Status
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Gene information
General information
TC032433
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003611,TC003612
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8996620..9059249
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032433
    iBeetle screen iB_06968

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032433 RA
      MERYLKDEP KMQSFKKLP SDMERTLYG VNCIEDYSD 
      RHLDAFSSA SSPCSWDEG ALSCAVLVK QEPLDDEED 
      EDDAFEGRV HADAGDALQ LRLVARNPA TLTPPSSPE 
      SGPGHSNGS SSASELDVC GLRVGSVSR NAIVRVRGL 
      ARYISVVPR TTQSPVSSP ASTTTVKHR LDHSPDSKR 
      RIHKCQFLG CKKVYTKSS HLKAHQRTH TGNAISRWW 
      SGFGPDPSP PAIPAAIFH ITPPANLFG HCRGGGLIV 
      RHDRSGKAR LQSRSSEKP YKCSWEGCE WRFARSDEL 
      TRHYRKHTG AKPFKCRHC DRCFSRSDH LALHMKRHA
    CDS sequence
    • >TC032433 RA
      atggagcgc tacctgaag gacgagccg aagatgcag 
      tcgtttaaa aagttgcca tcggacatg gaaaggacg 
      ttatacgga gtgaattgt atagaggac tatagtgac 
      cgtcacttg gacgcgttc agttcggcg agttcgccg 
      tgctcctgg gacgagggg gcgctgtcg tgcgccgtg 
      ctcgtgaag caggagccc ctcgacgac gaggaggac 
      gaggacgac gccttcgaa gggcgagtg cacgctgac 
      gccggcgat gcgctgcag ctgcggctg gtggcgagg 
      aatccggcg acgttgacg ccgccatcg agtcccgaa 
      tccggcccg ggccacagt aacggaagt tcgagtgcg 
      agcgaattg gacgtttgc ggactacgg gtgggcagt 
      gtgtcgcgg aatgccatt gtgcgggtt agaggactg 
      gcaaggtac atatcggtc gtccccaga accacacag 
      agtcccgtt tcttcaccc gcttccacg acgacggtc 
      aagcatcga ctcgaccat tcgcctgac tccaaacga 
      aggatacac aagtgccaa tttctcggc tgcaagaag 
      gtctacacc aagagctca catctcaag gcgcaccaa 
      agaacacac acaggtaat gcaatttct cgttggtgg 
      tcgggcttt ggccccgac cccagtccg ccagcgatt 
      cccgccgct attttccac ataactccg ccggcgaat 
      ctattcggc cactgccgg ggcggaggg ctaattgtc 
      cgtcatgac cgatcgggc aaagcccgt ctccaaagc 
      agatcaagc gagaagccg tacaagtgc tcgtgggaa 
      ggctgtgag tggcggttc gcccgttcg gacgaactc 
      acccgccac taccgcaaa cacacgggc gccaaaccg 
      ttcaagtgc cgccactgc gaccgatgc tttagccgt 
      tcggaccac ttggccctg cacatgaag cgccacgct 
      tag
    mRNA sequence
    • >TC032433 RA
      tttgtatcg atttgcctt caagttgct aattgttgc 
      agacgtgct acgagatgg agcgctacc tgaaggacg 
      agccgaaga tgcagtcgt ttaaaaagt tgccatcgg 
      acatggaaa ggacgttat acggagtga attgtatag 
      aggactata gtgaccgtc acttggacg cgttcagtt 
      cggcgagtt cgccgtgct cctgggacg agggggcgc 
      tgtcgtgcg ccgtgctcg tgaagcagg agcccctcg 
      acgacgagg aggacgagg acgacgcct tcgaagggc 
      gagtgcacg ctgacgccg gcgatgcgc tgcagctgc 
      ggctggtgg cgaggaatc cggcgacgt tgacgccgc 
      catcgagtc ccgaatccg gcccgggcc acagtaacg 
      gaagttcga gtgcgagcg aattggacg tttgcggac 
      tacgggtgg gcagtgtgt cgcggaatg ccattgtgc 
      gggttagag gactggcaa ggtacatat cggtcgtcc 
      ccagaacca cacagagtc ccgtttctt cacccgctt 
      ccacgacga cggtcaagc atcgactcg accattcgc 
      ctgactcca aacgaagga tacacaagt gccaatttc 
      tcggctgca agaaggtct acaccaaga gctcacatc 
      tcaaggcgc accaaagaa cacacacag gtaatgcaa 
      tttctcgtt ggtggtcgg gctttggcc ccgacccca 
      gtccgccag cgattcccg ccgctattt tccacataa 
      ctccgccgg cgaatctat tcggccact gccggggcg 
      gagggctaa ttgtccgtc atgaccgat cgggcaaag 
      cccgtctcc aaagcagat caagcgaga agccgtaca 
      agtgctcgt gggaaggct gtgagtggc ggttcgccc 
      gttcggacg aactcaccc gccactacc gcaaacaca 
      cgggcgcca aaccgttca agtgccgcc actgcgacc 
      gatgcttta gccgttcgg accacttgg ccctgcaca 
      tgaagcgcc acgcttaga cggcctacc tctccgccg 
      catcactgc cagttaccg ctagtgtac atacaatac 
      tttagatag gtgtggatg gactcttgg agcagcaca 
      cacgtagcg agggtttgc ttcctcagt gatgttcat 
      tcctgtgat cgacatcgt cggctaggg ccgaacaca 
      cctacagaa cgcgcgcgg cccgcgggc cgcactgta 
      gctgtacgg cgcggcggc ggcgccccc cacgtccga 
      gggacgcta aattctcca gatcaaaat tttttatcg 
      gttcggtgg ctgttggcg ggggcggcg ccgccgcga 
      ggatagtca tgttagatt taggtgatg gagtctttg 
      gacggcatt tttcgggaa tgttgtgtg agggctctg 
      gactttgtt agtctgtac gaatgtgtc aaagtatta 
      actgaagac tgtgcaggc gcacaccgc cggatatta 
      ggctaaatg tcactattt gtaataaaa aaagaatca 
      aaaatgtaa cgtatagta gcgcgaaca agtttttga 
      aaaaactga taatgaaaa gtattataa taggtaatt 
      atatttttt gagaaagtg accttggat gaaaatcta 
      cttttttca aaaatgcaa cgataagcc gtgtacagt 
      tgtgtacaa tgtgcattt gagcggcgt cgcgacgtc 
      tgttggacg ccgcaccct ccgacgcat tccttctcg 
      atgcattct atatttcta cttccctga ctaggcata 
      gcgtgactg ttgtgtgat cagagaccg tgtatatag 
      atttttagt gttatagca ttttgtaca gctgcatgc 
      tttgttaat tagttcggt acattttac taatatgtg 
      tgcgagcaa aacgctgtc caacgccga aatactctt 
      cagacgatt ttttgccaa atgcaaagc taaaacacg 
      ggaaaaaat cgcacttga aagattaaa agactctga 
      ctagttcag ctcaacaac gcaaatgaa ccaattaaa 
      ttgcttcaa ttttggtca cgtgatcag ttaatcacg 
      catttaatt gcggattaa attaatgac gcttctata 
      aaccgcacc taaatatat atgacctgc acttgcctt 
      aatgatttt cattaagat acaggcatt tgaaaaaaa 
      tatattttt attgcaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY