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Gene information
General information
TC002909 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6663955..6703667
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002909
      iBeetle screen iB_06945

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      labrum not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      labrum shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      antenna pedicellus sometimes not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002909 RA
        MCCKSRGAL SRLGVLWGL LALATLLIA FVSGSWLYT 
        KEPVTLPNT QVTTSISFR IGLWRVCPS VKRVNSSLH 
        NPSPACSLV KYAGWEDLS PSDIGIWTS LDLAQVFIS 
        RMRSCTVLT GSAVVLLLL ASFFSLLGH CNSDNKTII 
        ACGFFILGG LSLGTGLVV FASALSETL MEITQYHKE 
        PPSGPVYDY RYGWCFFTA GAAFIMTKM AAVFSLTGY 
        LNRFPSVDE MVREMVPGA ERKLREHQR LSSEYLVRH 
        AAPSPRQQY DVISTNRPK YEAECGPLL NKTPPDVCT 
        TNKCVDFGV TDLNSSVLM SGVIEQNYS GGGHVMAGQ 
        TIPITIKNH TSTAPLSFS PANKYGTMP GTTLHQGFL 
        GVAEIGSSS SNSSSGYCS KSKTLQHQR PRKKCVKIE 
        SFQTPDAGF ADFGGKRSN TLSYSGSAV
      CDS sequence
      • >TC002909 RA
        atgtgttgc aagtcgcgg ggtgctctc tctagactg 
        ggtgtcctg tggggactt ctggcgctg gccactcta 
        cttatcgct tttgttagt ggctcttgg ctctacact 
        aaagagccg gtcacactg cctaacacc caagtcacc 
        acttccatc agctttcgc atcggctta tggagggtc 
        tgtccttcc gtcaaaagg gtcaactcc tcgctacac 
        aatccctcg cctgcatgt tctctggtc aagtacgcc 
        ggctgggag gacttgagt ccctcggac atcggaatt 
        tggacctct ttggacctc gctcaagtc ttcatctcg 
        cggatgagg tcatgcact gttttaact ggatcggct 
        gtagttttg ctattactc gcatccttc ttctcactt 
        ctcggccac tgtaacagc gacaataaa acaataata 
        gcgtgcgga ttcttcatt ttaggaggg ctttctttg 
        ggaacgggt ctggtggtt tttgcatct gctctgtca 
        gagactctg atggaaata acacaatat cacaaggaa 
        cctccctcc ggccccgtc tacgactat cgttacgga 
        tggtgtttc ttcacggcg ggggcggct tttatcatg 
        acaaaaatg gctgcagtc ttctctctt acaggatat 
        ctcaacaga tttccttct gtcgatgaa atggtacgg 
        gaaatggtc ccgggggcg gagcgcaag ttgcgggag 
        caccagcgc ctctccagc gagtacctg gtgcggcac 
        gcggcgccg tcccccagg cagcagtac gacgtcatc 
        tccacaaac cgtcccaag tacgaggcc gagtgcggc 
        cccctgctc aacaagacc cctccggac gtgtgcacc 
        accaacaag tgcgtcgat ttcggtgtg acggacctc 
        aacagtagt gtgctaatg tctggtgtg atcgagcaa 
        aactacagt ggcggaggg cacgtgatg gccggccag 
        accattccc atcaccatc aaaaaccac acttcgacg 
        gcgccgctc tccttctcg cccgccaat aagtacgga 
        acaatgcca ggaacgacg cttcatcag ggattcctg 
        ggcgtcgcc gaaattgga agttccagt tcgaacagt 
        tcgagtgga tactgttcc aaaagtaag actttacaa 
        caccaaagg ccgaggaag aagtgtgtt aaaattgaa 
        agtttccaa actcctgat gccgggttc gcagacttt 
        ggaggcaag aggtcgaat acattaagt tactccggg 
        agcgcggta tga
      mRNA sequence
      • >TC002909 RA
        caagttcga acgaaattt cagagcttt aacgatacg 
        tgatgagtg ccacgtttg cttaaatta aggtgaaat 
        tacacactt gggtgactt gaactttta tgcaacatt 
        agtcttaga gtgatcatg ccttcgaga gctgatcga 
        ggccaaaca aatccttca ccagatgtt attccttct 
        cagcgtgaa aacatacaa gtggaacgt gtgcatcac 
        agtaaaccg aaatgtata caagaccag tcgaggctt 
        tagatgcct gccatattt tagcgcgaa caataagtg 
        ccataaaac agtctgcca caataagcg gtgcctgaa 
        acggatcta atgtaaagt tttcgcttt gcggtgcat 
        tgtgatgtg ttgcaagtc gcggggtgc tctctctag 
        actgggtgt cctgtgggg acttctggc gctggccac 
        tctacttat cgcttttgt tagtggctc ttggctcta 
        cactaaaga gccggtcac actgcctaa cacccaagt 
        caccacttc catcagctt tcgcatcgg cttatggag 
        ggtctgtcc ttccgtcaa aagggtcaa ctcctcgct 
        acacaatcc ctcgcctgc atgttctct ggtcaagta 
        cgccggctg ggaggactt gagtccctc ggacatcgg 
        aatttggac ctctttgga cctcgctca agtcttcat 
        ctcgcggat gaggtcatg cactgtttt aactggatc 
        ggctgtagt tttgctatt actcgcatc cttcttctc 
        acttctcgg ccactgtaa cagcgacaa taaaacaat 
        aatagcgtg cggattctt cattttagg agggctttc 
        tttgggaac gggtctggt ggtttttgc atctgctct 
        gtcagagac tctgatgga aataacaca atatcacaa 
        ggaacctcc ctccggccc cgtctacga ctatcgtta 
        cggatggtg tttcttcac ggcgggggc ggcttttat 
        catgacaaa aatggctgc agtcttctc tcttacagg 
        atatctcaa cagatttcc ttctgtcga tgaaatggt 
        acgggaaat ggtcccggg ggcggagcg caagttgcg 
        ggagcacca gcgcctctc cagcgagta cctggtgcg 
        gcacgcggc gccgtcccc caggcagca gtacgacgt 
        catctccac aaaccgtcc caagtacga ggccgagtg 
        cggccccct gctcaacaa gacccctcc ggacgtgtg 
        caccaccaa caagtgcgt cgatttcgg tgtgacgga 
        cctcaacag tagtgtgct aatgtctgg tgtgatcga 
        gcaaaacta cagtggcgg agggcacgt gatggccgg 
        ccagaccat tcccatcac catcaaaaa ccacacttc 
        gacggcgcc gctctcctt ctcgcccgc caataagta 
        cggaacaat gccaggaac gacgcttca tcagggatt 
        cctgggcgt cgccgaaat tggaagttc cagttcgaa 
        cagttcgag tggatactg ttccaaaag taagacttt 
        acaacacca aaggccgag gaagaagtg tgttaaaat 
        tgaaagttt ccaaactcc tgatgccgg gttcgcaga 
        ctttggagg caagaggtc gaatacatt aagttactc 
        cgggagcgc ggtatgacg agcgaaagc tattatgga 
        aaaatattc taaaacttc cctagggca cactggaaa 
        tttacactt gcattgtaa ataataagg gcgatcgga 
        aagtttgct aatgaaaat ccctgatta aagcgaggg 
        atttttata gactgacac taaattgtt tacaatgca 
        gggttcttt ttggttcaa gggcgcaga aggactagg 
        ataaagtca caataacac cagttacgt tacaaataa 
        atataaaaa ttactattt atttacaaa cttttaaca 
        aaactgaaa taaaatcca aatactata tcaaaataa 
        tagcgacaa tctttcaac cccgttaac taacacaga 
        gccggatta caactatgg aagccctag gacaatttt 
        tattacaca gtgaaacaa aatactcca gacaaaaaa 
        agaaaaaaa atatattat tataatata ataaatata 
        ttaatatat aaagatata ttatcgacc tgtaaatgg 
        ttacattcg atttaggat aaaacgata cattaaaaa 
        cattacaga actctaaat aattgacat ttgcgaagt 
        ttatttaac ggtcattta aatgtattt tttgcgtat 
        tgttcttaa aataaatga ggttttatt aaaaaaaag 
        taagaacta agtgtcaac actataaaa taaaagtaa 
        aaattaaaa atgaaataa taaaggata aaataataa 
        tttttaaac ataaatgcg ctagagttc aataactat 
        cacaattca aaaactgtt acaactagt ataaatatc 
        tacaaaaaa aatattttt atatttagg aaaaattta 
        atataaatt aataaataa ataaataaa taaataaaa 
        acagaacct aaattagta actaaatta acccacatc 
        aaaaatgat aacagatta gagtggtaa taactaata 
        atgtttgtt tcttcacaa acatgttag tttcgagct 
        caggtttga aaaagttta tgtaatgag gaaagcatc 
        ctaatttgt tgctaagcg tgagttttt ttttagaaa 
        ttacgataa aatgcacat ttaaaacaa tatgcaaaa 
        taggggtaa agcttataa cttaaaata aaacacttg 
        tttctactt tcttgagcc tcttcagaa ataatccga 
        ctccaaaaa attccgtca gtcaatcca tcaagataa 
        taaagatag gtaatagtc ctaataaaa aaatattat 
        tgaaacgta ataactaat aacaaaaat gtaaacaac 
        atccaagac tacaagtca ttgcagcta aaaccaacc 
        ttaatacta ctttaagta tatcctact ttgaaaaaa 
        tgactttta gtgtgaaag aaaacgaaa attttttcc 
        tgaaaaaac cactaatca aaacataaa ctaaataac 
        atgacaaac acataataa agcatctaa atcaaaaga 
        atatgacaa tgattcaac ttaaataaa tgatttggt 
        ggcaaaaga gcaaaataa taaatccga gaatttgct 
        aagcgcaaa acaaaaaga agacagata aatgtaatg 
        caaaaattt tatcgaaaa ctacattaa aataaaata 
        ccaagttgc ac
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