Status
Please wait ...
Gene information
General information
TC032400
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002929
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6095491..6103618
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032400
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06940

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: larvae without detectable defects but die during shedding of the skin
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032400 RA
      MVSLPPSLQ KQVSVMSMN LSAKLDELQ RGDRHLETT 
      VALCEIRSQ LQELTKSVE SCQSEVSEV KRDMVAIKH 
      ELDTVQQVK EEIEELREY VDRLEEHTQ RRKLRLLEQ 
      DLTFFLSYA ILAAVLGML QFGYNTGVI NAPEQNIEH 
      FMKDVYKTR YGKDLSDDS ARGLYSIAV SIFALGGML 
      GGFGGGMVA NKFGRKGGL LLNNVLGIT GACLMWCTK 
      LANSYEILF FGRFIIGVN CGLNTSLVP MYISEIAPL 
      NLRGGLGTV NQLAVTVGL LLSQVLGIE QILGTNDGW 
      PLLLGLAVC PALLQLVLL PICPESPRY LLITKQWEE 
      EARKALRRL RASNQVEED IEEMRAEER AQQAEASIS 
      MLELICSPT LRAPLIIGV VMQLSQQLS GINAVFYYS 
      TSLFVSSGL TEESAKFAT IGIGAIMVV MTLVSIPLM 
      DKTGRRTLH LYGLGGMFI FSIFITISF LIKEMIDWM 
      SYLSVVSTL GFVVFFAVG PGSIPWMIT AELFSQGPR 
      PAAMSIAVL VNWIANFLV GIGFQSLKT WKKREWT
    CDS sequence
    • >TC032400 RA
      atggtatcc ttgcctccc agtctgcaa aaacaggtg 
      agcgtgatg agtatgaat ctgagcgcc aaactggac 
      gagctccaa cgtggagat cggcacctg gagacaacg 
      gtcgctctt tgtgagatc aggtcgcag ctgcaggag 
      ctcaccaag tcggtggag tcctgccag tcggaggtg 
      tcggaggtc aagagggac atggtggcc atcaagcac 
      gagctcgac actgtgcag caagtcaag gaggagatc 
      gaggagctg agagagtac gtggaccgg ctcgaggag 
      cacacgcag cgcaggaaa ctgcggctg ctcgagcag 
      gacctcacg tttttccta tcgtatgcg atcctggcg 
      gcggtcctg ggcatgctg cagttcggt tacaacaca 
      ggagtgata aacgcgccg gaacagaac attgaacac 
      ttcatgaag gacgtgtac aagacgaga tatggaaaa 
      gacctatct gatgattct gcacggggc ctctactcc 
      attgccgtg tccatattt gcgttagga ggcatgcta 
      ggtggcttc ggcggcgga atggtggct aacaaattc 
      ggaaggaaa ggcgggctg cttttgaac aacgtcctg 
      ggaataact ggggcgtgc ttaatgtgg tgtaccaaa 
      ctagcgaac tcatatgaa atactattt tttgggaga 
      tttattata ggggtcaat tgtggactg aacacatcc 
      ttagtacct atgtatata tccgaaata gctccttta 
      aatttacgt ggtggtctt gggacagtg aaccaatta 
      gcggttacg gtgggtctt ctgttatca caagtgctc 
      ggtatcgaa caaatcctc ggtaccaac gacggttgg 
      cctctacta cttggctta gccgtgtgt cctgcctta 
      cttcaatta gtacttctt ccaatttgt ccggaaagt 
      ccgcgctac ctgctcatc actaagcaa tgggaggag 
      gaggcgcgg aaggcattg cggaggttg agggcgagc 
      aaccaggtc gaagaagac atcgaagag atgcgggcg 
      gaggaacgc gcacagcag gccgaagct tccatcagc 
      atgctggag ctgatctgc tcgccgacg ctgcgggcg 
      ccgctgatc atcggcgtc gtcatgcag ctctcgcag 
      cagctgtcc ggaatcaac gcggtgttc tactactcg 
      accagtctg ttcgtcagc tcggggcta acggaggag 
      agcgccaag ttcgccacc atcggcatc ggggccatc 
      atggtggtc atgacgctg gtttccatc ccgctgatg 
      gacaagacc ggcagacgg acgctgcat ctttacggc 
      ctcggtggc atgttcatc ttttcgata ttcatcacc 
      atctcattt ttaataaag gagatgata gactggatg 
      tcgtatctg tctgtggtt tcgacgctg ggattcgtt 
      gtgttcttc gccgttggc cccggatcg ataccgtgg 
      atgatcacc gccgaactc ttctcgcaa ggacctcgg 
      ccggctgcc atgtcaatt gcggtttta gtcaactgg 
      attgccaac tttttggtg ggcatcggg ttccaaagt 
      ttaaaaaca tggaaaaag cgtgaatgg acttaa
    mRNA sequence
    • >TC032400 RA
      agtgaaaaa aatgagtct acctaagac ttttgagct 
      ttatgcgaa tttttgtta attgaaatg tctattttg 
      accgcaatt accacaggt atcaaaaaa agctgccat 
      aaatggata agatttgtc aatattacg aatacgtga 
      ctcattcac aactgtttg aagaaaact caatacttt 
      taagaagtt tgacaaaaa attttaaca gtccctttc 
      tttatgtaa atttttact ccaaactcc tactttcac 
      ggaacttga actaaagca ctggaacta ttgtattat 
      ttttgtttc tgttttgtt atttatttc tctgtttat 
      tttaataaa ttttgattt gtggaagat tgtcaaacg 
      ccatcaaga tgccgcgtc gcagtcgaa caccgcgcg 
      tagattagt aactagaag aagtgaagg ggattttcg 
      gagggttaa aggatacct ggggaggtg tccactgta 
      gtttggaac acagccatg gtatccttg cctcccagt 
      ctgcaaaaa caggtgagc gtgatgagt atgaatctg 
      agcgccaaa ctggacgag ctccaacgt ggagatcgg 
      cacctggag acaacggtc gctctttgt gagatcagg 
      tcgcagctg caggagctc accaagtcg gtggagtcc 
      tgccagtcg gaggtgtcg gaggtcaag agggacatg 
      gtggccatc aagcacgag ctcgacact gtgcagcaa 
      gtcaaggag gagatcgag gagctgaga gagtacgtg 
      gaccggctc gaggagcac acgcagcgc aggaaactg 
      cggctgctc gagcaggac ctcacgttt ttcctatcg 
      tatgcgatc ctggcggcg gtcctgggc atgctgcag 
      ttcggttac aacacagga gtgataaac gcgccggaa 
      cagaacatt gaacacttc atgaaggac gtgtacaag 
      acgagatat ggaaaagac ctatctgat gattctgca 
      cggggcctc tactccatt gccgtgtcc atatttgcg 
      ttaggaggc atgctaggt ggcttcggc ggcggaatg 
      gtggctaac aaattcgga aggaaaggc gggctgctt 
      ttgaacaac gtcctggga ataactggg gcgtgctta 
      atgtggtgt accaaacta gcgaactca tatgaaata 
      ctatttttt gggagattt attataggg gtcaattgt 
      ggactgaac acatcctta gtacctatg tatatatcc 
      gaaatagct cctttaaat ttacgtggt ggtcttggg 
      acagtgaac caattagcg gttacggtg ggtcttctg 
      ttatcacaa gtgctcggt atcgaacaa atcctcggt 
      accaacgac ggttggcct ctactactt ggcttagcc 
      gtgtgtcct gccttactt caattagta cttcttcca 
      atttgtccg gaaagtccg cgctacctg ctcatcact 
      aagcaatgg gaggaggag gcgcggaag gcattgcgg 
      aggttgagg gcgagcaac caggtcgaa gaagacatc 
      gaagagatg cgggcggag gaacgcgca cagcaggcc 
      gaagcttcc atcagcatg ctggagctg atctgctcg 
      ccgacgctg cgggcgccg ctgatcatc ggcgtcgtc 
      atgcagctc tcgcagcag ctgtccgga atcaacgcg 
      gtgttctac tactcgacc agtctgttc gtcagctcg 
      gggctaacg gaggagagc gccaagttc gccaccatc 
      ggcatcggg gccatcatg gtggtcatg acgctggtt 
      tccatcccg ctgatggac aagaccggc agacggacg 
      ctgcatctt tacggcctc ggtggcatg ttcatcttt 
      tcgatattc atcaccatc tcattttta ataaaggag 
      atgatagac tggatgtcg tatctgtct gtggtttcg 
      acgctggga ttcgttgtg ttcttcgcc gttggcccc 
      ggatcgata ccgtggatg atcaccgcc gaactcttc 
      tcgcaagga cctcggccg gctgccatg tcaattgcg 
      gttttagtc aactggatt gccaacttt ttggtgggc 
      atcgggttc caaagttta aaaacatgg aaaaagcgt 
      gaatggact taatttaag attatttag ctttttcat 
      acttgtttt agttagaaa atcaacaca gaattactt 
      ttattttac gattctagt tgaaaaaat tctcaaact 
      agtatgaaa aagttaaat cattcttac aattgatta 
      caattttat ggtgtagag gtagataca attcatata 
      cttaggtaa cacagtaat tgcctaatg cggatcaaa 
      tgtcttatt catctttaa attcctctt cttgtcttc 
      catcaaacg taatcgatc gccgctctg gaccattat 
      ttacttggc gtttgattc tgtttttct tctctgagt 
      cgttttcgt ttgttagtg tatcgtctt cgtttatgc 
      tcatattta cactactgt cactcatgt ttaaattta 
      attacttat taggtttta taaaattta ttcgtaatt 
      tttcactgt tgaagattt gctatgtcg tacaaagtg 
      tcaatgagt actcgtagt tctcacgtt aaaaattaa 
      atctatcac gttatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY