Status
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Gene information
General information
TC011124 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4287538..4294497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011124
    iBeetle screen iB_06915

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 5 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    maxilla shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011124 RA
      MSEVQQQPM AAHVKRPKF VGCKDTTVP SVHGRPNNH 
      LNNKIRLSN HQRNLSLDF RSMGILLPP VAQITSSTS 
      PHLTLHHRN RSLDSALQR IPEVDVTPS PECEPTVVV 
      VVPPIVDPK TTRDELASL GSDDSGILC GSDSGSSDT 
      STTTRESSV DETKAECCI TPTTTLSQS NETSKGFGM 
      LRLLESKVF DVSMAMHYL FKSKEPGVQ SYIANKMFS 
      FDDGEVDFY LPQLVCMYI QMHDVAEVI HPYLIHRCR 
      KSIDFSLKC AWLLDAYSS DASVPSKKK PLGIKLRNL 
      ILSDELRPK DHKTPIMVS TTFSTLHST TTKKSHQRS 
      QSDASALGT RRPSTTDKR LCMGDLTSG RAFDNGCTC 
      FDSCRGVVN DLRGKKTEC TCSAPRLAP ELEFMKALI 
      SIGKLLSSI PTKEAKTTR LVAELTTLN LNLPARVWL 
      PLATTPHHI VRIPPQVGA VLNSKDKAP YIIYVEVVE 
      VDDLHTSIV TPKIWPTLR HTKSEENLG DTTSLSAFS 
      MCGGLCDDT DPDWSQEDD EISQQYTQL KRIADRDTL 
      SQMSQESSD SREPPMWIA AGEIRRRLS DSIHEEKNK 
      QFTHDPQDP SAAVLKEPW HDKERRIRE CSPYGHLTS 
      WTLLSVIVK CGDDLRQEL MASQLLQMF QKIWEMEHV 
      PLWVHPYRI LCLSNDSGL IEPILNTVS LHQIKKHRQ 
      LSLLEYFIN EYGPITCEE FLTAQKNFV QSCAAYCLF 
      CYLIQVKDR HNGNILLKS DGHLIHIDF GFILSMSPK 
      NLGFETSPF KLTAEFVEV MGGDQSDMF EYFKILILQ 
      GLVAARKHM DMIVPLVEI MRSGSQLPC FKSGAATVQ 
      NLKNRFHMS MTEEQLQLE VNRMVEVSI HSLSTKLYD 
      GFQYFTNGI L
    CDS sequence
    • >TC011124 RA
      atgagcgaa gtacaacaa caacccatg gcggcccac 
      gtaaaacgg ccaaaattc gttgggtgt aaagataca 
      acagtacca tcagtccat ggacgaccc aacaaccat 
      ctcaataac aagatcaga ctaagcaat catcaaagg 
      aatttgagt ttggacttt aggtcgatg ggtattttg 
      ctccctcct gtcgcccag ataacgtct tccacctcc 
      ccccacctc acccttcac catcgcaat cgcagtctt 
      gattcggcc cttcagcgt attcccgaa gtggacgtt 
      acaccaagt cccgaatgt gaaccgact gttgttgta 
      gttgtacca ccgatagtc gaccccaaa acaacacgt 
      gatgaactt gccagtttg gggtcggat gattcgggt 
      atattgtgt ggaagtgat tcgggttca agcgataca 
      agtacaaca acgcgtgaa tcaagcgtt gacgagacg 
      aaagccgag tgttgtata acaccaaca acaacacta 
      tcacaatcc aatgaaacg agtaaagga tttggaatg 
      ttgagatta ttggagagt aaagtgttt gatgtgtca 
      atggcaatg cattatctg tttaaaagt aaagaacca 
      ggtgtgcaa agttatata gcgaataag atgtttagt 
      tttgatgat ggagaagtg gatttttat ttgccacaa 
      ttggtttgt atgtatata caaatgcac gacgtggct 
      gaagttata catccgtat cttatacat aggtgtaga 
      aaatcgatc gacttttcg ctaaaatgt gcctggctt 
      ttggacgcc tatagttct gatgcttca gtaccatcg 
      aaaaagaaa ccactcggc atcaaactt cgtaatctt 
      atactttcc gacgaactt cgacccaaa gaccataaa 
      acacccatc atggtctct accaccttt agcactcta 
      cattcaaca actacgaaa aaatcacat caacgttca 
      caaagcgac gcttcagcc cttggtaca cgacgccct 
      tcaaccacc gacaaacgc ctctgtatg ggcgacctc 
      acctccggt cgtgccttt gacaacgga tgcacatgt 
      ttcgacagt tgcagaggc gtcgtcaat gacctacga 
      ggcaaaaag actgaatgc acgtgctcg gctcctaga 
      ctcgccccc gagcttgaa tttatgaaa gcgctgatt 
      tcaatcggt aaattactg tcttctata ccgaccaag 
      gaagcaaaa actacaaga ttagtagcg gaattaacg 
      acacttaat ttgaatttg ccggctcgt gtttggtta 
      cccctagca acgacccca catcacatt gttaggata 
      ccgccacaa gttggcgcc gtcttaaat tccaaagat 
      aaggcacct tatattatc tatgtcgaa gtggtggaa 
      gttgatgat ttgcatacg tcaattgtt acaccaaaa 
      atatggcca acgttacgt cacacaaaa tctgaggaa 
      aatttaggt gatacgacc tcattatcg gcgtttagt 
      atgtgtggg ggtttgtgt gatgatact gaccccgat 
      tggagtcag gaggatgat gaaatatcg caacagtat 
      acgcaattg aaacgtata gctgatcgc gatacgttg 
      tcgcaaatg tcacaagaa tcgagtgat tcgagggaa 
      cccccgatg tggatagca gccggggaa atacgcaga 
      cgtttgtct gattcaatt catgaggaa aaaaataaa 
      cagttcacc catgatcct caggatcct tctgccgca 
      gtgctgaag gaaccatgg catgataag gagagacgt 
      attcgtgaa tgtagccca tatgggcat ttgacatca 
      tggacgcta ctgtcagtg atagtgaaa tgtggcgat 
      gatttgcgt caggaatta atggcatcg caactttta 
      caaatgttc cagaaaata tgggagatg gaacatgta 
      ccattatgg gtgcaccca tatcgcata ctttgtcta 
      tcaaatgat agtgggttg attgaaccc attttgaat 
      actgtctca ctccatcaa atcaaaaaa catcgacaa 
      ttgtcactg ttggagtac tttatcaat gagtatgga 
      ccaataacg tgtgaggaa tttttgaca gctcagaaa 
      aatttcgta caaagttgt gccgcctat tgtctcttt 
      tgttatttg atacaagtg aaagatcga cataatgga 
      aatattttg ttgaaaagt gatggtcat ttaattcat 
      atcgatttc ggttttata ctgagtatg tcaccgaaa 
      aatttagga ttcgaaacg tcgccgttt aaattaacg 
      gctgagttt gttgaagtc atgggtggt gatcagtcg 
      gatatgttt gagtatttt aagatttta attttgcaa 
      ggattggtc gctgcaagg aaacatatg gatatgatt 
      gttccattg gtcgagatt atgagatca ggttcgcaa 
      ttgccgtgt tttaaatcg ggcgcagcg actgtccag 
      aatttgaag aaccgtttc cacatgagt atgaccgag 
      gagcagtta caactcgaa gtgaatcgt atggtggaa 
      gtcagtatt cactcacta tcaacgaaa ctttacgac 
      ggttttcaa tatttcaca aacgggata ctttaa
    mRNA sequence
    • >TC011124 RA
      acatggcgt ctttaccgt ggcagttgt gttgtgatt 
      gcgttaaaa atcccgttt tttgcactt aaaatcgag 
      ttatttccg gcggaaaag cgtcccggg accggaagt 
      ggcgtgcat gtgtgcaag tgcgccccc tttgaggat 
      attccagtg tttggtgta tgtgtgacg tttgtccgg 
      atttctcca caatttatt agaaagttt attcgcaaa 
      cgtctgtga taccgacaa tggaatgac gtattcgaa 
      atgagcgaa gtacaacaa caacccatg gcggcccac 
      gtaaaacgg ccaaaattc gttgggtgt aaagataca 
      acagtacca tcagtccat ggacgaccc aacaaccat 
      ctcaataac aagatcaga ctaagcaat catcaaagg 
      aatttgagt ttggacttt aggtcgatg ggtattttg 
      ctccctcct gtcgcccag ataacgtct tccacctcc 
      ccccacctc acccttcac catcgcaat cgcagtctt 
      gattcggcc cttcagcgt attcccgaa gtggacgtt 
      acaccaagt cccgaatgt gaaccgact gttgttgta 
      gttgtacca ccgatagtc gaccccaaa acaacacgt 
      gatgaactt gccagtttg gggtcggat gattcgggt 
      atattgtgt ggaagtgat tcgggttca agcgataca 
      agtacaaca acgcgtgaa tcaagcgtt gacgagacg 
      aaagccgag tgttgtata acaccaaca acaacacta 
      tcacaatcc aatgaaacg agtaaagga tttggaatg 
      ttgagatta ttggagagt aaagtgttt gatgtgtca 
      atggcaatg cattatctg tttaaaagt aaagaacca 
      ggtgtgcaa agttatata gcgaataag atgtttagt 
      tttgatgat ggagaagtg gatttttat ttgccacaa 
      ttggtttgt atgtatata caaatgcac gacgtggct 
      gaagttata catccgtat cttatacat aggtgtaga 
      aaatcgatc gacttttcg ctaaaatgt gcctggctt 
      ttggacgcc tatagttct gatgcttca gtaccatcg 
      aaaaagaaa ccactcggc atcaaactt cgtaatctt 
      atactttcc gacgaactt cgacccaaa gaccataaa 
      acacccatc atggtctct accaccttt agcactcta 
      cattcaaca actacgaaa aaatcacat caacgttca 
      caaagcgac gcttcagcc cttggtaca cgacgccct 
      tcaaccacc gacaaacgc ctctgtatg ggcgacctc 
      acctccggt cgtgccttt gacaacgga tgcacatgt 
      ttcgacagt tgcagaggc gtcgtcaat gacctacga 
      ggcaaaaag actgaatgc acgtgctcg gctcctaga 
      ctcgccccc gagcttgaa tttatgaaa gcgctgatt 
      tcaatcggt aaattactg tcttctata ccgaccaag 
      gaagcaaaa actacaaga ttagtagcg gaattaacg 
      acacttaat ttgaatttg ccggctcgt gtttggtta 
      cccctagca acgacccca catcacatt gttaggata 
      ccgccacaa gttggcgcc gtcttaaat tccaaagat 
      aaggcacct tatattatc tatgtcgaa gtggtggaa 
      gttgatgat ttgcatacg tcaattgtt acaccaaaa 
      atatggcca acgttacgt cacacaaaa tctgaggaa 
      aatttaggt gatacgacc tcattatcg gcgtttagt 
      atgtgtggg ggtttgtgt gatgatact gaccccgat 
      tggagtcag gaggatgat gaaatatcg caacagtat 
      acgcaattg aaacgtata gctgatcgc gatacgttg 
      tcgcaaatg tcacaagaa tcgagtgat tcgagggaa 
      cccccgatg tggatagca gccggggaa atacgcaga 
      cgtttgtct gattcaatt catgaggaa aaaaataaa 
      cagttcacc catgatcct caggatcct tctgccgca 
      gtgctgaag gaaccatgg catgataag gagagacgt 
      attcgtgaa tgtagccca tatgggcat ttgacatca 
      tggacgcta ctgtcagtg atagtgaaa tgtggcgat 
      gatttgcgt caggaatta atggcatcg caactttta 
      caaatgttc cagaaaata tgggagatg gaacatgta 
      ccattatgg gtgcaccca tatcgcata ctttgtcta 
      tcaaatgat agtgggttg attgaaccc attttgaat 
      actgtctca ctccatcaa atcaaaaaa catcgacaa 
      ttgtcactg ttggagtac tttatcaat gagtatgga 
      ccaataacg tgtgaggaa tttttgaca gctcagaaa 
      aatttcgta caaagttgt gccgcctat tgtctcttt 
      tgttatttg atacaagtg aaagatcga cataatgga 
      aatattttg ttgaaaagt gatggtcat ttaattcat 
      atcgatttc ggttttata ctgagtatg tcaccgaaa 
      aatttagga ttcgaaacg tcgccgttt aaattaacg 
      gctgagttt gttgaagtc atgggtggt gatcagtcg 
      gatatgttt gagtatttt aagatttta attttgcaa 
      ggattggtc gctgcaagg aaacatatg gatatgatt 
      gttccattg gtcgagatt atgagatca ggttcgcaa 
      ttgccgtgt tttaaatcg ggcgcagcg actgtccag 
      aatttgaag aaccgtttc cacatgagt atgaccgag 
      gagcagtta caactcgaa gtgaatcgt atggtggaa 
      gtcagtatt cactcacta tcaacgaaa ctttacgac 
      ggttttcaa tatttcaca aacgggata ctttaaaaa 
      aatgctcaa ttatatcat aaacaccat gtataataa 
      ttttagtgc gaaataaag tgatctgat ttgccatgt 
      gagtgaaga atactcagc ggccaaagt gtggaagca 
      aaaaaactt tgtatatat aacattcca tttgtacag 
      ttgtttttt aaaaatcta gtgtttttt gtaaatatc 
      tacgtaaag ttttagtgt tggctagat aattcacgt 
      tatccatcc atttttaaa taaaagtaa tatataagt 
      atatattgt ataaataca tgactgaaa ttatataaa 
      tgaaatgca aaaagactg atgtcagca gtaggcttt 
      ttgcaggtg ttattactt tttttttct tgagttgga 
      aaccaatca aaactgtgc atgttttat tatgaaatt 
      tttgaatat tgtatttta ttatattat ctatgaatt 
      gtatttcaa ataaacgat aaattaata a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY