Status
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Gene information
General information
TC003492 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5931420..5939097
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003492
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003492 RA
      MSNKRKMDE SEDPSVLFL KSTRFWIQR VFVPIVVCI 
GVGGNTVTV MVLTRKRMR CSTNIYLTA LAVADIIYL 
LFVFVLSFS VYDNINDRK YELFWRFFG LTHWLCDAA 
SNASVWLTV SFTLERYIA VCHPIKGKV LCTENRAKI 
IITGIAIFC ILSTLSTTF EYELAMKEK CVRNCTQEE 
LKNRQHVPG KCFQNVTFH HLPNHNIVV SNLQSHESD 
DSNEKLIRE IYTSLEPAI NKLLPVNCT SHPHIIYVP 
VQMFNKTTP KKINTNLID GSVRNNSEV SKDRTDHAH 
LVPTGKLIT NDTSDNDTC CLKKFTVST VLTNLGKNK 
NYKIFISWF PALCFALIP LILIATFNC FLVHAVYKS 
QSRRRKMTN SQETVSMSN ENRITVMLI GVVFLFLIC 
QTPTASFLI YSNFYEAED LATENIHRA LGNIFNFLV 
NINAATNFI MYCILSKKY RTTFRTLFC GRRKNRQDT 
LILTDTRTR YSSFNNGLR RNVSEYHTP RNLEKGKAL 
EQTHYSAL
    CDS sequence
    • >TC003492 RA
      atgagcaat aagcgtaaa atggatgaa agcgaggac 
ccctctgtg ctgttccta aaatccacc agattttgg 
attcagcgg gtgtttgtg cccattgtg gtctgcata 
ggagttggg ggaaacacc gtgaccgtc atggtgctc 
acaaggaaa cgtatgagg tgctcgacg aatatctac 
cttactgct cttgcagtt gctgacatt atttattta 
ttattcgtc ttcgtgctg tcattctcg gtctatgac 
aacatcaac gaccgaaag tatgaactt ttttggagg 
tttttcgga ttgactcac tggctgtgt gacgctgca 
agtaacgca tcagtatgg ttgacggta tctttcact 
ttggaacgg tacatagca gtttgccat ccaattaag 
ggtaaagtg ctgtgcacc gagaaccgc gcaaaaatc 
atcataaca ggaatcgcg atcttctgc attctgtcc 
acgctgtcg acgacgttc gagtacgag ctggcgatg 
aaggaaaag tgcgtccgc aactgcacg caggaagag 
ctgaagaac aggcaacac gtccccggt aaatgcttc 
caaaatgtc accttccat catctgccg aatcataac 
atagtcgtg agcaacctc cagagccac gaaagtgat 
gattcgaac gaaaagcta attagagag atctacact 
agtttagaa ccagccatc aacaaactg ctccctgtc 
aattgcacg tctcatcct catatcatc tacgtccca 
gtccaaatg tttaacaaa accactccc aaaaaaatt 
aatactaat cttatagac ggaagcgtt cgtaataac 
agtgaagtg tcaaaggac cggactgac catgcccat 
ttagtgccc acgggtaaa ttgataaca aatgacact 
tccgacaat gacacctgt tgtttgaag aaattcacg 
gttagcacg gtgctgacc aatctaggc aagaacaag 
aattacaag atttttatt tcgtggttt cctgcattg 
tgcttcgct cttattccc ttgattctc atcgccact 
ttcaactgc tttctggta cacgctgta tacaagtct 
cagagccgc aggaggaaa atgaccaac tcacaggaa 
accgtttcg atgtcgaat gaaaaccgc ataacggtc 
atgttaatt ggcgtagtg tttctattt ttaatctgc 
caaacgccg actgcttcc ttcctgatc tacagcaac 
ttctacgaa gcggaagac ttggccacg gagaacatc 
caccgtgct ttaggcaac atttttaac tttctcgta 
aacataaat gcggccacg aatttcatt atgtactgc 
attttgtcg aaaaagtac agaaccaca tttagaacc 
ttgttctgt ggccgcaga aaaaacaga caagacacg 
ttaatctta acagacact agaacgaga tactcttct 
tttaacaat ggtttgaga aggaacgtt tccgaatat 
cacactcca agaaatttg gagaaaggt aaggccctc 
gagcaaacc cattattcg gctttgtga
    mRNA sequence
    • >TC003492 RA
      gtgtctcgg aacttgtga gcttatgac tccctaagc 
tgtgatagt ggccgtaac aatgacacc tcgtcaatt 
gtgactcga acgtgccca gtgtttgac ttgatttaa 
actgtatct attcaaaat gtgcgctgc ggaagttcc 
agtgaaaaa aatgtgaag aatcagata agagcgatg 
agcaataag cgtaaaatg gatgaaagc gaggacccc 
tctgtgctg ttcctaaaa tccaccaga ttttggatt 
cagcgggtg tttgtgccc attgtggtc tgcatagga 
gttggggga aacaccgtg accgtcatg gtgctcaca 
aggaaacgt atgaggtgc tcgacgaat atctacctt 
actgctctt gcagttgct gacattatt tatttatta 
ttcgtcttc gtgctgtca ttctcggtc tatgacaac 
atcaacgac cgaaagtat gaacttttt tggaggttt 
ttcggattg actcactgg ctgtgtgac gctgcaagt 
aacgcatca gtatggttg acggtatct ttcactttg 
gaacggtac atagcagtt tgccatcca attaagggt 
aaagtgctg tgcaccgag aaccgcgca aaaatcatc 
ataacagga atcgcgatc ttctgcatt ctgtccacg 
ctgtcgacg acgttcgag tacgagctg gcgatgaag 
gaaaagtgc gtccgcaac tgcacgcag gaagagctg 
aagaacagg caacacgtc cccggtaaa tgcttccaa 
aatgtcacc ttccatcat ctgccgaat cataacata 
gtcgtgagc aacctccag agccacgaa agtgatgat 
tcgaacgaa aagctaatt agagagatc tacactagt 
ttagaacca gccatcaac aaactgctc cctgtcaat 
tgcacgtct catcctcat atcatctac gtcccagtc 
caaatgttt aacaaaacc actcccaaa aaaattaat 
actaatctt atagacgga agcgttcgt aataacagt 
gaagtgtca aaggaccgg actgaccat gcccattta 
gtgcccacg ggtaaattg ataacaaat gacacttcc 
gacaatgac acctgttgt ttgaagaaa ttcacggtt 
agcacggtg ctgaccaat ctaggcaag aacaagaat 
tacaagatt tttatttcg tggtttcct gcattgtgc 
ttcgctctt attcccttg attctcatc gccactttc 
aactgcttt ctggtacac gctgtatac aagtctcag 
agccgcagg aggaaaatg accaactca caggaaacc 
gtttcgatg tcgaatgaa aaccgcata acggtcatg 
ttaattggc gtagtgttt ctattttta atctgccaa 
acgccgact gcttccttc ctgatctac agcaacttc 
tacgaagcg gaagacttg gccacggag aacatccac 
cgtgcttta ggcaacatt tttaacttt ctcgtaaac 
ataaatgcg gccacgaat ttcattatg tactgcatt 
ttgtcgaaa aagtacaga accacattt agaaccttg 
ttctgtggc cgcagaaaa aacagacaa gacacgtta 
atcttaaca gacactaga acgagatac tcttctttt 
aacaatggt ttgagaagg aacgtttcc gaatatcac 
actccaaga aatttggag aaaggtaag gccctcgag 
caaacccat tattcggct ttgtgatgc tcaaacgga 
ccgtttgtg tgctaataa tttgtgctt ataagt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY