Status
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Gene information
General information
TC002973 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5508958..5512838
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002973
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06926

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002973 RA
      MPPPNDELV RKRTSADFV FGKVIGEGS FSTVYLAKD 
      VHTSREFAI KVLEKRHII REKKTEYVT REKKVLQIL 
      GSSCKYFVH LYYTFQDTD RLYFVLTYA KNGELLQQI 
      NKDGAFSLE CTKYYAAEL VLALEYLKL KGIVHRDLK 
      PENVLFDEN WHILIIDFG SAKILGEDK SSDEESQKR 
      RKYSFVGTA QYVSPEILN DDESTCASD LWALGCIIY 
      QMLTGQMPF RGPSEYLIF QKILKLDYE FPQGFDKVA 
      KDLIEKFLV IAPSSRLGA TDQVPYKSI REHEFFREI 
      NWDDLGPPP NFSAKCENS SDIVIIPDD LEPGLDEEK 
      ASRLHFRML QPQTPPRKK SEPNRKITD LSQEEIEQR 
      LEQQKSNNS VYHELVEGN LILKQGIVE KRKGIFPRR 
      RMLLLTLGP HLYYVDPVT MTLKGEIPW SDDLKPEAK 
      NFKKFFVHT PNRIYYLED PDGYALEWC KAIDEVKAY 
      YYPVQQL
    CDS sequence
    • >TC002973 RA
      atgccgccc cccaacgac gagctggtg cggaagcgg 
      acctctgca gactttgtc ttcgggaaa gtcatcggg 
      gagggcagc ttcagcacg gtctacctc gccaaagac 
      gtgcacacg agccgagag ttcgcaatc aaagtcctg 
      gaaaagcgg cacattatc agggagaag aagaccgag 
      tacgtgacc cgcgagaag aaagtcctg caaatttta 
      ggcagctcc tgtaaatat tttgtccac ttgtactac 
      acgtttcaa gacaccgac cggctgtac tttgtgcta 
      acttacgcg aagaacgga gagttgctc cagcagatc 
      aacaaagac ggcgcgttt agcctcgaa tgcaccaag 
      tattacgca gccgagctc gtccttgcc ttggaatac 
      cttaagctc aaaggaatc gtgcacagg gatttgaag 
      ccggagaac gtcctgttc gacgaaaac tggcacatt 
      ttgatcatt gacttcgga agcgccaaa atcctaggc 
      gaggacaag tcctcagac gaggagagt caaaagcga 
      cgtaaatac agttttgtg ggaactgca caatatgtt 
      agtcctgag atactaaac gatgatgaa tcaacttgc 
      gcctccgat ttgtgggcc ttggggtgc atcatctac 
      cagatgcta acgggtcaa atgccgttc cgcggtcct 
      agtgaatat ttaattttc cagaaaatt ttgaaactc 
      gactacgag ttcccccaa ggttttgat aaagtagcg 
      aaagactta atagagaaa ttccttgtt atagccccc 
      agtagtagg ctgggggcg acggatcaa gtaccttac 
      aaaagtata cgagagcat gagttcttt agggaaatt 
      aattgggac gatttgggg ccccctccg aacttttcg 
      gcgaaatgt gagaacagt agcgatata gtgatcatc 
      ccggatgat ctggaacca ggccttgat gaggagaag 
      gcgtcgaga ctacacttt aggatgctt cagccccag 
      acgccccct aggaaaaaa tcggaaccc aataggaaa 
      ataactgac ttgagtcag gaagaaatt gagcaaaga 
      ctggaacaa caaaaaagt aataacagt gtttatcat 
      gagttagtt gaagggaat ttaattcta aaacaaggg 
      attgttgaa aagagaaag ggaattttc ccgagacgg 
      cggatgttg ttgctcacg ctagggcct catttatac 
      tacgtagat cctgttact atgactcta aagggggaa 
      atcccgtgg agtgacgat ttgaaacca gaggccaaa 
      aattttaaa aagtttttt gttcacacg cccaatcgt 
      atctactat ctggaggac ccagacggg tatgccctg 
      gagtggtgc aaagcaata gacgaagtg aaggcttac 
      tactatcca gtacaacaa ctgtag
    mRNA sequence
    • >TC002973 RA
      gaatgtttg ttagtctgc gagtacatg agataaaaa 
      aatcaattc aggacgaaa aatggcaat aagcgatca 
      atgtagtaa aaaatattc ctccttttt agttattta 
      tttttctat atacaacac agcggcttt ttcgatgtt 
      tattaaaca ttcatgtaa tacggcaac gtgagaaag 
      ctttcagca gtgctgaat gaataataa taaatgtat 
      tttcgtctg aaagaaatt ttggtttac aaataatgg 
      ttttttaaa ttgatgtgt acatttttt tctatgcaa 
      attagatat aataaattg gaaaaagca agggagtta 
      tttacactg tttcaactc gctaaggat tttcagtaa 
      aaacacaaa cacatttga aaatcatgc tttattgcc 
      aaatacagc agcgaaaga agtttgccg actagttgt 
      gcggtttta aatagaatc tattgtctt tttatcgat 
      taaatcaaa actttaggg cggttgctt ccgtgcagg 
      attaacctg ataaaaaac tttgcacgt ctcgtgcaa 
      aattttatt ttgttaaaa attgcgaaa cgccggaat 
      taaaaggtg cctttgggg gagatttac ggcttaatc 
      taattttgc atctggtcg tttccaatt tagcacaca 
      aaaaccgga cggatgcga aattcatta agagattag 
      agccaacac ctcagcagg tgaccacga attagggcg 
      gaagcaact ctaagtaga gaattatgt tagcacgat 
      tctggtggc tttacctca aattcaatc gacacacaa 
      atagaatta gtaaaatgt gtcatttct catcgtaac 
      agcgatctc aaaaattat gacttaacg aagttgggg 
      cgctatttg ctgtaacaa acttattgt cataatttg 
      gatgtttct gcaattaaa attaacgag aagggacat 
      ggttggcgc actttccga atccattag cagggagga 
      atttgaaat tattttcta acgaggttg aattgaaat 
      aaacatatt attgtgact ggtaagtaa tttcgaagc 
      atgcgatgt gcagcaagt ggtcaataa aacagaaca 
      aagttctag tcaacatta ctatgtttg ttgctcgta 
      gtaaaaatg gttttctcc gacatgtat gattttaat 
      aacaacgat aacaacagc aataaaaaa ggttgtaaa 
      tacatacca ctcatgcag atatttttg aatgagaat 
      agtcagcaa tgtttgata attttattg aatacgaaa 
      tttttgaag gcctccatt ttagtccgt gtattaaag 
      ctatgaata ttcaactga taaaaaatt aaaaacacc 
      attaatcgt ccacatttg cgacctgcg acgctcggc 
      gtcggtgac tacaaaagc gtctaaagc gcccattta 
      ccatgaacc agacagaca tccaccccc accaccatt 
      tgaaattct ccccactcc cgcgtaaaa tcaatggct 
      tatcctcgg tcggttacg atgatgcct gtttcgttt 
      gttgtttga gtttatctg tcaaaaatc agtgtttat 
      tcggccaaa taaaaaaaa acgcgaatt tcgttgagt 
      tccccttgc gttggttcg tagcagagt gcatttcgg 
      ttcagtttc aagccttga cagcaacga gttcattaa 
      actttccag cgaaatgca cgtcattac cgtattatt 
      attgtccat tgaaacttg gtactgaaa tcacggttg 
      tgacgtatt gtgtgacgt ttttcctga cagtacttt 
      atagctgtt ttattgtcg gacgatgcc gccccccaa 
      cgacgagct ggtgcggaa gcggacctc tgcagactt 
      tgtcttcgg gaaagtcat cggggaggg cagcttcag 
      cacggtcta cctcgccaa agacgtgca cacgagccg 
      agagttcgc aatcaaagt cctggaaaa gcggcacat 
      tatcaggga gaagaagac cgagtacgt gacccgcga 
      gaagaaagt cctgcaaat tttaggcag ctcctgtaa 
      atattttgt ccacttgta ctacacgtt tcaagacac 
      cgaccggct gtactttgt gctaactta cgcgaagaa 
      cggagagtt gctccagca gatcaacaa agacggcgc 
      gtttagcct cgaatgcac caagtatta cgcagccga 
      gctcgtcct tgccttgga ataccttaa gctcaaagg 
      aatcgtgca cagggattt gaagccgga gaacgtcct 
      gttcgacga aaactggca cattttgat cattgactt 
      cggaagcgc caaaatcct aggcgagga caagtcctc 
      agacgagga gagtcaaaa gcgacgtaa atacagttt 
      tgtgggaac tgcacaata tgttagtcc tgagatact 
      aaacgatga tgaatcaac ttgcgcctc cgatttgtg 
      ggccttggg gtgcatcat ctaccagat gctaacggg 
      tcaaatgcc gttccgcgg tcctagtga atatttaat 
      tttccagaa aattttgaa actcgacta cgagttccc 
      ccaaggttt tgataaagt agcgaaaga cttaataga 
      gaaattcct tgttatagc ccccagtag taggctggg 
      ggcgacgga tcaagtacc ttacaaaag tatacgaga 
      gcatgagtt ctttaggga aattaattg ggacgattt 
      ggggccccc tccgaactt ttcggcgaa atgtgagaa 
      cagtagcga tatagtgat catcccgga tgatctgga 
      accaggcct tgatgagga gaaggcgtc gagactaca 
      ctttaggat gcttcagcc ccagacgcc ccctaggaa 
      aaaatcgga acccaatag gaaaataac tgacttgag 
      tcaggaaga aattgagca aagactgga acaacaaaa 
      aagtaataa cagtgttta tcatgagtt agttgaagg 
      gaatttaat tctaaaaca agggattgt tgaaaagag 
      aaagggaat tttcccgag acggcggat gttgttgct 
      cacgctagg gcctcattt atactacgt agatcctgt 
      tactatgac tctaaaggg ggaaatccc gtggagtga 
      cgatttgaa accagaggc caaaaattt taaaaagtt 
      ttttgttca cacgcccaa tcgtatcta ctatctgga 
      ggacccaga cgggtatgc cctggagtg gtgcaaagc 
      aatagacga agtgaaggc ttactacta tccagtaca 
      acaactgta gatgaatgt agaaaactg tttagtttt 
      attgtattt atttatatt ttatttaaa ttatttgtt 
      agcctagca aattattaa tctgtagca gatcgcctt 
      atttataat atattgttt tacttgtac gagagttgt 
      atttttcat cgaaataaa cattaatcc ggccctggt 
      acgttttcg tgtcggcag gttggccgg cctgggtgt 
      caagatggt ggtcagtgg tgtagtttc agtgacata 
      tgaattgtt attcacgat ttccagtgg gaaataaaa 
      ttaatttcg aattaatct caagtggtt ttttgcgta 
      cggacctca atttattta tttgcgata gaatgggac 
      gcttcggcc acaggtaag tttgttgtt attggtttg 
      taattattt tagcattag accacccgc ccactccaa 
      attataaac caccacatg atatttttg caagtattt 
      tcggcggga aataaaccg ttttcctgg tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY