Status
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Gene information
General information
TC032421
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003586
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8140964..8144858
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032421
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06958

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032421 RA
        MEDSDLTEH LKINYSGIK KNGKIYLLI LLATIPGLC 
        FSSVTLHAI KIGASETLR CKINSFENA SWKFQELSR 
        PDGVHKECF IFNYDYDKL AHMTLKDAK KLVDKDYAN 
        RTRIICTKF VADENNSRP AAYPQEFAN YCESLRYDF 
        LYDVGAVLP YILGCLLVV PLADIYGRK RILSLGCHL 
        AYISVFIKI IMLHFTREA IGEAVINYA YPVLKYTTE 
        AIIIEITGN ELRPGAFVL TFLGLQVPQ FLIGVMPPL 
        MYWKYATLL AYSTVLLLP LLLWFVPEP SLWFISGQQ 
        TRTALFASE TLNDSPRHN DTLLLLRNK VITFKKANK 
        LVQEFRHKI NWKEFISIN VISISVGFL SFESYSKHK 
        LMYASLENG WMRILHFSS GLFGVIFTI PCVYIMNGV 
        KFSLAVLFG GCGALFACL CFLNSDSFD NWFAIVTFF 
        IEILSVIVK ILFEFYLQR IFPTSHRCF CTGICGATM 
        TFGLLIVIG IQQISSLAR EIITSVASI SSCIISIFF 
        VKNLSRMDS LHINFAA
      CDS sequence
      • >TC032421 RA
        atggaagat tccgattta actgaacat ttgaagatc 
        aattacagt ggcataaaa aagaatggc aaaatttat 
        cttttgata ttattggca acaatccct ggcttgtgc 
        ttctcttcg gtaacatta cacgccata aaaattgga 
        gcaagcgaa actttgcga tgcaaaatt aatagtttc 
        gaaaacgcg tcttggaaa tttcaagag ttatcacgc 
        ccggatggc gtccacaaa gaatgtttt atttttaat 
        tatgactat gataaactt gctcacatg acactcaaa 
        gacgccaaa aaacttgtt gacaaagac tatgcaaat 
        aggacacga atcatttgt acaaaattt gtggccgac 
        gaaaataat tcgcgtcct gcggcgtat cctcaggag 
        ttcgccaat tattgtgaa agtctcagg tacgatttt 
        ttgtacgat gttggggcc gtacttccg tatatcttg 
        ggctgttta ttggtggtg ccgctagcc gacatatac 
        ggacgaaaa agaatttta tcgctgggt tgtcatctt 
        gcgtacata tcggttttt ataaaaatt attatgttg 
        cattttact agagaagca atcggcgaa gctgtaatt 
        aattacgct tatccagtg ttaaaatac acaactgaa 
        gctatcata attgaaata acgggaaac gagctcaga 
        cctggcgcg ttcgtatta acttttttg ggactccaa 
        gttccccaa tttttaatt ggagttatg ccaccactt 
        atgtactgg aaatatgca acacttttg gcttacagt 
        acagttttg ttgttacca ctccttctg tggtttgtg 
        cccgaacca tcactctgg ttcatttca ggacagcaa 
        acaagaacg gcattgttt gcctcagaa acgttgaat 
        gattctcca agacacaat gacacgctc ctgctccta 
        cgcaataaa gtaatcact ttcaaaaaa gcgaataaa 
        ctggtacaa gagttcagg cacaaaatt aattggaag 
        gagtttatc tcaattaac gtaatttca atcagcgtc 
        gggttttta tcatttgaa tcatattcg aaacataaa 
        ctaatgtat gctagtctg gaaaatggc tggatgaga 
        attttacat ttctctagt ggactattt ggcgttatt 
        ttcacaatt ccgtgcgtc tacataatg aacggtgtt 
        aaattttcg ctggcggtt ttgtttggc ggatgcgga 
        gctctgttt gcctgtttg tgtttttta aattcagat 
        tcttttgat aattggttc gcaatcgtg acatttttt 
        atcgaaatt ttatcagtt attgtgaaa attttgttc 
        gagttttac cttcaaaga atttttccg accagtcac 
        agatgcttt tgtactgga atttgtggt gcaactatg 
        acctttgga ttacttatt gtaattggc atccagcaa 
        ataagcagt ttggcgaga gaaattata acatcggtt 
        gcttcaata tcatcttgc ataatctct atttttttc 
        gtaaaaaat ttgtcgaga atggattcg ctgcacata 
        aattttgca gcgtaa
      mRNA sequence
      • >TC032421 RA
        ttttagata acttccatc acccaaaaa ttataaaag 
        tgagaaatg caagattct agatgccga aaaaacaac 
        caaaaccaa atttttaaa tggaagatt ccgatttaa 
        ctgaacatt tgaagatca attacagtg gcataaaaa 
        agaatggca aaatttatc ttttgatat tattggcaa 
        caatccctg gcttgtgct tctcttcgg taacattac 
        acgccataa aaattggag caagcgaaa ctttgcgat 
        gcaaaatta atagtttcg aaaacgcgt cttggaaat 
        ttcaagagt tatcacgcc cggatggcg tccacaaag 
        aatgtttta tttttaatt atgactatg ataaacttg 
        ctcacatga cactcaaag acgccaaaa aacttgttg 
        acaaagact atgcaaata ggacacgaa tcatttgta 
        caaaatttg tggccgacg aaaataatt cgcgtcctg 
        cggcgtatc ctcaggagt tcgccaatt attgtgaaa 
        gtctcaggt acgattttt tgtacgatg ttggggccg 
        tacttccgt atatcttgg gctgtttat tggtggtgc 
        cgctagccg acatatacg gacgaaaaa gaattttat 
        cgctgggtt gtcatcttg cgtacatat cggttttta 
        taaaaatta ttatgttgc attttacta gagaagcaa 
        tcggcgaag ctgtaatta attacgctt atccagtgt 
        taaaataca caactgaag ctatcataa ttgaaataa 
        cgggaaacg agctcagac ctggcgcgt tcgtattaa 
        cttttttgg gactccaag ttccccaat ttttaattg 
        gagttatgc caccactta tgtactgga aatatgcaa 
        cacttttgg cttacagta cagttttgt tgttaccac 
        tccttctgt ggtttgtgc ccgaaccat cactctggt 
        tcatttcag gacagcaaa caagaacgg cattgtttg 
        cctcagaaa cgttgaatg attctccaa gacacaatg 
        acacgctcc tgctcctac gcaataaag taatcactt 
        tcaaaaaag cgaataaac tggtacaag agttcaggc 
        acaaaatta attggaagg agtttatct caattaacg 
        taatttcaa tcagcgtcg ggtttttat catttgaat 
        catattcga aacataaac taatgtatg ctagtctgg 
        aaaatggct ggatgagaa ttttacatt tctctagtg 
        gactatttg gcgttattt tcacaattc cgtgcgtct 
        acataatga acggtgtta aattttcgc tggcggttt 
        tgtttggcg gatgcggag ctctgtttg cctgtttgt 
        gttttttaa attcagatt cttttgata attggttcg 
        caatcgtga catttttta tcgaaattt tatcagtta 
        ttgtgaaaa ttttgttcg agttttacc ttcaaagaa 
        tttttccga ccagtcaca gatgctttt gtactggaa 
        tttgtggtg caactatga cctttggat tacttattg 
        taattggca tccagcaaa taagcagtt tggcgagag 
        aaattataa catcggttg cttcaatat catcttgca 
        taatctcta tttttttcg taaaaaatt tgtcgagaa 
        tggattcgc tgcacataa attttgcag cgtaatatt 
        tggataaat aaaagaccg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY