacgc cgccaaggc gtccccgtc 
      gatacccag ctcaagtgg ggcgccggg ggcaacaag 
      cagcacgtg gccaccaaa aacacagcg aaactcgac 
      cgtgagact gaagaactc aaacacgag accatctcg 
      ttggacgtg ggcaggatt atccagcag ggccgacaa 
      gccaagggg ctcagtcag aaggacctg gccaccaaa 
      atcaacgag aagccgcaa gtcatcacg gactacgag 
      gccggacgg gggatcccc aacaatgtc atcatcggc 
      aagatcgaa aaagtgatt ggcttgaaa ttgagaggt 
      ctaccgaaa aatcaatag
    • >TC000104 RB
      atgtcagac tgggacaca gtgaccgtt ttaaaaaag 
      cgggccccc aaagcctcc acaatgaaa agcgagcag 
      gccgtgaat gcggcacgc cgccaaggc gtccccgtc 
      gatacccag ctcaagtgg ggcgccggg ggcaacaag 
      cagcacgtg gccaccaaa aacacagcg aaactcgac 
      cgtgagact gaagaactc aaacacgag accatctcg 
      ttggacgtg ggcaggatt atccagcag ggccgacaa 
      gccaagggg ctcagtcag aaggacctg gccaccaaa 
      atcaacgag aagccgcaa gtcatcacg gactacgag 
      gccggacgg gggatcccc aacaatgtc atcatcggc 
      aagatcgaa aaagtgatt ggcttgaaa ttgagaggt 
      aaagaccgc ggaaaacca ctcctaccc ccgggacaa 
      aagaagtaa
    mRNA sequence
    • >TC000104 RA
      aacaactac atttagcaa ttatttgca aaataacag 
      caacagcac cgactattt tttcgaaat ttcgtgtga 
      ctatcttct tgaaagtgc caacgtaag atattcaca 
      cttgtcatt tttcaatgt ttacaaaat tctacaaac 
      tgtgcaagg gacatgaaa gacaaaaat taaatatca 
      gggggcgaa aaaattgtg aaccggtat aaccactta 
      accagcctg aagaccaat cagggccgc caaccgccc 
      caacttcac gccagagtt gtttcaatt tcctgaact 
      taattccgc cgaattaca aagtgtcgc cattatctg 
      caaaattgt gatcttttg agtgctttc cgcctcaca 
      tcacgcact ttcgtcgca aaattgtgc aatgttatt 
      gttgccttg acccgtgcc taacttcac agttgtgtc 
      ataaattcg tcgttagtt gtccgagtg tcattcctc 
      aaaatcaaa aatctcacc atgtcagac tgggacaca 
      gtgaccgtt ttaaaaaag cgggccccc aaagcctcc 
      acaatgaaa agcgagcag gccgtgaat gcggcacgc 
      cgccaaggc gtccccgtc gatacccag ctcaagtgg 
      ggcgccggg ggcaacaag cagcacgtg gccaccaaa 
      aacacagcg aaactcgac cgtgagact gaagaactc 
      aaacacgag accatctcg ttggacgtg ggcaggatt 
      atccagcag ggccgacaa gccaagggg ctcagtcag 
      aaggacctg gccaccaaa atcaacgag aagccgcaa 
      gtcatcacg gactacgag gccggacgg gggatcccc 
      aacaatgtc atcatcggc aagatcgaa aaagtgatt 
      ggcttgaaa ttgagaggt ctaccgaaa aatcaatag 
      gtctggcaa cactagtta ggtggtaat aaccatggc 
      aaccaattg ttgactaac agatggcgc cacgttaag 
      ccgtgtccc tcattgatc aaggaagcc gtggttcaa 
      tcatcacgt caaagtaat aattcgaat gtttagata 
      tttgagtgt gacctattt gttataatt ttttagatt 
      attgtatgt ggtagaatt agaattcct ttaccgaaa 
      tatttgtta aataaaa
    • >TC000104 RB
      aacaactac atttagcaa ttatttgca aaataacag 
      caacagcac cgactattt tttcgaaat ttcgtgtga 
      ctatcttct tgaaagtgc caacgtaag atattcaca 
      cttgtcatt tttcaatgt ttacaaaat tctacaaac 
      tgtgcaagg gacatgaaa gacaaaaat taaatatca 
      gggggcgaa aaaattgtg aaccggtat aaccactta 
      accagcctg aagaccaat cagggccgc caaccgccc 
      caacttcac gccagagtt gtttcaatt tcctgaact 
      taattccgc cgaattaca aagtgtcgc cattatctg 
      caaaattgt gatcttttg agtgctttc cgcctcaca 
      tcacgcact ttcgtcgca aaattgtgc aatgttatt 
      gttgccttg acccgtgcc taacttcac agttgtgtc 
      ataaattcg tcgttagtt gtccgagtg tcattcctc 
      aaaatcaaa aatctcacc atgtcagac tgggacaca 
      gtgaccgtt ttaaaaaag cgggccccc aaagcctcc 
      acaatgaaa agcgagcag gccgtgaat gcggcacgc 
      cgccaaggc gtccccgtc gatacccag ctcaagtgg 
      ggcgccggg ggcaacaag cagcacgtg gccaccaaa 
      aacacagcg aaactcgac cgtgagact gaagaactc 
      aaacacgag accatctcg ttggacgtg ggcaggatt 
      atccagcag ggccgacaa gccaagggg ctcagtcag 
      aaggacctg gccaccaaa atcaacgag aagccgcaa 
      gtcatcacg gactacgag gccggacgg gggatcccc 
      aacaatgtc atcatcggc aagatcgaa aaagtgatt 
      ggcttgaaa ttgagaggt aaagaccgc ggaaaacca 
      ctcctaccc ccgggacaa aagaagtaa tgtcctggg 
      gggatggcc agtttttta taattgcga taagtgtca 
      cgaggggct aggaagtgt taaatttag ggatttatg 
      tactgaacc cttcaagtt gttctattc aaattttat 
      tacactaca ataataaag gagttacat ggagcgatt 
      cctcatttc agaaccagt tttcatttc acacttttt 
      cacaaacta aacatgaaa tttttcgtt attacgcac 
      taaaaccct actaattcc aagtgtgcg aaaaatcga 
      ctattttca cacattttg tagacaact gtatttttt 
      actttttta tgtatcttc cttgatccg tgcaggaag 
      gagccactt agagttaga gacacttct gtcagttac 
      tatcagtgt tgccagatg cattgtctc gtttttttc 
      aacactcgt ataaaaata aaaagcaag agtgtgtat 
      atagattta taaaattag ttacagatg aataattaa 
      aaaatattg gtctcaaaa atataaaat aatcacaaa 
      attcaacaa acaacagtt ttaacttgt acatgtctt 
      attctttga ctgaattga tttttgata atttttttc 
      gccaaccaa acttttggt atacagcaa tccagaaat 
      tgtacttga atttaattt aattaactg tttgaattt 
      aactgtttt cgggtctaa aaatattaa cttctgtaa 
      aatatttta ttttagggg aaatctttg tagtatttg 
      tttgcaact ttcaacaaa aatagtcgg aaaaaatgt 
      aattaattg ttcagagga aaaattgcc tcccgataa 
      agttttggc aacattgcc ccaaaatac aaaaagata 
      atttcttaa tattccaca ttttctatt gtgcgttca 
      atgaaacag tgaaataat gttcttaag atgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY