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Gene information
General information
TC003416 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4779051..4781305
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003416
    iBeetle screen iB_06919

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003416 RA
      MAQPSVAQY FNNRKRAAV DDYKSEYPQ KVLRLDQDA 
      TTHSPKTGA SNESKIVVK PPEASKVVK CTNLEQQNV 
      SRKVIKVPK KKLKAGSNQ DIQSLLNNM RKIERCVTP 
      PNPSEKDAN KELDHIKKK ICQSSKFKE LKASISRFK 
      QSAEQLKVA EKKTADVAK SPQLKTFKS IEFEVVVSP 
      QKVQSPEKP YLSPKKEPT VRRNLLSAL SPKKTLFNA 
      TDTTSKDTE SLTPQGLQL PFKYKTLTE LFRSIDTVS 
      QILFNRKEV ITFRKLKPA VEEMVKRNL LEKHLAQIK 
      SVYPTAYNF RQEKLKVFG TREERWELV LTPNLTNSE 
      DMTSQVLID RRRVFTETL IDKVKDYHN EFLLTLTPP 
      MIISKDKIT RWHPEFDLE KVPEIELGA LPEPPFEEK 
      FTTGKQVLE KARQMFNCN TRMEQALLK LKEAQQKAP 
      TASEGPPTQ VTSVLKGIP KALLEKVRQ KQAAKALVS 
      MTRTVDKEK EVQLYARLP EIARLTRNL FVTEKKTVL 
      ELDFVIDKL GNSYRTFLN KAEMENHLK AISNAVPGW 
      LVFHKLRSS VFLKLAKNA DLSQVLSKL EKISKEKNE 
      S
    CDS sequence
    • >TC003416 RA
      atggcacag ccttcagtg gcccagtat ttcaacaac 
      agaaaacgt gcggctgtc gatgattac aagtcagag 
      tacccccag aaagtgtta cgcctggac caagatgca 
      acgactcac tcaccaaaa actggggct tcaaacgaa 
      tccaaaatc gtagtgaaa ccaccagaa gcaagtaaa 
      gttgttaaa tgcaccaat cttgaacaa caaaatgta 
      tcaagaaag gttattaaa gtaccaaag aagaaactc 
      aaagctggg tcaaaccaa gacattcag agtcttctg 
      aataatatg cggaaaatt gagagatgt gtgactccg 
      ccaaatccg agcgagaaa gacgctaat aaggaattg 
      gaccatatt aagaagaaa atatgccag agctcgaag 
      ttcaaggaa ctcaaagct tccattagt aggtttaaa 
      cagtcagca gaacagctg aaagtagcg gaaaagaaa 
      actgcagat gtggccaaa tcgccacag ttgaagacc 
      ttcaagtca atagagttt gaagtcgta gttagtcct 
      cagaaagtc cagtcccct gagaagcca tacctgagc 
      cccaagaag gagcccacc gtccgtcgc aacctcctg 
      agtgccttg agccccaaa aagactctt ttcaatgca 
      actgacacc acgtccaaa gacaccgaa tctctaaca 
      cctcaaggc ttgcaatta ccattcaag tacaaaact 
      ctgaccgaa ctcttccgc tcaattgac accgtctcc 
      caaatctta ttcaatcgc aaggaagtg ataacattc 
      cgaaaactc aaaccagct gttgaagaa atggtcaaa 
      cgaaacctc cttgaaaaa catctcgcc caaatcaag 
      tcggtttat ccaaccgcc tacaacttc cggcaagaa 
      aaactcaaa gttttcgga actcgagaa gaacgctgg 
      gagttggtt ttaacccca aacctaacc aactccgaa 
      gacatgact tcgcaagtg ctcattgac cggagaaga 
      gttttcact gagactttg attgacaaa gtcaaggat 
      tatcacaac gaatttctc ttaaccctg acgccacca 
      atgataatc tcgaaggac aaaatcacc cgctggcac 
      cccgagttc gacttggag aaagtcccg gaaatcgag 
      ctcggagcc ctgccggag ccccccttc gaggagaag 
      ttcaccacc gggaagcaa gtcctggaa aaggcgcgc 
      cagatgttc aactgcaac acccgcatg gagcaggcg 
      ttgctgaag ctgaaggag gcgcagcag aaggccccc 
      acagccagt gaggggccc ccaacacag gtaacctcg 
      gttctcaaa ggtatcccc aaagcgttg ctcgagaaa 
      gtgcgccaa aagcaggcc gccaaagcg ctggtttcc 
      atgaccagg actgtagac aaggagaaa gaggtgcaa 
      ctttacgcc agattgccc gaaatcgcc cgcttgact 
      cgtaatttg ttcgttacc gagaaaaaa accgtttta 
      gagttagac tttgttatc gataagttg ggtaatagt 
      taccggact tttctgaat aaagcggaa atggaaaac 
      catttgaaa gcaatctca aatgctgta cctggctgg 
      ttggtattt cataaatta aggagttct gttttttta 
      aaactggcc aaaaatgcg gacttgagt caggtttta 
      agtaagttg gaaaagatc tccaaggaa aaaaatgaa 
      tcgtaa
    mRNA sequence
    • >TC003416 RA
      gcgcactgg tggaatttc ggttgcgcc ttcaccgaa 
      tccggaggg aaactaaat taacgacct ttctaattg 
      aagaaaatg gcacagcct tcagtggcc cagtatttc 
      aacaacaga aaacgtgcg gctgtcgat gattacaag 
      tcagagtac ccccagaaa gtgttacgc ctggaccaa 
      gatgcaacg actcactca ccaaaaact ggggcttca 
      aacgaatcc aaaatcgta gtgaaacca ccagaagca 
      agtaaagtt gttaaatgc accaatctt gaacaacaa 
      aatgtatca agaaaggtt attaaagta ccaaagaag 
      aaactcaaa gctgggtca aaccaagac attcagagt 
      cttctgaat aatatgcgg aaaattgag agatgtgtg 
      actccgcca aatccgagc gagaaagac gctaataag 
      gaattggac catattaag aagaaaata tgccagagc 
      tcgaagttc aaggaactc aaagcttcc attagtagg 
      tttaaacag tcagcagaa cagctgaaa gtagcggaa 
      aagaaaact gcagatgtg gccaaatcg ccacagttg 
      aagaccttc aagtcaata gagtttgaa gtcgtagtt 
      agtcctcag aaagtccag tcccctgag aagccatac 
      ctgagcccc aagaaggag cccaccgtc cgtcgcaac 
      ctcctgagt gccttgagc cccaaaaag actcttttc 
      aatgcaact gacaccacg tccaaagac accgaatct 
      ctaacacct caaggcttg caattacca ttcaagtac 
      aaaactctg accgaactc ttccgctca attgacacc 
      gtctcccaa atcttattc aatcgcaag gaagtgata 
      acattccga aaactcaaa ccagctgtt gaagaaatg 
      gtcaaacga aacctcctt gaaaaacat ctcgcccaa 
      atcaagtcg gtttatcca accgcctac aacttccgg 
      caagaaaaa ctcaaagtt ttcggaact cgagaagaa 
      cgctgggag ttggtttta accccaaac ctaaccaac 
      tccgaagac atgacttcg caagtgctc attgaccgg 
      agaagagtt ttcactgag actttgatt gacaaagtc 
      aaggattat cacaacgaa tttctctta accctgacg 
      ccaccaatg ataatctcg aaggacaaa atcacccgc 
      tggcacccc gagttcgac ttggagaaa gtcccggaa 
      atcgagctc ggagccctg ccggagccc cccttcgag 
      gagaagttc accaccggg aagcaagtc ctggaaaag 
      gcgcgccag atgttcaac tgcaacacc cgcatggag 
      caggcgttg ctgaagctg aaggaggcg cagcagaag 
      gcccccaca gccagtgag gggccccca acacaggta 
      acctcggtt ctcaaaggt atccccaaa gcgttgctc 
      gagaaagtg cgccaaaag caggccgcc aaagcgctg 
      gtttccatg accaggact gtagacaag gagaaagag 
      gtgcaactt tacgccaga ttgcccgaa atcgcccgc 
      ttgactcgt aatttgttc gttaccgag aaaaaaacc 
      gttttagag ttagacttt gttatcgat aagttgggt 
      aatagttac cggactttt ctgaataaa gcggaaatg 
      gaaaaccat ttgaaagca atctcaaat gctgtacct 
      ggctggttg gtatttcat aaattaagg agttctgtt 
      tttttaaaa ctggccaaa aatgcggac ttgagtcag 
      gttttaagt aagttggaa aagatctcc aaggaaaaa 
      aatgaatcg taactgatg acttgtgga gtaggttta 
      gcatgaagt tcaaaatta attattttt agtgtcctc 
      gtattgttt ccgtttcag tttctttta tgtgctgaa 
      tgtgattta gttgcggtg tcatttttt actgaataa 
      atgatattt ttaagaaaa tattgataa taaatgtat 
      cttttaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY