Status
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Gene information
General information
TC032368
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030706,TC030610
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4729981..4765814
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032368
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06918

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature lateral potentiallythinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen posteriorshape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032368 RA
      MADDHSHVP RAKARSASI CATSLETIP PMGAIPNGG 
      TPQSRRRKP ATRSQSARL NNNRSIKRR TLASANQME 
      MKGHCNSEP KLTDQAGVT PEVSPNIKR KGSARRGTS 
      VYHRKSTAF LDVPDTRAA RGNPEEEDE DSYRLRSFS 
      FTSKGIINR GDSFRRRRS RSNSLCPAV GGVMPSLLL 
      NEPEQTVPV CNYDVALLG ANGVGKTAL ISQFMTSEC 
      INAYDRQKD SLSGAQTVS IMLNGEESE LTFQNLSST 
      KEIDKSHPP DAFVMLYSV VDKASFLKA EIELTKLQD 
      ADLLRSRSV ILVGNKIDL ARSRAVSAQ DGKCLACTF 
      RAKFIEISV GINHNVDEL LVGILTQIR LKAASAEDN 
      DHQQQWYKS RGMMKASMK ARQMFTWLF GKEDSKFKN 
      CENLHIL
    CDS sequence
    • >TC032368 RA
      atggcggat gatcattcg cacgtgccg cgcgccaaa 
      gcccgatcg gcctccata tgcgccacc agcctcgag 
      accatcccc ccgatgggg gccatcccc aacgggggc 
      accccgcag agccgccgc cgaaagccg gccacgcgc 
      tcgcagagc gcccgcctc aacaacaac cgctccata 
      aagcggcgg acgttggcc tcagccaac caaatggaa 
      atgaagggc cactgtaac agtgaaccc aaactgacc 
      gaccaagcg ggggtcact cccgaagtc tcccccaat 
      atcaaaagg aaaggctcc gcaaggaga ggcacttcg 
      gtgtatcac agaaaatcc accgctttt ctagacgtg 
      cccgacaca cgtgctgcc aggggaaac cccgaagag 
      gaagacgag gattcgtac agattgagg tcgtttagt 
      ttcacttcc aaaggaata atcaataga ggagattcg 
      ttcagacgg agacgctca agaagtaat agtttatgt 
      cctgccgtc ggaggcgtc atgccttcc cttcttctg 
      aacgaaccc gagcaaact gttcctgtt tgcaactac 
      gatgtcgca ttacttgga gcaaatggc gtaggaaaa 
      actgcccta attagccag tttatgact tcagagtgt 
      ataaatgcg tacgatcga caaaaagat tcactgtca 
      ggagctcag actgtttcg ataatgctt aatggagaa 
      gaatccgaa ctgacgttt caaaatctt tccagtacg 
      aaggaaata gacaagtca catccacct gatgcattt 
      gtaatgctt tattccgtc gtagataag gctagtttt 
      ttaaaggcc gaaatagaa ctgaccaaa cttcaagat 
      gccgacctt ttgagatca cgatccgtt attttagtt 
      ggcaataaa attgactta gcaagaagt agagccgtt 
      tctgcacaa gatggcaaa tgtctggct tgcaccttc 
      cgggcgaaa tttatcgaa atctccgtg ggcataaat 
      cacaacgtg gacgaactc ctcgtcgga atattaacc 
      caaattaga ctaaaagca gcaagtgcc gaagacaac 
      gaccatcag cagcaatgg tacaaaagc cgaggtatg 
      atgaaagct agtatgaag gcaagacag atgtttacg 
      tggctgttt ggaaaagaa gattcgaaa tttaaaaat 
      tgtgaaaac ctccatata ctctag
    mRNA sequence
    • >TC032368 RA
      ctgggcttc tgaaagcgt cagtcagtg ctatggcgg 
      atgatcatt cgcacgtgc cgcgcgcca aagcccgat 
      cggcctcca tatgcgcca ccagcctcg agaccatcc 
      ccccgatgg gggccatcc ccaacgggg gcaccccgc 
      agagccgcc gccgaaagc cggccacgc gctcgcaga 
      gcgcccgcc tcaacaaca accgctcca taaagcggc 
      ggacgttgg cctcagcca accaaatgg aaatgaagg 
      gccactgta acagtgaac ccaaactga ccgaccaag 
      cgggggtca ctcccgaag tctccccca atatcaaaa 
      ggaaaggct ccgcaagga gaggcactt cggtgtatc 
      acagaaaat ccaccgctt ttctagacg tgcccgaca 
      cacgtgctg ccaggggaa accccgaag aggaagacg 
      aggattcgt acagattga ggtcgttta gtttcactt 
      ccaaaggaa taatcaata gaggagatt cgttcagac 
      ggagacgct caagaagta atagtttat gtcctgccg 
      tcggaggcg tcatgcctt cccttcttc tgaacgaac 
      ccgagcaaa ctgttcctg tttgcaact acgatgtcg 
      cattacttg gagcaaatg gcgtaggaa aaactgccc 
      taattagcc agtttatga cttcagagt gtataaatg 
      cgtacgatc gacaaaaag attcactgt caggagctc 
      agactgttt cgataatgc ttaatggag aagaatccg 
      aactgacgt ttcaaaatc tttccagta cgaaggaaa 
      tagacaagt cacatccac ctgatgcat ttgtaatgc 
      tttattccg tcgtagata aggctagtt ttttaaagg 
      ccgaaatag aactgacca aacttcaag atgccgacc 
      ttttgagat cacgatccg ttattttag ttggcaata 
      aaattgact tagcaagaa gtagagccg tttctgcac 
      aagatggca aatgtctgg cttgcacct tccgggcga 
      aatttatcg aaatctccg tgggcataa atcacaacg 
      tggacgaac tcctcgtcg gaatattaa cccaaatta 
      gactaaaag cagcaagtg ccgaagaca acgaccatc 
      agcagcaat ggtacaaaa gccgaggta tgatgaaag 
      ctagtatga aggcaagac agatgttta cgtggctgt 
      ttggaaaag aagattcga aatttaaaa attgtgaaa 
      acctccata tactctagc actagtttc gtttacggt 
      actacaatt attaatcaa ggtgttgta gataaacac 
      acaagtctt attcttgga cattacttc aaatttata 
      catcaataa ctacatcaa acagctctt tgcgttttg 
      tttatggct gcggtttac tcaaaatgt gggctaggg 
      ccaagacaa gatttgcgt gtttatatt attaacgca 
      ctgcttgtt ccaagaaag cagcaaatt gtccaattt 
      cttggagac aagcaccca attttacca acgttaggt 
      aaacaattc ttacagcag atgagcaaa tttgtgata 
      aataaggcc taccttcaa ccacaccca aattcattt 
      acagttagc tatcgttgg taaagtgag ccatgaagc 
      atatctcaa acatttgta ttaatgttg attcgttta 
      tgtttagta ttttctctg tgtgattat attttgtca 
      ctgctgact gactttaga tacaattat ttaaacaga 
      ttacgtgta cttcatcaa gtatatcta tatttatat 
      gaattatat gtcctgtaa caaaaatgt gggtaagac 
      ttcgcttcg aaagtaggt atattgtgt tatctatat 
      ctttgcatg tcgttatca tttttatcg cattgagga 
      cgaggaaac gtgatcctt gagggaatt gaggcggga 
      ggaaaactc aaatcaaaa ctgaaccat aaaacattt 
      ctttctgaa tgaaaaagc acactggaa actgcacta 
      aacagatga taatcatcc gtttattgg cgcgcgact 
      aatttgaaa tattaaagc agtgaaccg attaaccag 
      tagattgaa cccgggaga accccaaaa caaactgtc 
      gagtaagtt catttaata gctcgctga agatgttaa 
      gcaactaat tactatgca aatgcaaaa actggaaga 
      aaactgtgg acaaattga accatttca caccagcac 
      gtaattgtg ttttgtggc taccacccc agcaaggga 
      aaatcgccg tgaaaatgg attaaaata cgtaaatac 
      gctttgggc attccggca gcagaaccg atgattatt 
      ttataaatt gtgagagga cgtgaaata gttgcggcc 
      tgtggcgtt acaaggata tttaatatt cgcgagact 
      taattaaag tcgtattta gcaaagttc actctcttg 
      aaaagtttg gagttgaaa tgcgccttg tggacgttt 
      gtccattaa aattgagaa acttgtaca aattaacaa 
      actgcgcga agcatttaa ataatcgat ctgtttaca 
      aggaatgaa aaacgttaa ttctattat ctaagactt 
      gttacacaa cctacgaca tctttcaaa accgagtca 
      acaaagttc agggcaaat ctttgtatc aacagaccc 
      aagttccgc tccacaatc tcgtaatca atatgaacc 
      tgtggcaaa ttttgtcgc aactgaaaa tacaatact 
      gttctcctg tggagacat gtcacaaaa aatattccg 
      acaaatgag taccaaggt aagaaagtt acatattct 
      ctctcgaat gagcactaa ttgagcgaa agaaagaaa 
      atatttatg ctttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY