General information | |||
TC032400
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 6095491..6103618 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC032400 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06940 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: larvae without detectable defects but die during shedding of the skin |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032400 RA
MVSLPPSLQ KQVSVMSMN LSAKLDELQ RGDRHLETT VALCEIRSQ LQELTKSVE SCQSEVSEV KRDMVAIKH ELDTVQQVK EEIEELREY VDRLEEHTQ RRKLRLLEQ DLTFFLSYA ILAAVLGML QFGYNTGVI NAPEQNIEH FMKDVYKTR YGKDLSDDS ARGLYSIAV SIFALGGML GGFGGGMVA NKFGRKGGL LLNNVLGIT GACLMWCTK LANSYEILF FGRFIIGVN CGLNTSLVP MYISEIAPL NLRGGLGTV NQLAVTVGL LLSQVLGIE QILGTNDGW PLLLGLAVC PALLQLVLL PICPESPRY LLITKQWEE EARKALRRL RASNQVEED IEEMRAEER AQQAEASIS MLELICSPT LRAPLIIGV VMQLSQQLS GINAVFYYS TSLFVSSGL TEESAKFAT IGIGAIMVV MTLVSIPLM DKTGRRTLH LYGLGGMFI FSIFITISF LIKEMIDWM SYLSVVSTL GFVVFFAVG PGSIPWMIT AELFSQGPR PAAMSIAVL VNWIANFLV GIGFQSLKT WKKREWT
- >TC032400 RA
atggtatcc ttgcctccc agtctgcaa aaacaggtg agcgtgatg agtatgaat ctgagcgcc aaactggac gagctccaa cgtggagat cggcacctg gagacaacg gtcgctctt tgtgagatc aggtcgcag ctgcaggag ctcaccaag tcggtggag tcctgccag tcggaggtg tcggaggtc aagagggac atggtggcc atcaagcac gagctcgac actgtgcag caagtcaag gaggagatc gaggagctg agagagtac gtggaccgg ctcgaggag cacacgcag cgcaggaaa ctgcggctg ctcgagcag gacctcacg tttttccta tcgtatgcg atcctggcg gcggtcctg ggcatgctg cagttcggt tacaacaca ggagtgata aacgcgccg gaacagaac attgaacac ttcatgaag gacgtgtac aagacgaga tatggaaaa gacctatct gatgattct gcacggggc ctctactcc attgccgtg tccatattt gcgttagga ggcatgcta ggtggcttc ggcggcgga atggtggct aacaaattc ggaaggaaa ggcgggctg cttttgaac aacgtcctg ggaataact ggggcgtgc ttaatgtgg tgtaccaaa ctagcgaac tcatatgaa atactattt tttgggaga tttattata ggggtcaat tgtggactg aacacatcc ttagtacct atgtatata tccgaaata gctccttta aatttacgt ggtggtctt gggacagtg aaccaatta gcggttacg gtgggtctt ctgttatca caagtgctc ggtatcgaa caaatcctc ggtaccaac gacggttgg cctctacta cttggctta gccgtgtgt cctgcctta cttcaatta gtacttctt ccaatttgt ccggaaagt ccgcgctac ctgctcatc actaagcaa tgggaggag gaggcgcgg aaggcattg cggaggttg agggcgagc aaccaggtc gaagaagac atcgaagag atgcgggcg gaggaacgc gcacagcag gccgaagct tccatcagc atgctggag ctgatctgc tcgccgacg ctgcgggcg ccgctgatc atcggcgtc gtcatgcag ctctcgcag cagctgtcc ggaatcaac gcggtgttc tactactcg accagtctg ttcgtcagc tcggggcta acggaggag agcgccaag ttcgccacc atcggcatc ggggccatc atggtggtc atgacgctg gtttccatc ccgctgatg gacaagacc ggcagacgg acgctgcat ctttacggc ctcggtggc atgttcatc ttttcgata ttcatcacc atctcattt ttaataaag gagatgata gactggatg tcgtatctg tctgtggtt tcgacgctg ggattcgtt gtgttcttc gccgttggc cccggatcg ataccgtgg atgatcacc gccgaactc ttctcgcaa ggacctcgg ccggctgcc atgtcaatt gcggtttta gtcaactgg attgccaac tttttggtg ggcatcggg ttccaaagt ttaaaaaca tggaaaaag cgtgaatgg acttaa
- >TC032400 RA
agtgaaaaa aatgagtct acctaagac ttttgagct ttatgcgaa tttttgtta attgaaatg tctattttg accgcaatt accacaggt atcaaaaaa agctgccat aaatggata agatttgtc aatattacg aatacgtga ctcattcac aactgtttg aagaaaact caatacttt taagaagtt tgacaaaaa attttaaca gtccctttc tttatgtaa atttttact ccaaactcc tactttcac ggaacttga actaaagca ctggaacta ttgtattat ttttgtttc tgttttgtt atttatttc tctgtttat tttaataaa ttttgattt gtggaagat tgtcaaacg ccatcaaga tgccgcgtc gcagtcgaa caccgcgcg tagattagt aactagaag aagtgaagg ggattttcg gagggttaa aggatacct ggggaggtg tccactgta gtttggaac acagccatg gtatccttg cctcccagt ctgcaaaaa caggtgagc gtgatgagt atgaatctg agcgccaaa ctggacgag ctccaacgt ggagatcgg cacctggag acaacggtc gctctttgt gagatcagg tcgcagctg caggagctc accaagtcg gtggagtcc tgccagtcg gaggtgtcg gaggtcaag agggacatg gtggccatc aagcacgag ctcgacact gtgcagcaa gtcaaggag gagatcgag gagctgaga gagtacgtg gaccggctc gaggagcac acgcagcgc aggaaactg cggctgctc gagcaggac ctcacgttt ttcctatcg tatgcgatc ctggcggcg gtcctgggc atgctgcag ttcggttac aacacagga gtgataaac gcgccggaa cagaacatt gaacacttc atgaaggac gtgtacaag acgagatat ggaaaagac ctatctgat gattctgca cggggcctc tactccatt gccgtgtcc atatttgcg ttaggaggc atgctaggt ggcttcggc ggcggaatg gtggctaac aaattcgga aggaaaggc gggctgctt ttgaacaac gtcctggga ataactggg gcgtgctta atgtggtgt accaaacta gcgaactca tatgaaata ctatttttt gggagattt attataggg gtcaattgt ggactgaac acatcctta gtacctatg tatatatcc gaaatagct cctttaaat ttacgtggt ggtcttggg acagtgaac caattagcg gttacggtg ggtcttctg ttatcacaa gtgctcggt atcgaacaa atcctcggt accaacgac ggttggcct ctactactt ggcttagcc gtgtgtcct gccttactt caattagta cttcttcca atttgtccg gaaagtccg cgctacctg ctcatcact aagcaatgg gaggaggag gcgcggaag gcattgcgg aggttgagg gcgagcaac caggtcgaa gaagacatc gaagagatg cgggcggag gaacgcgca cagcaggcc gaagcttcc atcagcatg ctggagctg atctgctcg ccgacgctg cgggcgccg ctgatcatc ggcgtcgtc atgcagctc tcgcagcag ctgtccgga atcaacgcg gtgttctac tactcgacc agtctgttc gtcagctcg gggctaacg gaggagagc gccaagttc gccaccatc ggcatcggg gccatcatg gtggtcatg acgctggtt tccatcccg ctgatggac aagaccggc agacggacg ctgcatctt tacggcctc ggtggcatg ttcatcttt tcgatattc atcaccatc tcattttta ataaaggag atgatagac tggatgtcg tatctgtct gtggtttcg acgctggga ttcgttgtg ttcttcgcc gttggcccc ggatcgata ccgtggatg atcaccgcc gaactcttc tcgcaagga cctcggccg gctgccatg tcaattgcg gttttagtc aactggatt gccaacttt ttggtgggc atcgggttc caaagttta aaaacatgg aaaaagcgt gaatggact taatttaag attatttag ctttttcat acttgtttt agttagaaa atcaacaca gaattactt ttattttac gattctagt tgaaaaaat tctcaaact agtatgaaa aagttaaat cattcttac aattgatta caattttat ggtgtagag gtagataca attcatata cttaggtaa cacagtaat tgcctaatg cggatcaaa tgtcttatt catctttaa attcctctt cttgtcttc catcaaacg taatcgatc gccgctctg gaccattat ttacttggc gtttgattc tgtttttct tctctgagt cgttttcgt ttgttagtg tatcgtctt cgtttatgc tcatattta cactactgt cactcatgt ttaaattta attacttat taggtttta taaaattta ttcgtaatt tttcactgt tgaagattt gctatgtcg tacaaagtg tcaatgagt actcgtagt tctcacgtt aaaaattaa atctatcac gttatc
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
