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Gene information
General information
TC011352 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4275739..4276928
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011352
    iBeetle screen iB_06908

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011352 RA
      MPKVKPEKK SRIHAAVAK QGITFNKSF GQHILKNPL 
      VITSMLDKA ALKPSDTVL EIGPGTGNL TLKLLESVK 
      QVVACEIDH RLVAELQKR VQNSPFKSK LQILIGDVL 
      KTELPFFSV CVANIPYQI SSPLVFKLL LHRPFFRCA 
      VLMFQKEFA DRLVAVPGD KLYCRLSIN TQLLARVDM 
      LMKVGKNNF RPPPKVESA VVRIEPRNP PPPISYTEW 
      DGLTRIAFS RKNKTLGAA FRQSVVLAT LEKNYKSFC 
      SVNNKEIPP GFNIKEKVE QVLKQVEGD QKRARTMDI 
      DDFMVLLHA FNSEGIHFS
    CDS sequence
    • >TC011352 RA
      atgccaaaa gtgaaaccg gagaaaaaa tcgcgtatt 
      cacgccgct gtggccaaa caaggcatc acattcaac 
      aagtccttc ggtcagcac atcctcaag aaccccctt 
      gtcataacc tcaatgttg gacaaggcg gcccttaag 
      ccctccgac accgtcctt gaaataggc cctgggacc 
      ggaaacttg actttaaag ctcctcgaa agtgttaaa 
      caggttgtc gcgtgtgaa attgaccat cgactggtg 
      gcagaattg caaaaaagg gtccagaat agccctttc 
      aagtccaaa ctccaaatc ctgattggg gacgttttg 
      aagacagaa ttgcccttt tttagcgtt tgcgtggct 
      aatatccct tatcaaatc tcgtcacct ttagtgttt 
      aagctactt ttgcaccgt ccgtttttt cggtgtgct 
      gttttaatg tttcaaaag gaatttgcc gatcggcta 
      gttgccgtc cctggagac aagctctac tgtcggttg 
      tctatcaac acgcagctt ctggcccgt gttgatatg 
      ttgatgaaa gtggggaaa aataatttt aggccccca 
      ccaaaggtt gagtcggct gtggttaga attgaacca 
      aggaatcca ccgccacct atttcttac acggagtgg 
      gatggactt acaagaatc gcattttcg cggaaaaat 
      aaaactttg ggagctgct tttagacaa tcggtggtc 
      ttggcaacg cttgagaag aattacaag tcgttttgt 
      agtgttaat aataaagaa atacccccc ggttttaat 
      attaaggaa aaagtggag caagtcttg aaacaagta 
      gaaggggac caaaagcgg gcccgcaca atggacatt 
      gatgatttt atggtgtta ttgcatgct tttaatagt 
      gaaggaata catttttca tga
    mRNA sequence
    • >TC011352 RA
      gcctataat acaaattgg cgtattgct agttgcctc 
      atcatgatc aaataaatg ctaaaattc ctgaataaa 
      cccatattt tgatttttg aacgttttg aatacattt 
      agtgacata atttggagt accctttgt gtctcttga 
      gtgcaattt taaagttta atttgtgtg accacatgt 
      cagtgtcac agtaacctc aaaatgcca aaagtgaaa 
      ccggagaaa aaatcgcgt attcacgcc gctgtggcc 
      aaacaaggc atcacattc aacaagtcc ttcggtcag 
      cacatcctc aagaacccc cttgtcata acctcaatg 
      ttggacaag gcggccctt aagccctcc gacaccgtc 
      cttgaaata ggccctggg accggaaac ttgacttta 
      aagctcctc gaaagtgtt aaacaggtt gtcgcgtgt 
      gaaattgac catcgactg gtggcagaa ttgcaaaaa 
      agggtccag aatagccct ttcaagtcc aaactccaa 
      atcctgatt ggggacgtt ttgaagaca gaattgccc 
      ttttttagc gtttgcgtg gctaatatc ccttatcaa 
      atctcgtca cctttagtg tttaagcta cttttgcac 
      cgtccgttt tttcggtgt gctgtttta atgtttcaa 
      aaggaattt gccgatcgg ctagttgcc gtccctgga 
      gacaagctc tactgtcgg ttgtctatc aacacgcag 
      cttctggcc cgtgttgat atgttgatg aaagtgggg 
      aaaaataat tttaggccc ccaccaaag gttgagtcg 
      gctgtggtt agaattgaa ccaaggaat ccaccgcca 
      cctatttct tacacggag tgggatgga cttacaaga 
      atcgcattt tcgcggaaa aataaaact ttgggagct 
      gcttttaga caatcggtg gtcttggca acgcttgag 
      aagaattac aagtcgttt tgtagtgtt aataataaa 
      gaaataccc cccggtttt aatattaag gaaaaagtg 
      gagcaagtc ttgaaacaa gtagaaggg gaccaaaag 
      cgggcccgc acaatggac attgatgat tttatggtg 
      ttattgcat gcttttaat agtgaagga atacatttt 
      tcatgaaat aaagttttt tattcctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY