Status
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Gene information
General information
TC011105 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 275446..314897
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011105
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06941

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: spineless
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    antenna not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna potentially transformed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: at antenna locus
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011105 RA
      MELCVLKPT PKDGVTKSN PSKRHRERL NAELDTLAS 
      LLPFEQNIL SKLDRLSIL RLSVSYLRT KSYFQVVMH 
      KDKDDGGLH RTRDLTAFD HTPLDGEMF LQALNGFLM 
      ILTCEGEVF FATHSIEGY LGFHQSDIV HQSVYELVH 
      SEDREELQR QLMWNSFLP PESANMGLQ DVLLPENCH 
      LLERSFTVR FRCLLDNTS GFLRLDIRG RVKVLHGQN 
      RKSDEAPLA LFAICTPFG PPSLLEIPQ KEVMFKSKH 
      KLDLALVSM DQRGKMLLG YSDSELATM GGYDLVHYD 
      DLAYVASAH QELLKTGAS GMIAYRFQT KDGQWQWLQ 
      TSSRLVYKN SKPDFVIST HRPLMEEEG RDLLGKRTM 
      DFKVSYLDA GLPNSYFSE SDLLSTPTA QQHNLTPSR 
      VNRRYKTQL RDFLSTCRT KRKLSNSGQ VTPPTNTTV 
      TASVSSPLP DYIAPDYNM YSTSYPTPT PDLSYMPHS 
      NFYPTTPLD NRYLTTPDN LFHQYRPLG TYYPDYHST 
      PSYNGFPYE PTPTNYPCQ QSYPNSWPY SVSPSSGII 
      QPVQVMELG KPPKIPKIE APKMEEREY VGEAPRQTV 
      LMWGSNPPT TSDESSELT NQWNGEEKT EQNEDSHPH 
      HNPHHHHNS RVVMVL
    CDS sequence
    • >TC011105 RA
      atggaatta tgtgtcctg aagccaacg cccaaagac 
      ggcgtgacg aagagcaac cctagcaaa cgtcaccgt 
      gagcgtctc aacgccgaa ttggacaca ttggccagt 
      ttgttgcct tttgagcaa aatatcctc tcgaaattg 
      gaccgactg tcgatcctg cgactctcc gtcagctat 
      ctacgcacc aaaagctac tttcaagtt gttatgcac 
      aaagataag gacgatggc ggcctacat cggacccga 
      gatctcacc gccttcgac cacactccc ctagatggc 
      gaaatgttc ctccaggcc ttgaatggg ttcctcatg 
      attctgacg tgcgaaggt gaagttttt ttcgcaacg 
      cacagcatt gaagggtac ttagggttc caccagtct 
      gatattgtc caccaatcc gtctatgaa ctcgtccat 
      tcagaagat agagaagaa ttacaaaga cagttaatg 
      tggaactcg tttcttcca cccgaatcg gctaatatg 
      ggccttcaa gatgttctc ctacctgaa aattgtcac 
      ttgttggag aggagcttc acagtgcgc ttccgatgt 
      ctcctagac aatacttcc ggttttttg cgtttagat 
      ataagaggg cgtgttaag gtactccac ggccaaaat 
      cgtaagtca gatgaggca cctttggcc ctttttgcc 
      atttgtacc ccttttgga ccaccctcc cttctggag 
      ataccgcag aaggaagtc atgtttaag agtaagcac 
      aagttggac ttggccttg gtttcgatg gatcagaga 
      ggaaaaatg ttactgggg tattctgat tcggagttg 
      gccacgatg ggagggtac gacctagtg cactacgat 
      gatttggcc tacgtggca agtgcacac caggaatta 
      ctcaaaact ggcgcttct ggcatgata gcgtaccgt 
      ttccaaaca aaagacggt caatggcag tggctccaa 
      accagttct cgtctagtc tacaaaaac tccaaaccg 
      gattttgtg attagcacc caccggcct ttaatggag 
      gaagaaggt cgcgacctc ctcggcaaa cgtacaatg 
      gatttcaaa gtgagttac ttagacgcc ggtcttcca 
      aacagctac ttctccgaa agcgacctc ctctccacc 
      cccactgct caacaacac aacctgacc ccatctcgc 
      gtcaaccgt cgctacaag acccaactt cgcgatttt 
      ttatcaaca tgtcgcaca aaacgcaaa ctatcaaac 
      tccggtcaa gtaactcct cccactaac acaacagtc 
      accgcctcc gtgtccagc cccctcccc gactacatc 
      gcccctgac tacaacatg tacagcacc tcctatccc 
      acgcccacg cccgacctc tcctacatg ccccactcc 
      aacttctac ccgaccacg ccccttgac aaccggtac 
      ttgaccacg cccgacaac ctcttccac caataccgc 
      cctctcggc acctactac cccgactat cactccacc 
      ccctcctac aacggcttt ccctacgag cccaccccc 
      accaactac ccctgccag cagtcctac cccaactcc 
      tggccctac tcggtgagc ccttcgtca gggatcatc 
      caaccagtg caagtcatg gagttgggg aagccccca 
      aaaatccca aaaatcgag gcccccaaa atggaggag 
      cgggagtat gtgggggag gcgccccgc cagacggtg 
      ctcatgtgg gggtcgaac ccccccacc accagtgac 
      gaaagcagc gagctgacc aatcagtgg aacggcgag 
      gagaagact gagcagaac gaggacagt catccccac 
      cacaacccc caccatcat cacaattcg cgcgttgtc 
      atggtgttg tga
    mRNA sequence
    • >TC011105 RA
      cttggtata aatgcacaa tggaattat gtgtcctga 
      agccaacgc ccaaagacg gcgtgacga agagcaacc 
      ctagcaaac gtcaccgtg agcgtctca acgccgaat 
      tggacacat tggccagtt tgttgcctt ttgagcaaa 
      atatcctct cgaaattgg accgactgt cgatcctgc 
      gactctccg tcagctatc tacgcacca aaagctact 
      ttcaagttg ttatgcaca aagataagg acgatggcg 
      gcctacatc ggacccgag atctcaccg ccttcgacc 
      acactcccc tagatggcg aaatgttcc tccaggcct 
      tgaatgggt tcctcatga ttctgacgt gcgaaggtg 
      aagtttttt tcgcaacgc acagcattg aagggtact 
      tagggttcc accagtctg atattgtcc accaatccg 
      tctatgaac tcgtccatt cagaagata gagaagaat 
      tacaaagac agttaatgt ggaactcgt ttcttccac 
      ccgaatcgg ctaatatgg gccttcaag atgttctcc 
      tacctgaaa attgtcact tgttggaga ggagcttca 
      cagtgcgct tccgatgtc tcctagaca atacttccg 
      gttttttgc gtttagata taagagggc gtgttaagg 
      tactccacg gccaaaatc gtaagtcag atgaggcac 
      ctttggccc tttttgcca tttgtaccc cttttggac 
      caccctccc ttctggaga taccgcaga aggaagtca 
      tgtttaaga gtaagcaca agttggact tggccttgg 
      tttcgatgg atcagagag gaaaaatgt tactggggt 
      attctgatt cggagttgg ccacgatgg gagggtacg 
      acctagtgc actacgatg atttggcct acgtggcaa 
      gtgcacacc aggaattac tcaaaactg gcgcttctg 
      gcatgatag cgtaccgtt tccaaacaa aagacggtc 
      aatggcagt ggctccaaa ccagttctc gtctagtct 
      acaaaaact ccaaaccgg attttgtga ttagcaccc 
      accggcctt taatggagg aagaaggtc gcgacctcc 
      tcggcaaac gtacaatgg atttcaaag tgagttact 
      tagacgccg gtcttccaa acagctact tctccgaaa 
      gcgacctcc tctccaccc ccactgctc aacaacaca 
      acctgaccc catctcgcg tcaaccgtc gctacaaga 
      cccaacttc gcgattttt tatcaacat gtcgcacaa 
      aacgcaaac tatcaaact ccggtcaag taactcctc 
      ccactaaca caacagtca ccgcctccg tgtccagcc 
      ccctccccg actacatcg cccctgact acaacatgt 
      acagcacct cctatccca cgcccacgc ccgacctct 
      cctacatgc cccactcca acttctacc cgaccacgc 
      cccttgaca accggtact tgaccacgc ccgacaacc 
      tcttccacc aataccgcc ctctcggca cctactacc 
      ccgactatc actccaccc cctcctaca acggctttc 
      cctacgagc ccaccccca ccaactacc cctgccagc 
      agtcctacc ccaactcct ggccctact cggtgagcc 
      cttcgtcag ggatcatcc aaccagtgc aagtcatgg 
      agttgggga agcccccaa aaatcccaa aaatcgagg 
      cccccaaaa tggaggagc gggagtatg tgggggagg 
      cgccccgcc agacggtgc tcatgtggg ggtcgaacc 
      cccccacca ccagtgacg aaagcagcg agctgacca 
      atcagtgga acggcgagg agaagactg agcagaacg 
      aggacagtc atccccacc acaaccccc accatcatc 
      acaattcgc gcgttgtca tggtgttgt gatgaatgt 
      ggaggggga ctcgtgtat ataactcca gcacacgta 
      ttttcctcc gcgcaaaca tatcttcct gtgtttttg 
      ttatattat tgtcaatgt cttcgatta ggacctaac 
      ctttctttt tttttggtt gttatttat ttttagttt 
      ttacgcagt gaaaacatg ctagtcaac tggttacat 
      acttgtata tgttgtaca ttattattt atgaatgta 
      gtaatataa tgagtagat tagtccatt gttcgattg 
      tttttctat aaatattta caaaacaaa acgaaatat 
      ataacttca tgtacggtt gagttttag ggacgagca 
      aacaacatt ccattcgag atctttcaa ttgtacatt 
      aacgtttaa gaatcttgt attacctgt aacgtgtat 
      gaaaattat ataatgata aaataaatt atattacaa 
      tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY