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Gene information
General information
TC003451 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5300549..5308314
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003451
    iBeetle screen iB_06923

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003451 RA
      MANNNNEGA ENSPDNSKD AGLDKSFVG KFVSGLFNS 
      TPLSKWFGK DSASKSVKR REDDDDDDD DDFDASYVF 
      QPPSKRVKL PSEVEHNNY SAHIFESPV TVHNASNNV 
      NNVSSKVFG KFPEPVAGP SGVKSRKLF DRHRSASTN 
      TVSTNSFEN QEIVNGEND SDSEESTSG YSSAPKLLN 
      QPKSAEISH KETCEKVTP KTRSLFTSS NESSGRTLF 
      SERSPNMNT SLSSRRPSF NASTFGSPN FIDRTLSTK 
      RILSSPFYS GNTIYGGAS AYGRRLGKI SEETNSRVS 
      VQIKPVNEK PPSENATLS KTARRILST LEQYSTPIS 
      DAKKIPVTP RRPGLLSSY VGATPYLVR DRKRPSNKE 
      LQVPSVPDL LQMKQKERL QNSTETVRQ IATKSKSSL 
      NKEEYKIRT EEDNKQKHS NKMKSSVAS VRQKVPPIE 
      PVSEVKLPK VSLPISTLP KFDFVLMPP SNSSETTKN 
      QNETKTPKS PVSTVIKPT VIVDKKETE KKIVENGKS 
      SFTFSEPLV ISKDLKSVT AINCFKFSE PVCKKQSTH 
      TGLMFKSAE SKSEIKPKN NGENVPSIA KPASQLSTG 
      SVMDVLKKP PSQRWECPT CLVRNDIDK DKCCACDEP 
      KPQPEKKET KGFGEQFKM SSDKWECNS CMVRNNNSD 
      KTCVACGSS KAGEDKPVA KSGFGDAFK PPASTWECT 
      SCLIRNKNE LESCAACGA SKTPSGSFG DKFKPSGDT 
      WECATCMIR NKNTVDKCA ACETPKPGA KPTSVKPMT 
      APIFSFGIK QEWECKTCL IKNKNELTQ CAACEMPRE 
      SETEKKGFG DAFKMKGGE WECSSCLVK NKPTDNVCV 
      CCGVAKSGG KSSDVTTGE KKPLIGFNF GIDKSNAPQ 
      FKFGIPSTS SELKASTTS APPTFAFGD AAKSTAVTS 
      SSFSFGINT QEKVTPTNT VTSSENVTV ASNPLLKPS 
      EKLPEKATP APVGFKFGL DKTQESVVT SSSDSTNKT 
      ETPTTTSSF GTKPAPMFQ FKPPAVNGD IKFGSENKD 
      KPVLATEPV KESPFSAEP KNQDSPFGS KTQSSPFAT 
      AASKPQESP FGTPSKPTE SPFVSNKPA DLPFGSASK 
      PSDSPFGSA SKPKDSLFG NVNKPSSSL FGNTDANKP 
      KESIFGSTT EPNKPQAGL FTTVDTNKP QETLFGGDA 
      NKPKGGAIR HQTHRKQ
    • >TC003451 RB
      MANNNNEGA ENSPDNSKD AGLDKSFVG KFVSGLFNS 
      TPLSKWFGK DSASKSVKR REDDDDDDD DDFDASYVF 
      QPPSKRVKL PSEVEHNNY SAHIFESPV TVHNASNNV 
      NNVSSKVFG KFPEPVAGP SGVKSRKLF DRHRSASTN 
      TVSTNSFEN QEIVNGEND SDSEESTSG YSSAPKLLN 
      QPKSAEISH KETCEKVTP KTRSLFTSS NESSGRTLF 
      SERSPNMNT SLSSRRPSF NASTFGSPN FIDRTLSTK 
      RILSSPFYS GNTIYGGAS AYGRRLGKI SEETNSRVS 
      VQIKPVNEK PPSENATLS KTARRILST LEQYSTPIS 
      DAKKIPVTP RRPGLLSSY VGATPYLVR DRKRPSNKE 
      LQVPSVPDL LQMKQKERL QNSTETVRQ IATKSKSSL 
      NKEEYKIRT EEDNKQKHS NKMKSSVAS VRQKVPPIE 
      PVSEVKLPK VSLPISTLP KFDFVLMPP SNSSETTKN 
      QNETKTPKS PVSTVIKPT VIVDKKETE KKIVENGKS 
      SFTFSEPLV ISKDLKSVT AINCFKFSE PVCKKQSTH 
      TGLMFKSAE SKSEIKPKN NGENVPSIA KPASQLSTG 
      SVMDVLKKP PSQRWECPT CLVRNDIDK DKCCACDEP 
      KPQPEKKET KGFGEQFKM SSDKWECNS CMVRNNNSD 
      KTCVACGSS KAGEDKPVA KSGFGDAFK PPASTWECT 
      SCLIRNKNE LESCAACGA SKTPSGSFG DKFKPSGDT 
      WECATCMIR NKNTVDKCA ACETPKPGA KPTSVKPMT 
      APIFSFGIK QEWECKTCL IKNKNELTQ CAACEMPRE 
      SETEKKGFG DAFKMKGGE WECSSCLVK NKPTDNVCV 
      CCGVAKSGG KSSDVTTGE KKPLIGFNF GIDKSNAPQ 
      FKFGIPSTS SELKASTTS APPTFAFGD AAKSTAVTS 
      SSFSFGINT QEKVTPTNT VTSSENVTV ASNPLLKPS 
      EKLPEKATP APVGFKFGL DKTQESVVT SSSDSTNKT 
      ETPTTTSSF GTKPAPMFQ FKPPAVNGD IKFGSENKD 
      KPVLATEPV KESPFSAEP KNQDSPFGS KTQSSPFAT 
      AASKPQESP FGTPSKPTE SPFVSNKPA DLPFGSASK 
      PSDSPFGSA SKPKDSLFG NVNKPSSSL FGNTDANKP 
      KESIFGSTT EPNKPQAGL FTTVDTNKP QETLFGGDA 
      NKPKGATPT FTFTPKPPE SAPSLPSAA PAAGIFKFG 
      GATASPNQL STKRAFDEP ADNNVTNGF GSVPKIPAF 
      GASQSFGTS ESKPFAFES APKNAGFSF GQAEKPNTT 
      PVFNFGSAA AGASSAPNT GFSFSVPSA GFNFGGAPK 
      VESQPFNAG GSVFGTAGP AAKNGSFNF GTNANNNNQ 
      KAVFSFGAN QTNPPPSQP SFPSQGQSQ PGFNFGAPT 
      QPLNVPGGF NFTGAPPTF NFTDGNAGT APPQRKIKK 
      AFRRTTQR
    CDS sequence
    • >TC003451 RA
      atggcaaat aataataat gaaggtgcc gaaaacagt 
      ccagacaac tcgaaagat gcaggattg gataagtcg 
      ttcgtgggc aagttcgtg tcggggctt ttcaacagc 
      acccccttg tccaagtgg ttcggcaaa gactcggcg 
      tccaagtcg gtcaagagg cgggaggac gacgacgac 
      gacgacgac gacgacttt gatgcctcc tacgttttc 
      cagccacca agcaaacgt gtgaaacta ccgtctgaa 
      gtggaacac aacaattac agtgcacat atatttgaa 
      tcgccagtg actgtgcat aatgcttcc aataatgtt 
      aacaatgtt tcgtcgaaa gtgtttggg aagtttcct 
      gaaccggtg gcggggccg tcgggggtt aaatcccgc 
      aaattgttc gacagacac cgttctgct agcacaaat 
      acggtctct accaattcg ttcgaaaat caagaaatt 
      gttaacggt gagaatgac tctgacagt gaagaatca 
      actagtggc tattcttcg gcacccaaa ttgcttaat 
      caacccaaa agtgcagaa atatctcac aaggaaaca 
      tgtgaaaaa gtcactcct aaaactagg tccttgttt 
      acttcatca aacgaatca agcggacgt actttattc 
      tcggaaaga agccccaac atgaacact tctttgagt 
      tcaagacgg ccatcgttt aacgcatcg actttcggc 
      tcgccgaac tttattgac agaacattg tctaccaag 
      agaatattg agctcacct ttctatagc ggcaataca 
      atttatggc ggcgcctct gcatacgga cgtaggtta 
      ggtaaaatt agcgaagaa acgaattct agagtctct 
      gtacaaata aaacccgta aacgaaaaa cctccaagc 
      gaaaatgcg accttaagt aagaccgca cgtagaatt 
      ctgagcact ttagaacaa tacagcact ccaataagc 
      gacgctaaa aaaatacca gtcacgccg agaagacca 
      ggattgctg tcgtcttat gtcggggca actccttat 
      ctagtgaga gaccggaaa cggccttcg aataaggag 
      ctccaagta ccgtccgtt cctgattta ttgcagatg 
      aagcaaaag gagcgacta caaaatagc acagagact 
      gttagacaa attgccaca aagtcgaag tcgtcgctg 
      aataaggaa gagtataaa atcaggacg gaggaggac 
      aacaagcag aaacatagc aataaaatg aagagcagc 
      gtggcctcg gtgcggcaa aaagtgcca cctattgag 
      ccagtcagt gaggtgaaa ttgccgaaa gtttcactc 
      ccaataagc accttacct aagttcgat ttcgtttta 
      atgccaccc agcaattca agcgaaacc accaagaat 
      caaaacgaa actaaaact cccaaatcc ccggttagt 
      actgtaatt aaacccacg gtgattgta gataaaaaa 
      gagactgag aagaaaatc gtggaaaat ggaaagtcc 
      agtttcaca ttttccgaa ccgttagtg atttcaaaa 
      gacttaaag tctgtaact gcgatcaat tgtttcaaa 
      ttcagcgaa ccggtatgt aagaaacaa tcaacgcac 
      acaggactc atgtttaaa tcggccgaa agcaagagc 
      gaaattaaa ccaaaaaac aacggcgaa aatgtccca 
      agtatcgca aaaccggct agtcaatta tcaacagga 
      agcgtaatg gatgtgctg aaaaagccg ccgtcacaa 
      agatgggaa tgtccgact tgtctcgtc cgaaacgac 
      atcgacaaa gacaaatgt tgtgcctgt gatgagccc 
      aaacctcag cccgaaaag aaggaaacg aaagggttt 
      ggcgaacaa tttaaaatg tcgagtgac aagtgggaa 
      tgcaactcg tgcatggtg cggaataac aattcggat 
      aaaacttgc gtggcttgt ggctcttcc aaagcagga 
      gaagacaaa ccggtggct aaatccgga tttggagat 
      gccttcaaa ccgcctgca tcgacctgg gagtgcacg 
      tcttgtcta attaggaat aagaacgaa ctggagagt 
      tgtgcagcc tgtggtgca tccaagacg cccagtggt 
      agttttgga gacaagttc aagccatcg ggagacacc 
      tgggagtgt gctacttgc atgattaga aacaaaaat 
      acggtggat aaatgtgcg gcttgtgag acccctaaa 
      ccaggtgcc aagcctacg agtgtgaaa cccatgaca 
      gcaccgata ttctccttc ggtatcaaa caagagtgg 
      gagtgcaaa acatgcctg attaagaat aagaacgaa 
      ctgacgcag tgtgcagcc tgtgagatg cccagagag 
      agtgaaacg gagaaaaaa ggttttgga gacgctttc 
      aagatgaaa ggaggagaa tgggagtgt tcttcgtgt 
      cttgtcaag aataaaccc acagataat gtttgtgtt 
      tgttgtgga gttgccaaa tctggtggg aaatcctcg 
      gatgtgacg accggggaa aagaaacct ttaattggg 
      ttcaacttc ggtatagat aaatcaaac gcaccccaa 
      ttcaagttt ggtataccg agcactagt agcgaacta 
      aaagctagt actacaagt gcgccacct acatttgca 
      ttcggcgat gctgcgaag tcgactgca gtaacaagt 
      agtagtttt agtttcgga ataaatact caggaaaaa 
      gttacgcca acaaatact gttacctca agtgagaac 
      gtgaccgtc gccagtaat cccttactt aaacctagc 
      gagaaattg ccggagaaa gcaacgcct gcgccggtt 
      ggtttcaaa tttggcctt gataagact caggagtct 
      gtagtgacg agtagtagt gatagtact aataaaacg 
      gaaacgccg actactacg agcagtttc ggaacgaaa 
      ccggctccc atgttccag ttcaaaccc ccggctgtg 
      aacggcgat atcaagttt ggtagcgaa aataaggac 
      aaaccggtg ttggccacc gaaccggtt aaggagagt 
      cctttcagt gccgaaccg aagaatcaa gactcacct 
      ttcgggagt aagacgcaa agttcccca tttgctacc 
      gccgctagc aagccccaa gagtcaccc tttggtacc 
      cccagcaag ccaaccgaa tcacccttt gtcagtaac 
      aaacctgca gatctccca ttcggaagt gcaagtaaa 
      ccgagcgac tcaccgttc ggcagcgcc agtaagccg 
      aaagactca cttttcggc aacgtgaac aaaccctca 
      agttctctc ttcggcaac accgatgcc aacaaacca 
      aaagaatca attttcgga agcaccacc gaaccgaat 
      aaaccccaa gccgggtta ttcaccact gtggacaca 
      aacaagccg caagaaacc ctctttgga ggcgacgcc 
      aacaaaccg aagggaggt gctattcgg caccaaacc 
      caccgaaag cagtaa
    • >TC003451 RB
      atggcaaat aataataat gaaggtgcc gaaaacagt 
      ccagacaac tcgaaagat gcaggattg gataagtcg 
      ttcgtgggc aagttcgtg tcggggctt ttcaacagc 
      acccccttg tccaagtgg ttcggcaaa gactcggcg 
      tccaagtcg gtcaagagg cgggaggac gacgacgac 
      gacgacgac gacgacttt gatgcctcc tacgttttc 
      cagccacca agcaaacgt gtgaaacta ccgtctgaa 
      gtggaacac aacaattac agtgcacat atatttgaa 
      tcgccagtg actgtgcat aatgcttcc aataatgtt 
      aacaatgtt tcgtcgaaa gtgtttggg aagtttcct 
      gaaccggtg gcggggccg tcgggggtt aaatcccgc 
      aaattgttc gacagacac cgttctgct agcacaaat 
      acggtctct accaattcg ttcgaaaat caagaaatt 
      gttaacggt gagaatgac tctgacagt gaagaatca 
      actagtggc tattcttcg gcacccaaa ttgcttaat 
      caacccaaa agtgcagaa atatctcac aaggaaaca 
      tgtgaaaaa gtcactcct aaaactagg tccttgttt 
      acttcatca aacgaatca agcggacgt actttattc 
      tcggaaaga agccccaac atgaacact tctttgagt 
      tcaagacgg ccatcgttt aacgcatcg actttcggc 
      tcgccgaac tttattgac agaacattg tctaccaag 
      agaatattg agctcacct ttctatagc ggcaataca 
      atttatggc ggcgcctct gcatacgga cgtaggtta 
      ggtaaaatt agcgaagaa acgaattct agagtctct 
      gtacaaata aaacccgta aacgaaaaa cctccaagc 
      gaaaatgcg accttaagt aagaccgca cgtagaatt 
      ctgagcact ttagaacaa tacagcact ccaataagc 
      gacgctaaa aaaatacca gtcacgccg agaagacca 
      ggattgctg tcgtcttat gtcggggca actccttat 
      ctagtgaga gaccggaaa cggccttcg aataaggag 
      ctccaagta ccgtccgtt cctgattta ttgcagatg 
      aagcaaaag gagcgacta caaaatagc acagagact 
      gttagacaa attgccaca aagtcgaag tcgtcgctg 
      aataaggaa gagtataaa atcaggacg gaggaggac 
      aacaagcag aaacatagc aataaaatg aagagcagc 
      gtggcctcg gtgcggcaa aaagtgcca cctattgag 
      ccagtcagt gaggtgaaa ttgccgaaa gtttcactc 
      ccaataagc accttacct aagttcgat ttcgtttta 
      atgccaccc agcaattca agcgaaacc accaagaat 
      caaaacgaa actaaaact cccaaatcc ccggttagt 
      actgtaatt aaacccacg gtgattgta gataaaaaa 
      gagactgag aagaaaatc gtggaaaat ggaaagtcc 
      agtttcaca ttttccgaa ccgttagtg atttcaaaa 
      gacttaaag tctgtaact gcgatcaat tgtttcaaa 
      ttcagcgaa ccggtatgt aagaaacaa tcaacgcac 
      acaggactc atgtttaaa tcggccgaa agcaagagc 
      gaaattaaa ccaaaaaac aacggcgaa aatgtccca 
      agtatcgca aaaccggct agtcaatta tcaacagga 
      agcgtaatg gatgtgctg aaaaagccg ccgtcacaa 
      agatgggaa tgtccgact tgtctcgtc cgaaacgac 
      atcgacaaa gacaaatgt tgtgcctgt gatgagccc 
      aaacctcag cccgaaaag aaggaaacg aaagggttt 
      ggcgaacaa tttaaaatg tcgagtgac aagtgggaa 
      tgcaactcg tgcatggtg cggaataac aattcggat 
      aaaacttgc gtggcttgt ggctcttcc aaagcagga 
      gaagacaaa ccggtggct aaatccgga tttggagat 
      gccttcaaa ccgcctgca tcgacctgg gagtgcacg 
      tcttgtcta attaggaat aagaacgaa ctggagagt 
      tgtgcagcc tgtggtgca tccaagacg cccagtggt 
      agttttgga gacaagttc aagccatcg ggagacacc 
      tgggagtgt gctacttgc atgattaga aacaaaaat 
      acggtggat aaatgtgcg gcttgtgag acccctaaa 
      ccaggtgcc aagcctacg agtgtgaaa cccatgaca 
      gcaccgata ttctccttc ggtatcaaa caagagtgg 
      gagtgcaaa acatgcctg attaagaat aagaacgaa 
      ctgacgcag tgtgcagcc tgtgagatg cccagagag 
      agtgaaacg gagaaaaaa ggttttgga gacgctttc 
      aagatgaaa ggaggagaa tgggagtgt tcttcgtgt 
      cttgtcaag aataaaccc acagataat gtttgtgtt 
      tgttgtgga gttgccaaa tctggtggg aaatcctcg 
      gatgtgacg accggggaa aagaaacct ttaattggg 
      ttcaacttc ggtatagat aaatcaaac gcaccccaa 
      ttcaagttt ggtataccg agcactagt agcgaacta 
      aaagctagt actacaagt gcgccacct acatttgca 
      ttcggcgat gctgcgaag tcgactgca gtaacaagt 
      agtagtttt agtttcgga ataaatact caggaaaaa 
      gttacgcca acaaatact gttacctca agtgagaac 
      gtgaccgtc gccagtaat cccttactt aaacctagc 
      gagaaattg ccggagaaa gcaacgcct gcgccggtt 
      ggtttcaaa tttggcctt gataagact caggagtct 
      gtagtgacg agtagtagt gatagtact aataaaacg 
      gaaacgccg actactacg agcagtttc ggaacgaaa 
      ccggctccc atgttccag ttcaaaccc ccggctgtg 
      aacggcgat atcaagttt ggtagcgaa aataaggac 
      aaaccggtg ttggccacc gaaccggtt aaggagagt 
      cctttcagt gccgaaccg aagaatcaa gactcacct 
      ttcgggagt aagacgcaa agttcccca tttgctacc 
      gccgctagc aagccccaa gagtcaccc tttggtacc 
      cccagcaag ccaaccgaa tcacccttt gtcagtaac 
      aaacctgca gatctccca ttcggaagt gcaagtaaa 
      ccgagcgac tcaccgttc ggcagcgcc agtaagccg 
      aaagactca cttttcggc aacgtgaac aaaccctca 
      agttctctc ttcggcaac accgatgcc aacaaacca 
      aaagaatca attttcgga agcaccacc gaaccgaat 
      aaaccccaa gccgggtta ttcaccact gtggacaca 
      aacaagccg caagaaacc ctctttgga ggcgacgcc 
      aacaaaccg aagggagca acacccact ttcactttc 
      acgccaaaa ccccccgag agcgccccg agtctgcca 
      tcggcggcc ccagcagcc ggcattttc aaattcggt 
      ggtgccacc gcctcgcca aaccaactt tcgaccaag 
      cgggctttc gacgagccc gctgacaat aacgtaacc 
      aatggcttc ggttcggtt ccgaaaatt ccagccttt 
      ggggcgtcc cagagcttc gggacgagc gaaagcaag 
      ccgtttgcg ttcgaaagt gcccccaaa aatgcgggt 
      ttttcgttc ggtcaggcc gagaaaccg aatacgacg 
      cccgttttc aattttggg agtgcagca gcaggggcg 
      tcaagtgcg ccaaataca gggtttagc ttctcggtg 
      cctagtgcc gggtttaac tttggtggt gcgcccaaa 
      gtggagagt cagccgttt aatgccggg gggagtgtt 
      ttcgggacg gcgggacca gcggccaag aatgggagt 
      ttcaatttt gggacaaat gcgaataat aacaatcag 
      aaagcggtg tttagtttt ggggctaat cagactaat 
      ccgccgccg agtcagccc agttttccc agtcaggga 
      cagagtcag ccggggttt aatttcgga gcaccgacc 
      caaccactt aatgtaccc gggggtttc aactttacg 
      ggcgcccca cctacattt aactttaca gatggaaat 
      gcaggaacg gcgccgccc cagcgaaaa atcaagaaa 
      gcgttccga cggacgacg cagcgatag
    mRNA sequence
    • >TC003451 RA
      gtgtggtga aagttgtca tagaaatgt gtgattttc 
      caaaattac tttatttgt gtaaaatag actaagaac 
      aattgtaat tatggcaaa taataataa tgaaggtgc 
      cgaaaacag tccagacaa ctcgaaaga tgcaggatt 
      ggataagtc gttcgtggg caagttcgt gtcggggct 
      tttcaacag caccccctt gtccaagtg gttcggcaa 
      agactcggc gtccaagtc ggtcaagag gcgggagga 
      cgacgacga cgacgacga cgacgactt tgatgcctc 
      ctacgtttt ccagccacc aagcaaacg tgtgaaact 
      accgtctga agtggaaca caacaatta cagtgcaca 
      tatatttga atcgccagt gactgtgca taatgcttc 
      caataatgt taacaatgt ttcgtcgaa agtgtttgg 
      gaagtttcc tgaaccggt ggcggggcc gtcgggggt 
      taaatcccg caaattgtt cgacagaca ccgttctgc 
      tagcacaaa tacggtctc taccaattc gttcgaaaa 
      tcaagaaat tgttaacgg tgagaatga ctctgacag 
      tgaagaatc aactagtgg ctattcttc ggcacccaa 
      attgcttaa tcaacccaa aagtgcaga aatatctca 
      caaggaaac atgtgaaaa agtcactcc taaaactag 
      gtccttgtt tacttcatc aaacgaatc aagcggacg 
      tactttatt ctcggaaag aagccccaa catgaacac 
      ttctttgag ttcaagacg gccatcgtt taacgcatc 
      gactttcgg ctcgccgaa ctttattga cagaacatt 
      gtctaccaa gagaatatt gagctcacc tttctatag 
      cggcaatac aatttatgg cggcgcctc tgcatacgg 
      acgtaggtt aggtaaaat tagcgaaga aacgaattc 
      tagagtctc tgtacaaat aaaacccgt aaacgaaaa 
      acctccaag cgaaaatgc gaccttaag taagaccgc 
      acgtagaat tctgagcac tttagaaca atacagcac 
      tccaataag cgacgctaa aaaaatacc agtcacgcc 
      gagaagacc aggattgct gtcgtctta tgtcggggc 
      aactcctta tctagtgag agaccggaa acggccttc 
      gaataagga gctccaagt accgtccgt tcctgattt 
      attgcagat gaagcaaaa ggagcgact acaaaatag 
      cacagagac tgttagaca aattgccac aaagtcgaa 
      gtcgtcgct gaataagga agagtataa aatcaggac 
      ggaggagga caacaagca gaaacatag caataaaat 
      gaagagcag cgtggcctc ggtgcggca aaaagtgcc 
      acctattga gccagtcag tgaggtgaa attgccgaa 
      agtttcact cccaataag caccttacc taagttcga 
      tttcgtttt aatgccacc cagcaattc aagcgaaac 
      caccaagaa tcaaaacga aactaaaac tcccaaatc 
      cccggttag tactgtaat taaacccac ggtgattgt 
      agataaaaa agagactga gaagaaaat cgtggaaaa 
      tggaaagtc cagtttcac attttccga accgttagt 
      gatttcaaa agacttaaa gtctgtaac tgcgatcaa 
      ttgtttcaa attcagcga accggtatg taagaaaca 
      atcaacgca cacaggact catgtttaa atcggccga 
      aagcaagag cgaaattaa accaaaaaa caacggcga 
      aaatgtccc aagtatcgc aaaaccggc tagtcaatt 
      atcaacagg aagcgtaat ggatgtgct gaaaaagcc 
      gccgtcaca aagatggga atgtccgac ttgtctcgt 
      ccgaaacga catcgacaa agacaaatg ttgtgcctg 
      tgatgagcc caaacctca gcccgaaaa gaaggaaac 
      gaaagggtt tggcgaaca atttaaaat gtcgagtga 
      caagtggga atgcaactc gtgcatggt gcggaataa 
      caattcgga taaaacttg cgtggcttg tggctcttc 
      caaagcagg agaagacaa accggtggc taaatccgg 
      atttggaga tgccttcaa accgcctgc atcgacctg 
      ggagtgcac gtcttgtct aattaggaa taagaacga 
      actggagag ttgtgcagc ctgtggtgc atccaagac 
      gcccagtgg tagttttgg agacaagtt caagccatc 
      gggagacac ctgggagtg tgctacttg catgattag 
      aaacaaaaa tacggtgga taaatgtgc ggcttgtga 
      gacccctaa accaggtgc caagcctac gagtgtgaa 
      acccatgac agcaccgat attctcctt cggtatcaa 
      acaagagtg ggagtgcaa aacatgcct gattaagaa 
      taagaacga actgacgca gtgtgcagc ctgtgagat 
      gcccagaga gagtgaaac ggagaaaaa aggttttgg 
      agacgcttt caagatgaa aggaggaga atgggagtg 
      ttcttcgtg tcttgtcaa gaataaacc cacagataa 
      tgtttgtgt ttgttgtgg agttgccaa atctggtgg 
      gaaatcctc ggatgtgac gaccgggga aaagaaacc 
      tttaattgg gttcaactt cggtataga taaatcaaa 
      cgcacccca attcaagtt tggtatacc gagcactag 
      tagcgaact aaaagctag tactacaag tgcgccacc 
      tacatttgc attcggcga tgctgcgaa gtcgactgc 
      agtaacaag tagtagttt tagtttcgg aataaatac 
      tcaggaaaa agttacgcc aacaaatac tgttacctc 
      aagtgagaa cgtgaccgt cgccagtaa tcccttact 
      taaacctag cgagaaatt gccggagaa agcaacgcc 
      tgcgccggt tggtttcaa atttggcct tgataagac 
      tcaggagtc tgtagtgac gagtagtag tgatagtac 
      taataaaac ggaaacgcc gactactac gagcagttt 
      cggaacgaa accggctcc catgttcca gttcaaacc 
      cccggctgt gaacggcga tatcaagtt tggtagcga 
      aaataagga caaaccggt gttggccac cgaaccggt 
      taaggagag tcctttcag tgccgaacc gaagaatca 
      agactcacc tttcgggag taagacgca aagttcccc 
      atttgctac cgccgctag caagcccca agagtcacc 
      ctttggtac ccccagcaa gccaaccga atcaccctt 
      tgtcagtaa caaacctgc agatctccc attcggaag 
      tgcaagtaa accgagcga ctcaccgtt cggcagcgc 
      cagtaagcc gaaagactc acttttcgg caacgtgaa 
      caaaccctc aagttctct cttcggcaa caccgatgc 
      caacaaacc aaaagaatc aattttcgg aagcaccac 
      cgaaccgaa taaacccca agccgggtt attcaccac 
      tgtggacac aaacaagcc gcaagaaac cctctttgg 
      aggcgacgc caacaaacc gaagggagg tgctattcg 
      gcaccaaac ccaccgaaa gcagtaaca tattcgcgg 
      gtccctcag caacaccca ctttcactt tcacgccaa 
      aaccccccg agagcgccc cgagtctgc catcggcgg 
      ccccagcag ccggcattt tcaaattcg gtggtgcca 
      ccgcctcgc caaaccaac tttcgacca agcgggctt 
      tcgacgagc ccgctgaca ataacgtaa ccaatggct 
      tcggttcgg ttccgaaaa ttccagcct ttggggcgt 
      cccagagct tcgggacga gcgaaagca agccgtttg 
      cgttcgaaa gtgccccca aaaatgcgg gtttttcgt 
      tcggtcagg ccgagaaac cgaatacga cgcccgttt 
      tcaattttg ggagtgcag cagcagggg cgtcaagtg 
      cgccaaata cagggttta gcttctcgg tgcctagtg 
      ccgggttta actttggtg gtgcgccca aagtggaga 
      gtcagccgt ttaatgccg gggggagtg ttttcggga 
      cggcgggac cagcggcca agaatggga gtttcaatt 
      ttgggacaa atgcgaata ataacaatc agaaagcgg 
      tgtttagtt ttggggcta atcagacta atccgccgc 
      cgagtcagc ccagttttc ccagtcagg gacagagtc 
      agccggggt ttaatttcg gagcaccga ccgtaagtt 
      aattgtttt gtgatggtg atttttttt taatttatt 
      gtttgcagc aaccactta atgtacccg ggggtttca 
      actttacgg gcgccccac ctacattta actttacag 
      atggaaatg caggaacgt aagtagttg tatttgttt 
      attacgaga gaagttaca agtgaatgt ttcggttta 
      gggcgccgc cccagcgaa aaatcaaga aagcgttcc 
      gacggacga cgcagcgat agtttccta gagtttgta 
      tatagagga gtgattgta tcgtgaaac aagaaattg 
      gagttgtgt gagtacgtg ttaagtttg cttaaatta 
      tgtaaagaa taaatgtgt ggatcaatc accttgata 
      ttaaaaaca atatttgtt ttcagacct ataaatgtt 
      agctaggca ttaataata aatgggcca cctgtgaat 
      attcgagtc caggcattc gtctttcga acatgtaat 
      ataacgttg aaaatcttc agaatttat ttaccatat 
      ctgaatgtc tgcagttac aagtaatgt tcacagatg 
      cgtcggtgt acaaatgac actgttgta cacggcatg 
      ttactccct acattgtat accatagcg taggcattt 
      caaaggtct gatccatag aatgtagaa ttttataat 
      gatgatgaa gaggatttt catcaatat tgtgtgatg 
      atgaatatg atgaaatat ttgtgaaat ttttgaatg 
      attcgtacc atataatat ttatatgac ggccaatac 
      tggctactt ttactgtga tattcttat gggtcaggc 
      cccatacat aagtataaa caatatgca tataaatat 
      aatatctaa actaat
    • >TC003451 RB
      gtgtggtga aagttgtca tagaaatgt gtgattttc 
      caaaattac tttatttgt gtaaaatag actaagaac 
      aattgtaat tatggcaaa taataataa tgaaggtgc 
      cgaaaacag tccagacaa ctcgaaaga tgcaggatt 
      ggataagtc gttcgtggg caagttcgt gtcggggct 
      tttcaacag caccccctt gtccaagtg gttcggcaa 
      agactcggc gtccaagtc ggtcaagag gcgggagga 
      cgacgacga cgacgacga cgacgactt tgatgcctc 
      ctacgtttt ccagccacc aagcaaacg tgtgaaact 
      accgtctga agtggaaca caacaatta cagtgcaca 
      tatatttga atcgccagt gactgtgca taatgcttc 
      caataatgt taacaatgt ttcgtcgaa agtgtttgg 
      gaagtttcc tgaaccggt ggcggggcc gtcgggggt 
      taaatcccg caaattgtt cgacagaca ccgttctgc 
      tagcacaaa tacggtctc taccaattc gttcgaaaa 
      tcaagaaat tgttaacgg tgagaatga ctctgacag 
      tgaagaatc aactagtgg ctattcttc ggcacccaa 
      attgcttaa tcaacccaa aagtgcaga aatatctca 
      caaggaaac atgtgaaaa agtcactcc taaaactag 
      gtccttgtt tacttcatc aaacgaatc aagcggacg 
      tactttatt ctcggaaag aagccccaa catgaacac 
      ttctttgag ttcaagacg gccatcgtt taacgcatc 
      gactttcgg ctcgccgaa ctttattga cagaacatt 
      gtctaccaa gagaatatt gagctcacc tttctatag 
      cggcaatac aatttatgg cggcgcctc tgcatacgg 
      acgtaggtt aggtaaaat tagcgaaga aacgaattc 
      tagagtctc tgtacaaat aaaacccgt aaacgaaaa 
      acctccaag cgaaaatgc gaccttaag taagaccgc 
      acgtagaat tctgagcac tttagaaca atacagcac 
      tccaataag cgacgctaa aaaaatacc agtcacgcc 
      gagaagacc aggattgct gtcgtctta tgtcggggc 
      aactcctta tctagtgag agaccggaa acggccttc 
      gaataagga gctccaagt accgtccgt tcctgattt 
      attgcagat gaagcaaaa ggagcgact acaaaatag 
      cacagagac tgttagaca aattgccac aaagtcgaa 
      gtcgtcgct gaataagga agagtataa aatcaggac 
      ggaggagga caacaagca gaaacatag caataaaat 
      gaagagcag cgtggcctc ggtgcggca aaaagtgcc 
      acctattga gccagtcag tgaggtgaa attgccgaa 
      agtttcact cccaataag caccttacc taagttcga 
      tttcgtttt aatgccacc cagcaattc aagcgaaac 
      caccaagaa tcaaaacga aactaaaac tcccaaatc 
      cccggttag tactgtaat taaacccac ggtgattgt 
      agataaaaa agagactga gaagaaaat cgtggaaaa 
      tggaaagtc cagtttcac attttccga accgttagt 
      gatttcaaa agacttaaa gtctgtaac tgcgatcaa 
      ttgtttcaa attcagcga accggtatg taagaaaca 
      atcaacgca cacaggact catgtttaa atcggccga 
      aagcaagag cgaaattaa accaaaaaa caacggcga 
      aaatgtccc aagtatcgc aaaaccggc tagtcaatt 
      atcaacagg aagcgtaat ggatgtgct gaaaaagcc 
      gccgtcaca aagatggga atgtccgac ttgtctcgt 
      ccgaaacga catcgacaa agacaaatg ttgtgcctg 
      tgatgagcc caaacctca gcccgaaaa gaaggaaac 
      gaaagggtt tggcgaaca atttaaaat gtcgagtga 
      caagtggga atgcaactc gtgcatggt gcggaataa 
      caattcgga taaaacttg cgtggcttg tggctcttc 
      caaagcagg agaagacaa accggtggc taaatccgg 
      atttggaga tgccttcaa accgcctgc atcgacctg 
      ggagtgcac gtcttgtct aattaggaa taagaacga 
      actggagag ttgtgcagc ctgtggtgc atccaagac 
      gcccagtgg tagttttgg agacaagtt caagccatc 
      gggagacac ctgggagtg tgctacttg catgattag 
      aaacaaaaa tacggtgga taaatgtgc ggcttgtga 
      gacccctaa accaggtgc caagcctac gagtgtgaa 
      acccatgac agcaccgat attctcctt cggtatcaa 
      acaagagtg ggagtgcaa aacatgcct gattaagaa 
      taagaacga actgacgca gtgtgcagc ctgtgagat 
      gcccagaga gagtgaaac ggagaaaaa aggttttgg 
      agacgcttt caagatgaa aggaggaga atgggagtg 
      ttcttcgtg tcttgtcaa gaataaacc cacagataa 
      tgtttgtgt ttgttgtgg agttgccaa atctggtgg 
      gaaatcctc ggatgtgac gaccgggga aaagaaacc 
      tttaattgg gttcaactt cggtataga taaatcaaa 
      cgcacccca attcaagtt tggtatacc gagcactag 
      tagcgaact aaaagctag tactacaag tgcgccacc 
      tacatttgc attcggcga tgctgcgaa gtcgactgc 
      agtaacaag tagtagttt tagtttcgg aataaatac 
      tcaggaaaa agttacgcc aacaaatac tgttacctc 
      aagtgagaa cgtgaccgt cgccagtaa tcccttact 
      taaacctag cgagaaatt gccggagaa agcaacgcc 
      tgcgccggt tggtttcaa atttggcct tgataagac 
      tcaggagtc tgtagtgac gagtagtag tgatagtac 
      taataaaac ggaaacgcc gactactac gagcagttt 
      cggaacgaa accggctcc catgttcca gttcaaacc 
      cccggctgt gaacggcga tatcaagtt tggtagcga 
      aaataagga caaaccggt gttggccac cgaaccggt 
      taaggagag tcctttcag tgccgaacc gaagaatca 
      agactcacc tttcgggag taagacgca aagttcccc 
      atttgctac cgccgctag caagcccca agagtcacc 
      ctttggtac ccccagcaa gccaaccga atcaccctt 
      tgtcagtaa caaacctgc agatctccc attcggaag 
      tgcaagtaa accgagcga ctcaccgtt cggcagcgc 
      cagtaagcc gaaagactc acttttcgg caacgtgaa 
      caaaccctc aagttctct cttcggcaa caccgatgc 
      caacaaacc aaaagaatc aattttcgg aagcaccac 
      cgaaccgaa taaacccca agccgggtt attcaccac 
      tgtggacac aaacaagcc gcaagaaac cctctttgg 
      aggcgacgc caacaaacc gaagggagc aacacccac 
      tttcacttt cacgccaaa accccccga gagcgcccc 
      gagtctgcc atcggcggc cccagcagc cggcatttt 
      caaattcgg tggtgccac cgcctcgcc aaaccaact 
      ttcgaccaa gcgggcttt cgacgagcc cgctgacaa 
      taacgtaac caatggctt cggttcggt tccgaaaat 
      tccagcctt tggggcgtc ccagagctt cgggacgag 
      cgaaagcaa gccgtttgc gttcgaaag tgcccccaa 
      aaatgcggg tttttcgtt cggtcaggc cgagaaacc 
      gaatacgac gcccgtttt caattttgg gagtgcagc 
      agcaggggc gtcaagtgc gccaaatac agggtttag 
      cttctcggt gcctagtgc cgggtttaa ctttggtgg 
      tgcgcccaa agtggagag tcagccgtt taatgccgg 
      ggggagtgt tttcgggac ggcgggacc agcggccaa 
      gaatgggag tttcaattt tgggacaaa tgcgaataa 
      taacaatca gaaagcggt gtttagttt tggggctaa 
      tcagactaa tccgccgcc gagtcagcc cagttttcc 
      cagtcaggg acagagtca gccggggtt taatttcgg 
      agcaccgac ccaaccact taatgtacc cgggggttt 
      caactttac gggcgcccc acctacatt taactttac 
      agatggaaa tgcaggaac ggcgccgcc ccagcgaaa 
      aatcaagaa agcgttccg acggacgac gcagcgata 
      gtttcctag agtttgtat atagaggag tgattgtat 
      cgtgaaaca agaaattgg agttgtgtg agtacgtgt 
      taagtttgc ttaaattat gtaaagaat aaatgtgtg 
      gatcaatca ccttgatat taaaaacaa tatttgttt 
      tcagaccta taaatgtta gctaggcat taataataa 
      atgggccac ctgtgaata ttcgagtcc aggcattcg 
      tctttcgaa catgtaata taacgttga aaatcttca 
      gaatttatt taccatatc tgaatgtct gcagttaca 
      agtaatgtt cacagatgc gtcggtgta caaatgaca 
      ctgttgtac acggcatgt tactcccta cattgtata 
      ccatagcgt aggcatttc aaaggtctg atccataga 
      atgtagaat tttataatg atgatgaag aggattttc 
      atcaatatt gtgtgatga tgaatatga tgaaatatt 
      tgtgaaatt tttgaatga ttcgtacca tataatatt 
      tatatgacg gccaatact ggctacttt tactgtgat 
      attcttatg ggtcaggcc ccatacata agtataaac 
      aatatgcat ataaatata atatctaaa ctaatcaac 
      acagacaaa acgaattat tgtttttat tgcaaaagt 
      ccatggtag aagactaga ttatttttt tacgttgta 
      atttggttt gttggaaga atttggcag ccaagttgt 
      ttatcaagg attgctgag tatcgaact agtaattat 
      tgcaatgca atagtattt attttttat tgttaattt 
      agtttttgt ctgtgttat cttatcaaa aagtaggct 
      cactcctta gttcctcaa attgaaaaa gttgtaaat 
      atttgttta aaagtgtgg tttttaaca acacatcta 
      ttttgtata attatattc gtaggatat atttaaatg 
      aataatttt tgttgtctg aaatacttg tgttgtttt 
      acgtaatga gccgttatt gggctcttt ttatgtgag 
      aagtattaa aaggagtta gactgcttc gtgtaaata 
      ttgtaattc tacatgaaa taaaacgtt ttgctgaaa 
      atttcattt ttacttcag ctattgtta ttcctaatt 
      catttatca cgaggttgc gttactttt tacgaacgg 
      atggaattt tcagcatat ttgtgcctt tattttttt 
      aatgtataa ttttattag ttatatttg tcaagaact 
      tggcctagt tttgtttaa attattttc gtttgggta 
      gttttacat atttatgtt gaatgtgaa gtttacaat 
      ggaaaacaa ctgacagga gcaccctat tttttaatg 
      ttccaaatc attcctact acgctagta tctttttaa 
      attatgcaa taatttata cttaataaa gccaagttt 
      tgttaaatt ttgttttag ttaataaaa ttacattag 
      ttat
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