Status
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Gene information
General information
TC032437
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003616
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9101901..9104269
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032437
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06970

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva random segmentation/division not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032437 RA
      MVKKLPPKT IAEFRRSAE TENFQIPEK PENRKCTDL 
      GYLIFFLLV VLITTIFVA CSSLGTNYN RFLNGYDKC 
      GDTCGTSNL AVKTVQCSG KDLTNKPFM TFKYANRTN 
      DSKSKRDIL PDICDNKCD PPKRLVFHR CIPDLNKTS 
      YRKTTIAFA EFRNDLKLA INTIIIPMA IIGLVVSLI 
      FLHGLRYSP KITITATIT SALICLFCF TSYIWYWFA 
      RNLDEKKLI YIGIVSSCA IFLQSIFIC YFHDRYSLI 
      IKMFREATQ VIFAMPSLI FVPLGFVAI LAVVTASIV 
      FLGALISTN QYLSEIPDH PSVYEYRDT RLAEGALAC 
      IVVFGIWTF ALANGCQCM IVSGAISTY YFTRDKSTL 
      QKPVRASFY VLMRYHLGT VALGSLCIT FFSIARLCT 
      EFLAKRCKN KWSLRGLSR VLSWIEGAA EYLSKRAYI 
      QTAMHGEPL FRSGLRGAR LLWANFLDN IVLYGVGNF 
      IVVCITLSA VFLGTIFAI IVYKTAGLK VSYFWTIVA 
      IGAGISGTI IYFFTQVLN VAVETIFIC FCEDKLLHS 
      ANNKDYFMS EELKTLIET AKKAARRQE AD
    CDS sequence
    • >TC032437 RA
      atggttaaa aaactcccc ccaaaaacc attgccgaa 
      tttcgaaga agtgcagaa actgaaaat tttcaaatt 
      cctgaaaag ccggaaaat cgcaaatgt accgatctc 
      ggctactta atatttttc ctccttgtt gttttaata 
      acgacaatt tttgtggct tgtagttct cttggaacg 
      aattataac cgatttttg aacggttat gataaatgt 
      ggcgatacc tgcggtacc tccaacctg gccgttaaa 
      accgttcaa tgttcagga aaagattta acaaacaaa 
      ccattcatg acgtttaaa tacgccaat cggacaaac 
      gactcaaaa agcaaaagg gatatttta ccagacatt 
      tgtgacaac aaatgcgac cctcctaag agactagta 
      tttcatcgg tgcatccct gacctgaat aaaacctcc 
      tacaggaaa actaccata gcttttgcc gaatttcgg 
      aacgacctc aaacttgcc ataaacacg attatcatc 
      ccgatggct atcatagga ttagtagtt tcgctgatt 
      tttctccac ggactgcgt tactcgcca aaaattaca 
      ataactgca acaatcacc tccgccctt atctgtctc 
      ttttgcttc acgtcatac atttggtac tggtttgcc 
      agaaatctg gacgagaaa aaacttatc tacattgga 
      attgtatcg tcatgtgcg atttttctc caatctatt 
      ttcatatgt tacttccac gatcgctac tctctaatt 
      atcaaaatg ttccgggaa gcgactcaa gtaattttt 
      gcgatgccc tctctcatt tttgttcct ttgggtttt 
      gtggctatc cttgctgtg gttactgct agtatcgtc 
      ttccttggt gccttaatc agcaccaat caatatttg 
      agcgaaatt ccagaccat ccatctgtt tacgaatac 
      agagataca agactggcc gaaggtgcc ctcgcatgt 
      attgttgtt tttggtatt tggacgttt gctttggcc 
      aatgggtgc caatgcatg atcgtttct ggtgccatt 
      tccacttat tatttcaca agggacaaa tcaacttta 
      cagaaacca gttcgtgcg agtttttac gtcctgatg 
      cgttaccac cttggtact gttgcttta ggttcgctt 
      tgtataaca tttttttcg attgctcga ctgtgtact 
      gagtttctc gcgaaacgt tgtaaaaac aaatggtct 
      ttgagaggt ttaagccgt gttttgtcg tggattgag 
      ggagcggcg gaatatttg tccaaaagg gcttatatc 
      caaactgca atgcatggg gagccactg tttcggagc 
      ggattacgg ggagcgaga cttctttgg gcaaatttt 
      ttggacaat attgtgctt tatggagtc gggaatttt 
      attgttgtt tgtatcacg ctttctgcg gtatttctg 
      ggaacaata ttcgcgatt attgtctat aaaacggca 
      ggtttaaaa gtgagctat ttctggacg atagttgca 
      atcggcgct ggcatttcc ggaactata atctatttt 
      ttcacacaa gttttaaat gtggccgtc gagaccata 
      tttatatgt ttctgtgaa gacaaactc ttacatagt 
      gcaaacaat aaggattat tttatgagt gaagagctg 
      aagacgctg atagaaacg gctaaaaag gccgcaagg 
      aggcaagaa gcagactaa
    mRNA sequence
    • >TC032437 RA
      tcgctaata tttgactgg gcaaaaatt atttcacca 
      aaatattga cttggggaa gtttacgtc acactgtca 
      agtcctaat taataacac tatggttaa aaaactccc 
      cccaaaaac cattgccga atttcgaag aagtgcaga 
      aactgaaaa ttttcaaat tcctgaaaa gccggaaaa 
      tcgcaaatg taccgatct cggctactt aatattttt 
      cctccttgt tgttttaat aacgacaat ttttgtggc 
      ttgtagttc tcttggaac gaattataa ccgattttt 
      gaacggtta tgataaatg tggcgatac ctgcggtac 
      ctccaacct ggccgttaa aaccgttca atgttcagg 
      aaaagattt aacaaacaa accattcat gacgtttaa 
      atacgccaa tcggacaaa cgactcaaa aagcaaaag 
      ggatatttt accagacat ttgtgacaa caaatgcga 
      ccctcctaa gagactagt atttcatcg gtgcatccc 
      tgacctgaa taaaacctc ctacaggaa aactaccat 
      agcttttgc cgaatttcg gaacgacct caaacttgc 
      cataaacac gattatcat cccgatggc tatcatagg 
      attagtagt ttcgctgat ttttctcca cggactgcg 
      ttactcgcc aaaaattac aataactgc aacaatcac 
      ctccgccct tatctgtct cttttgctt cacgtcata 
      catttggta ctggtttgc cagaaatct ggacgagaa 
      aaaacttat ctacattgg aattgtatc gtcatgtgc 
      gatttttct ccaatctat tttcatatg ttacttcca 
      cgatcgcta ctctctaat tatcaaaat gttccggga 
      agcgactca agtaatttt tgcgatgcc ctctctcat 
      ttttgttcc tttgggttt tgtggctat ccttgctgt 
      ggttactgc tagtatcgt cttccttgg tgccttaat 
      cagcaccaa tcaatattt gagcgaaat tccagacca 
      tccatctgt ttacgaata cagagatac aagactggc 
      cgaaggtgc cctcgcatg tattgttgt ttttggtat 
      ttggacgtt tgctttggc caatgggtg ccaatgcat 
      gatcgtttc tggtgccat ttccactta ttatttcac 
      aagggacaa atcaacttt acagaaacc agttcgtgc 
      gagttttta cgtcctgat gcgttacca ccttggtac 
      tgttgcttt aggttcgct ttgtataac atttttttc 
      gattgctcg actgtgtac tgagtttct cgcgaaacg 
      ttgtaaaaa caaatggtc tttgagagg tttaagccg 
      tgttttgtc gtggattga gggagcggc ggaatattt 
      gtccaaaag ggcttatat ccaaactgc aatgcatgg 
      ggagccact gtttcggag cggattacg gggagcgag 
      acttctttg ggcaaattt tttggacaa tattgtgct 
      ttatggagt cgggaattt tattgttgt ttgtatcac 
      gctttctgc ggtatttct gggaacaat attcgcgat 
      tattgtcta taaaacggc aggtttaaa agtgagcta 
      tttctggac gatagttgc aatcggcgc tggcatttc 
      cggaactat aatctattt tttcacaca agttttaaa 
      tgtggccgt cgagaccat atttatatg tttctgtga 
      agacaaact cttacatag tgcaaacaa taaggatta 
      ttttatgag tgaagagct gaagacgct gatagaaac 
      ggctaaaaa ggccgcaag gaggcaaga agcagacta 
      ataataaag aaaacttaa aata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY