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Gene information
General information
TC011092 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 536737..539680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011092
    iBeetle screen iB_06974

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011092 RA
      MYLVFLLSP FITASSPTS DLRERIRSG DFRKACSTT 
      VNLVQPPNR VNTTLKLAN LRNLMRNTS FTRDQRLLD 
      AYIVPSQDE HQNEFVEDH DKRLQFISG FSGSYGYAV 
      ITETKAVLW TDGRYHLQA DNETDCNWK LMRQHIYYV 
      PNISQWLRE TRPQGGVMG ADPQLFSQS KWEELSVAL 
      RNVKWELIE IQTDLIDVI WTNRPARRN KNAFVLEEK 
      YSGRKWTKK IHNVRKTVQ KLQADALVV TSLDEIGWL 
      LNIRGRDIP SSPLVRSYL LLDMERAWL YVNRSQLEA 
      NHVARYLTN SAKEANQLI EFFDYEEIC TGLASRAQL 
      YTRILLPPE STSRRIAQC VPPRKRLFV QSPIILFKA 
      RKNPIEIKG MHHAHVRDA ASMCEFFAY LDKMVREGL 
      TFTELDIVK VIDEFRFEQ LNSLGNSFP TIAAYGANG 
      AMPHYVPLV STNVMVGND STLVLDSGG QYLDGTTDV 
      TRTIHFGTP TKEQKEAYT RVLIGQIQL SMLTFPAFL 
      KTSAIDVMA RAPLWEIGL DYDHGTGHG VGSFLNVHE 
      APISLYFNN PSSIFPEND ILKPGYFLS NEPGYYKEN 
      DFGIRLENV MEVIEKKWL RTIHGTNYL GFRTVTLVP 
      YEPKLIDLS LLSKHQIQW LNQYNDRIR IHVGAELKR 
      QNFTKGLFW MMDQTRHFP ENGGKNHGI DLTMVALAA 
      ILIYCL
    CDS sequence
    • >TC011092 RA
      atgtactta gtttttcta ttatctccc tttatcact 
      gcctcctcc cccacctcc gacctgcgc gagcgaatc 
      agaagcggc gacttccga aaggcgtgt tcaacaact 
      gtcaactta gtccagccc ccaaaccgg gtcaacacc 
      accctaaaa cttgcaaat ttgcgaaat ttgatgcga 
      aacaccagc tttacacgc gaccaacgc ctactagac 
      gcctatatt gtgccatca caggacgaa catcaaaac 
      gaatttgtg gaagaccac gacaaacgc ctccagttc 
      atttcgggt ttttcgggt agttatggt tacgcggtt 
      ataaccgaa acgaaggcg gtgctatgg accgatggg 
      cggtaccat ttacaagcc gataacgag actgattgt 
      aattggaag ttgatgcgt caacacatt tactacgtt 
      ccgaatatc agccaatgg ttacgcgag acgcgccca 
      cagggcggg gtcatgggg gccgacccg caacttttc 
      agccaatcg aaatgggag gagctttcc gttgcgctt 
      agaaatgtc aagtgggag ttgattgaa atacagact 
      gatttgatt gacgtgatt tggaccaat cggccggca 
      cgacgcaat aaaaacgcc tttgttttg gaggagaaa 
      tattcaggt cggaaatgg accaagaaa attcacaac 
      gtgcgtaaa accgtccag aaactacaa gccgacgct 
      ttggttgtg acgtcactg gatgaaatt ggttggttg 
      ttgaacata agggggcgt gacattccg tccagccca 
      cttgtgcgt agttatttg ttattggat atggagagg 
      gcgtggctt tatgtcaat cggagccaa ttggaggcc 
      aatcacgtc gctcggtac ttgacgaat tcggcgaaa 
      gaagccaat caactgatc gaattcttt gattacgag 
      gagatttgt acgggattg gcttcacgt gcgcaactt 
      tatacccga attctcctc ccacctgag tcaacaagt 
      aggcggatc gctcagtgt gtcccgccc cggaaacgc 
      ctctttgtc caatcgcca atcattttg ttcaaagcg 
      cgcaagaac ccaatcgaa atcaaagga atgcaccac 
      gcccatgtg cgagacgcc gcctccatg tgcgagttt 
      ttcgcctac ttggacaaa atggttagg gaaggtttg 
      acgtttaca gagttggat attgtcaaa gtgatcgat 
      gagttccgg tttgaacaa ttgaacagt cttgggaat 
      agtttccca accatagcc gcctacggg gccaatggt 
      gccatgccc cattatgtc ccattggtt agtaccaac 
      gtcatggtg gggaacgac agtaccttg gtacttgac 
      tcgggtggg cagtacctt gatggtacc accgatgtc 
      acccgtacc atacacttt ggcacgccc actaaagag 
      caaaaagag gcgtacacg agggttctg attggccag 
      attcaactg tcaatgttg acttttccc gcctttttg 
      aaaacgtca gccattgac gtgatggcg agggcgcca 
      ctgtgggaa atagggctg gactatgac catgggacg 
      ggacatggg gttggttca ttcctcaat gtccatgaa 
      gcaccaatt tcgctttat ttcaataac ccgtcgtca 
      atattcccc gaaaatgac attctcaaa cctgggtac 
      tttctctcc aatgaacct gggtattac aaagaaaat 
      gattttggc attcgattg gaaaacgtg atggaagtg 
      attgagaag aaatggtta cggaccata catggtacc 
      aactatctc gggttcaga actgtcact ttggtacca 
      tacgaaccc aaacttatc gatttgagt ctactttca 
      aagcatcag atccaatgg ttgaaccaa tacaatgac 
      aggatacgt atacacgtg ggggcggag cttaaaaga 
      cagaatttt accaaaggg cttttctgg atgatggac 
      caaacgagg cattttcca gaaaatggc ggcaagaac 
      catggaatt gacctaacc atggtggca ctagccgcc 
      attttaata tattgtctg tga
    mRNA sequence
    • >TC011092 RA
      agcaaatta ttgaaattt tcacacgtc aaccctctg 
      atgttttat tatctcata attagatga agattcaag 
      attcaagat tcaagattt tattaataa ttagtgtga 
      ttcttaaaa aatgtactt agtttttct attatctcc 
      ctttatcac tgcctcctc ccccacctc cgacctgcg 
      cgagcgaat cagaagcgg cgacttccg aaaggcgtg 
      ttcaacaac tgtcaactt agtccagcc cccaaaccg 
      ggtcaacac caccctaaa acttgcaaa tttgcgaaa 
      tttgatgcg aaacaccag ctttacacg cgaccaacg 
      cctactaga cgcctatat tgtgccatc acaggacga 
      acatcaaaa cgaatttgt ggaagacca cgacaaacg 
      cctccagtt catttcggg tttttcggg tagttatgg 
      ttacgcggt tataaccga aacgaaggc ggtgctatg 
      gaccgatgg gcggtacca tttacaagc cgataacga 
      gactgattg taattggaa gttgatgcg tcaacacat 
      ttactacgt tccgaatat cagccaatg gttacgcga 
      gacgcgccc acagggcgg ggtcatggg ggccgaccc 
      gcaactttt cagccaatc gaaatggga ggagctttc 
      cgttgcgct tagaaatgt caagtggga gttgattga 
      aatacagac tgatttgat tgacgtgat ttggaccaa 
      tcggccggc acgacgcaa taaaaacgc ctttgtttt 
      ggaggagaa atattcagg tcggaaatg gaccaagaa 
      aattcacaa cgtgcgtaa aaccgtcca gaaactaca 
      agccgacgc tttggttgt gacgtcact ggatgaaat 
      tggttggtt gttgaacat aagggggcg tgacattcc 
      gtccagccc acttgtgcg tagttattt gttattgga 
      tatggagag ggcgtggct ttatgtcaa tcggagcca 
      attggaggc caatcacgt cgctcggta cttgacgaa 
      ttcggcgaa agaagccaa tcaactgat cgaattctt 
      tgattacga ggagatttg tacgggatt ggcttcacg 
      tgcgcaact ttatacccg aattctcct cccacctga 
      gtcaacaag taggcggat cgctcagtg tgtcccgcc 
      ccggaaacg cctctttgt ccaatcgcc aatcatttt 
      gttcaaagc gcgcaagaa cccaatcga aatcaaagg 
      aatgcacca cgcccatgt gcgagacgc cgcctccat 
      gtgcgagtt tttcgccta cttggacaa aatggttag 
      ggaaggttt gacgtttac agagttgga tattgtcaa 
      agtgatcga tgagttccg gtttgaaca attgaacag 
      tcttgggaa tagtttccc aaccatagc cgcctacgg 
      ggccaatgg tgccatgcc ccattatgt cccattggt 
      tagtaccaa cgtcatggt ggggaacga cagtacctt 
      ggtacttga ctcgggtgg gcagtacct tgatggtac 
      caccgatgt cacccgtac catacactt tggcacgcc 
      cactaaaga gcaaaaaga ggcgtacac gagggttct 
      gattggcca gattcaact gtcaatgtt gacttttcc 
      cgccttttt gaaaacgtc agccattga cgtgatggc 
      gagggcgcc actgtggga aatagggct ggactatga 
      ccatgggac gggacatgg ggttggttc attcctcaa 
      tgtccatga agcaccaat ttcgcttta tttcaataa 
      cccgtcgtc aatattccc cgaaaatga cattctcaa 
      acctgggta ctttctctc caatgaacc tgggtatta 
      caaagaaaa tgattttgg cattcgatt ggaaaacgt 
      gatggaagt gattgagaa gaaatggtt acggaccat 
      acatggtac caactatct cgggttcag aactgtcac 
      tttggtacc atacgaacc caaacttat cgatttgag 
      tctactttc aaagcatca gatccaatg gttgaacca 
      atacaatga caggatacg tatacacgt gggggcgga 
      gcttaaaag acagaattt taccaaagg gcttttctg 
      gatgatgga ccaaacgag gcattttcc agaaaatgg 
      cggcaagaa ccatggaat tgacctaac catggtggc 
      actagccgc cattttaat atattgtct gtgacaata 
      ataaaatgg acgcccgtt ttcgcacat aattttttg 
      tattttaaa tgttttatt aatttataa aatcacgtt 
      tactaagca caataaaag tcagttatt tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY