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Gene information
General information
TC003484 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5861216..5865561
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003484
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06930

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    1

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003484 RA
      MLEDDVSFT KPVGSYGFS RDEVFALIE RVQNGEQKL 
      TKEEFKTLL KNQHSLRYF KYNIDQYCK LHSLVLEEI 
      STSAEYITF LKDDHTFQK AIRDSDQFY VSFFQTVAP 
      ILIDVCIKF EEAEVFTET AKLFQRCVF SINVKELNS 
      YLTQLFEQT KSEEKHSFS KAVLKFLKN LYTKNATCA 
      TGFKLIFDS FIYSQRNFR KVFEMLLVL LEIIGFDTR 
      SEFNFNKVK YHVENPSLS NSLNILHEL LVVVTSTKT 
      NLECNIGNV TFNDFIKNL LKSIMALTK VPNKCCYQI 
      FTAVIEINP LIIESLISE TLIYIMLSN SSQCRQEYE 
      ELMIKILEV FSKLHRIQN LISKMIPTL KSGLEDRKL 
      AEGVYTFKG ELDCQSDEN ISFNIDTIL PKSVLNYFT 
      NCITNLASW QVINLFKTL LFHFNKTVE ELPEKSEET 
      IYTEILSCF LCWVLMSVR MAEHTVAPA TVVKFIGGL 
      EELKNVLGN LGRALLQRE HNQILMRAF LNISYYWAE 
      IYMSLEYYS VSNDFKLIK VIDSNFSAC NLTYLHSYL 
      SDKEWCLIS ERINNFGEK PCKQIMQKL VIQKLRAVV 
      MSHDSNSEI TSAIIWYLS SDFDDSFAE IFTDRFVVN 
      HLLGQMPPT MIVSLAEII FKDLLEHNG EIFNQPHVS 
      DSRLITEGI LCVIFTKIN KLLKQKRKL DDTTLRKTL 
      YSKVLSLLP TDLFLAPNI GESLGKASQ IVSEGNEQV 
      IDLKLHSDK IQAYLDAFR KLPVLYISE DTQKFIILL 
      LFSLLKDIN HSNCEEEIK NSCESLIIS IIQHNKFTL 
      LDIFEADVL LKLLIENVA RCEDLFAHI VQSAFKCDS 
      TIAKFESGI AFIKDNLKS EQCLRFAVN VLSVLQKMK 
      KVKLTPESK ELFLKYKAD VCRKIVKLV LKKSPDDRL 
      IEPFVLVLK SFLAKDEDD NLSKLKISL SDYVDYCLG 
      NLSDANLKG SLLLFSTIL FNKSLLANL DGDFIMHTY 
      NSLKRVTVK TDILEEYSQ LMILIASLI PNDQFSFIV 
      ADLLQVTTK AVESRENQE ICKHLKTWT CILSTNFNP 
      VKTNILREQ LESLLEKLV LMLKSVDYN EELFEEVIQ 
      FEVSIIQAN HVPLSAAII DFSLLTVSI LMMKRDHDF 
      VHCFNCGVA LLEHLLKFR NPLIMDRLP PYLLQYRVL 
      LKGLCLKSS SRVMVESSD GQKIADSAH KLEKLTKSL 
      TDCTRDMAR IAMFLIADI LAQYEMDTL YPNVKKHLD 
      NCIYSLIAL CDQHAVQYL MRVLSNAST EIFKFMYEN 
      YKKYYRFTG KV
    CDS sequence
    • >TC003484 RA
      atgttggaa gacgacgta tcgtttacc aaaccagta 
      ggtagttat ggtttctcc agagacgag gtttttgca 
      ctcatagaa agagtacaa aacggcgaa cagaaactt 
      acaaaagag gaattcaaa acgttatta aaaaatcaa 
      cactctctt agatacttt aagtacaat attgatcag 
      tattgcaag ttgcacagc cttgttctt gaagaaata 
      agcaccagc gctgagtac ataactttc ctaaaagat 
      gatcacact tttcaaaaa gcgatacgt gacagtgac 
      caattttac gttagtttt ttccaaacg gtggctcca 
      atattgatt gatgtttgc atcaagttt gaggaagct 
      gaagtgttt acagagaca gcaaagttg ttccaaagg 
      tgtgttttt agcataaat gttaaagaa cttaactcg 
      tatttaaca caactgttt gaacaaaca aaatctgaa 
      gagaaacac agtttttct aaagcagtg ttgaagttt 
      cttaaaaat ttgtatacc aaaaatgcc acttgtgct 
      actggtttc aaattaatt tttgattcg tttatttat 
      tcacaaaga aattttcga aaagtgttt gaaatgttg 
      ctagttttg cttgaaata attgggttc gatacaaga 
      agtgaattt aactttaac aaagttaaa taccacgta 
      gaaaatcct tccttgagt aattctttg aatatttta 
      cacgagctt ttggttgtt gtgaccagt acaaaaact 
      aatttagaa tgtaacatt ggtaatgtt actttcaat 
      gattttatt aaaaacctt ttaaaatca atcatggcc 
      ttaaccaaa gttccgaat aagtgttgt tatcagatt 
      tttaccgca gttattgaa atcaaccct ttgataatc 
      gagtcactt atcagcgaa acgcttatc tatataatg 
      ttaagtaac agcagtcaa tgccgacaa gaatatgag 
      gaattgatg attaaaatc cttgaagtg ttcagcaaa 
      ttacaccgg attcaaaat ctgatctct aaaatgatt 
      ccgacatta aaatcgggt cttgaggac cggaaatta 
      gccgaaggc gtttacaca tttaaagga gagttagat 
      tgccaatca gatgaaaac atttcgttc aacattgac 
      actattctc cccaaatca gttctaaat tatttcacc 
      aactgtatc acaaatttg gccagttgg caggtgata 
      aatttgttc aaaacactc ttgttccac ttcaataaa 
      actgtggag gaactgccg gaaaaatca gaagaaacc 
      atttacacc gaaattttg agttgcttt ctctgttgg 
      gttttgatg agtgtgcga atggcggag cacactgtg 
      gccccagcc accgttgtc aaattcata gggggcctg 
      gaggagctc aaaaacgtg ttagggaac ttgggtagg 
      gctttattg caacgagaa cacaatcaa attctaatg 
      cgcgctttt ttgaacatt tcgtactat tgggccgaa 
      atttacatg tccctggaa tattattcg gtcagtaac 
      gatttcaaa ttgattaaa gtcattgac tcgaatttt 
      tcggcgtgt aatttgacc tatctacac tcgtatttg 
      agcgacaaa gagtggtgc cttatttcc gaaaggatc 
      aataatttc ggagaaaag ccgtgcaag caaataatg 
      caaaaactt gtgattcag aagttgcga gctgttgta 
      atgtcgcat gatagtaac agtgagatt acgagcgcg 
      attatttgg tatctctca tcggatttt gacgatagt 
      ttcgctgaa attttcacg gacagattc gtggtcaat 
      catttactt ggccaaatg cccccgacg atgattgtc 
      agtttggct gaaattatt tttaaagat ttactagaa 
      cataacggg gaaatattt aaccaacct cacgtctcc 
      gactctcgg ctcataaca gaaggcatt ctttgcgtc 
      atttttaca aaaattaac aaattgctc aaacaaaaa 
      cgaaaactt gacgatact actctacgg aaaacgctc 
      tattcaaaa gtattgtct ctcctaccc acggacttg 
      ttcttggca ccgaatatt ggagaatcg ttagggaag 
      gcctcacaa attgtctct gaaggcaac gaacaagtc 
      atagattta aaactacac agcgacaag attcaggcg 
      tatttagac gcatttaga aaactccca gtactgtat 
      ataagtgag gacacgcaa aaatttata atcctgcta 
      ttattctcg ttactcaaa gatataaat cacagtaat 
      tgcgaggaa gaaataaag aacagttgt gaaagtctc 
      atcattagc attatacaa cataataag tttacttta 
      ttggatatt ttcgaagcg gatgtcctt ctaaaactc 
      ctgattgaa aatgtcgca agatgtgaa gatctcttt 
      gcccacatc gtccaaagt gccttcaag tgtgactcg 
      acgattgcc aaatttgaa tcagggatt gcgtttatt 
      aaagacaat cttaaatcg gagcagtgc ctccgattt 
      gcggtaaat gtactgagt gtgcttcag aaaatgaaa 
      aaagttaaa ttaacacca gaatcgaag gaattattt 
      ttgaaatac aaggcggat gtttgccgt aaaatcgtt 
      aaactcgtg ttgaagaaa agcccagat gatcgcctg 
      atcgagccg tttgttctg gttttgaag tcgtttctg 
      gccaaagac gaggacgac aatctctcg aagttgaaa 
      atttcgttg agcgattat gtcgattat tgtcttggg 
      aatttgtca gatgcgaac cttaagggc agtttgttg 
      cttttttcg acaattttg ttcaataaa tcgttgttg 
      gccaatctt gacggtgac tttattatg cacacttac 
      aatagtttg aaacgcgtg actgtaaag actgacatt 
      ttggaggag tattcacag ttaatgatt ctaatcgcg 
      tcgttaatt ccaaatgat caattttcg ttcattgtt 
      gccgatttg ttgcaagtg acgactaaa gcagtcgag 
      tcgagagaa aaccaagag atttgcaaa cacttaaaa 
      acatggact tgcatcctt tctacaaat tttaatcct 
      gttaaaacc aacattttg cgtgaacag ctcgaatcc 
      ctcttggag aaattagtt ctcatgttg aagtctgta 
      gactacaac gaggaatta tttgaagaa gttatccaa 
      tttgaggtt tcaattatt caagcaaat cacgtgccg 
      ctaagtgct gccattata gacttttcc ctgttaacg 
      gtttcgatt ttaatgatg aagcgggac cacgatttt 
      gtgcactgt ttcaactgt ggagtcgcc ctattagaa 
      catttgcta aaattccga aacccctta attatggat 
      cgcttgcct ccctatttg ttgcagtac agagtgttg 
      cttaaaggg ctgtgtctg aaaagtagt tccagagtg 
      atggtggag tctagtgac gggcagaaa attgccgat 
      tctgctcat aaattagag aaattgaca aaaagtctc 
      actgattgt actagagat atggccagg attgcaatg 
      tttctgatt gcggatatt ttggcacag tatgaaatg 
      gacactctg taccccaac gtcaagaaa catttggat 
      aactgcata tacagtctc atcgctttg tgtgatcag 
      catgcagtt cagtatttg atgagggtt ttaagcaac 
      gcgtctact gaaatattt aaatttatg tatgagaat 
      tataaaaaa tattacaga tttacagga aaggtttaa
    mRNA sequence
    • >TC003484 RA
      tgcaagttt caaatttat ttacaacca aaaacttta 
      taaatttat taattatac aaatgtttt aagttagca 
      aaatgttgg aagacgacg tatcgttta ccaaaccag 
      taggtagtt atggtttct ccagagacg aggtttttg 
      cactcatag aaagagtac aaaacggcg aacagaaac 
      ttacaaaag aggaattca aaacgttat taaaaaatc 
      aacactctc ttagatact ttaagtaca atattgatc 
      agtattgca agttgcaca gccttgttc ttgaagaaa 
      taagcacca gcgctgagt acataactt tcctaaaag 
      atgatcaca cttttcaaa aagcgatac gtgacagtg 
      accaatttt acgttagtt ttttccaaa cggtggctc 
      caatattga ttgatgttt gcatcaagt ttgaggaag 
      ctgaagtgt ttacagaga cagcaaagt tgttccaaa 
      ggtgtgttt ttagcataa atgttaaag aacttaact 
      cgtatttaa cacaactgt ttgaacaaa caaaatctg 
      aagagaaac acagttttt ctaaagcag tgttgaagt 
      ttcttaaaa atttgtata ccaaaaatg ccacttgtg 
      ctactggtt tcaaattaa tttttgatt cgtttattt 
      attcacaaa gaaattttc gaaaagtgt ttgaaatgt 
      tgctagttt tgcttgaaa taattgggt tcgatacaa 
      gaagtgaat ttaacttta acaaagtta aataccacg 
      tagaaaatc cttccttga gtaattctt tgaatattt 
      tacacgagc ttttggttg ttgtgacca gtacaaaaa 
      ctaatttag aatgtaaca ttggtaatg ttactttca 
      atgatttta ttaaaaacc ttttaaaat caatcatgg 
      ccttaacca aagttccga ataagtgtt gttatcaga 
      tttttaccg cagttattg aaatcaacc ctttgataa 
      tcgagtcac ttatcagcg aaacgctta tctatataa 
      tgttaagta acagcagtc aatgccgac aagaatatg 
      aggaattga tgattaaaa tccttgaag tgttcagca 
      aattacacc ggattcaaa atctgatct ctaaaatga 
      ttccgacat taaaatcgg gtcttgagg accggaaat 
      tagccgaag gcgtttaca catttaaag gagagttag 
      attgccaat cagatgaaa acatttcgt tcaacattg 
      acactattc tccccaaat cagttctaa attatttca 
      ccaactgta tcacaaatt tggccagtt ggcaggtga 
      taaatttgt tcaaaacac tcttgttcc acttcaata 
      aaactgtgg aggaactgc cggaaaaat cagaagaaa 
      ccatttaca ccgaaattt tgagttgct ttctctgtt 
      gggttttga tgagtgtgc gaatggcgg agcacactg 
      tggccccag ccaccgttg tcaaattca tagggggcc 
      tggaggagc tcaaaaacg tgttaggga acttgggta 
      gggctttat tgcaacgag aacacaatc aaattctaa 
      tgcgcgctt ttttgaaca tttcgtact attgggccg 
      aaatttaca tgtccctgg aatattatt cggtcagta 
      acgatttca aattgatta aagtcattg actcgaatt 
      tttcggcgt gtaatttga cctatctac actcgtatt 
      tgagcgaca aagagtggt gccttattt ccgaaagga 
      tcaataatt tcggagaaa agccgtgca agcaaataa 
      tgcaaaaac ttgtgattc agaagttgc gagctgttg 
      taatgtcgc atgatagta acagtgaga ttacgagcg 
      cgattattt ggtatctct catcggatt ttgacgata 
      gtttcgctg aaattttca cggacagat tcgtggtca 
      atcatttac ttggccaaa tgcccccga cgatgattg 
      tcagtttgg ctgaaatta tttttaaag atttactag 
      aacataacg gggaaatat ttaaccaac ctcacgtct 
      ccgactctc ggctcataa cagaaggca ttctttgcg 
      tcattttta caaaaatta acaaattgc tcaaacaaa 
      aacgaaaac ttgacgata ctactctac ggaaaacgc 
      tctattcaa aagtattgt ctctcctac ccacggact 
      tgttcttgg caccgaata ttggagaat cgttaggga 
      aggcctcac aaattgtct ctgaaggca acgaacaag 
      tcatagatt taaaactac acagcgaca agattcagg 
      cgtatttag acgcattta gaaaactcc cagtactgt 
      atataagtg aggacacgc aaaaattta taatcctgc 
      tattattct cgttactca aagatataa atcacagta 
      attgcgagg aagaaataa agaacagtt gtgaaagtc 
      tcatcatta gcattatac aacataata agtttactt 
      tattggata ttttcgaag cggatgtcc ttctaaaac 
      tcctgattg aaaatgtcg caagatgtg aagatctct 
      ttgcccaca tcgtccaaa gtgccttca agtgtgact 
      cgacgattg ccaaatttg aatcaggga ttgcgttta 
      ttaaagaca atcttaaat cggagcagt gcctccgat 
      ttgcggtaa atgtactga gtgtgcttc agaaaatga 
      aaaaagtta aattaacac cagaatcga aggaattat 
      ttttgaaat acaaggcgg atgtttgcc gtaaaatcg 
      ttaaactcg tgttgaaga aaagcccag atgatcgcc 
      tgatcgagc cgtttgttc tggttttga agtcgtttc 
      tggccaaag acgaggacg acaatctct cgaagttga 
      aaatttcgt tgagcgatt atgtcgatt attgtcttg 
      ggaatttgt cagatgcga accttaagg gcagtttgt 
      tgctttttt cgacaattt tgttcaata aatcgttgt 
      tggccaatc ttgacggtg actttatta tgcacactt 
      acaatagtt tgaaacgcg tgactgtaa agactgaca 
      ttttggagg agtattcac agttaatga ttctaatcg 
      cgtcgttaa ttccaaatg atcaatttt cgttcattg 
      ttgccgatt tgttgcaag tgacgacta aagcagtcg 
      agtcgagag aaaaccaag agatttgca aacacttaa 
      aaacatgga cttgcatcc tttctacaa attttaatc 
      ctgttaaaa ccaacattt tgcgtgaac agctcgaat 
      ccctcttgg agaaattag ttctcatgt tgaagtctg 
      tagactaca acgaggaat tatttgaag aagttatcc 
      aatttgagg tttcaatta ttcaagcaa atcacgtgc 
      cgctaagtg ctgccatta tagactttt ccctgttaa 
      cggtttcga ttttaatga tgaagcggg accacgatt 
      ttgtgcact gtttcaact gtggagtcg ccctattag 
      aacatttgc taaaattcc gaaacccct taattatgg 
      atcgcttgc ctccctatt tgttgcagt acagagtgt 
      tgcttaaag ggctgtgtc tgaaaagta gttccagag 
      tgatggtgg agtctagtg acgggcaga aaattgccg 
      attctgctc ataaattag agaaattga caaaaagtc 
      tcactgatt gtactagag atatggcca ggattgcaa 
      tgtttctga ttgcggata ttttggcac agtatgaaa 
      tggacactc tgtacccca acgtcaaga aacatttgg 
      ataactgca tatacagtc tcatcgctt tgtgtgatc 
      agcatgcag ttcagtatt tgatgaggg ttttaagca 
      acgcgtcta ctgaaatat ttaaattta tgtatgaga 
      attataaaa aatattaca gatttacag gaaaggttt 
      aaaatatgt ttacaataa aaaatataa tacgtt
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