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Gene information
General information
TC003420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4805021..4809304
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003420
    iBeetle screen iB_06920

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003420 RA
      MCEVGENSR ILASTPSST TSGCLSNDE GSDDRLLPS 
      SLAAEELQL ALNKEATEE ELLKAITRC KDLVLESNQ 
      CSLERKWLV RHLIELRLR LQECREAME DPLHPRNKS 
      SGVSRRTVR GHHLNLQPL LSTSTKYCD HCTGTIWSL 
      VQAWYECED CGYACHYKC ISQIIRECA HVVASERGG 
      YELEICPEE GLSAQKYLC AECQSPLPI NWSEARKCD 
      YSGLYYCTA CHWGSSAVV PARVVHNWD LAPQPVCQA 
      SLQQLRVTA NRPLLNLEK LNPRLFSLV HELSLVRRL 
      RQELHGMRK YLSVCRNAN EDHLLWKHV DVPHLIETP 
      ELYSLQDLV DTHSGELPT KLHTIIDVF SKHIKVKCE 
      VCRGRGHLC EICSNDEVL FPFDAAAVI CSECNAVLH 
      KSCFGRKSK CPKCLRLQQ RKEQQLGDQ SEE
    CDS sequence
    • >TC003420 RA
      atgtgcgaa gtaggggaa aacagcaga attttagcc 
      tcaacgccg tcgtctaca acgtcgggt tgtttatcg 
      aatgacgaa ggttcagac gataggctc ctgccttca 
      agtctagcg gcggaagag ttgcaattg gctttaaac 
      aaggaagcc acagaggaa gagttgtta aaggctatc 
      acgcgatgt aaggacttg gttttggag agcaatcag 
      tgttcattg gaacgcaag tggctcgtg aggcacctc 
      atagaacta cgtttgagg ctacaagaa tgtagggaa 
      gcgatggag gatccacta catccacgg aataaaagc 
      agtggtgtg tcgaggaga acagtgcga gggcaccat 
      ttaaacttg cagccactt ctgagtact tccacaaag 
      tattgtgat cattgtact ggcacaatt tggagtttg 
      gtgcaagct tggtatgag tgtgaggac tgtggttat 
      gcgtgtcat tataaatgc atatcgcaa ataataaga 
      gaatgtgct catgtcgtt gccagtgaa aggggagga 
      tatgagttg gaaatttgc ccagaagaa ggtttatca 
      gctcaaaag tatctgtgc gccgagtgc cagtcacct 
      ctccccata aactggtcc gaagcccgc aaatgcgac 
      tactccggc ctttactac tgcacggcc tgccactgg 
      ggcagctca gccgtggtc cccgcccgc gtggtgcac 
      aactgggac ctcgccccc cagcccgtg tgccaggca 
      agtctgcag caactccgc gtaacagca aatcgcccg 
      cttttaaat ttagaaaaa ctgaaccca cgcttattt 
      tcgctcgtc catgagctg agtttagtg cgccgtttg 
      cggcaagaa ttacacgga atgaggaaa tatctctca 
      gtttgtcga aacgctaat gaagaccat ttgttgtgg 
      aaacacgtg gatgtgccg catttaatt gaaacgcca 
      gaactgtac agcttgcag gacctggtg gatacgcat 
      tcaggggag ctgccgacg aaacttcac acaatcatc 
      gatgtgttt agtaaacat atcaaggtg aagtgtgag 
      gtttgtcgg gggagagga catttgtgc gagatttgc 
      tcaaatgac gaagttttg tttcctttc gatgccgcg 
      gctgtgatt tgctcggag tgtaatgcc gtcctgcat 
      aagagctgt tttgggcgg aagagtaag tgcccaaag 
      tgtctgcgg ttgcaacaa cggaaggag cagcaatta 
      ggggatcag agtgaagaa tga
    mRNA sequence
    • >TC003420 RA
      gtcaaacaa gtcacaagt catcaacct gtttaatct 
      gaacattaa ttatcttaa catattcgc attaaaata 
      atatgtaaa caatgtgcg aagtagggg aaaacagca 
      gaattttag cctcaacgc cgtcgtcta caacgtcgg 
      gttgtttat cgaatgacg aaggttcag acgataggc 
      tcctgcctt caagtctag cggcggaag agttgcaat 
      tggctttaa acaaggaag ccacagagg aagagttgt 
      taaaggcta tcacgcgat gtaaggact tggttttgg 
      agagcaatc agtgttcat tggaacgca agtggctcg 
      tgaggcacc tcatagaac tacgtttga ggctacaag 
      aatgtaggg aagcgatgg aggatccac tacatccac 
      ggaataaaa gcagtggtg tgtcgagga gaacagtgc 
      gagggcacc atttaaact tgcagccac ttctgagta 
      cttccacaa agtattgtg atcattgta ctggcacaa 
      tttggagtt tggtgcaag cttggtatg agtgtgagg 
      actgtggtt atgcgtgtc attataaat gcatatcgc 
      aaataataa gagaatgtg ctcatgtcg ttgccagtg 
      aaaggggag gatatgagt tggaaattt gcccagaag 
      aaggtttat cagctcaaa agtatctgt gcgccgagt 
      gccagtcac ctctcccca taaactggt ccgaagccc 
      gcaaatgcg actactccg gcctttact actgcacgg 
      cctgccact ggggcagct cagccgtgg tccccgccc 
      gcgtggtgc acaactggg acctcgccc cccagcccg 
      tgtgccagg caagtctgc agcaactcc gcgtaacag 
      caaatcgcc cgcttttaa atttagaaa aactgaacc 
      cacgcttat tttcgctcg tccatgagc tgagtttag 
      tgcgccgtt tgcggcaag aattacacg gaatgagga 
      aatatctct cagtttgtc gaaacgcta atgaagacc 
      atttgttgt ggaaacacg tggatgtgc cgcatttaa 
      ttgaaacgc cagaactgt acagcttgc aggacctgg 
      tggatacgc attcagggg agctgccga cgaaacttc 
      acacaatca tcgatgtgt ttagtaaac atatcaagg 
      tgaagtgtg aggtttgtc gggggagag gacatttgt 
      gcgagattt gctcaaatg acgaagttt tgtttcctt 
      tcgatgccg cggctgtga tttgctcgg agtgtaatg 
      ccgtcctgc ataagagct gttttgggc ggaagagta 
      agtgcccaa agtgtctgc ggttgcaac aacggaagg 
      agcagcaat taggggatc agagtgaag aatgactgt 
      gatatagcc gtgtttttt cttttacgc acaagagat 
      aaccctatc agtataaaa ttatatcca tgtttgttt 
      caagctttt ataatacgt atgtattta tttaaataa 
      taaactatt gttacttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY