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Gene information
General information
TC002890 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7126796..7131541
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002890
    iBeetle screen iB_06950

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    only 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002890 RA
      MAEIYRCEK AAVEKLQAV PEPEWILDR 
      TGTGPLYWR RESPECNSR VKFRFDVEV 
      KEFQRSAHE FEDLEWSRC IEESNTLTM 
      GLACIICIT VTVMLPWYI MIGTY
    CDS sequence
    • >TC002890 RA
      atggcggaa atttatcgt tgcgaaaaa gccgctgtg 
      gagaagctg caagccgtt ccggaaccg gagtggatt 
      ctggacagg accggcacc gggcccttg tattggcgg 
      agagagtcc ccggaatgc aattccaga gtaaaattc 
      cggttcgac gtggaagtc aaggagttc cagcgcagt 
      gctcacgaa ttcgaagac ttggaatgg tcgcgatgc 
      atcgaagaa tccaacact ctaaccatg ggtctggcg 
      tgcatcatt tgcatcacc gtaacagtg atgctccct 
      tggtacatc atgatcgga acctattga
    mRNA sequence
    • >TC002890 RA
      tattaaacg tattaagcc gcttgcgca tgtctgata 
      ggtaatgtg tcaatggtt tatctagtt gttgccgat 
      gtaagttca aatgttgct gcgaaaaca tttgtgcta 
      aattttgcc caacttttg agcgacagg tctggacat 
      tagtcactg caagttgta tcaaaaagt ggaagaaat 
      cgagccaaa cagtcatcc aagaggacg gggtcgccg 
      aaaattaaa taaaaaagt ccgacagag aacaataga 
      ccgtagctt ataatgggc agtaattcg tgattacaa 
      tacaaactt tccgactcg ataaaagtg tccatataa 
      aaaatcgca gcaagctgc cgtgttaaa gaacaataa 
      aagcaagtt tttaatgag aaaataaaa ccatggcgg 
      aaatttatc gttgcgaaa aagccgctg tggagaagc 
      tgcaagccg ttccggaac cggagtgga ttctggaca 
      ggaccggca ccgggccct tgtattggc ggagagagt 
      ccccggaat gcaattcca gagtaaaat tccggttcg 
      acgtggaag tcaaggagt tccagcgca gtgctcacg 
      aattcgaag acttggaat ggtcgcgat gcatcgaag 
      aatccaaca ctctaacca tgggtctgg cgtgcatca 
      tttgcatca ccgtaacag tgatgctcc cttggtaca 
      tcatgatcg gaacctatt gaaaatctt tagtcggac 
      atctgtgat tgaccgaca ggactttga actgttgac 
      ttgttaaag gtcaaggag tgattgtga tattaaata 
      tttattgag ggtattaca tacctacgt tgtgagaaa 
      aagtgatat ttaatttag cctttgtta catcgcttc 
      cgatgtaaa taaaattaa gttcatttt gtttgtacg 
      tgggcccga tttgaaaat gtttggttg ggacaaatg 
      ttggctctg ggatataga accgtttag gttctttga 
      agccatttg ttttattac gggagaaat aaacaaaac 
      gacaaacac tagatgcac atatgaatc tatgacgga 
      aatgcaaaa caggttcag taaaatcga ctccaaaag 
      catttgtag tttagtaaa aaggagcgc ttcttccac 
      acaagtttg caaattttc tggcgatca cccacgaac 
      ttgtggctc ctaaaagac tgcagatcc actagtata 
      ttacgtaat tttcgataa agattagat aactccaat 
      tcgaaaata actttgaat aatcaaact tttatttta 
      acgtcacaa attaatttt taatataag actacagct 
      gtatttcag aaaggggta aagtttatt ttagaagat 
      attgtaatt caggctagt aaactgtaa ttcagaaat 
      aaaatttgt aatattctg ggtttaaaa tttaaaatt 
      acaaaatac cacaaaaat caaaactat gttcttaat 
      tactacttc gtttttttg gttggctta ggtaaaagt 
      tttactttc aattgaaaa aaacacaaa cttctgcat 
      tttgatttt attctgtac atctgtaca gtcatcaat 
      aaaaagcat gacacccta aaaccgcaa atttttcga 
      tatgaattt aaactaaaa aagtcatct ggcacgaaa 
      agttgataa ctgttgact gctttcaag gggaaaaaa 
      agggaaaaa atcaattat tacatttct catctcttt 
      tttttagtt tataaagac atcaaaaat taggtctaa 
      ctaaataag aaatttaat aaaaaactt ttttttcta 
      aatgtgtag aatgtgact ttttcccgt gtactcttc 
      agttaccta cttaaattt attaattaa acttttgta 
      tccttgata ggaaagctc cgtacaaac aaaataata 
      actaaaatc tctgattac agtggcaga aaaaaataa 
      aacttttag tagttttct agtcataaa atatacctt 
      tttgtaact tatataaga catcccaga acttgactg 
      ggtaccaaa tcatcctgt atatagctg catcaatgt 
      ctaaatctg gtgattttt cctggtgtg tacgcatat 
      tgagtgagt tgtttaaaa acattgttt atatgataa 
      gagaaaaaa ttgtctaca ataaaatta actttttaa 
      t
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