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Gene information
General information
TC003032 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3954675..3990776
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06910

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC003032 RA
    CDS sequence
    • >TC003032 RA
      atgatttcc gccggtttg gagggacga ttgccctat 
      atgacctac ccagagcct tggcgtggg ccgcccaag 
      cagccaccg gaatttctt aggcctgct cccaagcca 
      gtgcccccg actagccgt tacaatggg gtgagcaca 
      ccgccgaac ggcatgcgt ctggctccc tcccagcac 
      tccctcagc aagtaccct tcgcccaca tcgataaca 
      acccccgtc aacctcacc caccagaag gatgaaatc 
      gtcgaagag cttccatac taccgcctc tacacagcc 
      ccaccgcac ctcccgcac ttcccccaa ttcaccacc 
      atgtaccag aacccctac acccccata atccgctcg 
      acctttccc accacctcc ctgccgccg ttggagacg 
      tactctccg accactccc acagcgatc acttcttca 
      acgttccta tcgcccccg tccacgttc agtcctccg 
      tcaccggtg aaacctggc caaagtgtt ctaagagat 
      tcgagggtt ccgacccag acgacagca caaatccag 
      tccaaccat cagtcagta gccttcaag gtgccctcc 
      ggcaaggag ggctcgctg aagcacagg atacttaca 
      aggccggag gatggcagc cgaaatctt gcaccctta 
      gatttacaa aagcctgcg gaaggacag cggaaaagg 
      ttcaatgcg accatgtcc ccgcccagg tcgcccaag 
      aaacctatt aataataat aacacagtt ccgagcaat 
      ttcacgaaa ggatctctt atacaactt gccaatggc 
      gagctgcgc aaaatcgag gacatgagg accgaggac 
      tttgtctcc tcggcggaa cacagcccc gaattgagg 
      cttgcggag agcacagtg gtgaggata gaggagaac 
      cacgccaca ggaactgcc accattacg cttagttac 
      aatcagaga cgcacacag gttgaagtg gagtcaacg 
      gtggagcac ccgttcttc gtgttcggc caaggctgg 
      gcctcgtac aatccggaa aaaacccac cagtgctac 
      ggactgaaa gtgcaccgc ttgcaagtg ggcgatatt 
      ttgatctcg ctgacacct cgggagcac tttggcggg 
      cttccgtcg acccgcagc acgaccatc atgacgacg 
      gccaccagc acagcgatg acagtgcgc cccctgccc 
      ttggacagc gtcccgaaa atgcaccac gacaaaacc 
      gaccaaaca agcaacagt caaatgcac ccaatcaac 
      ctgcacgca ctgtcgccc gaggggcgc aaaaggtcc 
      gcccttgaa caaatctgt gatgacgac gaacagcac 
      tcgaggaga agagtcgaa tga
    mRNA sequence
    • >TC003032 RA
      ccattcgtt gcaagcggc taatgtttt ccgtctacc 
      gaccacaga catgtccaa aaccgtacc caaaatagt 
      tccacgctt gtccagcgt tcgtttcgt cttgcgttc 
      cgcaggcag aggcgcgct atctcatcc actcatctt 
      caaaacatc cacacgccc tgaactttc ttaccgcag 
      ccgatcgtt caagttttg cctcacgaa agcagtttc 
      catgcacag ccttttacc aggtccact cctcgtagt 
      atccccgct acattcctg tagtagcgt agtagtaaa 
      gtgtttgcg tacgcactc agtaaaatc gagcactag 
      gaacagttg atacgtaat tcggtccag aaagaagct 
      aacaagtgc cggcttttt cccaaaaaa tgactcttt 
      gctttcgac aggaggtga tgtgtttgt ggtagcttt 
      gtggccatt tggagcaac tctcggggc ggacgcccg 
      agcgcactt cggagacga ctcaaaagg gcggcgagg 
      aaactcggt gccgatgat ttccgccgg tttggaggg 
      acgattgcc ctatatgac ctacccaga gccttggcg 
      tgggccgcc caagcagcc accggaatt tcttaggcc 
      tgctcccaa gccagtgcc cccgactag ccgttacaa 
      tggggtgag cacaccgcc gaacggcat gcgtctggc 
      tccctccca gcactccct cagcaagta cccttcgcc 
      cacatcgat aacaacccc cgtcaacct cacccacca 
      gaaggatga aatcgtcga agagcttcc atactaccg 
      cctctacac agccccacc gcacctccc gcacttccc 
      ccaattcac caccatgta ccagaaccc ctacacccc 
      cataatccg ctcgacctt tcccaccac ctccctgcc 
      gccgttgga gacgtactc tccgaccac tcccacagc 
      gatcacttc ttcaacgtt cctatcgcc cccgtccac 
      gttcagtcc tccgtcacc ggtgaaacc tggccaaag 
      tgttctaag agattcgag ggttccgac ccagacgac 
      agcacaaat ccagtccaa ccatcagtc agtagcctt 
      caaggtgcc ctccggcaa ggagggctc gctgaagca 
      caggatact tacaaggcc ggaggatgg cagccgaaa 
      tcttgcacc cttagattt acaaaagcc tgcggaagg 
      acagcggaa aaggttcaa tgcgaccat gtccccgcc 
      caggtcgcc caagaaacc tattaataa taataacac 
      agttccgag caatttcac gaaaggatc tcttataca 
      acttgccaa tggcgagct gcgcaaaat cgaggacat 
      gaggaccga ggactttgt ctcctcggc ggaacacag 
      ccccgaatt gaggcttgc ggagagcac agtggtgag 
      gatagagga gaaccacgc cacaggaac tgccaccat 
      tacgcttag ttacaatca gagacgcac acaggttga 
      agtggagtc aacggtgga gcacccgtt cttcgtgtt 
      cggccaagg ctgggcctc gtacaatcc ggaaaaaac 
      ccaccagtg ctacggact gaaagtgca ccgcttgca 
      agtgggcga tattttgat ctcgctgac acctcggga 
      gcactttgg cgggcttcc gtcgacccg cagcacgac 
      catcatgac gacggccac cagcacagc gatgacagt 
      gcgccccct gcccttgga cagcgtccc gaaaatgca 
      ccacgacaa aaccgacca aacaagcaa cagtcaaat 
      gcacccaat caacctgca cgcactgtc gcccgaggg 
      gcgcaaaag gtccgccct tgaacaaat ctgtgatga 
      cgacgaaca gcactcgag gagaagagt cgaatgagt 
      cgcggtggc gtgaaaaac tatgcattc gctgtggga 
      tagactgtt taacactag atcaatgct tctgaaatg 
      atattatcc cgccttgtt agctcgtgt tgaaataac 
      ccattaagc gaccacagt tgactcatg caaattcga 
      tacttgcta tttacatgc ttgagacgc actcggatc 
      ggtgttgcg acacgttct ggctggaaa gaactgttc 
      tttcaactt gcacgctgg tcgcccgaa tgatattac 
      acactgtat atttgtaaa taacgttgc gtaaagggt 
      tctttcaaa attagcgag ttcgtgttg ttttgcact 
      tttattcac tgtcagaag tccaaacaa cataattaa 
      tgtgctagt gcaatggaa ttatctaaa atttagatt 
      tctcatgac aatataaga cagggcagg atgtagcaa 
      acgtcgctg tgccgatta tgaactgga gtattatgt 
      agagtgata tgtactgac aagaacagt tgttactgg 
      aagttttga atacgtaga ggactatgg tagtaggaa 
      tctgctact gtcgatctc aaagagttg tgtgataga 
      aatgctttt aatgttgtt cacaaagat ctgtatttt 
      atttactat gaaatgatc atgcacgaa ctgatgtga 
      agcaacagc gccgctgtc cggtgactg gaaggcggc 
      gccgctgct gctaattaa tatatctag cgttcgtta 
      cttatgtaa actttttac tctagagta ttgaagatt 
      aacaattga ttatcttga gtgagagga atcaaacct 
      tatgctttt taatgttgt ttatattga tttccatgt 
      gaattcttg gctgtgata cagtaaagt atgtggaat 
      gaacgacac ttccctcca tgtctttat tttaaagat 
      cgtaactat aaagtgtga attgtacgt tattattag 
      attatcgta acatatacc tcactctgt gcttttcta 
      cattgacgg ttatcctta atgcttgta attcagatc 
      gttcaaatt tggtgacaa gaattgtta tttattgtg 
      tatatacag aatgtctta gataaattc aatttaatg 
      ttaagttaa ttaagtgaa ggaggggtt aaatttaac 
      cgcgcccga aaatgtaaa tatgccatg attgattaa 
      tttctcgtt tgttaagtt tgacgtcct ccgcaatgt 
      tttacaatt aaatgtgat ttaatgtgt aatttgttg 
      ggcattctg tgatatttg ccttcaaaa ttttacggt 
      ttcgtaatc agtattctg aaaggtcat tgttatgag 
      tggttcatt ttatttacc agttctttt tgccaaaac 
      tttcggtaa gctcaacta aattagcaa cagagttac 
      aatgttatt gcgttaatt tagaacaat ttgaaatgg 
      gtaggaaac tacatctaa ttaaatgaa gagaagaca 
      atgattatt gaagtgcct tgaatgaag gtttctcct 
      tcaagaatg ttatttacc tatgtagag gatctatga 
      aacaagcta acagatatg tacagatac atatatatt 
      attttacag atacatgta cagtgtgcc atgttgttg 
      aattgtaag gccggatgg cagtgcagt caaaccaat 
      aaaatatat aatatgta

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY