Status
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Gene information
General information
TC032414
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002886,TC002885
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7265577..7269363
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032414
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06953

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva potentially partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032414 RA
        MAYCSKGRV CITLKKTSQ LKTDVDVTI KEDFDFAEA 
        LESVYPLLE IVFSYLDYS DLQKASMVK KSWQVTAGR 
        ILEKRNKIS WITVFRKKR QSYVHYSNN YLYNNVNLG 
        IILFNYRAV SLNDFFCCH ENLSSRKIR FSDFLSDQV 
        VPSGTEYCA IGCPGVASM FSKQVVNKS SVPLFDGCF 
        IPPIPNVRV SMFYCNPVC RNDDHFLAD EKVKCILFF 
        SQVDFAQEF YDMLDHLIS EEECDKVAI AGGIVRRTT 
        VFPQLPEYH TLRRLNSLC IAFAEEGFC DNSVFDASS 
        VVINGNNLT SEDFEDHLL KFKRRIVLR SNSLAFRIC 
        CSAKMRKNH ESRIFNKVF PDTPLIGLE AEGEIGWNC 
        FNHSDNNEE VENKAKKCK EGYPIVQHQ WSTVIALVT 
        WGHLIHNTS RDL
      CDS sequence
      • >TC032414 RA
        atggcttac tgtagtaaa ggcagagta tgtatcact 
        ctgaagaag acaagccag cttaaaacc gacgtggat 
        gtaacaatt aaggaagat tttgacttt gccgaagcc 
        ctagaatca gtctacccc ttactcgaa attgtgttc 
        agttattta gactacagt gacttacaa aaagcctcc 
        atggtgaaa aaatcgtgg caggtaact gctggtcgg 
        attctagaa aagcgcaat aaaatcagc tggataact 
        gtctttcga aaaaagcgg caaagttat gtccactac 
        agcaacaat tacttgtac aacaatgtc aatttgggg 
        attatatta ttcaattat cgcgcagtt tctctgaac 
        gactttttc tgctgccac gaaaacttg tcttcacgg 
        aaaatcaga ttttcagat tttttatct gatcaggtg 
        gtccccagt gggacagaa tattgtgca ataggatgc 
        cctggagtg gcgtcgatg ttttccaaa caagttgta 
        aataaatcc agcgtacct ctctttgat ggctgtttt 
        ataccgcct ataccaaat gtcagggtt tcaatgttt 
        tactgtaat cctgtttgt cgaaacgat gatcatttt 
        ctcgcagat gagaaggtc aaatgcata ctgtttttt 
        tctcaagtc gactttgct caggaattt tacgatatg 
        ttagatcac ctaatttca gaagaggaa tgcgacaag 
        gttgctata gctgggggc atagtccgc aggacaacg 
        gtgttccca caactgcca gaatatcac acccttcgc 
        agactaaac agcctctgc atagctttc gcagaggag 
        ggtttttgt gataatagt gtgtttgac gcgtcttcc 
        gtcgtaata aacggaaac aacttaact tcagaggac 
        tttgaagac catcttctc aagttcaaa cgcagaata 
        gtcttaagg agtaacagt ctggcgttt agaatatgt 
        tgctcggcc aaaatgcgg aaaaaccac gagtcccga 
        attttcaat aaagttttc cccgacact cctctaatc 
        ggtctagaa gctgagggg gaaattggg tggaattgc 
        ttcaatcac agcgacaat aacgaggaa gttgaaaat 
        aaagcgaaa aagtgcaag gaaggatac cccatagtg 
        caacaccag tggtcaact gtgatagca cttgttacc 
        tggggacac ttgatacac aacacgagt agagattta 
        taa
      mRNA sequence
      • >TC032414 RA
        caaaatgac agcgtgatt tattttgat ggttgaaaa 
        aattttcaa taacaataa tattgaaga aacattaat 
        tatacccca aaataaatg acgtaggct ttaaggata 
        atcagtgtt atacacatc agcagctat ggcttactg 
        tagtaaagg cagagtatg tatcactct gaagaagac 
        aagccagct taaaaccga cgtggatgt aacaattaa 
        ggaagattt tgactttgc cgaagccct agaatcagt 
        ctacccctt actcgaaat tgtgttcag ttatttaga 
        ctacagtga cttacaaaa agcctccat ggtgaaaaa 
        atcgtggca ggtaactgc tggtcggat tctagaaaa 
        gcgcaataa aatcagctg gataactgt ctttcgaaa 
        aaagcggca aagttatgt ccactacag caacaatta 
        cttgtacaa caatgtcaa tttggggat tatattatt 
        caattatcg cgcagtttc tctgaacga ctttttctg 
        ctgccacga aaacttgtc ttcacggaa aatcagatt 
        ttcagattt tttatctga tcaggtggt ccccagtgg 
        gacagaata ttgtgcaat aggatgccc tggagtggc 
        gtcgatgtt ttccaaaca agttgtaaa taaatccag 
        cgtacctct ctttgatgg ctgttttat accgcctat 
        accaaatgt cagggtttc aatgtttta ctgtaatcc 
        tgtttgtcg aaacgatga tcattttct cgcagatga 
        gaaggtcaa atgcatact gtttttttc tcaagtcga 
        ctttgctca ggaatttta cgatatgtt agatcacct 
        aatttcaga agaggaatg cgacaaggt tgctatagc 
        tgggggcat agtccgcag gacaacggt gttcccaca 
        actgccaga atatcacac ccttcgcag actaaacag 
        cctctgcat agctttcgc agaggaggg tttttgtga 
        taatagtgt gtttgacgc gtcttccgt cgtaataaa 
        cggaaacaa cttaacttc agaggactt tgaagacca 
        tcttctcaa gttcaaacg cagaatagt cttaaggag 
        taacagtct ggcgtttag aatatgttg ctcggccaa 
        aatgcggaa aaaccacga gtcccgaat tttcaataa 
        agttttccc cgacactcc tctaatcgg tctagaagc 
        tgaggggga aattgggtg gaattgctt caatcacag 
        cgacaataa cgaggaagt tgaaaataa agcgaaaaa 
        gtgcaagga aggataccc catagtgca acaccagtg 
        gtcaactgt gatagcact tgttacctg gggacactt 
        gatacacaa cacgagtag agatttata agtgactaa 
        caaacctcg aacaaatta aaatttgaa atttaatca 
        taaggtatt aactacgat tttcataaa cgggacata 
        ttattaagc tagcgacta ttttacttc cacaaaagt 
        gttttaaaa gttatttta catcgtgtg ataatctgt 
        aatttttat ttcaggtgc gaaaataaa gtttgggaa 
        aaaag
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY