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Gene information
General information
TC011099 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 411406..455013
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011099
    iBeetle screen iB_06952

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    yolk late deposition irregular - Number of animals showing the phenotype: 2
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4

    previtellogenic egg chamber late deposition irregular - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011099 RA
      MFKILISCL LFVHRLLCS CRYFHAKWC SYVMGCFLT 
      VGVLFSTLC LFAAGQEYI ITGPADTQF IPNYRQTPD 
      FNPFRPNFQ HVDSYLPQR DLQTSHLPY HLQQTLPQY 
      RATTKTPPR YSYQPQFDY RSSQPAPFR TQEQIVPST 
      HRPNYSNLF PHSHRNPQT ETFLGEITL QSPEDFKQE 
      IDTTEKSNV VTIGGRGNF LNHRTRIFA PDTVKKVPL 
      VREYYVNKN GNVFSTTRA PITQKTTTE SLRRKTTSG 
      TTSTTIEST TRRSRNGLD FESLLEEPI KNSNTNDDV 
      VEVVKSKSV EMTTLNSHN ESTTDFDYD TTLDPSVDY 
      DKDEGEEEE EEDPIKHLE ELRKQHPEE EDYVSEEEE 
      TPNSEETNN VRDEDYEEE EGTTTSTVR TTSSTTPST 
      IKTTIATPS TIRTTDATP STTTEEVPT TRKTEALLA 
      EPETVISVV TTKSVVNNT ILPTATPLP NSTITSENS 
      TDTWVVVAS VQTSRSVSG ARYLPSDDV KQDERIKLL 
      NEESTESTE TTDEPMTVL PTTKHKTST ESLIDKLDR 
      VQSDLSSSV FNGGLNNIA VITEMATTT PPPPKPVVQ 
      IRKFSPFAR PSTTPRTRK IEIVKQDDL TGLLPPGFK 
      PKYQNRKTT VKPDLELPQ GDEPPKIGR SSGISNKTK 
      ITVQDVSPF LPPGYKSTK DQDDSTKLL AEILGKVKP 
      KIVKVDDVS AFLPPGYKP TSGTTSTST TTTKSVDGI 
      LSKAKPVDD ISALLPPGY KSSTSTTKP KPSIEGVLA 
      HAKPVDDIS ALLPPGYKP SSTAKPVKP KGVDGLLAQ 
      AKPVDISAF LPPGYKART TQKPLTTPE KVPSSLLPP 
      GYKLRETTT TSSTTSTTT TTTSKPSGF KIVFPSRPG 
      GTHRKSPNR ITTPPSTMT EEPRATAPT IHKGWPSRA 
      TTEFTGWPT PSTTPISIE KLLEAARTA ATSTTPAVD 
      STTSTTTST TTTTTTTTP KPTTPGVCT GECDLAGTI 
      KLVGGAKWV PELLDRNTK EWQILANEV QTQLEDVFG 
      RSNVLNKWY RKIRIDGFS EGSVLVDYL VELNDLGRK 
      IDTQEIKKL FHDSLDDGA TNREGKSLN ETSKMALGK 
      FTVDPKFTD FVVLPKQVY PTVGYAEDN VLLPQWAIA 
      VIVIGLASL LFVIIFGVT VLVNRQKNA KKKAPVSLT 
      EDMLNELNK NHMGGFDNY GADDLYNME DVWNDRGFE 
      PKLAKKRSS GSLHDNSMS NLYDSWRSE WNGYYYNAY 
      YGNNNPGSS SSHYGRRRS DYDTNF
    CDS sequence
    • >TC011099 RA
      atgtttaaa atcctaatt tcttgcctc ttgttcgtc 
      catcggttg ttgtgttct tgtcgttac tttcacgcg 
      aagtggtgc agttatgtg atggggtgc tttctgaca 
      gtgggggtg ctcttctca accctttgt ctatttgct 
      gcaggtcag gagtacatc atcacgggc cctgcggac 
      acccaattc atccccaat tatcgccag acccccgac 
      ttcaacccc ttcagaccc aacttccaa cacgtggac 
      tcctacctc cctcaaagg gacctccaa acgtcccac 
      ctcccctac cacttgcaa caaaccctc ccgcagtac 
      cgtgccacc acgaaaaca cccccaagg tactcctac 
      caaccccag ttcgactat cgaagctca caaccagcg 
      ccattccgc acccaggag caaatagta ccatcaacc 
      catcggcca aactactcc aacttgttc ccacactcc 
      catcgtaac ccacaaact gaaaccttc cttggggaa 
      atcacactt caaagtcca gaagacttc aaacaggag 
      atagacacg actgagaaa agcaacgtt gtgacgatt 
      ggaggccga gggaatttt ttgaaccac aggacacga 
      atttttgct cctgatacc gtcaaaaaa gtaccactt 
      gtgagggag tactatgtt aacaaaaat gggaatgtt 
      tttagtacc acacgagct cccataacc caaaaaacg 
      acaactgaa agtttgaga cgaaaaact acaagtggt 
      actactagt actactatt gaaagtact actaggagg 
      agcaggaac gggttagat tttgagtca cttttggag 
      gaaccgatc aaaaattcc aacaccaat gatgatgtg 
      gtcgaagtt gtcaagtca aaaagtgtc gaaatgaca 
      actttgaat agtcacaat gagagtacc accgatttt 
      gactatgat acaactctt gatccgtca gtagactac 
      gataaagac gaaggagaa gaagaagaa gaagaagat 
      ccaatcaaa catctggaa gagttaagg aagcaacat 
      cctgaagaa gaagattac gttagtgaa gaagaggaa 
      actccgaat tctgaagaa accaacaat gttcgtgac 
      gaagattat gaagaagaa gaaggtacc accactagt 
      accgttaga actacttct agtactact cctagtacc 
      attaaaaca actattgct actcctagt actattaga 
      actactgat gctactcct agtactacc actgaagaa 
      gtaccaacc actcgcaaa actgaggct cttttggcc 
      gaaccggag accgtaatt tcggtcgta acgactaaa 
      agtgtggtt aacaacacc atccttccg acagctaca 
      cctttacct aactctacc ataacttca gagaatagt 
      accgacact tgggttgtt gttgcatca gttcagacg 
      agtcgaagt gtgtcaggt gcgcgttac ctcccttct 
      gatgatgtc aaacaggac gaaagaatc aaactcttg 
      aatgaggaa agtaccgaa agtaccgaa actaccgac 
      gaaccaatg acggtacta ccaaccacc aaacacaaa 
      acttctact gaaagtttg atcgataag ctagaccgt 
      gtccagtcg gatttatcg agcagtgtt ttcaatggt 
      ggtttgaac aatatagcg gttataacc gaaatggcc 
      acaaccaca cccccacca cctaaacca gtggtccaa 
      atccgaaaa ttttcacct tttgcacgt ccttccacc 
      acaccaaga accaggaaa attgaaatt gtgaaacag 
      gatgatttg accggtttg ctaccacct gggtttaag 
      cccaagtac cagaaccgc aaaactacg gttaaaccg 
      gatttggag ttaccacaa ggtgatgaa ccaccgaaa 
      attggtcgt tcgtcgggt atttcaaac aaaaccaaa 
      ataacagta caggatgta agcccattt ttaccacct 
      ggctacaaa agtaccaaa gaccaggat gatagtacc 
      aaacttttg gccgaaatt ttggggaaa gttaagccc 
      aaaatcgtt aaagttgac gatgtttcg gccttttta 
      ccgcctggc tataaacct acttctggt actactagt 
      actagtacc accaccacc aaatcggtt gatggaatt 
      ttgtcaaag gctaaaccg gttgatgat atcagcgcc 
      ttgttacca cctggatat aagtctagt actagtact 
      accaagcct aaacctagc attgagggg gtactagct 
      cacgcgaaa ccggttgat gatatcagt gctttgtta 
      ccacctggt tataaacca tctagtacc gctaaacct 
      gttaaacca aaaggggtt gatgggtta ttagcccag 
      gctaaacca gtagacatt agtgccttc ctaccaccg 
      ggttataag gcacgaacg acgcaaaaa cccctaaca 
      acgcctgag aaagtacca tctagtttg ctaccacct 
      gggtacaaa ctacgcgaa actactact actagtagt 
      actactagt accaccact accacgacc agtaaacca 
      agcggtttt aagatcgtt ttcccaagc cgacctggt 
      ggtacccac cgtaaatcc ccaaataga attaccacc 
      cctcctagt accatgacc gaggaacca cgtgccacg 
      gcaccaaca attcacaaa gggtggccc tcacgggcg 
      actactgaa ttcacaggg tggccaacc ccttcaacc 
      actcccatt tcaattgaa aagttactt gaagctgcg 
      agaaccgcg gcaacaagt accacccca gctgtggac 
      agtaccacc agtaccaca actagtacc accacgacc 
      acgaccacc acaaccccc aaacccaca actccgggg 
      gtgtgtacg ggcgagtgt gaccttgct ggtaccatc 
      aagctagtc ggtggtgcc aaatgggta ccagaactt 
      ctagaccgg aatacgaaa gagtggcag attttggca 
      aacgaagtc cagacacag cttgaggat gtttttggc 
      cgctcaaac gtgctgaat aagtggtac cgcaagata 
      cggatcgat gggtttagt gaagggtcg gtccttgtt 
      gactatttg gtcgaatta aatgatctg gggcggaaa 
      atcgataca caagagatc aaaaaactg tttcatgat 
      tcgctcgac gatggtgcg acgaatcgt gagggaaaa 
      tcgttgaac gaaacgtcg aaaatggct ttagggaag 
      tttacagtt gatccgaaa ttcactgat tttgtcgtt 
      ttgcccaaa caagtttac ccaactgtg ggctatgct 
      gaggataat gtgctattg ccccaatgg gcgattgct 
      gtcattgtt attggttta gcgtcgctt ctttttgtc 
      ataattttc ggtgttacg gtgttggtc aatcggcag 
      aaaaatgcc aaaaagaag gctcctgtt tcactgact 
      gaggatatg ttgaatgaa ttgaacaag aaccatatg 
      ggtgggttt gacaattat ggtgccgat gatttgtac 
      aatatggag gatgtgtgg aatgataga ggctttgag 
      cctaagctc gccaaaaag cgctcctcc ggttctctg 
      cacgacaac agcatgtcc aacctttac gacagctgg 
      cgttccgaa tggaacggt tactattac aacgcctac 
      tacggcaac aacaaccct ggaagtagc agcagccat 
      tacggccgt cgaagatcc gactacgac acgaatttt 
      tga
    mRNA sequence
    • >TC011099 RA
      attacaact ccactcgtc ttcaaaaat gtttaaaat 
      cctaatttc ttgcctctt gttcgtcca tcggttgtt 
      gtgttcttg tcgttactt tcacgcgaa gtggtgcag 
      ttatgtgat ggggtgctt tctgacagt gggggtgct 
      cttctcaac cctttgtct atttgctgc aggtcagga 
      gtacatcat cacgggccc tgcggacac ccaattcat 
      ccccaatta tcgccagac ccccgactt caacccctt 
      cagacccaa cttccaaca cgtggactc ctacctccc 
      tcaaaggga cctccaaac gtcccacct cccctacca 
      cttgcaaca aaccctccc gcagtaccg tgccaccac 
      gaaaacacc cccaaggta ctcctacca accccagtt 
      cgactatcg aagctcaca accagcgcc attccgcac 
      ccaggagca aatagtacc atcaaccca tcggccaaa 
      ctactccaa cttgttccc acactccca tcgtaaccc 
      acaaactga aaccttcct tggggaaat cacacttca 
      aagtccaga agacttcaa acaggagat agacacgac 
      tgagaaaag caacgttgt gacgattgg aggccgagg 
      gaatttttt gaaccacag gacacgaat ttttgctcc 
      tgataccgt caaaaaagt accacttgt gagggagta 
      ctatgttaa caaaaatgg gaatgtttt tagtaccac 
      acgagctcc cataaccca aaaaacgac aactgaaag 
      tttgagacg aaaaactac aagtggtac tactagtac 
      tactattga aagtactac taggaggag caggaacgg 
      gttagattt tgagtcact tttggagga accgatcaa 
      aaattccaa caccaatga tgatgtggt cgaagttgt 
      caagtcaaa aagtgtcga aatgacaac tttgaatag 
      tcacaatga gagtaccac cgattttga ctatgatac 
      aactcttga tccgtcagt agactacga taaagacga 
      aggagaaga agaagaaga agaagatcc aatcaaaca 
      tctggaaga gttaaggaa gcaacatcc tgaagaaga 
      agattacgt tagtgaaga agaggaaac tccgaattc 
      tgaagaaac caacaatgt tcgtgacga agattatga 
      agaagaaga aggtaccac cactagtac cgttagaac 
      tacttctag tactactcc tagtaccat taaaacaac 
      tattgctac tcctagtac tattagaac tactgatgc 
      tactcctag tactaccac tgaagaagt accaaccac 
      tcgcaaaac tgaggctct tttggccga accggagac 
      cgtaatttc ggtcgtaac gactaaaag tgtggttaa 
      caacaccat ccttccgac agctacacc tttacctaa 
      ctctaccat aacttcaga gaatagtac cgacacttg 
      ggttgttgt tgcatcagt tcagacgag tcgaagtgt 
      gtcaggtgc gcgttacct cccttctga tgatgtcaa 
      acaggacga aagaatcaa actcttgaa tgaggaaag 
      taccgaaag taccgaaac taccgacga accaatgac 
      ggtactacc aaccaccaa acacaaaac ttctactga 
      aagtttgat cgataagct agaccgtgt ccagtcgga 
      tttatcgag cagtgtttt caatggtgg tttgaacaa 
      tatagcggt tataaccga aatggccac aaccacacc 
      cccaccacc taaaccagt ggtccaaat ccgaaaatt 
      ttcaccttt tgcacgtcc ttccaccac accaagaac 
      caggaaaat tgaaattgt gaaacagga tgatttgac 
      cggtttgct accacctgg gtttaagcc caagtacca 
      gaaccgcaa aactacggt taaaccgga tttggagtt 
      accacaagg tgatgaacc accgaaaat tggtcgttc 
      gtcgggtat ttcaaacaa aaccaaaat aacagtaca 
      ggatgtaag cccattttt accacctgg ctacaaaag 
      taccaaaga ccaggatga tagtaccaa acttttggc 
      cgaaatttt ggggaaagt taagcccaa aatcgttaa 
      agttgacga tgtttcggc ctttttacc gcctggcta 
      taaacctac ttctggtac tactagtac tagtaccac 
      caccaccaa atcggttga tggaatttt gtcaaaggc 
      taaaccggt tgatgatat cagcgcctt gttaccacc 
      tggatataa gtctagtac tagtactac caagcctaa 
      acctagcat tgagggggt actagctca cgcgaaacc 
      ggttgatga tatcagtgc tttgttacc acctggtta 
      taaaccatc tagtaccgc taaacctgt taaaccaaa 
      aggggttga tgggttatt agcccaggc taaaccagt 
      agacattag tgccttcct accaccggg ttataaggc 
      acgaacgac gcaaaaacc cctaacaac gcctgagaa 
      agtaccatc tagtttgct accacctgg gtacaaact 
      acgcgaaac tactactac tagtagtac tactagtac 
      caccactac cacgaccag taaaccaag cggttttaa 
      gatcgtttt cccaagccg acctggtgg tacccaccg 
      taaatcccc aaatagaat taccacccc tcctagtac 
      catgaccga ggaaccacg tgccacggc accaacaat 
      tcacaaagg gtggccctc acgggcgac tactgaatt 
      cacagggtg gccaacccc ttcaaccac tcccatttc 
      aattgaaaa gttacttga agctgcgag aaccgcggc 
      aacaagtac caccccagc tgtggacag taccaccag 
      taccacaac tagtaccac cacgaccac gaccaccac 
      aacccccaa acccacaac tccgggggt gtgtacggg 
      cgagtgtga ccttgctgg taccatcaa gctagtcgg 
      tggtgccaa atgggtacc agaacttct agaccggaa 
      tacgaaaga gtggcagat tttggcaaa cgaagtcca 
      gacacagct tgaggatgt ttttggccg ctcaaacgt 
      gctgaataa gtggtaccg caagatacg gatcgatgg 
      gtttagtga agggtcggt ccttgttga ctatttggt 
      cgaattaaa tgatctggg gcggaaaat cgatacaca 
      agagatcaa aaaactgtt tcatgattc gctcgacga 
      tggtgcgac gaatcgtga gggaaaatc gttgaacga 
      aacgtcgaa aatggcttt agggaagtt tacagttga 
      tccgaaatt cactgattt tgtcgtttt gcccaaaca 
      agtttaccc aactgtggg ctatgctga ggataatgt 
      gctattgcc ccaatgggc gattgctgt cattgttat 
      tggtttagc gtcgcttct ttttgtcat aattttcgg 
      tgttacggt gttggtcaa tcggcagaa aaatgccaa 
      aaagaaggc tcctgtttc actgactga ggatatgtt 
      gaatgaatt gaacaagaa ccatatggg tgggtttga 
      caattatgg tgccgatga tttgtacaa tatggagga 
      tgtgtggaa tgatagagg ctttgagcc taagctcgc 
      caaaaagcg ctcctccgg ttctctgca cgacaacag 
      catgtccaa cctttacga cagctggcg ttccgaatg 
      gaacggtta ctattacaa cgcctacta cggcaacaa 
      caaccctgg aagtagcag cagccatta cggccgtcg 
      aagatccga ctacgacac gaatttttg aaaagattc 
      gccaaactg ccaatcaga atccgaatc agtacagtc 
      cgttgattt tcatgcaca caaacacct aaacaaaca 
      gggtttttc ccccgattg gttttgctc aaatcccgt 
      ccttccccg agaatgtac agatatcga taaataatt 
      gccaaaatg ctagtgatg aaaatcgag cggggctgt 
      agtagtttt ccaggtttt tctagtcaa caaacagcc 
      tactagcaa tggccaaag gctgtgaaa gcaacctat 
      cgagacttt attatattg aacatcgat gacgttgag 
      cttgcgcgc gatttttgc cgtttttat tacgtgttt 
      gcttccgtt ggagcaaag ttttttgga aacttttcg 
      aacgtgcga atgtgtagt tcatcccgt tttttcaac 
      tgtatataa gtgtaaata tatcgtaag attatatct 
      attttccca ttacctagg ctagacgca ttcaacatt 
      taaattatt gtaaatgta gctgttaag gtgttaaat 
      aaatcatta taaataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY