Status
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Gene information
General information
TC003397 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4236004..4255109
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003397
    iBeetle screen iB_06914

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003397 RA
      MLIKEYRIP LPLTVEEYR IAQLYMIAK KSREESSGA 
      GSGVEILVN EPYKDGPGG QGQYTRKIY HVGSHLPGW 
      LKGILPKTA LMVEEEAWN AYPYTKTRY TCPFVEKFS 
      LEIETYYYQ DNGHQENVF NLTGSDLRN RIVDVIDVV 
      KDQLYGADY VKNEDPVYF KSEKSGRGP LSENWLEEY 
      WREVQGKQQ PLPNGKSLM CAYKLCRVE FRYWGMQTK 
      IEKFIHDIA LRKTMLRAH RQAWAWQDE WHGLTMEDI 
      REIEKQTQL ALKRKMGQV EDEEIDEDD NLNNSTPKE 
      DPVKTLAAT LGSIEVSED SPLMTKNSN IPSIQTAIS 
      SDGDLSPED ETGVHCKRE EKWQQRGLH SPSSSSIKS 
      FDLQVANWR IESLGRDSD SGSDEEFFD CEDSSSLAK 
      WSSLDLLTE EDADTTSPT VVVDKNDDS IFSPTFLQR 
      VASERSNRK MMQRGKVNS LDASCPESP TASPAHLPC 
      NTTVLLLVL HAGSVLDAN IDMAAKKSD VTTLRGAFE 
      SVMRQHYPS LLGHVAIRL VSCPSICTE GLAILSSLS 
      PYSFDVSPS CIDVPQITH DSIPIGAIP ILASSTSDY 
      QEAVSRTIN AANTTYQEF LKSEEGQGF AGQVCIVAD 
      SIGSILAYD ALCRSTKYQ SRHGSENSI LDNDNYHRD 
      EVQINETGY LTAPSPRRR SSSTSDQSN QMKFEFDTT 
      DVFMFGSPL ALVLAYRKI AATDEKSNT ITRPSCVQV 
      YNLFHPTDP VAARLEPLL SARFSILPP VNVARYAKY 
      PLGNGQPYH LLEILQTNP QMFSDSSQH RRLSEVSIQ 
      STVSGLIDN IPLQAINAL QQKWWGGKR MDYALYCPE 
      GLSNFPTNA LPHLFHASY WESCDVIAF ILRQVGGIE 
      AAPLSGVED RELCFKPGQ PREKWMKKR TSVKVKNVT 
      ANHRANDII VREGAPQVL SARFMYGPL DMVALTGEK 
      VDIHVMKEV AVGEWVQIA TEVTDKAGR ITHTIPEEK 
      TLGYGLYPI KMVVRGDHT SVDFFMAVV PPKTECVVF 
      SIDGSFTAS MSVTGRDPK VRAGAVDVV RHWQELGYL 
      IVYITGRPD MQHRRVVSW LSQHNFPHG LVSFADGLS 
      TDPLGHKAA YLNNLIQNH GVIIHCAYG SSKDISVYT 
      SVGLKPKQI FIVGKASKK QHAQATIRV DGYAAHLTM 
      LMAHGGSRP AQGNARMVL PRGYFGLPG QSFNRRRSR 
      SAKRATSYP IATEVVTPL ERSLSSRRY QLPKPTLST 
      IGNSSG
    • >TC003397 RB
      MLIKEYRIP LPLTVEEYR IAQLYMIAK KSREESSGA 
      GSGVEILVN EPYKDGPGG QGQYTRKIY HVGSHLPGW 
      LKGILPKTA LMVEEEAWN AYPYTKTRY TCPFVEKFS 
      LEIETYYYQ DNGHQENVF NLTGSDLRN RIVDVIDVV 
      KDQLYGADY VKNEDPVYF KSEKSGRGP LSENWLEEY 
      WREVQGKQQ PLPNGKSLM CAYKLCRVE FRYWGMQTK 
      IEKFIHDIA LRKTMLRAH RQAWAWQDE WHGLTMEDI 
      REIEKQTQL ALKRKMGQV EDEEIDEDD NLNNSTPKE 
      DPVKTLAAT LGSIEVSED SPLMTKNSN IPSIQTAIS 
      SDGDLSPED ETGVHCKRE EKWQQRGLH SPSSSSIKS 
      FDLQVANWR IESLGRDSD SGSDEEFFD CEDSSSLAK 
      WSSLDLLTE EDADTTSPT VVVDKNDDS IFSPTFLQR 
      VASERSNRK MMQRGKVNS LDASCPESP TASPAHLPC 
      NTTVLLLVL HAGSVLDAN IDMAAKKSD VTTLRGAFE 
      SVMRQHYPS LLGHVAIRL VSCPSICTE GLAILSSLS 
      PYSFDVSPS CIDVPQITH DSIPIGAIP ILASSTSDY 
      QEAVSRTIN AANTTYQEF LKSEEGQGF AGQVCIVAD 
      SIGSILAYD ALCRSTKYQ SRHGSENSI LDNDNYHRD 
      EVQINETGY LTAPSPRRR SSSTSDQSN QMKFEFDTT 
      DVFMFGSPL ALVLAYRKI AATDEKSNT ITRPSCVQV 
      YNLFHPTDP VAARLEPLL SARFSILPP VNVARYAKY 
      PLGNGQPYH LLEILQTNP QMFSDSSQH RRLSEVSIQ 
      STVSGLIDN IPLQAINAL QQKWWGGKR MDYALYCPE 
      GLSNFPTNA LPHLFHASY WESCDVIAF ILRQVGGIE 
      AAPLSGVED RELCFKPGQ PREKWMKKR TSVKVKNVT 
      ANHRANDII VREGAPQVL SARFMYGPL DMVALTGEK 
      VDIHVMKEV AVGEWVQIA TEVTDKAGR ITHTIPEEK 
      TLGYGLYPI KMVVRGDHT SVDFFMAVV PPKTECVVF 
      SIDGSFTAS MSVTGRDPK VRAGAVDVV RHWQELGYL 
      IVYITGRPD MQHRRVVSW LSQHNFPHG LVSFADGLS 
      TDPLGHKAA YLNNLIQNH GVIIHCAYG SSKDISVYT 
      SVGLKPKQI FIVGKASKK QHAQATIRV DGYAAHLTM 
      LMAHGGSRP AQGNARMVL PRGYFGLPG QSFNRRRR
    CDS sequence
    • >TC003397 RA
      atgctcatc aaggaatat cgtattcct ctgcctctc 
      acggtggag gaatatcga attgctcag ctgtatatg 
      attgctaaa aaaagtcgc gaggagagc agcggggca 
      ggcagtggt gtcgaaatt ctggtcaat gagccttac 
      aaagatggg cctggcgga caaggacag tacacccgt 
      aagatatat catgtgggg agccattta ccaggatgg 
      ctgaagggc atcctcccg aaaaccgcc ctaatggtg 
      gaagaggag gcgtggaac gcgtatcca tacaccaag 
      acgcggtac acatgtccc tttgtcgaa aagttctcc 
      ctcgagatc gagacctat tattaccag gataacggg 
      caccaggag aatgtgttt aatttaact gggagtgat 
      ctaaggaac aggatcgtc gatgtgata gatgtagtg 
      aaggatcag ttgtacggt gctgactac gtcaaaaat 
      gaggatccg gtgtatttc aagtcggaa aagtccggc 
      agggggccg ttatcggaa aattggctc gaggagtat 
      tggagggag gttcaggga aaacaacaa ccgttacct 
      aatggaaag tctctaatg tgcgcttat aaattatgc 
      agagtggag tttaggtat tgggggatg cagacgaaa 
      atcgagaaa tttattcac gatatagcg cttcggaaa 
      acaatgctg agggctcat agacaggcg tgggcttgg 
      caagacgaa tggcacggt ttgactatg gaggatatt 
      agggaaata gaaaaacag acgcagctt gcgctgaag 
      aggaaaatg gggcaggtg gaggacgag gaaattgac 
      gaagatgat aatctgaat aacagtaca cctaaagaa 
      gacccggta aaaacccta gctgcgacc ctcggcagc 
      atagaagta tcagaagac agtccccta atgactaaa 
      aattcaaac attccttca atccagacc gcaatcagt 
      tccgacggt gacttatca ccggaagac gaaacagga 
      gtgcattgc aaacgcgag gagaaatgg caacagcga 
      ggtttgcac tccccgagc tcttcatcg atcaaaagc 
      ttcgatttg caagtggcg aattggagg attgaaagt 
      ctgggacgc gactcggat tccgggtcg gacgaagag 
      tttttcgac tgtgaagat tcgtcctcg ttggccaag 
      tggagctcg ttggacttg ctgaccgag gaggacgct 
      gatactacc tcaccgacg gtcgtggtt gacaaaaac 
      gacgacagc atattctcc cctacattc ctgcaacgc 
      gtggcgagc gaacgcagc aaccgcaag atgatgcag 
      agaggcaaa gtgaactcg cttgatgcc tcatgcccg 
      gagtctccg actgcgtcc cccgcacat cttccatgc 
      aataccaca gtattattg ttagtgctt cacgctggg 
      agtgtccta gatgccaac atcgacatg gcggcgaag 
      aagtcggac gtgaccacc ttgcgtggg gctttcgag 
      tcggttatg cgccagcat tatccgtcg ctgttaggc 
      catgttgcg attcgattg gtgtcttgt ccctctatc 
      tgtactgaa ggccttgca atcctgtct agtcttagt 
      ccgtatagt ttcgatgtt tcgccttcg tgtatcgat 
      gtacctcaa atcacacac gactccatt ccgattggt 
      gctattcca atcctagcc agttccacc tctgactat 
      caagaagcg gtttcacgg acaatcaat gcagcaaat 
      accacttat caagagttc ttaaagtca gaggagggc 
      cagggtttc gccggtcaa gtgtgcatc gttgcggat 
      tcaattggt tcaatatta gcttatgac gctttatgt 
      agatcgacg aaataccag tcaaggcat ggcagtgag 
      aacagtatt ttagacaat gataattac cacagagac 
      gaggttcaa atcaacgaa actgggtac ttgacggca 
      ccctctcct cgcagaaga tcgtcctca accagcgat 
      caatccaat cagatgaag ttcgagttc gatacgacc 
      gatgtgttt atgtttggt agtccttta gcgttagtt 
      ctagcctat agaaaaatt gcagcaact gatgaaaaa 
      tccaatacc ataacccgt ccaagttgc gtgcaagtt 
      tacaatttg ttccatccc actgatcca gtagctgca 
      cgtttggag ccacttttg tcggcacgg ttttcgatt 
      ctgccgccg gtgaatgtg gcaagatat gcgaagtat 
      cccctggga aacgggcaa ccgtatcac ttgctcgaa 
      attcttcaa actaatcct cagatgttt agtgattct 
      tcacaacat agacggtta tctgaggtt tctatacaa 
      agtacagtt tctggatta attgataat atacccctt 
      caagctatt aatgctttg caacaaaaa tggtggggt 
      ggcaaacga atggattac gcgttatat tgccctgaa 
      ggcttgtca aacttcccc accaacgcc ctgccacac 
      ttattccac gcaagttac tgggaaagt tgcgacgtg 
      atcgctttt attttgcgc caagttggc ggtattgaa 
      gctgcacct ttgtcaggt gttgaggat agggaacta 
      tgtttcaaa cctggacaa cctcgggag aaatggatg 
      aaaaagcga acgtcggtc aaagtgaaa aatgtcaca 
      gctaatcat cgggcgaat gatataatc gtgcgagaa 
      ggcgcacct caggtgtta tcagccagg tttatgtac 
      gggccccta gatatggtc gcattgacc ggcgaaaaa 
      gtcgatatt catgtaatg aaggaagtg gcggtggga 
      gaatgggtt cagatagct acagaggtg acggataaa 
      gccgggaga attactcat acaattccg gaagaaaaa 
      acattgggt tacggttta tatcctata aagatggtg 
      gtaaggggt gatcacacc tctgtcgat ttttttatg 
      gctgtagtt cctccaaaa accgaatgt gtggtgttt 
      agtattgat gggtctttt actgccagt atgtcagtg 
      acgggaagg gatccgaag gttagagcg ggggccgtt 
      gatgtcgtt agacattgg caagagctt ggttacctc 
      atcgtttac ataactgga agacctgac atgcagcac 
      cgaagagtt gtgtcatgg ttatcccaa cataatttc 
      cctcatggt ctcgtgtca tttgctgat ggtctttca 
      accgatcct ttaggacat aaggctgct tatctcaat 
      aacttaatc caaaatcat ggtgtaatt atccattgt 
      gcatacggt agcagtaaa gacataagt gtatatacc 
      tcagtcgga ctgaaacct aaacaaatc ttcatcgtc 
      ggcaaagcg tcaaagaaa caacatgct caagcaaca 
      atccgagta gacggctat gccgcccat ctcaccatg 
      ctgatggcc catgggggc tcccgacct gcgcaagga 
      aacgcccga atggttcta ccacgcggc tatttcggt 
      ctgccaggc caaagtttt aaccgaagg agaagccgt 
      tccgccaaa agagccaca tcttatccg atcgccacc 
      gaggtcgtt actccactg gaaagatca ctaagttct 
      cgtcggtat caattgcca aaacctacg ctttcaact 
      attggaaat agttccgga taa
    • >TC003397 RB
      atgctcatc aaggaatat cgtattcct ctgcctctc 
      acggtggag gaatatcga attgctcag ctgtatatg 
      attgctaaa aaaagtcgc gaggagagc agcggggca 
      ggcagtggt gtcgaaatt ctggtcaat gagccttac 
      aaagatggg cctggcgga caaggacag tacacccgt 
      aagatatat catgtgggg agccattta ccaggatgg 
      ctgaagggc atcctcccg aaaaccgcc ctaatggtg 
      gaagaggag gcgtggaac gcgtatcca tacaccaag 
      acgcggtac acatgtccc tttgtcgaa aagttctcc 
      ctcgagatc gagacctat tattaccag gataacggg 
      caccaggag aatgtgttt aatttaact gggagtgat 
      ctaaggaac aggatcgtc gatgtgata gatgtagtg 
      aaggatcag ttgtacggt gctgactac gtcaaaaat 
      gaggatccg gtgtatttc aagtcggaa aagtccggc 
      agggggccg ttatcggaa aattggctc gaggagtat 
      tggagggag gttcaggga aaacaacaa ccgttacct 
      aatggaaag tctctaatg tgcgcttat aaattatgc 
      agagtggag tttaggtat tgggggatg cagacgaaa 
      atcgagaaa tttattcac gatatagcg cttcggaaa 
      acaatgctg agggctcat agacaggcg tgggcttgg 
      caagacgaa tggcacggt ttgactatg gaggatatt 
      agggaaata gaaaaacag acgcagctt gcgctgaag 
      aggaaaatg gggcaggtg gaggacgag gaaattgac 
      gaagatgat aatctgaat aacagtaca cctaaagaa 
      gacccggta aaaacccta gctgcgacc ctcggcagc 
      atagaagta tcagaagac agtccccta atgactaaa 
      aattcaaac attccttca atccagacc gcaatcagt 
      tccgacggt gacttatca ccggaagac gaaacagga 
      gtgcattgc aaacgcgag gagaaatgg caacagcga 
      ggtttgcac tccccgagc tcttcatcg atcaaaagc 
      ttcgatttg caagtggcg aattggagg attgaaagt 
      ctgggacgc gactcggat tccgggtcg gacgaagag 
      tttttcgac tgtgaagat tcgtcctcg ttggccaag 
      tggagctcg ttggacttg ctgaccgag gaggacgct 
      gatactacc tcaccgacg gtcgtggtt gacaaaaac 
      gacgacagc atattctcc cctacattc ctgcaacgc 
      gtggcgagc gaacgcagc aaccgcaag atgatgcag 
      agaggcaaa gtgaactcg cttgatgcc tcatgcccg 
      gagtctccg actgcgtcc cccgcacat cttccatgc 
      aataccaca gtattattg ttagtgctt cacgctggg 
      agtgtccta gatgccaac atcgacatg gcggcgaag 
      aagtcggac gtgaccacc ttgcgtggg gctttcgag 
      tcggttatg cgccagcat tatccgtcg ctgttaggc 
      catgttgcg attcgattg gtgtcttgt ccctctatc 
      tgtactgaa ggccttgca atcctgtct agtcttagt 
      ccgtatagt ttcgatgtt tcgccttcg tgtatcgat 
      gtacctcaa atcacacac gactccatt ccgattggt 
      gctattcca atcctagcc agttccacc tctgactat 
      caagaagcg gtttcacgg acaatcaat gcagcaaat 
      accacttat caagagttc ttaaagtca gaggagggc 
      cagggtttc gccggtcaa gtgtgcatc gttgcggat 
      tcaattggt tcaatatta gcttatgac gctttatgt 
      agatcgacg aaataccag tcaaggcat ggcagtgag 
      aacagtatt ttagacaat gataattac cacagagac 
      gaggttcaa atcaacgaa actgggtac ttgacggca 
      ccctctcct cgcagaaga tcgtcctca accagcgat 
      caatccaat cagatgaag ttcgagttc gatacgacc 
      gatgtgttt atgtttggt agtccttta gcgttagtt 
      ctagcctat agaaaaatt gcagcaact gatgaaaaa 
      tccaatacc ataacccgt ccaagttgc gtgcaagtt 
      tacaatttg ttccatccc actgatcca gtagctgca 
      cgtttggag ccacttttg tcggcacgg ttttcgatt 
      ctgccgccg gtgaatgtg gcaagatat gcgaagtat 
      cccctggga aacgggcaa ccgtatcac ttgctcgaa 
      attcttcaa actaatcct cagatgttt agtgattct 
      tcacaacat agacggtta tctgaggtt tctatacaa 
      agtacagtt tctggatta attgataat atacccctt 
      caagctatt aatgctttg caacaaaaa tggtggggt 
      ggcaaacga atggattac gcgttatat tgccctgaa 
      ggcttgtca aacttcccc accaacgcc ctgccacac 
      ttattccac gcaagttac tgggaaagt tgcgacgtg 
      atcgctttt attttgcgc caagttggc ggtattgaa 
      gctgcacct ttgtcaggt gttgaggat agggaacta 
      tgtttcaaa cctggacaa cctcgggag aaatggatg 
      aaaaagcga acgtcggtc aaagtgaaa aatgtcaca 
      gctaatcat cgggcgaat gatataatc gtgcgagaa 
      ggcgcacct caggtgtta tcagccagg tttatgtac 
      gggccccta gatatggtc gcattgacc ggcgaaaaa 
      gtcgatatt catgtaatg aaggaagtg gcggtggga 
      gaatgggtt cagatagct acagaggtg acggataaa 
      gccgggaga attactcat acaattccg gaagaaaaa 
      acattgggt tacggttta tatcctata aagatggtg 
      gtaaggggt gatcacacc tctgtcgat ttttttatg 
      gctgtagtt cctccaaaa accgaatgt gtggtgttt 
      agtattgat gggtctttt actgccagt atgtcagtg 
      acgggaagg gatccgaag gttagagcg ggggccgtt 
      gatgtcgtt agacattgg caagagctt ggttacctc 
      atcgtttac ataactgga agacctgac atgcagcac 
      cgaagagtt gtgtcatgg ttatcccaa cataatttc 
      cctcatggt ctcgtgtca tttgctgat ggtctttca 
      accgatcct ttaggacat aaggctgct tatctcaat 
      aacttaatc caaaatcat ggtgtaatt atccattgt 
      gcatacggt agcagtaaa gacataagt gtatatacc 
      tcagtcgga ctgaaacct aaacaaatc ttcatcgtc 
      ggcaaagcg tcaaagaaa caacatgct caagcaaca 
      atccgagta gacggctat gccgcccat ctcaccatg 
      ctgatggcc catgggggc tcccgacct gcgcaagga 
      aacgcccga atggttcta ccacgcggc tatttcggt 
      ctgccaggc caaagtttt aaccgaagg agaaggtaa
    mRNA sequence
    • >TC003397 RA
      attatcaaa cacataata ggcataata gtaaattat 
      ttgcctttt gttaaactc gacacaact ttacgtaat 
      gtattttag ttgaagtag tgctgcgag tgcccggtt 
      gtataacca gttgagtca agtctcaca gaaatgagc 
      tttttgggc ccaaaatga attaactag aataatgtg 
      gataacttg ggatccatt ccaaatcgt ctaaagtgg 
      acggacgtc ctcgtcagc catgctcat caaggaata 
      tcgtattcc tctgcctct cacggtgga ggaatatcg 
      aattgctca gctgtatat gattgctaa aaaaagtcg 
      cgaggagag cagcggggc aggcagtgg tgtcgaaat 
      tctggtcaa tgagcctta caaagatgg gcctggcgg 
      acaaggaca gtacacccg taagatata tcatgtggg 
      gagccattt accaggatg gctgaaggg catcctccc 
      gaaaaccgc cctaatggt ggaagagga ggcgtggaa 
      cgcgtatcc atacaccaa gacgcggta cacatgtcc 
      ctttgtcga aaagttctc cctcgagat cgagaccta 
      ttattacca ggataacgg gcaccagga gaatgtgtt 
      taatttaac tgggagtga tctaaggaa caggatcgt 
      cgatgtgat agatgtagt gaaggatca gttgtacgg 
      tgctgacta cgtcaaaaa tgaggatcc ggtgtattt 
      caagtcgga aaagtccgg cagggggcc gttatcgga 
      aaattggct cgaggagta ttggaggga ggttcaggg 
      aaaacaaca accgttacc taatggaaa gtctctaat 
      gtgcgctta taaattatg cagagtgga gtttaggta 
      ttgggggat gcagacgaa aatcgagaa atttattca 
      cgatatagc gcttcggaa aacaatgct gagggctca 
      tagacaggc gtgggcttg gcaagacga atggcacgg 
      tttgactat ggaggatat tagggaaat agaaaaaca 
      gacgcagct tgcgctgaa gaggaaaat ggggcaggt 
      ggaggacga ggaaattga cgaagatga taatctgaa 
      taacagtac acctaaaga agacccggt aaaaaccct 
      agctgcgac cctcggcag catagaagt atcagaaga 
      cagtcccct aatgactaa aaattcaaa cattccttc 
      aatccagac cgcaatcag ttccgacgg tgacttatc 
      accggaaga cgaaacagg agtgcattg caaacgcga 
      ggagaaatg gcaacagcg aggtttgca ctccccgag 
      ctcttcatc gatcaaaag cttcgattt gcaagtggc 
      gaattggag gattgaaag tctgggacg cgactcgga 
      ttccgggtc ggacgaaga gtttttcga ctgtgaaga 
      ttcgtcctc gttggccaa gtggagctc gttggactt 
      gctgaccga ggaggacgc tgatactac ctcaccgac 
      ggtcgtggt tgacaaaaa cgacgacag catattctc 
      ccctacatt cctgcaacg cgtggcgag cgaacgcag 
      caaccgcaa gatgatgca gagaggcaa agtgaactc 
      gcttgatgc ctcatgccc ggagtctcc gactgcgtc 
      ccccgcaca tcttccatg caataccac agtattatt 
      gttagtgct tcacgctgg gagtgtcct agatgccaa 
      catcgacat ggcggcgaa gaagtcgga cgtgaccac 
      cttgcgtgg ggctttcga gtcggttat gcgccagca 
      ttatccgtc gctgttagg ccatgttgc gattcgatt 
      ggtgtcttg tccctctat ctgtactga aggccttgc 
      aatcctgtc tagtcttag tccgtatag tttcgatgt 
      ttcgccttc gtgtatcga tgtacctca aatcacaca 
      cgactccat tccgattgg tgctattcc aatcctagc 
      cagttccac ctctgacta tcaagaagc ggtttcacg 
      gacaatcaa tgcagcaaa taccactta tcaagagtt 
      cttaaagtc agaggaggg ccagggttt cgccggtca 
      agtgtgcat cgttgcgga ttcaattgg ttcaatatt 
      agcttatga cgctttatg tagatcgac gaaatacca 
      gtcaaggca tggcagtga gaacagtat tttagacaa 
      tgataatta ccacagaga cgaggttca aatcaacga 
      aactgggta cttgacggc accctctcc tcgcagaag 
      atcgtcctc aaccagcga tcaatccaa tcagatgaa 
      gttcgagtt cgatacgac cgatgtgtt tatgtttgg 
      tagtccttt agcgttagt tctagccta tagaaaaat 
      tgcagcaac tgatgaaaa atccaatac cataacccg 
      tccaagttg cgtgcaagt ttacaattt gttccatcc 
      cactgatcc agtagctgc acgtttgga gccactttt 
      gtcggcacg gttttcgat tctgccgcc ggtgaatgt 
      ggcaagata tgcgaagta tcccctggg aaacgggca 
      accgtatca cttgctcga aattcttca aactaatcc 
      tcagatgtt tagtgattc ttcacaaca tagacggtt 
      atctgaggt ttctataca aagtacagt ttctggatt 
      aattgataa tatacccct tcaagctat taatgcttt 
      gcaacaaaa atggtgggg tggcaaacg aatggatta 
      cgcgttata ttgccctga aggcttgtc aaacttccc 
      caccaacgc cctgccaca cttattcca cgcaagtta 
      ctgggaaag ttgcgacgt gatcgcttt tattttgcg 
      ccaagttgg cggtattga agctgcacc tttgtcagg 
      tgttgagga tagggaact atgtttcaa acctggaca 
      acctcggga gaaatggat gaaaaagcg aacgtcggt 
      caaagtgaa aaatgtcac agctaatca tcgggcgaa 
      tgatataat cgtgcgaga aggcgcacc tcaggtgtt 
      atcagccag gtttatgta cgggcccct agatatggt 
      cgcattgac cggcgaaaa agtcgatat tcatgtaat 
      gaaggaagt ggcggtggg agaatgggt tcagatagc 
      tacagaggt gacggataa agccgggag aattactca 
      tacaattcc ggaagaaaa aacattggg ttacggttt 
      atatcctat aaagatggt ggtaagggg tgatcacac 
      ctctgtcga tttttttat ggctgtagt tcctccaaa 
      aaccgaatg tgtggtgtt tagtattga tgggtcttt 
      tactgccag tatgtcagt gacgggaag ggatccgaa 
      ggttagagc gggggccgt tgatgtcgt tagacattg 
      gcaagagct tggttacct catcgttta cataactgg 
      aagacctga catgcagca ccgaagagt tgtgtcatg 
      gttatccca acataattt ccctcatgg tctcgtgtc 
      atttgctga tggtctttc aaccgatcc tttaggaca 
      taaggctgc ttatctcaa taacttaat ccaaaatca 
      tggtgtaat tatccattg tgcatacgg tagcagtaa 
      agacataag tgtatatac ctcagtcgg actgaaacc 
      taaacaaat cttcatcgt cggcaaagc gtcaaagaa 
      acaacatgc tcaagcaac aatccgagt agacggcta 
      tgccgccca tctcaccat gctgatggc ccatggggg 
      ctcccgacc tgcgcaagg aaacgcccg aatggttct 
      accacgcgg ctatttcgg tctgccagg ccaaagttt 
      taaccgaag gagaagccg ttccgccaa aagagccac 
      atcttatcc gatcgccac cgaggtcgt tactccact 
      ggaaagatc actaagttc tcgtcggta tcaattgcc 
      aaaacctac gctttcaac tattggaaa tagttccgg 
      ataagtagc tcccagcga gcttgaaaa tatacatag 
      tactataaa aaaacactt ttagatctt gccattgtt 
      tatattttt ctaccttta acacagaaa aaatatact 
      atgattgaa aaagtgttg ttgtcggct gttacactg 
      aaacaaagt attttttgt aacattgcg tttctgcta 
      ttgttgcat acaatgaac aagtaaaag gaacaaact 
      agttattag tgttggcag ttttttctt tgtgtactt 
      acttacata tcttttaag tgattttct ttacgtaac 
      ttgaaaaat gtgattgta cactgttcc agaaagcgt 
      aatatgtac ttaaaacta agggataat tagacaatt 
      aaataagat atatctaaa gatgcttgt tcattgtta 
      taaatgtgt tgtgtaaat aactattac tgtaaaaat 
      atagataga aacataata aaaactata ttttgaaag 
      agtaacaaa tatttttat ttaggacac gtcgaaaaa 
      gagatattc atatttttc ttatttttc aagtttgta 
      gtcatagga cacgaagtt ggtttattc catactgat 
      gttacgctt tccaaaaat attgtaatt ttaactgtt 
      gatcaaaat attatgtac aattttttc gttaccaaa 
      gtttgcttt tatatctct cttgttatg ttacaataa 
      caataaatg tgacgaaat tcctgttgt tctgtagat 
      actccattt gagacatat catttaaaa aaatacacg 
      aaagtacct gttgtaggc caaataatc taaattgga 
      ggtcactct ctttaaata atcgaaaaa tgagcaata 
      gacaactaa atgaagtat ctacacatt gtgtatgaa 
      ttcctgcaa gttattatg gttaggtgc gtgacatgt 
      ttggtacct caaatcgcc gttaattgg tagggatgt 
      tttttgagt tacaacaaa taagtttat tgaatttat 
      ctgaaaata agtgtagag atgaataaa actaaaaat 
      aagca
    • >TC003397 RB
      attatcaaa cacataata ggcataata gtaaattat 
      ttgcctttt gttaaactc gacacaact ttacgtaat 
      gtattttag ttgaagtag tgctgcgag tgcccggtt 
      gtataacca gttgagtca agtctcaca gaaatgagc 
      tttttgggc ccaaaatga attaactag aataatgtg 
      gataacttg ggatccatt ccaaatcgt ctaaagtgg 
      acggacgtc ctcgtcagc catgctcat caaggaata 
      tcgtattcc tctgcctct cacggtgga ggaatatcg 
      aattgctca gctgtatat gattgctaa aaaaagtcg 
      cgaggagag cagcggggc aggcagtgg tgtcgaaat 
      tctggtcaa tgagcctta caaagatgg gcctggcgg 
      acaaggaca gtacacccg taagatata tcatgtggg 
      gagccattt accaggatg gctgaaggg catcctccc 
      gaaaaccgc cctaatggt ggaagagga ggcgtggaa 
      cgcgtatcc atacaccaa gacgcggta cacatgtcc 
      ctttgtcga aaagttctc cctcgagat cgagaccta 
      ttattacca ggataacgg gcaccagga gaatgtgtt 
      taatttaac tgggagtga tctaaggaa caggatcgt 
      cgatgtgat agatgtagt gaaggatca gttgtacgg 
      tgctgacta cgtcaaaaa tgaggatcc ggtgtattt 
      caagtcgga aaagtccgg cagggggcc gttatcgga 
      aaattggct cgaggagta ttggaggga ggttcaggg 
      aaaacaaca accgttacc taatggaaa gtctctaat 
      gtgcgctta taaattatg cagagtgga gtttaggta 
      ttgggggat gcagacgaa aatcgagaa atttattca 
      cgatatagc gcttcggaa aacaatgct gagggctca 
      tagacaggc gtgggcttg gcaagacga atggcacgg 
      tttgactat ggaggatat tagggaaat agaaaaaca 
      gacgcagct tgcgctgaa gaggaaaat ggggcaggt 
      ggaggacga ggaaattga cgaagatga taatctgaa 
      taacagtac acctaaaga agacccggt aaaaaccct 
      agctgcgac cctcggcag catagaagt atcagaaga 
      cagtcccct aatgactaa aaattcaaa cattccttc 
      aatccagac cgcaatcag ttccgacgg tgacttatc 
      accggaaga cgaaacagg agtgcattg caaacgcga 
      ggagaaatg gcaacagcg aggtttgca ctccccgag 
      ctcttcatc gatcaaaag cttcgattt gcaagtggc 
      gaattggag gattgaaag tctgggacg cgactcgga 
      ttccgggtc ggacgaaga gtttttcga ctgtgaaga 
      ttcgtcctc gttggccaa gtggagctc gttggactt 
      gctgaccga ggaggacgc tgatactac ctcaccgac 
      ggtcgtggt tgacaaaaa cgacgacag catattctc 
      ccctacatt cctgcaacg cgtggcgag cgaacgcag 
      caaccgcaa gatgatgca gagaggcaa agtgaactc 
      gcttgatgc ctcatgccc ggagtctcc gactgcgtc 
      ccccgcaca tcttccatg caataccac agtattatt 
      gttagtgct tcacgctgg gagtgtcct agatgccaa 
      catcgacat ggcggcgaa gaagtcgga cgtgaccac 
      cttgcgtgg ggctttcga gtcggttat gcgccagca 
      ttatccgtc gctgttagg ccatgttgc gattcgatt 
      ggtgtcttg tccctctat ctgtactga aggccttgc 
      aatcctgtc tagtcttag tccgtatag tttcgatgt 
      ttcgccttc gtgtatcga tgtacctca aatcacaca 
      cgactccat tccgattgg tgctattcc aatcctagc 
      cagttccac ctctgacta tcaagaagc ggtttcacg 
      gacaatcaa tgcagcaaa taccactta tcaagagtt 
      cttaaagtc agaggaggg ccagggttt cgccggtca 
      agtgtgcat cgttgcgga ttcaattgg ttcaatatt 
      agcttatga cgctttatg tagatcgac gaaatacca 
      gtcaaggca tggcagtga gaacagtat tttagacaa 
      tgataatta ccacagaga cgaggttca aatcaacga 
      aactgggta cttgacggc accctctcc tcgcagaag 
      atcgtcctc aaccagcga tcaatccaa tcagatgaa 
      gttcgagtt cgatacgac cgatgtgtt tatgtttgg 
      tagtccttt agcgttagt tctagccta tagaaaaat 
      tgcagcaac tgatgaaaa atccaatac cataacccg 
      tccaagttg cgtgcaagt ttacaattt gttccatcc 
      cactgatcc agtagctgc acgtttgga gccactttt 
      gtcggcacg gttttcgat tctgccgcc ggtgaatgt 
      ggcaagata tgcgaagta tcccctggg aaacgggca 
      accgtatca cttgctcga aattcttca aactaatcc 
      tcagatgtt tagtgattc ttcacaaca tagacggtt 
      atctgaggt ttctataca aagtacagt ttctggatt 
      aattgataa tatacccct tcaagctat taatgcttt 
      gcaacaaaa atggtgggg tggcaaacg aatggatta 
      cgcgttata ttgccctga aggcttgtc aaacttccc 
      caccaacgc cctgccaca cttattcca cgcaagtta 
      ctgggaaag ttgcgacgt gatcgcttt tattttgcg 
      ccaagttgg cggtattga agctgcacc tttgtcagg 
      tgttgagga tagggaact atgtttcaa acctggaca 
      acctcggga gaaatggat gaaaaagcg aacgtcggt 
      caaagtgaa aaatgtcac agctaatca tcgggcgaa 
      tgatataat cgtgcgaga aggcgcacc tcaggtgtt 
      atcagccag gtttatgta cgggcccct agatatggt 
      cgcattgac cggcgaaaa agtcgatat tcatgtaat 
      gaaggaagt ggcggtggg agaatgggt tcagatagc 
      tacagaggt gacggataa agccgggag aattactca 
      tacaattcc ggaagaaaa aacattggg ttacggttt 
      atatcctat aaagatggt ggtaagggg tgatcacac 
      ctctgtcga tttttttat ggctgtagt tcctccaaa 
      aaccgaatg tgtggtgtt tagtattga tgggtcttt 
      tactgccag tatgtcagt gacgggaag ggatccgaa 
      ggttagagc gggggccgt tgatgtcgt tagacattg 
      gcaagagct tggttacct catcgttta cataactgg 
      aagacctga catgcagca ccgaagagt tgtgtcatg 
      gttatccca acataattt ccctcatgg tctcgtgtc 
      atttgctga tggtctttc aaccgatcc tttaggaca 
      taaggctgc ttatctcaa taacttaat ccaaaatca 
      tggtgtaat tatccattg tgcatacgg tagcagtaa 
      agacataag tgtatatac ctcagtcgg actgaaacc 
      taaacaaat cttcatcgt cggcaaagc gtcaaagaa 
      acaacatgc tcaagcaac aatccgagt agacggcta 
      tgccgccca tctcaccat gctgatggc ccatggggg 
      ctcccgacc tgcgcaagg aaacgcccg aatggttct 
      accacgcgg ctatttcgg tctgccagg ccaaagttt 
      taaccgaag gagaaggta atcttaaca tttcgaaaa 
      gttgatgct tgatttact caaatgggt tacttttct 
      attacaaat cgaatggta gggtagttt aatatgcat 
      gtggtggtg tcactgttt cagccacga cacgagcat 
      ctcatctcc tgcgcgcaa cagtggctt tctgaagcg 
      caaagttta cattagtca tgtctaaca ttgacgata 
      gttacttag gatatggta attatttca acaaccttg 
      cactcacca gtagataat tcgcaccac atatttgtc 
      agtttgccg agatttgtg gtactaata agtacactt 
      aaagaaata actaaataa tatctcatt aataaaaat 
      ataaatgaa aa
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