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Gene information
General information
TC003135 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1269872..1292795
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003135
    iBeetle screen iB_06891

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003135 RA
      MVRVTMLLF TALFFLEKC WFCTCLVIN PGSSGGLTS 
      GDPAPGRSP GPPEIELTT LLLPQEADE CGLEGNVQL 
      ATENFINLT LGHRLFITD HLVAGMEYQ VNLKRIREG 
      PVITFSIKS TVDGVPKGK FINTIDELN CWPNVFKSH 
      LNGSALTLK WTMEDSEEL AKTNLTFEV NYQVSDDPS 
      VYRVIYSFP ISQDCQLRL KKEEAVISW RDFPHSISD 
      CQVWFPSHD TGHSLVIEL TRLNVPCSK GYIHFSGLN 
      ITQHQQFRS HRQAHLCGK LEELPESDR NVYFPFSHT 
      PPVMHTHGN PVFALSYHL VDYCYNVTF VARNGSFEL 
      KPTEELQCT FKIYLPYGN RVALNLQIG DSTSTGTPA 
      TDALLQNPK SGEKCDGLL TQLHDGDNT WSHCTKPGD 
      AERRIEIVS RGNKVVLRV NVRSSNRGS LGLRMMYHA 
      TPIEEIVGA CGFGWVTLK QFCVTAVEE FKLPWAQAE 
      MECIRRGGH LASIRSDYA QNIINNLLL NSPGYRDHN 
      AYWIGASDK IVEGDFRWS DGLPFSFTS EYNAD
    CDS sequence
    • >TC003135 RA
      atggtgaga gtgaccatg ttgctgttt acggcactt 
      tttttccta gaaaagtgc tggttctgt acttgtctt 
      gtgattaat ccggggagt tccggcggc ctcaccagc 
      ggcgatccc gctccgggg cgctcgccg ggacccccg 
      gaaatcgag ctcaccacg ctgcttctg ccccaagaa 
      gcggacgag tgcggactt gaaggaaac gttcaactt 
      gcaacggaa aattttatc aatcttacg ctggggcac 
      agacttttt attacggac cacttagtc gcaggaatg 
      gagtaccaa gttaacttg aagaggatc cgagaagga 
      cctgtcata acattctcg ataaaatca acagttgat 
      ggcgttcca aagggaaaa tttatcaac acaatcgac 
      gaattaaac tgttggccg aacgtcttt aaaagtcac 
      ttgaatggg tcggcatta acgctcaag tggacgatg 
      gaagacagc gaggagttg gcgaaaacg aatttaact 
      ttcgaagtg aattaccaa gtgtcagac gatccatct 
      gtctacaga gtgatatat tcttttcca ataagtcaa 
      gattgccaa ctccgcctg aaaaaagag gaagccgtg 
      ataagttgg cgagatttt ccccattcc atcagcgac 
      tgtcaagtg tggtttccc agccacgat acaggccac 
      agtctcgtc atcgaactc acaagactc aatgtgccc 
      tgctctaaa ggctacatc cacttttcc ggcctcaac 
      atcacgcaa caccagcag ttccggagc caccggcaa 
      gcgcatctc tgcggaaag ctggaggaa ctgccggag 
      agcgaccgg aatgtctac ttccccttt tcccatact 
      cctccggtt atgcacact catggtaat ccggtcttc 
      gcgttatcc taccatctg gtcgattac tgctacaat 
      gtcactttt gtcgcaaga aacggctcc ttcgaactc 
      aagcctacg gaggagctg cagtgcact ttcaaaatt 
      tatctgccg tatgggaac agagtggcg ctaaatctg 
      cagatcggg gacagcact tccacaggg actcccgcg 
      acggacgcc cttctgcag aatccgaag agcggcgag 
      aaatgtgat ggtttactg acgcaactc cacgacgga 
      gacaacact tggtcccac tgcactaag cccggagac 
      gccgagcgt cggattgaa attgtatca agagggaat 
      aaggtggtg ctgcgggta aatgtgcgt agttctaat 
      aggggttct ttgggatta cggatgatg tatcatgct 
      acaccgatt gaggagatc gtgggtgcg tgcggattc 
      gggtgggtg actcttaag cagttttgt gtgacggct 
      gtggaggag tttaaactg ccatgggct caggcagaa 
      atggagtgc atcaggagg ggaggacac ctggcgagc 
      atcaggagc gactacgca cagaacatc atcaacaac 
      ctgcttctg aatagcccc gggtatcgg gaccacaac 
      gcttattgg atcggtgcg tctgataaa atcgtcgag 
      ggtgacttc cggtggtcc gacggcctc cccttctcg 
      ttcacgagt gagtataac gcggattag
    mRNA sequence
    • >TC003135 RA
      aatgcgtcc gaacggttt ttacgcgga tgttaatcg 
      cttcttgta atattattt ccaagttta gatgttacg 
      agccttttg ttgttgccc gctattttt cgctcatga 
      gtgcggaat ggtgagagt gaccatgtt gctgtttac 
      ggcactttt tttcctaga aaagtgctg gttctgtac 
      ttgtcttgt gattaatcc ggggagttc cggcggcct 
      caccagcgg cgatcccgc tccggggcg ctcgccggg 
      acccccgga aatcgagct caccacgct gcttctgcc 
      ccaagaagc ggacgagtg cggacttga aggaaacgt 
      tcaacttgc aacggaaaa ttttatcaa tcttacgct 
      ggggcacag actttttat tacggacca cttagtcgc 
      aggaatgga gtaccaagt taacttgaa gaggatccg 
      agaaggacc tgtcataac attctcgat aaaatcaac 
      agttgatgg cgttccaaa gggaaaatt tatcaacac 
      aatcgacga attaaactg ttggccgaa cgtctttaa 
      aagtcactt gaatgggtc ggcattaac gctcaagtg 
      gacgatgga agacagcga ggagttggc gaaaacgaa 
      tttaacttt cgaagtgaa ttaccaagt gtcagacga 
      tccatctgt ctacagagt gatatattc ttttccaat 
      aagtcaaga ttgccaact ccgcctgaa aaaagagga 
      agccgtgat aagttggcg agattttcc ccattccat 
      cagcgactg tcaagtgtg gtttcccag ccacgatac 
      aggccacag tctcgtcat cgaactcac aagactcaa 
      tgtgccctg ctctaaagg ctacatcca cttttccgg 
      cctcaacat cacgcaaca ccagcagtt ccggagcca 
      ccggcaagc gcatctctg cggaaagct ggaggaact 
      gccggagag cgaccggaa tgtctactt ccccttttc 
      ccatactcc tccggttat gcacactca tggtaatcc 
      ggtcttcgc gttatccta ccatctggt cgattactg 
      ctacaatgt cacttttgt cgcaagaaa cggctcctt 
      cgaactcaa gcctacgga ggagctgca gtgcacttt 
      caaaattta tctgccgta tgggaacag agtggcgct 
      aaatctgca gatcgggga cagcacttc cacagggac 
      tcccgcgac ggacgccct tctgcagaa tccgaagag 
      cggcgagaa atgtgatgg tttactgac gcaactcca 
      cgacggaga caacacttg gtcccactg cactaagcc 
      cggagacgc cgagcgtcg gattgaaat tgtatcaag 
      agggaataa ggtggtgct gcgggtaaa tgtgcgtag 
      ttctaatag gggttcttt gggattacg gatgatgta 
      tcatgctac accgattga ggagatcgt gggtgcgtg 
      cggattcgg gtgggtgac tcttaagca gttttgtgt 
      gacggctgt ggaggagtt taaactgcc atgggctca 
      ggcagaaat ggagtgcat caggagggg aggacacct 
      ggcgagcat caggagcga ctacgcaca gaacatcat 
      caacaacct gcttctgaa tagccccgg gtatcggga 
      ccacaacgc ttattggat cggtgcgtc tgataaaat 
      cgtcgaggg tgacttccg gtggtccga cggcctccc 
      cttctcgtt cacgagtga gtataacgc ggattagcg 
      ttcatcaac tactccaaa taaatcgta ttctaagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY