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Gene information
General information
TC003445 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5204377..5206293
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003445
    iBeetle screen iB_06922

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003445 RA
      MNLDDYNHG ENEMEVEGS EDYPLQIIS PRVEEIHPL 
      ALVAINVDN NEPPATYND VGFPLVPQA NEIERLSPT 
      ESVASTPQT FDEESNQSR KRTFEDEER EEDGSVCPI 
      CLDEWTNTG PHRICALKC GHLFGEQCI TRWLNQNSR 
      KTCPTCKEK ARRHDIRYI YAKKLTAID TTELDNIKK 
      QLDSVTEQR NKFQTELAT CAYREQLLR QEIAQLKTQ 
      LRQNNVPTF NQPMVERVK LYMDKAMEI CKFGGSRVF 
      DARCELDLL MASMKSPTN LFAGFGLKK INISGYRTI 
      AFIPVHTLQ IRDICFHKE QNWVLTASM DKTFKVINN 
      HENSVVHQF THQMPLWSC CWDENNPYE LYVGTQAGS 
      VIKYDIRQL TEAVCTFSI EGDMSPVVS VASIPAAPG 
      EALAQGGVV SCKLNSLWL YENANGEYK RHPISLEGP 
      FVSMKYQTN TKQLLVSSR PNSRITYAR HTLSTLARN 
      EGKIDCNIL HTFQGGGMQ KLLSKSCFL SHTSDYVAA 
      HQENCKSVF LWSINTGQK VCSVTAHES VFDLKGFQN 
      QNGSFLVSL TDKKLEFFK FA
    CDS sequence
    • >TC003445 RA
      atgaacttg gatgactac aaccacgga gaaaatgag 
      atggaagtt gaaggctct gaggactac cccttgcag 
      ataatctcc ccgcgcgtg gaggagatc cacccctta 
      gcattagta gcaataaac gtagataat aacgagccc 
      cccgccacc tacaacgac gtggggttc cccctagtg 
      ccacaagcc aacgaaatc gaaagactg tcccccacc 
      gagtcagtg gcgagcacc ccccagacc ttcgacgag 
      gagagcaac cagtcgcga aagcgcacc ttcgaggac 
      gaggagcgc gaggaggac ggctccgtg tgccccatt 
      tgcctggac gagtggacg aacacgggc ccccatcgg 
      atatgcgcc ctcaagtgc gggcacctg ttcggtgag 
      cagtgcatc acccggtgg ctgaaccag aactcgcgc 
      aaaacgtgc ccgacctgc aaggagaag gcgcgccgc 
      cacgacatt cgctacatt tacgccaaa aaactaaca 
      gcaattgat acaactgaa ctggacaat atcaaaaaa 
      cagctggac tcggtgacc gaacagcgc aacaagttc 
      cagactgag ttggccacg tgcgcctac cgcgagcag 
      ttgctgagg caggagatc gcccagctc aagacgcaa 
      ctgaggcag aacaatgtt cctactttc aaccagccc 
      atggtggag cgcgtcaag ctgtacatg gacaaggcg 
      atggagatt tgcaaattc ggcggaagc cgggttttt 
      gacgcgcgc tgcgagctg gacctgctc atggcttcg 
      atgaagtcg cccacgaat ttgttcgcc gggttcggc 
      ttgaaaaaa ataaacatt tcggggtac agaacaatc 
      gcgttcatt ccagtccat acgttgcaa attagagac 
      atttgtttc cacaaggaa cagaactgg gtgcttaca 
      gcgtccatg gacaagacc tttaaggtc ataaacaac 
      cacgagaat tcggttgtc caccaattc acccatcaa 
      atgcccctc tggtcatgc tgttgggac gaaaacaac 
      ccatacgag ctatacgta gggacgcaa gctgggtcg 
      gttataaaa tacgacatt cggcaatta accgaggcc 
      gtttgtacc ttttccata gaaggggac atgtctccg 
      gtggtttca gtcgcgtcg atcccggca gccccgggc 
      gaagccctg gcgcaaggg ggcgtcgtg tcctgcaag 
      ttgaactcg ctctggctg tacgagaac gcgaatggg 
      gagtacaag agacacccg atttcctta gagggacca 
      ttcgtttca atgaaatac caaacaaac acaaagcaa 
      ttgttagtg tcttctagg cccaatagt cgaattacg 
      tacgcgagg catacttta agtaccttg gcgaggaat 
      gaggggaaa attgactgc aatattctg cacacgttt 
      caagggggc ggcatgcag aagttgcta tcaaagtcg 
      tgttttttg tcgcatacg agtgattac gttgctgcc 
      caccaggaa aattgcaag agtgtgttc ttgtggagc 
      atcaatacg gggcaaaag gtttgctcc gtgacggcg 
      cacgaaagt gttttcgat ctgaaaggg ttccagaat 
      caaaatggt agttttctg gtttcgttg actgataaa 
      aaactcgag tttttcaaa tttgcttag
    mRNA sequence
    • >TC003445 RA
      tgacgtcgc catttgaca ttgacagga tagtttttt 
      gaggttagg ttgtaaatt tatttgaaa aaaaaccca 
      aaaagtaat gaacttgga tgactacaa ccacggaga 
      aaatgagat ggaagttga aggctctga ggactaccc 
      cttgcagat aatctcccc gcgcgtgga ggagatcca 
      ccccttagc attagtagc aataaacgt agataataa 
      cgagccccc cgccaccta caacgacgt ggggttccc 
      cctagtgcc acaagccaa cgaaatcga aagactgtc 
      ccccaccga gtcagtggc gagcacccc ccagacctt 
      cgacgagga gagcaacca gtcgcgaaa gcgcacctt 
      cgaggacga ggagcgcga ggaggacgg ctccgtgtg 
      ccccatttg cctggacga gtggacgaa cacgggccc 
      ccatcggat atgcgccct caagtgcgg gcacctgtt 
      cggtgagca gtgcatcac ccggtggct gaaccagaa 
      ctcgcgcaa aacgtgccc gacctgcaa ggagaaggc 
      gcgccgcca cgacattcg ctacattta cgccaaaaa 
      actaacagc aattgatac aactgaact ggacaatat 
      caaaaaaca gctggactc ggtgaccga acagcgcaa 
      caagttcca gactgagtt ggccacgtg cgcctaccg 
      cgagcagtt gctgaggca ggagatcgc ccagctcaa 
      gacgcaact gaggcagaa caatgttcc tactttcaa 
      ccagcccat ggtggagcg cgtcaagct gtacatgga 
      caaggcgat ggagatttg caaattcgg cggaagccg 
      ggtttttga cgcgcgctg cgagctgga cctgctcat 
      ggcttcgat gaagtcgcc cacgaattt gttcgccgg 
      gttcggctt gaaaaaaat aaacatttc ggggtacag 
      aacaatcgc gttcattcc agtccatac gttgcaaat 
      tagagacat ttgtttcca caaggaaca gaactgggt 
      gcttacagc gtccatgga caagacctt taaggtcat 
      aaacaacca cgagaattc ggttgtcca ccaattcac 
      ccatcaaat gcccctctg gtcatgctg ttgggacga 
      aaacaaccc atacgagct atacgtagg gacgcaagc 
      tgggtcggt tataaaata cgacattcg gcaattaac 
      cgaggccgt ttgtacctt ttccataga aggggacat 
      gtctccggt ggtttcagt cgcgtcgat cccggcagc 
      cccgggcga agccctggc gcaaggggg cgtcgtgtc 
      ctgcaagtt gaactcgct ctggctgta cgagaacgc 
      gaatgggga gtacaagag acacccgat ttccttaga 
      gggaccatt cgtttcaat gaaatacca aacaaacac 
      aaagcaatt gttagtgtc ttctaggcc caatagtcg 
      aattacgta cgcgaggca tactttaag taccttggc 
      gaggaatga ggggaaaat tgactgcaa tattctgca 
      cacgtttca agggggcgg catgcagaa gttgctatc 
      aaagtcgtg ttttttgtc gcatacgag tgattacgt 
      tgctgccca ccaggaaaa ttgcaagag tgtgttctt 
      gtggagcat caatacggg gcaaaaggt ttgctccgt 
      gacggcgca cgaaagtgt tttcgatct gaaagggtt 
      ccagaatca aaatggtag ttttctggt ttcgttgac 
      tgataaaaa actcgagtt tttcaaatt tgcttaggc 
      ggtttttaa agtttaagt attaagtat taggtacac 
      tcttcaatt ttgttacgg atcacattt tgaccatac 
      gattttcaa taaattatt tattttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY