Status
Please wait ...
Gene information
General information
TC002977 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5432673..5475367
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002977
    iBeetle screen iB_06925

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002977 RA
      MSFFNSLQE YVSNSVAGL SLSPKRFSL SRSDTAEPP 
      TGIPPGSRS NSGDSNTPG FPKVLPAPG ASALVQRRR 
      STLECLVPA PPVRRPGSF RQRSPKATP DKRPSFCSR 
      RLSWPDIDH QVRSGVQEI DGSYFESFT ALAWKQENQ 
      RQSVMKAAE VAAADPPPQ EADLGIVAI DYTAAQNEK 
      DKLYVEMLY TIANTVGAP APGGQYAHY KEDLYLYGQ 
      RAFGMPPDR HYRMLHLAG EEKPPIVVL SVVVIEAEG 
      LEAKDANGF SDPYCMLGI QPMAAPSSP QPQPLTPNR 
      TLSDACAPD MQGNHDKLR KHHSFKLSF KRKDGRVRE 
      QRDSIVGAL PAKFIRATS VKPHTLNPK WNEKFRFDI 
      DDINSDSLH LDIWDHDDE SSVLEAVSK LNEVRGVRG 
      LGRFFKQVC QSARQSSQD DFLGCVTIP LQDIPSTGL 
      EGWFKLEAR SQRSSVQGR IRLKMWLST RENRGASEE 
      DNWTELIQH ESLYATFVD YELKNWNRE SWTWSGDLP 
      GPALTILHQ HAVQGDLTE LQTAVARFV AACRVYLKH 
      PLDPRWMLQ LLTDIESAW MNFTLTREE EMWLAEKFT 
      AMQERWLQQ LRHHRQLFP ALHPPSLVR LEYVLRCLA 
      YMSNMKAFW KCCPFNKEI RGEIVATLR KGTPEWFQS 
      LKSSIMTSD EYDACFVDF CSEVYVHLQ HGLSHYHPL 
      FEGTNGIPY FSVVFKQLD KLMSDEVIA FLNQNEHPD 
      AAYSRLIFS VYLEVKDLA TFHQNLPSG GDHKLLLVR 
      CHEWFEASV SCWLIVCKA KALQRVRTA VELQKPCEG 
      DKLVKHSSS AVDVVAMFC QLRDFWRLL QWPKAHSVP 
      LLSQLLDCI CSAALLYAD ISYQGLMET GYFDRMGPF 
      KICDEMCMT VNNLEYVYK FVSLLENYF DFVTLETIA 
      SEIQCATLT NQLDSTLSQ FQVRIMDII KRVGPQMQE 
      ALRKAMFHV AWSPDTLPT NQAVEPLLE YLHTHLHAL 
      NLYLLPQNF ERVLYDVWE YTLSELNHQ MDGGTNTDE 
      MPPMFHERL HAALELMVD FFHCEGNAL SIESLHSSI 
      FCQIEQRLQ YHRTDTETL IEIFYNQRL QEQLNTTSS 
      PYGSLAVRA YFNHDSLCV EVLHARDII PLDPNGFSD 
      PFVIIELLP HRVFPHCNE QQTNVHKKT LHPIFDECF 
      EFSVSLEQC RSPGAMIAF TVMDHDVLT ANDFAGEAF 
      LALGSIPGV ADTTGVDNF HGLKPVELI LMQQHQKNH 
      PILQILESR VADRLAAEF VRKQRQRIA NK
    CDS sequence
    • >TC002977 RA
      atgagcttc ttcaactcc ttacaagag tatgtcagc 
      aatagcgtc gccggactc agcctaagc ccaaaacga 
      ttttcctta agcagaagt gacacggcg gaaccccct 
      acgggaatt cccccggga tcgcgtagc aattcaggt 
      gatagcaac actcccggt tttcccaaa gttctcccg 
      gcacccggt gccagtgct ctcgtccag cgacgccgc 
      agcacttta gaatgcctc gtacctgca cctcctgtc 
      cgaaggccc ggctccttc aggcagcgt tcgccgaaa 
      gccactcct gacaaacgg ccttccttc tgcagcaga 
      aggctgagc tggcccgac atcgatcat caggttcgt 
      tcgggagtt caagaaatt gacggctct tatttcgag 
      agcttcaca gcgctggca tggaaacaa gaaaatcaa 
      cgacagagt gtgatgaaa gcggccgaa gtcgctgct 
      gcggaccct ccgccccag gaagccgat ttgggcatt 
      gtggccatt gactacaca gcggcccaa aatgaaaag 
      gacaagctc tacgtggaa atgctttac accattgcc 
      aacacggtc ggtgctccc gccccagga ggccaatat 
      gcccattac aaagaagac ctctatttg tacggccaa 
      agggcgttc gggatgccg ccagaccgt cactaccgc 
      atgctgcac ctcgcagga gaggaaaaa ccaccgatt 
      gttgttctc agcgtcgtt gtgatagaa gctgaagga 
      ctcgaagcc aaagatgcc aatggtttc agtgacccg 
      tactgcatg ctcggaatc caaccgatg gcagcgccg 
      tcctctcct cagccccag ccgctcacc cctaatcgc 
      accctgtcg gatgcttgc gcccccgac atgcagggc 
      aatcacgac aagctccgg aagcaccac agcttcaaa 
      ctcagcttt aaaagaaaa gacggacga gttagggaa 
      caacgggac agcatcgtc ggtgcactg cccgctaaa 
      ttcattcgc gccacctcg gtcaaaccc cacacgctt 
      aatccgaaa tggaacgag aagttcaga tttgacata 
      gacgacatc aacagcgac tccttgcac ctcgacata 
      tgggaccac gacgacgaa agttcagtg ctagaagca 
      gtcagcaaa ctaaacgag gttcgcgga gttcggggt 
      ttgggccga tttttcaag caagtttgc caatctgcg 
      cgacaaagt tcccaagac gatttcctc ggatgtgtt 
      acgattccc ctccaagac attccatcg acaggcttg 
      gaaggctgg ttcaagctc gaagctcga tctcagcgc 
      agctcggtc cagggccgc atccgcctg aaaatgtgg 
      ctctcgacc cgcgagaac cgcggcgcc tccgaggag 
      gacaactgg accgagctc atccagcac gagagcctc 
      tacgccacg tttgtcgac tacgagcta aaaaactgg 
      aaccgcgag tcctggacc tggagcggg gacctgcca 
      gggccggcg ctcacgatt ctgcaccag cacgcggtc 
      caaggggat ttgacggag cttcaaacc gccgtggcc 
      cgctttgtg gcggcctgc agggtctac ctgaagcat 
      ccgctggat ccccgctgg atgctccag ttactgaca 
      gacattgag agcgcttgg atgaatttc actctgaca 
      agagaggag gaaatgtgg ctcgctgag aagtttacg 
      gcaatgcaa gagagatgg ttgcaacag ttgcgccac 
      caccgacag ttgttccca gccctccat cccccgtct 
      ctggttcga cttgaatac gttctgcga tgccttgcc 
      tacatgtcg aatatgaaa gcattctgg aaatgctgc 
      cccttcaat aaggaaatc cgcggtgag atagtggcc 
      accctccgc aaagggaca cctgaatgg tttcaaagc 
      ctaaaatcg tctattatg acctcggat gaatacgac 
      gcctgtttt gttgacttc tgctcggag gtttatgta 
      catttgcaa cacggacta tctcattat catcctctc 
      tttgagggc acaaacgga atcccctac ttcagcgtg 
      gtgtttaaa cagctagat aagttaatg tcagacgag 
      gtaatcgca tttctgaac caaaacgag cacccggac 
      gcagcttac agccgactt atcttttct gtttacttg 
      gaagtgaaa gatttggcc acttttcat caaaatctg 
      ccgtccggc ggcgatcat aaactgtta ttagtccgt 
      tgtcatgaa tggtttgag gcgtctgtt agttgttgg 
      ttgattgta tgcaaagcg aaggcttta caaagggta 
      cgaactgca gtcgaactt caaaagccc tgcgaaggg 
      gacaaatta gtgaagcac agttcgtct gcagttgat 
      gtagtagcg atgttttgt caattaaga gacttctgg 
      agactcctt caatggcct aaggcgcat tccgttcct 
      cttctctcg caacttctc gactgtata tgttcagca 
      gcgctatta tacgccgat atatcgtat caagggttg 
      atggaaacg gggtatttc gaccggatg gggcccttt 
      aaaatttgc gacgaaatg tgcatgacg gtcaataac 
      ttggaatat gtttacaag tttgtgtct ttattagaa 
      aattacttt gattttgtt acactcgaa acgatcgct 
      tcggaaata caatgcgct actttaact aaccaatta 
      gatagcaca ctaagtcag ttccaagta agaataatg 
      gacataata aagcgtgta gggccacaa atgcaagaa 
      gcacttagg aaagccatg tttcatgtc gcttggtct 
      ccagacact ctacctaca aatcaagcc gtggagccg 
      ctgcttgaa tatctgcac acacatcta catgcactc 
      aacttgtac ttgttgcct cagaacttc gaacgagtc 
      ctatacgac gtctgggag tacacttta tctgaatta 
      aaccatcaa atggatggt ggcacaaac actgacgag 
      atgccccca atgttccac gagagactc cacgcggcg 
      ttggagctc atggttgac ttcttccac tgcgaggga 
      aatgcgctt agtatagaa agcttgcat tcatccata 
      ttctgtcaa atcgaacaa agactgcag tatcacagg 
      acggatacg gaaaccctc atagaaatt ttctacaac 
      caaaggctc caagaacaa ctaaacacg accagcagc 
      ccgtacggg agtctcgca gtccgagcg tacttcaac 
      cacgactcg ctctgcgtc gaggtcctg cacgcaaga 
      gacataatc cccttggac ccgaacggc ttcagcgac 
      cccttcgtc ataatcgaa ctcttgccg caccgagtt 
      ttcccccat tgcaacgaa caacaaacc aacgtacac 
      aagaaaacc cttcatcca attttcgac gaatgcttc 
      gagttcagt gtttcctta gagcagtgt cggtctccc 
      ggagccatg atcgccttc acggtcatg gaccacgac 
      gtgctcacg gcgaacgac tttgccgga gaggccttc 
      ctggccttg gggagcatt ccgggagtc gccgacacc 
      accggagtg gacaacttc cacggtctc aaaccggtc 
      gagctaatt cttatgcag caacaccaa aaaaatcac 
      cctattcta caaatactg gaatctcga gtggctgat 
      cgattagcc gccgaattc gtacgaaaa cagagacag 
      agaatcgca aataaatag
    mRNA sequence
    • >TC002977 RA
      tcgaacggc cgcgataat gtgaactgt tgatcctcg 
      ctcacgata ctcttgttt atcctttac ggcatggtg 
      taaagaaag tgaaaaacg ttggcaata tgagcttct 
      tcaactcct tacaagagt atgtcagca atagcgtcg 
      ccggactca gcctaagcc caaaacgat tttccttaa 
      gcagaagtg acacggcgg aacccccta cgggaattc 
      ccccgggat cgcgtagca attcaggtg atagcaaca 
      ctcccggtt ttcccaaag ttctcccgg cacccggtg 
      ccagtgctc tcgtccagc gacgccgca gcactttag 
      aatgcctcg tacctgcac ctcctgtcc gaaggcccg 
      gctccttca ggcagcgtt cgccgaaag ccactcctg 
      acaaacggc cttccttct gcagcagaa ggctgagct 
      ggcccgaca tcgatcatc aggttcgtt cgggagttc 
      aagaaattg acggctctt atttcgaga gcttcacag 
      cgctggcat ggaaacaag aaaatcaac gacagagtg 
      tgatgaaag cggccgaag tcgctgctg cggaccctc 
      cgccccagg aagccgatt tgggcattg tggccattg 
      actacacag cggcccaaa atgaaaagg acaagctct 
      acgtggaaa tgctttaca ccattgcca acacggtcg 
      gtgctcccg ccccaggag gccaatatg cccattaca 
      aagaagacc tctatttgt acggccaaa gggcgttcg 
      ggatgccgc cagaccgtc actaccgca tgctgcacc 
      tcgcaggag aggaaaaac caccgattg ttgttctca 
      gcgtcgttg tgatagaag ctgaaggac tcgaagcca 
      aagatgcca atggtttca gtgacccgt actgcatgc 
      tcggaatcc aaccgatgg cagcgccgt cctctcctc 
      agccccagc cgctcaccc ctaatcgca ccctgtcgg 
      atgcttgcg cccccgaca tgcagggca atcacgaca 
      agctccgga agcaccaca gcttcaaac tcagcttta 
      aaagaaaag acggacgag ttagggaac aacgggaca 
      gcatcgtcg gtgcactgc ccgctaaat tcattcgcg 
      ccacctcgg tcaaacccc acacgctta atccgaaat 
      ggaacgaga agttcagat ttgacatag acgacatca 
      acagcgact ccttgcacc tcgacatat gggaccacg 
      acgacgaaa gttcagtgc tagaagcag tcagcaaac 
      taaacgagg ttcgcggag ttcggggtt tgggccgat 
      ttttcaagc aagtttgcc aatctgcgc gacaaagtt 
      cccaagacg atttcctcg gatgtgtta cgattcccc 
      tccaagaca ttccatcga caggcttgg aaggctggt 
      tcaagctcg aagctcgat ctcagcgca gctcggtcc 
      agggccgca tccgcctga aaatgtggc tctcgaccc 
      gcgagaacc gcggcgcct ccgaggagg acaactgga 
      ccgagctca tccagcacg agagcctct acgccacgt 
      ttgtcgact acgagctaa aaaactgga accgcgagt 
      cctggacct ggagcgggg acctgccag ggccggcgc 
      tcacgattc tgcaccagc acgcggtcc aaggggatt 
      tgacggagc ttcaaaccg ccgtggccc gctttgtgg 
      cggcctgca gggtctacc tgaagcatc cgctggatc 
      cccgctgga tgctccagt tactgacag acattgaga 
      gcgcttgga tgaatttca ctctgacaa gagaggagg 
      aaatgtggc tcgctgaga agtttacgg caatgcaag 
      agagatggt tgcaacagt tgcgccacc accgacagt 
      tgttcccag ccctccatc ccccgtctc tggttcgac 
      ttgaatacg ttctgcgat gccttgcct acatgtcga 
      atatgaaag cattctgga aatgctgcc ccttcaata 
      aggaaatcc gcggtgaga tagtggcca ccctccgca 
      aagggacac ctgaatggt ttcaaagcc taaaatcgt 
      ctattatga cctcggatg aatacgacg cctgttttg 
      ttgacttct gctcggagg tttatgtac atttgcaac 
      acggactat ctcattatc atcctctct ttgagggca 
      caaacggaa tcccctact tcagcgtgg tgtttaaac 
      agctagata agttaatgt cagacgagg taatcgcat 
      ttctgaacc aaaacgagc acccggacg cagcttaca 
      gccgactta tcttttctg tttacttgg aagtgaaag 
      atttggcca cttttcatc aaaatctgc cgtccggcg 
      gcgatcata aactgttat tagtccgtt gtcatgaat 
      ggtttgagg cgtctgtta gttgttggt tgattgtat 
      gcaaagcga aggctttac aaagggtac gaactgcag 
      tcgaacttc aaaagccct gcgaagggg acaaattag 
      tgaagcaca gttcgtctg cagttgatg tagtagcga 
      tgttttgtc aattaagag acttctgga gactccttc 
      aatggccta aggcgcatt ccgttcctc ttctctcgc 
      aacttctcg actgtatat gttcagcag cgctattat 
      acgccgata tatcgtatc aagggttga tggaaacgg 
      ggtatttcg accggatgg ggcccttta aaatttgcg 
      acgaaatgt gcatgacgg tcaataact tggaatatg 
      tttacaagt ttgtgtctt tattagaaa attactttg 
      attttgtta cactcgaaa cgatcgctt cggaaatac 
      aatgcgcta ctttaacta accaattag atagcacac 
      taagtcagt tccaagtaa gaataatgg acataataa 
      agcgtgtag ggccacaaa tgcaagaag cacttagga 
      aagccatgt ttcatgtcg cttggtctc cagacactc 
      tacctacaa atcaagccg tggagccgc tgcttgaat 
      atctgcaca cacatctac atgcactca acttgtact 
      tgttgcctc agaacttcg aacgagtcc tatacgacg 
      tctgggagt acactttat ctgaattaa accatcaaa 
      tggatggtg gcacaaaca ctgacgaga tgcccccaa 
      tgttccacg agagactcc acgcggcgt tggagctca 
      tggttgact tcttccact gcgagggaa atgcgctta 
      gtatagaaa gcttgcatt catccatat tctgtcaaa 
      tcgaacaaa gactgcagt atcacagga cggatacgg 
      aaaccctca tagaaattt tctacaacc aaaggctcc 
      aagaacaac taaacacga ccagcagcc cgtacggga 
      gtctcgcag tccgagcgt acttcaacc acgactcgc 
      tctgcgtcg aggtcctgc acgcaagag acataatcc 
      ccttggacc cgaacggct tcagcgacc ccttcgtca 
      taatcgaac tcttgccgc accgagttt tcccccatt 
      gcaacgaac aacaaacca acgtacaca agaaaaccc 
      ttcatccaa ttttcgacg aatgcttcg agttcagtg 
      tttccttag agcagtgtc ggtctcccg gagccatga 
      tcgccttca cggtcatgg accacgacg tgctcacgg 
      cgaacgact ttgccggag aggccttcc tggccttgg 
      ggagcattc cgggagtcg ccgacacca ccggagtgg 
      acaacttcc acggtctca aaccggtcg agctaattc 
      ttatgcagc aacaccaaa aaaatcacc ctattctac 
      aaatactgg aatctcgag tggctgatc gattagccg 
      ccgaattcg tacgaaaac agagacaga gaatcgcaa 
      ataaataga taatctagt gtaatataa taatgtttt 
      gtacatctc tcttcctac gaaatggtt gtcaaaaat 
      tcaagcgct attttttct acgtgatgt gtctgtgag 
      acaaactaa caattttcg aacaattcg cgcactgaa 
      catcatgta catatttaa atgtaatac aagtatttt 
      ttatatgtt ccactatta cttatccaa agttacggc 
      ggaaatgtt ggtttgtcg gctgcaaaa tacttattg 
      ttcagtcta gagactttt ctaatgtaa gaactactt 
      ttctatgac tgataacgt gttagagct agatttctt 
      acaataaca cgtacaaac aacgtcaaa aatgccagg 
      gctttttat ttctacggt tgccgaatc aaaatactg 
      atcatatca tcgcagaac gaaccataa ctcgtattt 
      ttttaaacc actgtaggc cttttggcc aaaaaactc 
      cagacaatc cgcacacaa cgcataaaa gcgatttct 
      acgcaatct ggcattttg gatgaaaaa aattgatgt 
      tgcccttac tagaatatg aacctgcga tttaagtgc 
      actcctatt ttggctttc tttgatatg tgaagtcta 
      tacaatcat ttggacatc tttttaact gatgttaaa 
      agagtaaat acgatgtga atttgacta aactatcgc 
      aactttaaa taaaaatct aaatacgac agtaataca 
      cttgacgcc tgttacacg tgttaggaa acctctact 
      tatgtatgt agtacgcgt tgttcttca taattttat 
      tactgtcgt gcataaaat tatgaaaat aattaaatc 
      gtaaatcca taatataaa aatgtagta cgttattaa 
      cgcacatga aacaatcat ataattatt aaaaaagat 
      aataattta tatatacac tgttgttca taagagttt 
      atatgaatt gtaaagaac ctatacata tcttgcaca 
      ctttaccac gcggcaatc aaaatcctt gcttattta 
      tttattaac gctttccga ctgtgtgat aaaatataa 
      tgaagcttg agctttcag actaaattt attttttca 
      ccccttcat aacgagctt aatgtactg tatgtcaat 
      tgaaatcaa aatattgta ttctatata tttaattaa 
      tgtgatata tcttgtctt tgtctacaa atcaataaa 
      cccaaataa atacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY