Status
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Gene information
General information
TC003407 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4431026..4448440
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003407
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06917

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03551

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003407 RA
      MMGYTRLSI LVILLVNTA TGGSLISHL DGSDQEERC 
      EDITIPMCM GIGYNQTRM PNELNHETQ EEAGLEVHQ 
      FWPLVEIKC SPDLKFFLC SMYAPICLP GYKKPLPPC 
      RGLCKRARE GCEPIMTQY GFKWPERMD CEQFPVYGA 
      SPDQLCMDE NNGSSTTAK PPPRAKAPK QCRGSKNCT 
      NPPGDGRKS SVAKGCKCQ CQHPLVSLG KDPSLLNLS 
      VLDPATCAA PCRATYFNS EEKEFTTVW ITLWSSLCA 
      ASTLMTLTT FFIETERFK YPERPIVFL SACYFLVSV 
      GYLIRVGVG HEEVACEGP VIRYSSTGA NPCSLVFLL 
      VYFFGMASS IWWVVLSFT WFLAAGLKW GNEAIASYA 
      EYFHVAAWL IPTLQTVAV FVSGAVDGD PVAGICYVG 
      NMNMENLRT FVLAPLFIY LLLGTTFLI AGFVSLFRI 
      RNVIKKQGG AGAGSKADK LEKLMIRIG IFSVLYTVP 
      ASIVIACHL YENAFHNEW MLSAACTCP SAKVRPIYS 
      VLMLKYFMA LAVGITSGV WIWSGKTLD SWRRLWRRL 
      FGRPDPTGA NAVLIKNRP KGLGGPPQY VVAGAGSAS 
      LLGPAGSVA SASQHHLHH HVLKQPPLS HV
    CDS sequence
    • >TC003407 RA
      atgatgggg tacacgagg ttatcaata ctcgtgata 
      ctcctagtg aacaccgca accggcggc tcgctaata 
      tcccacttg gacggcagc gaccaagag gagcgttgc 
      gaggacatc accatcccc atgtgcatg ggcatcggc 
      tacaaccaa actcgcatg cccaacgag ctcaaccac 
      gaaacgcaa gaggaagcc ggcctggaa gtgcaccag 
      ttctggcct ctggtcgag atcaagtgc tcccccgac 
      ttgaagttc ttcctctgc tccatgtac gcccccatc 
      tgcctcccc ggctacaag aagcctctg cccccctgc 
      cgcgggctg tgcaagcgg gcgcgcgag ggctgcgag 
      cccatcatg acccagtac ggcttcaag tggcccgag 
      cgcatggac tgcgagcag ttccccgtg tacggcgcc 
      tcccccgac cagctgtgc atggacgag aacaacggc 
      agcagcacc acggccaag ccgccgccg cgcgccaag 
      gcccccaaa cagtgccga gggtcaaaa aactgcaca 
      aatccccca ggagacgga agaaaaagc agcgtggcg 
      aagggctgc aagtgccag tgccaacac ccgctggtc 
      tctctgggg aaggacccg tcactgctc aacctaagc 
      gtcctcgac cccgccacc tgtgccgcc ccctgccgc 
      gccacgtac ttcaactcg gaggaaaaa gagttcacc 
      acggtctgg ataacactc tggtccagc ctgtgcgcg 
      gcttccacc ttaatgaca cttacgacc ttcttcatc 
      gaaaccgaa cggttcaag taccctgag cggccgatt 
      gtgttctta tcggcgtgc tattttctc gtctcggtg 
      ggttatctc atccgggtg ggggtgggg catgaagaa 
      gttgcgtgt gaaggaccc gttatccgc tattcctca 
      actggggcc aacccctgc tctttggtc ttcctccta 
      gtgtatttc tttggcatg gcctcgtcc atctggtgg 
      gtggtgctc tccttcacg tggtttcta gccgccggg 
      ttaaagtgg ggaaacgaa gctattgcc agctatgcc 
      gagtacttc catgtcgca gcttggctt attccaacc 
      ttgcaaaca gtcgcagtg tttgtctct ggggccgtt 
      gacggcgac ccggtcgcc ggtatttgc tacgttggc 
      aatatgaac atggagaac cttcgcacg tttgttctg 
      gcaccactt ttcatctac ctgctcctc ggtaccact 
      ttcctaatc gctggattt gtctccctc ttccgcatt 
      cgcaacgtg atcaagaaa cagggcggc gcgggcgcg 
      ggctccaaa gcggacaag ctggagaag ctcatgatc 
      cggatcggc atcttcagc gtgctgtac accgtcccc 
      gcgtccata gtcatcgcg tgtcacttg tacgaaaac 
      gcattccat aacgagtgg atgctctcg gccgcctgc 
      acctgcccc agtgcgaaa gtccgcccg atctactcc 
      gttctaatg ctcaaatac ttcatggcg ttggcggtg 
      ggcattacc tccggagtc tggatctgg tcgggcaag 
      acgctcgac tcctggcgg aggctgtgg aggcgcctc 
      ttcggccgc cccgacccc acgggggcc aacgccgtg 
      ctgatcaag aaccggccg aaggggctc ggggggccg 
      ccgcagtac gtggtggcg ggcgccggg agcgcgtcg 
      ctgctgggg cccgcgggc agcgtggcg tcggccagt 
      cagcaccac ctccaccat cacgtcctg aagcagccc 
      ccgttgagt cacgtatga
    mRNA sequence
    • >TC003407 RA
      gtattctca attttgttc agtcagtaa attcagttg 
      agcttggtt ggaggtgga gttaacgtc tgttgagtt 
      agtgttgga cgtaattta gcaccacca ccaaaataa 
      atagtttgc gaatatatc cctcaacac cggaggaat 
      tgtggagtg taaatattt aagtttaaa catcattca 
      gatttatgt tgtgcgaat tgaattttg aaatgttac 
      caagtgtag tgcgaactt agaatgctg ctcaggata 
      tacaaccca ctggaaaaa aatgataca gtttacaag 
      ggtgttatg gctccatcc tatgccttg ctattcatc 
      gccagtcgg caacaaaag tgagcccca accaaactc 
      ctgccttta gaactgaag tcgtgtgtg taaaataac 
      aatgatggg gtacacgag gttatcaat actcgtgat 
      actcctagt gaacaccgc aaccggcgg ctcgctaat 
      atcccactt ggacggcag cgaccaaga ggagcgttg 
      cgaggacat caccatccc catgtgcat gggcatcgg 
      ctacaacca aactcgcat gcccaacga gctcaacca 
      cgaaacgca agaggaagc cggcctgga agtgcacca 
      gttctggcc tctggtcga gatcaagtg ctcccccga 
      cttgaagtt cttcctctg ctccatgta cgcccccat 
      ctgcctccc cggctacaa gaagcctct gcccccctg 
      ccgcgggct gtgcaagcg ggcgcgcga gggctgcga 
      gcccatcat gacccagta cggcttcaa gtggcccga 
      gcgcatgga ctgcgagca gttccccgt gtacggcgc 
      ctcccccga ccagctgtg catggacga gaacaacgg 
      cagcagcac cacggccaa gccgccgcc gcgcgccaa 
      ggcccccaa acagtgccg agggtcaaa aaactgcac 
      aaatccccc aggagacgg aagaaaaag cagcgtggc 
      gaagggctg caagtgcca gtgccaaca cccgctggt 
      ctctctggg gaaggaccc gtcactgct caacctaag 
      cgtcctcga ccccgccac ctgtgccgc cccctgccg 
      cgccacgta cttcaactc ggaggaaaa agagttcac 
      cacggtctg gataacact ctggtccag cctgtgcgc 
      ggcttccac cttaatgac acttacgac cttcttcat 
      cgaaaccga acggttcaa gtaccctga gcggccgat 
      tgtgttctt atcggcgtg ctattttct cgtctcggt 
      gggttatct catccgggt gggggtggg gcatgaaga 
      agttgcgtg tgaaggacc cgttatccg ctattcctc 
      aactggggc caacccctg ctctttggt cttcctcct 
      agtgtattt ctttggcat ggcctcgtc catctggtg 
      ggtggtgct ctccttcac gtggtttct agccgccgg 
      gttaaagtg gggaaacga agctattgc cagctatgc 
      cgagtactt ccatgtcgc agcttggct tattccaac 
      cttgcaaac agtcgcagt gtttgtctc tggggccgt 
      tgacggcga cccggtcgc cggtatttg ctacgttgg 
      caatatgaa catggagaa ccttcgcac gtttgttct 
      ggcaccact tttcatcta cctgctcct cggtaccac 
      tttcctaat cgctggatt tgtctccct cttccgcat 
      tcgcaacgt gatcaagaa acagggcgg cgcgggcgc 
      gggctccaa agcggacaa gctggagaa gctcatgat 
      ccggatcgg catcttcag cgtgctgta caccgtccc 
      cgcgtccat agtcatcgc gtgtcactt gtacgaaaa 
      cgcattcca taacgagtg gatgctctc ggccgcctg 
      cacctgccc cagtgcgaa agtccgccc gatctactc 
      cgttctaat gctcaaata cttcatggc gttggcggt 
      gggcattac ctccggagt ctggatctg gtcgggcaa 
      gacgctcga ctcctggcg gaggctgtg gaggcgcct 
      cttcggccg ccccgaccc cacgggggc caacgccgt 
      gctgatcaa gaaccggcc gaaggggct cggggggcc 
      gccgcagta cgtggtggc gggcgccgg gagcgcgtc 
      gctgctggg gcccgcggg cagcgtggc gtcggccag 
      tcagcacca cctccacca tcacgtcct gaagcagcc 
      cccgttgag tcacgtatg acaagcggg cgagggggc 
      cacgcctgt agttgtctc acaccccgc acaaggttc 
      aggaccgtc gttgagcaa ctacgggcc catttgagt 
      gaccgcctt ggcccctcg acactcggt cacagctct 
      ctagtcttg catgtatgt tcgaggtgg acttttgta 
      cagacgatt gtgtttatt tatttgcta aacgtgtat 
      aaatgtgga aactttcac tgcattcgc atactcaaa 
      aattatcat gtgacaacg aatgacacg aaaaatcgt 
      aaccagcca tttttggcg gtaggactt gatctgata 
      atagtgata tatcgtagt caggcgcga aaatttatt 
      tggactttt acacgcaat tataccttc aacaatgtc 
      tatcgtaaa atttcatgg tgcatccat gtgaatgtc 
      aaaatatag aaagtgaga ttttatata cgtaattaa 
      actaattgg agtttacat tggtagtaa atgtgatcg 
      gtacgttag ttagatgag cgaatgttt agagtggta 
      ctctgactg aaattttcg tagaaaagt taacagtga 
      tattgccgt cgctattga cgctgtacg tatactcct 
      aactactta tccacagtg ttcagtgta gccatattt 
      tgtgccatg tataatcgt gtagggacc atccaagga 
      atttttctc ggagtacca tcaatcaaa gtgtgcact 
      ctttccgtt gacaatttg taaaatatt taatatatt 
      cttgtagtt ttcgaatag ctgtaggtt tttatatga 
      catattcat tgaccccaa taatctttt gtacttatc 
      tttgtctat ttaaccgtg taaatataa ctgtgcagt 
      tcatgaaat acgacaggt ttgctgtta ggtgtaaag 
      caacaatag ctatccatt gaaagtgga tgagtgtct 
      acgcatcca gtgatgatt tgtatccaa atacaatat 
      agctttata atgaattaa aaagatata attatagtg 
      tagaacata tcaaatttt atattaacg aaaattata 
      aatgcacaa tgtttacgt ttgtggata ctgccatta 
      cgaaattat tttggttga agaaacaca agcacaaaa 
      tgggttttg tatatattt tttcctgac cgtgtacag 
      caaattatt atttgtaat tgtgtgtat agtactcct 
      agttatgta aactgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY