Status
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Gene information
General information
TC011105 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 275446..314897
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011105
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06941

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: spineless
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     antenna not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna potentially transformed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: at antenna locus
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011105 RA
      MELCVLKPT PKDGVTKSN PSKRHRERL NAELDTLAS 
LLPFEQNIL SKLDRLSIL RLSVSYLRT KSYFQVVMH 
KDKDDGGLH RTRDLTAFD HTPLDGEMF LQALNGFLM 
ILTCEGEVF FATHSIEGY LGFHQSDIV HQSVYELVH 
SEDREELQR QLMWNSFLP PESANMGLQ DVLLPENCH 
LLERSFTVR FRCLLDNTS GFLRLDIRG RVKVLHGQN 
RKSDEAPLA LFAICTPFG PPSLLEIPQ KEVMFKSKH 
KLDLALVSM DQRGKMLLG YSDSELATM GGYDLVHYD 
DLAYVASAH QELLKTGAS GMIAYRFQT KDGQWQWLQ 
TSSRLVYKN SKPDFVIST HRPLMEEEG RDLLGKRTM 
DFKVSYLDA GLPNSYFSE SDLLSTPTA QQHNLTPSR 
VNRRYKTQL RDFLSTCRT KRKLSNSGQ VTPPTNTTV 
TASVSSPLP DYIAPDYNM YSTSYPTPT PDLSYMPHS 
NFYPTTPLD NRYLTTPDN LFHQYRPLG TYYPDYHST 
PSYNGFPYE PTPTNYPCQ QSYPNSWPY SVSPSSGII 
QPVQVMELG KPPKIPKIE APKMEEREY VGEAPRQTV 
LMWGSNPPT TSDESSELT NQWNGEEKT EQNEDSHPH 
HNPHHHHNS RVVMVL
    CDS sequence
    • >TC011105 RA
      atggaatta tgtgtcctg aagccaacg cccaaagac 
ggcgtgacg aagagcaac cctagcaaa cgtcaccgt 
gagcgtctc aacgccgaa ttggacaca ttggccagt 
ttgttgcct tttgagcaa aatatcctc tcgaaattg 
gaccgactg tcgatcctg cgactctcc gtcagctat 
ctacgcacc aaaagctac tttcaagtt gttatgcac 
aaagataag gacgatggc ggcctacat cggacccga 
gatctcacc gccttcgac cacactccc ctagatggc 
gaaatgttc ctccaggcc ttgaatggg ttcctcatg 
attctgacg tgcgaaggt gaagttttt ttcgcaacg 
cacagcatt gaagggtac ttagggttc caccagtct 
gatattgtc caccaatcc gtctatgaa ctcgtccat 
tcagaagat agagaagaa ttacaaaga cagttaatg 
tggaactcg tttcttcca cccgaatcg gctaatatg 
ggccttcaa gatgttctc ctacctgaa aattgtcac 
ttgttggag aggagcttc acagtgcgc ttccgatgt 
ctcctagac aatacttcc ggttttttg cgtttagat 
ataagaggg cgtgttaag gtactccac ggccaaaat 
cgtaagtca gatgaggca cctttggcc ctttttgcc 
atttgtacc ccttttgga ccaccctcc cttctggag 
ataccgcag aaggaagtc atgtttaag agtaagcac 
aagttggac ttggccttg gtttcgatg gatcagaga 
ggaaaaatg ttactgggg tattctgat tcggagttg 
gccacgatg ggagggtac gacctagtg cactacgat 
gatttggcc tacgtggca agtgcacac caggaatta 
ctcaaaact ggcgcttct ggcatgata gcgtaccgt 
ttccaaaca aaagacggt caatggcag tggctccaa 
accagttct cgtctagtc tacaaaaac tccaaaccg 
gattttgtg attagcacc caccggcct ttaatggag 
gaagaaggt cgcgacctc ctcggcaaa cgtacaatg 
gatttcaaa gtgagttac ttagacgcc ggtcttcca 
aacagctac ttctccgaa agcgacctc ctctccacc 
cccactgct caacaacac aacctgacc ccatctcgc 
gtcaaccgt cgctacaag acccaactt cgcgatttt 
ttatcaaca tgtcgcaca aaacgcaaa ctatcaaac 
tccggtcaa gtaactcct cccactaac acaacagtc 
accgcctcc gtgtccagc cccctcccc gactacatc 
gcccctgac tacaacatg tacagcacc tcctatccc 
acgcccacg cccgacctc tcctacatg ccccactcc 
aacttctac ccgaccacg ccccttgac aaccggtac 
ttgaccacg cccgacaac ctcttccac caataccgc 
cctctcggc acctactac cccgactat cactccacc 
ccctcctac aacggcttt ccctacgag cccaccccc 
accaactac ccctgccag cagtcctac cccaactcc 
tggccctac tcggtgagc ccttcgtca gggatcatc 
caaccagtg caagtcatg gagttgggg aagccccca 
aaaatccca aaaatcgag gcccccaaa atggaggag 
cgggagtat gtgggggag gcgccccgc cagacggtg 
ctcatgtgg gggtcgaac ccccccacc accagtgac 
gaaagcagc gagctgacc aatcagtgg aacggcgag 
gagaagact gagcagaac gaggacagt catccccac 
cacaacccc caccatcat cacaattcg cgcgttgtc 
atggtgttg tga
    mRNA sequence
    • >TC011105 RA
      cttggtata aatgcacaa tggaattat gtgtcctga 
agccaacgc ccaaagacg gcgtgacga agagcaacc 
ctagcaaac gtcaccgtg agcgtctca acgccgaat 
tggacacat tggccagtt tgttgcctt ttgagcaaa 
atatcctct cgaaattgg accgactgt cgatcctgc 
gactctccg tcagctatc tacgcacca aaagctact 
ttcaagttg ttatgcaca aagataagg acgatggcg 
gcctacatc ggacccgag atctcaccg ccttcgacc 
acactcccc tagatggcg aaatgttcc tccaggcct 
tgaatgggt tcctcatga ttctgacgt gcgaaggtg 
aagtttttt tcgcaacgc acagcattg aagggtact 
tagggttcc accagtctg atattgtcc accaatccg 
tctatgaac tcgtccatt cagaagata gagaagaat 
tacaaagac agttaatgt ggaactcgt ttcttccac 
ccgaatcgg ctaatatgg gccttcaag atgttctcc 
tacctgaaa attgtcact tgttggaga ggagcttca 
cagtgcgct tccgatgtc tcctagaca atacttccg 
gttttttgc gtttagata taagagggc gtgttaagg 
tactccacg gccaaaatc gtaagtcag atgaggcac 
ctttggccc tttttgcca tttgtaccc cttttggac 
caccctccc ttctggaga taccgcaga aggaagtca 
tgtttaaga gtaagcaca agttggact tggccttgg 
tttcgatgg atcagagag gaaaaatgt tactggggt 
attctgatt cggagttgg ccacgatgg gagggtacg 
acctagtgc actacgatg atttggcct acgtggcaa 
gtgcacacc aggaattac tcaaaactg gcgcttctg 
gcatgatag cgtaccgtt tccaaacaa aagacggtc 
aatggcagt ggctccaaa ccagttctc gtctagtct 
acaaaaact ccaaaccgg attttgtga ttagcaccc 
accggcctt taatggagg aagaaggtc gcgacctcc 
tcggcaaac gtacaatgg atttcaaag tgagttact 
tagacgccg gtcttccaa acagctact tctccgaaa 
gcgacctcc tctccaccc ccactgctc aacaacaca 
acctgaccc catctcgcg tcaaccgtc gctacaaga 
cccaacttc gcgattttt tatcaacat gtcgcacaa 
aacgcaaac tatcaaact ccggtcaag taactcctc 
ccactaaca caacagtca ccgcctccg tgtccagcc 
ccctccccg actacatcg cccctgact acaacatgt 
acagcacct cctatccca cgcccacgc ccgacctct 
cctacatgc cccactcca acttctacc cgaccacgc 
cccttgaca accggtact tgaccacgc ccgacaacc 
tcttccacc aataccgcc ctctcggca cctactacc 
ccgactatc actccaccc cctcctaca acggctttc 
cctacgagc ccaccccca ccaactacc cctgccagc 
agtcctacc ccaactcct ggccctact cggtgagcc 
cttcgtcag ggatcatcc aaccagtgc aagtcatgg 
agttgggga agcccccaa aaatcccaa aaatcgagg 
cccccaaaa tggaggagc gggagtatg tgggggagg 
cgccccgcc agacggtgc tcatgtggg ggtcgaacc 
cccccacca ccagtgacg aaagcagcg agctgacca 
atcagtgga acggcgagg agaagactg agcagaacg 
aggacagtc atccccacc acaaccccc accatcatc 
acaattcgc gcgttgtca tggtgttgt gatgaatgt 
ggaggggga ctcgtgtat ataactcca gcacacgta 
ttttcctcc gcgcaaaca tatcttcct gtgtttttg 
ttatattat tgtcaatgt cttcgatta ggacctaac 
ctttctttt tttttggtt gttatttat ttttagttt 
ttacgcagt gaaaacatg ctagtcaac tggttacat 
acttgtata tgttgtaca ttattattt atgaatgta 
gtaatataa tgagtagat tagtccatt gttcgattg 
tttttctat aaatattta caaaacaaa acgaaatat 
ataacttca tgtacggtt gagttttag ggacgagca 
aacaacatt ccattcgag atctttcaa ttgtacatt 
aacgtttaa gaatcttgt attacctgt aacgtgtat 
gaaaattat ataatgata aaataaatt atattacaa 
tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY